Restriction map for pF1KA1376
 (Linear) (Six-base) MAPSORT of: KIAA1376.seq  Check: 2481  from: 1  to: 1242



KIAA1376.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:08  ..

Acc65I G'GTAC_C

Cuts at:      0     928    1242 
   Size:        928     314
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     248    1049    1242 
   Size:        248     801     193

  Fragments arranged by size: 

                 801     248     193
 
AflII C'TTAA_G

Cuts at:      0     576    1242 
   Size:        576     666
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     350    1242 
   Size:        350     892
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     637     669    1242 
   Size:        637      32     573

  Fragments arranged by size: 

                 637     573      32
 
ApoI r'AATT_y

Cuts at:      0     102     143     463     806    1242 
   Size:        102      41     320     343     436

  Fragments arranged by size: 

                 436     343     320     102      41
 
BanI G'GyrC_C

Cuts at:      0     666     928    1242 
   Size:        666     262     314

  Fragments arranged by size: 

                 666     314     262
 
BanII G_rGCy'C

Cuts at:      0    1122    1242 
   Size:       1122     120
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      75    1242 
   Size:         75    1167
 
BclI T'GATC_A

Cuts at:      0    1203    1242 
   Size:       1203      39
 
BfrBI ATG'CAT

Cuts at:      0     337    1242 
   Size:        337     905
 
BglI GCCn_nnn'nGGC

Cuts at:      0     392    1242 
   Size:        392     850
 
Bme1580I G_kGCm'C

Cuts at:      0     272    1242 
   Size:        272     970
 
BmgBI CAC'GTC

Cuts at:      0     785    1242 
   Size:        785     457
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     125    1242 
   Size:        125    1117
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     666    1242 
   Size:        666     576
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     908    1242 
   Size:        908     334
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     948    1242 
   Size:        948     294
 
BsaBI GATnn'nnATC

Cuts at:      0    1196    1242 
   Size:       1196      46
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     181     211    1242 
   Size:        181      30    1031

  Fragments arranged by size: 

                1031     181      30
 
BseYI C'CCAG_C

Cuts at:      0       6    1039    1242 
   Size:          6    1033     203

  Fragments arranged by size: 

                1033     203       6
 
BsmI GAATG_Cn'

Cuts at:      0     337    1242 
   Size:        337     905
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      93     775    1242 
   Size:         93     682     467

  Fragments arranged by size: 

                 682     467      93
 
Bsp1286I G_dGCh'C

Cuts at:      0     272    1122    1242 
   Size:        272     850     120

  Fragments arranged by size: 

                 850     272     120
 
BsrDI GCAATG_nn'

Cuts at:      0      63    1242 
   Size:         63    1179
 
BsrFI r'CCGG_y

Cuts at:      0     193    1242 
   Size:        193    1049
 
BstBI TT'CG_AA

Cuts at:      0     380    1242 
   Size:        380     862
 
Bsu36I CC'TnA_GG

Cuts at:      0     561    1242 
   Size:        561     681
 
BtsI GCAGTG_nn'

Cuts at:      0     402    1242 
   Size:        402     840
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      58    1242 
   Size:         58    1184
 
EarI CTCTTCn'nnn_

Cuts at:      0      13     657    1242 
   Size:         13     644     585

  Fragments arranged by size: 

                 644     585      13
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1089    1242 
   Size:       1089     153
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     248     908    1049    1242 
   Size:        248     660     141     193

  Fragments arranged by size: 

                 660     248     193     141
 
EcoRV GAT'ATC

Cuts at:      0     489    1242 
   Size:        489     753
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      73     105    1210    1242 
   Size:         73      32    1105      32

  Fragments arranged by size: 

                1105      73      32      32
 
HindIII A'AGCT_T

Cuts at:      0      33    1242 
   Size:         33    1209
 
KpnI G_GTAC'C

Cuts at:      0     932    1242 
   Size:        932     310
 
MslI CAynn'nnrTG

Cuts at:      0     332     395     991    1242 
   Size:        332      63     596     251

  Fragments arranged by size: 

                 596     332     251      63
 
NaeI GCC'GGC

Cuts at:      0     195    1242 
   Size:        195    1047
 
NgoMIV G'CCGG_C

Cuts at:      0     193    1242 
   Size:        193    1049
 
NsiI A_TGCA'T

Cuts at:      0     339    1242 
   Size:        339     903
 
NspI r_CATG'y

Cuts at:      0     163    1242 
   Size:        163    1079
 
PfoI T'CCnGG_A

Cuts at:      0     885    1148    1242 
   Size:        885     263      94

  Fragments arranged by size: 

                 885     263      94
 
SapI GCTCTTCn'nnn_

Cuts at:      0     657    1242 
   Size:        657     585
 
SfcI C'TryA_G

Cuts at:      0     837    1242 
   Size:        837     405
 
SmlI C'TyrA_G

Cuts at:      0     576     963    1242 
   Size:        576     387     279

  Fragments arranged by size: 

                 576     387     279
 
SspI AAT'ATT

Cuts at:      0     859    1242 
   Size:        859     383
 
StuI AGG'CCT

Cuts at:      0     432     697    1242 
   Size:        432     265     545

  Fragments arranged by size: 

                 545     432     265
 
StyI C'CwwG_G

Cuts at:      0     433    1242 
   Size:        433     809
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     399    1053    1242 
   Size:        399     654     189

  Fragments arranged by size: 

                 654     399     189
 
XbaI T'CTAG_A

Cuts at:      0     184    1242 
   Size:        184    1058
 
XmnI GAAnn'nnTTC

Cuts at:      0     620    1242 
   Size:        620     622
 

 Enzymes that do cut:

   Acc65I     AcuI    AflII     AlfI     AloI     ApoI     BanI    BanII
    BciVI     BclI    BfrBI     BglI Bme1580I    BmgBI     BmrI     BplI
     BpmI    BpuEI    BsaBI    BsaXI    BseYI     BsmI    BsmBI Bsp1286I
    BsrDI    BsrFI    BstBI   Bsu36I     BtsI     DrdI     EarI     EciI
  Eco57MI    EcoRV    Hin4I  HindIII     KpnI     MslI     NaeI   NgoMIV
     NsiI     NspI     PfoI     SapI     SfcI     SmlI     SspI     StuI
     StyI    TaqII     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI   AflIII     AgeI     AhdI
     AleI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI    BglII
     BlpI     BmtI   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI    BseRI
     BsgI    BsiEI  BsiHKAI    BsiWI    BspEI    BspHI    BspMI    BsrBI
    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI  BstZ17I
     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     EaeI     EagI
  EcoICRI    EcoNI EcoO109I    EcoRI     FalI     FseI     FspI    FspAI
    HaeII   HincII     HpaI     KasI     MfeI     MluI     MmeI     MscI
   MspA1I     NarI     NcoI     NdeI     NheI     NotI     NruI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SwaI     TatI     TstI  Tth111I     XcmI     XhoI     XmaI     ZraI
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