Restriction map for
pF1KA1372
(Linear) (Six-base) MAPSORT of: KIAA1372.seq Check: 79 from: 1 to: 2247
KIAA1372.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 236 2200 2247
Size: 236 1964 47
Fragments arranged by size:
1964 236 47
AatII G_ACGT'C
Cuts at: 0 1673 2247
Size: 1673 574
Acc65I G'GTAC_C
Cuts at: 0 1862 2247
Size: 1862 385
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 544 570 787 2247
Size: 82 462 26 217 1460
Fragments arranged by size:
1460 462 217 82 26
AfeI AGC'GCT
Cuts at: 0 1006 2247
Size: 1006 1241
AflIII A'CryG_T
Cuts at: 0 1447 2247
Size: 1447 800
AhdI GACnn_n'nnGTC
Cuts at: 0 12 2247
Size: 12 2235
AleI CACnn'nnGTG
Cuts at: 0 1882 2247
Size: 1882 365
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 691 723 2247
Size: 691 32 1524
Fragments arranged by size:
1524 691 32
AlwNI CAG_nnn'CTG
Cuts at: 0 429 630 2247
Size: 429 201 1617
Fragments arranged by size:
1617 429 201
ApaI G_GGCC'C
Cuts at: 0 1985 2247
Size: 1985 262
ApaLI G'TGCA_C
Cuts at: 0 1085 1747 2247
Size: 1085 662 500
Fragments arranged by size:
1085 662 500
AvaI C'yCGr_G
Cuts at: 0 285 345 856 956 1061 1504 1849 1880
Size: 285 60 511 100 105 443 345 31
Cuts at: 1880 1984 2247
Size: 104 263
Fragments arranged by size:
511 443 345 285 263 105 104 100
60 31
BanI G'GyrC_C
Cuts at: 0 739 1649 1862 2247
Size: 739 910 213 385
Fragments arranged by size:
910 739 385 213
BanII G_rGCy'C
Cuts at: 0 866 1030 1465 1850 1985 2002 2024 2247
Size: 866 164 435 385 135 17 22 223
Fragments arranged by size:
866 435 385 223 164 135 22 17
BbeI G_GCGC'C
Cuts at: 0 1653 2247
Size: 1653 594
BbsI GAAGACnn'nnnn_
Cuts at: 0 1055 1397 1754 2171 2235 2247
Size: 1055 342 357 417 64 12
Fragments arranged by size:
1055 417 357 342 64 12
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 81 115 1528 1562 2247
Size: 81 34 1413 34 685
Fragments arranged by size:
1413 685 81 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1025 2247
Size: 1025 1222
BlpI GC'TnA_GC
Cuts at: 0 1373 1582 2247
Size: 1373 209 665
Fragments arranged by size:
1373 665 209
Bme1580I G_kGCm'C
Cuts at: 0 811 1089 1751 1985 2247
Size: 811 278 662 234 262
Fragments arranged by size:
811 662 278 262 234
BmrI ACTGGGnnnn_n'
Cuts at: 0 18 2154 2247
Size: 18 2136 93
Fragments arranged by size:
2136 93 18
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 636 2016 2247
Size: 636 1380 231
Fragments arranged by size:
1380 636 231
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 1775 2174 2247
Size: 322 1453 399 73
Fragments arranged by size:
1453 399 322 73
Bpu10I CC'TnA_GC
Cuts at: 0 1001 2247
Size: 1001 1246
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 814 2247
Size: 166 648 1433
Fragments arranged by size:
1433 648 166
BsaI GGTCTCn'nnnn_
Cuts at: 0 523 2247
Size: 523 1724
BsaHI Gr'CG_yC
Cuts at: 0 577 721 1650 1670 2247
Size: 577 144 929 20 577
Fragments arranged by size:
929 577 577 144 20
BsaWI w'CCGG_w
Cuts at: 0 338 2012 2247
Size: 338 1674 235
Fragments arranged by size:
1674 338 235
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 691 721 937 967 1906 1936 2247
Size: 691 30 216 30 939 30 311
Fragments arranged by size:
939 691 311 216 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 223 811 874 942 976 986 1261 1291
Size: 223 588 63 68 34 10 275 30
Cuts at: 1291 1294 1333 1336 2247
Size: 3 39 3 911
Fragments arranged by size:
911 588 275 223 68 63 39 34
30 10 3 3
BseYI C'CCAG_C
Cuts at: 0 429 743 2247
Size: 429 314 1504
Fragments arranged by size:
1504 429 314
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 772 2014 2108 2247
Size: 772 1242 94 139
Fragments arranged by size:
1242 772 139 94
BsiHKAI G_wGCw'C
Cuts at: 0 182 602 1089 1751 2247
Size: 182 420 487 662 496
Fragments arranged by size:
662 496 487 420 182
BsmBI CGTCTCn'nnnn_
Cuts at: 0 954 1677 2247
Size: 954 723 570
Fragments arranged by size:
954 723 570
Bsp1286I G_dGCh'C
Cuts at: 0 182 602 811 866 1030 1089 1465 1751
Size: 182 420 209 55 164 59 376 286
Cuts at: 1751 1850 1985 2002 2024 2247
Size: 99 135 17 22 223
Fragments arranged by size:
420 376 286 223 209 182 164 135
99 59 55 22 17
BspEI T'CCGG_A
Cuts at: 0 2012 2247
Size: 2012 235
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 236 782 1643 2200 2247
Size: 236 546 861 557 47
Fragments arranged by size:
861 557 546 236 47
BsrBI CCG'CTC
Cuts at: 0 1405 1443 1469 2247
Size: 1405 38 26 778
Fragments arranged by size:
1405 778 38 26
BsrDI GCAATG_nn'
Cuts at: 0 50 2223 2247
Size: 50 2173 24
Fragments arranged by size:
2173 50 24
BsrFI r'CCGG_y
Cuts at: 0 1961 2247
Size: 1961 286
BsrGI T'GTAC_A
Cuts at: 0 1163 2247
Size: 1163 1084
BstAPI GCAn_nnn'nTGC
Cuts at: 0 539 2247
Size: 539 1708
BstBI TT'CG_AA
Cuts at: 0 1901 2247
Size: 1901 346
BstXI CCAn_nnnn'nTGG
Cuts at: 0 300 2247
Size: 300 1947
BstYI r'GATC_y
Cuts at: 0 357 815 923 1658 2247
Size: 357 458 108 735 589
Fragments arranged by size:
735 589 458 357 108
Bsu36I CC'TnA_GG
Cuts at: 0 1934 2247
Size: 1934 313
BtgI C'CryG_G
Cuts at: 0 251 1713 2247
Size: 251 1462 534
Fragments arranged by size:
1462 534 251
BtsI GCAGTG_nn'
Cuts at: 0 55 324 652 1128 1612 1743 2247
Size: 55 269 328 476 484 131 504
Fragments arranged by size:
504 484 476 328 269 131 55
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 869 904 2247
Size: 869 35 1343
Fragments arranged by size:
1343 869 35
EaeI y'GGCC_r
Cuts at: 0 491 1710 1716 2247
Size: 491 1219 6 531
Fragments arranged by size:
1219 531 491 6
EarI CTCTTCn'nnn_
Cuts at: 0 451 788 1325 1334 1899 2007 2247
Size: 451 337 537 9 565 108 240
Fragments arranged by size:
565 537 451 337 240 108 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 328 842 1681 2247
Size: 328 514 839 566
Fragments arranged by size:
839 566 514 328
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 322 544 570 787 1775 2174 2247
Size: 82 240 222 26 217 988 399 73
Fragments arranged by size:
988 399 240 222 217 82 73 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2032 2247
Size: 2032 215
EcoO109I rG'GnC_Cy
Cuts at: 0 66 390 1108 1409 1981 2247
Size: 66 324 718 301 572 266
Fragments arranged by size:
718 572 324 301 266 66
FspI TGC'GCA
Cuts at: 0 1639 2247
Size: 1639 608
HaeII r_GCGC'y
Cuts at: 0 1008 1653 1805 2247
Size: 1008 645 152 442
Fragments arranged by size:
1008 645 442 152
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 30 268 300 903 915 935 947 1657
Size: 30 238 32 603 12 20 12 710
Cuts at: 1657 1689 2170 2202 2247
Size: 32 481 32 45
Fragments arranged by size:
710 603 481 238 45 32 32 32
30 20 12 12
HincII GTy'rAC
Cuts at: 0 549 1488 2247
Size: 549 939 759
Fragments arranged by size:
939 759 549
HpaI GTT'AAC
Cuts at: 0 1488 2247
Size: 1488 759
KasI G'GCGC_C
Cuts at: 0 1649 2247
Size: 1649 598
KpnI G_GTAC'C
Cuts at: 0 1866 2247
Size: 1866 381
MscI TGG'CCA
Cuts at: 0 493 1718 2247
Size: 493 1225 529
Fragments arranged by size:
1225 529 493
MslI CAynn'nnrTG
Cuts at: 0 1418 1882 2247
Size: 1418 464 365
Fragments arranged by size:
1418 464 365
MspA1I CmG'CkG
Cuts at: 0 296 309 426 678 1968 2247
Size: 296 13 117 252 1290 279
Fragments arranged by size:
1290 296 279 252 117 13
NarI GG'CG_CC
Cuts at: 0 1650 2247
Size: 1650 597
NcoI C'CATG_G
Cuts at: 0 251 2247
Size: 251 1996
NspI r_CATG'y
Cuts at: 0 192 542 1451 2247
Size: 192 350 909 796
Fragments arranged by size:
909 796 350 192
PasI CC'CwG_GG
Cuts at: 0 24 1973 2247
Size: 24 1949 274
Fragments arranged by size:
1949 274 24
PciI A'CATG_T
Cuts at: 0 1447 2247
Size: 1447 800
PflMI CCAn_nnn'nTGG
Cuts at: 0 330 1705 2238 2247
Size: 330 1375 533 9
Fragments arranged by size:
1375 533 330 9
PfoI T'CCnGG_A
Cuts at: 0 353 715 1475 1661 2247
Size: 353 362 760 186 586
Fragments arranged by size:
760 586 362 353 186
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 691 723 2247
Size: 691 32 1524
Fragments arranged by size:
1524 691 32
PpuMI rG'GwC_Cy
Cuts at: 0 390 1108 1409 2247
Size: 390 718 301 838
Fragments arranged by size:
838 718 390 301
PspOMI G'GGCC_C
Cuts at: 0 1981 2247
Size: 1981 266
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1846 1878 2247
Size: 1846 32 369
Fragments arranged by size:
1846 369 32
PstI C_TGCA'G
Cuts at: 0 647 1559 1738 2211 2247
Size: 647 912 179 473 36
Fragments arranged by size:
912 647 473 179 36
PvuII CAG'CTG
Cuts at: 0 296 309 426 2247
Size: 296 13 117 1821
Fragments arranged by size:
1821 296 117 13
SanDI GG'GwC_CC
Cuts at: 0 1409 2247
Size: 1409 838
SapI GCTCTTCn'nnn_
Cuts at: 0 451 2007 2247
Size: 451 1556 240
Fragments arranged by size:
1556 451 240
SbfI CC_TGCA'GG
Cuts at: 0 1559 2211 2247
Size: 1559 652 36
Fragments arranged by size:
1559 652 36
SexAI A'CCwGG_T
Cuts at: 0 1481 2247
Size: 1481 766
SfcI C'TryA_G
Cuts at: 0 643 1555 1734 2077 2207 2247
Size: 643 912 179 343 130 40
Fragments arranged by size:
912 643 343 179 130 40
SfoI GGC'GCC
Cuts at: 0 1651 2247
Size: 1651 596
SmaI CCC'GGG
Cuts at: 0 347 958 1063 2247
Size: 347 611 105 1184
Fragments arranged by size:
1184 611 347 105
SmlI C'TyrA_G
Cuts at: 0 181 829 2247
Size: 181 648 1418
Fragments arranged by size:
1418 648 181
SspI AAT'ATT
Cuts at: 0 36 2247
Size: 36 2211
StuI AGG'CCT
Cuts at: 0 565 967 1175 1723 2247
Size: 565 402 208 548 524
Fragments arranged by size:
565 548 524 402 208
StyI C'CwwG_G
Cuts at: 0 251 1184 1587 2247
Size: 251 933 403 660
Fragments arranged by size:
933 660 403 251
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 757 2247
Size: 757 1490
TatI w'GTAC_w
Cuts at: 0 185 1163 2247
Size: 185 978 1084
Fragments arranged by size:
1084 978 185
XbaI T'CTAG_A
Cuts at: 0 2005 2247
Size: 2005 242
XmaI C'CCGG_G
Cuts at: 0 345 956 1061 2247
Size: 345 611 105 1186
Fragments arranged by size:
1186 611 345 105
XmnI GAAnn'nnTTC
Cuts at: 0 130 2247
Size: 130 2117
ZraI GAC'GTC
Cuts at: 0 1671 2247
Size: 1671 576
Enzymes that do cut:
AarI AatII Acc65I AcuI AfeI AflIII AhdI AleI
AloI AlwNI ApaI ApaLI AvaI BanI BanII BbeI
BbsI BcgI BglI BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BsrGI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtsI CspCI EaeI EarI EciI Eco57MI EcoNI EcoO109I
FspI HaeII Hin4I HincII HpaI KasI KpnI MscI
MslI MspA1I NarI NcoI NspI PasI PciI PflMI
PfoI PpiI PpuMI PspOMI PsrI PstI PvuII SanDI
SapI SbfI SexAI SfcI SfoI SmaI SmlI SspI
StuI StyI TaqII TatI XbaI XmaI XmnI ZraI
Enzymes that do not cut:
AccI AclI AflII AgeI AlfI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbvCI BciVI BclI BfrBI
BglII BmgBI BmtI BsaAI BsaBI BsiEI BsiWI BsmI
BspHI BssHII BssSI BstEII BstZ17I BtgZI ClaI DraI
DraIII DrdI EagI EcoICRI EcoRI EcoRV FalI FseI
FspAI HindIII MfeI MluI MmeI NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI PmeI PmlI PshAI
PsiI PspXI PvuI RsrII SacI SacII SalI ScaI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SwaI
TstI Tth111I XcmI XhoI