Restriction map for
pF1KA1378
(Linear) (Six-base) MAPSORT of: KIAA1378.seq Check: 4099 from: 1 to: 1860
KIAA1378.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 323 611 1860
Size: 323 288 1249
Fragments arranged by size:
1249 323 288
AflIII A'CryG_T
Cuts at: 0 1071 1860
Size: 1071 789
AlwNI CAG_nnn'CTG
Cuts at: 0 904 1860
Size: 904 956
ApoI r'AATT_y
Cuts at: 0 1814 1860
Size: 1814 46
BamHI G'GATC_C
Cuts at: 0 1834 1860
Size: 1834 26
BanI G'GyrC_C
Cuts at: 0 1329 1860
Size: 1329 531
BbsI GAAGACnn'nnnn_
Cuts at: 0 362 614 1860
Size: 362 252 1246
Fragments arranged by size:
1246 362 252
BbvCI CC'TCA_GC
Cuts at: 0 725 1860
Size: 725 1135
BclI T'GATC_A
Cuts at: 0 1311 1860
Size: 1311 549
BfrBI ATG'CAT
Cuts at: 0 1696 1860
Size: 1696 164
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 639 1796 1860
Size: 639 1157 64
Fragments arranged by size:
1157 639 64
Bpu10I CC'TnA_GC
Cuts at: 0 725 1860
Size: 725 1135
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 906 1860
Size: 196 710 954
Fragments arranged by size:
954 710 196
BsaAI yAC'GTr
Cuts at: 0 1530 1860
Size: 1530 330
BsaBI GATnn'nnATC
Cuts at: 0 1445 1860
Size: 1445 415
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1769 1775 1799 1805 1860
Size: 1769 6 24 6 55
Fragments arranged by size:
1769 55 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1367 1547 1860
Size: 1367 180 313
Fragments arranged by size:
1367 313 180
BsmI GAATG_Cn'
Cuts at: 0 1015 1860
Size: 1015 845
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 432 675 1860
Size: 432 243 1185
Fragments arranged by size:
1185 432 243
BsrBI CCG'CTC
Cuts at: 0 1575 1860
Size: 1575 285
BsrDI GCAATG_nn'
Cuts at: 0 1407 1503 1698 1860
Size: 1407 96 195 162
Fragments arranged by size:
1407 195 162 96
BsrFI r'CCGG_y
Cuts at: 0 783 1860
Size: 783 1077
BstEII G'GTnAC_C
Cuts at: 0 988 1860
Size: 988 872
BstXI CCAn_nnnn'nTGG
Cuts at: 0 507 744 1845 1860
Size: 507 237 1101 15
Fragments arranged by size:
1101 507 237 15
BstYI r'GATC_y
Cuts at: 0 88 982 1753 1834 1860
Size: 88 894 771 81 26
Fragments arranged by size:
894 771 88 81 26
Bsu36I CC'TnA_GG
Cuts at: 0 1230 1860
Size: 1230 630
BtgI C'CryG_G
Cuts at: 0 1347 1860
Size: 1347 513
BtsI GCAGTG_nn'
Cuts at: 0 1764 1860
Size: 1764 96
EarI CTCTTCn'nnn_
Cuts at: 0 173 1482 1860
Size: 173 1309 378
Fragments arranged by size:
1309 378 173
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 577 1860
Size: 577 1283
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 323 611 639 1796 1860
Size: 323 288 28 1157 64
Fragments arranged by size:
1157 323 288 64 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1143 1175 1291 1323 1425 1457 1628 1660
Size: 1143 32 116 32 102 32 171 32
Cuts at: 1660 1860
Size: 200
Fragments arranged by size:
1143 200 171 116 102 32 32 32
32
HincII GTy'rAC
Cuts at: 0 402 1851 1860
Size: 402 1449 9
Fragments arranged by size:
1449 402 9
HindIII A'AGCT_T
Cuts at: 0 143 643 1510 1860
Size: 143 500 867 350
Fragments arranged by size:
867 500 350 143
MfeI C'AATT_G
Cuts at: 0 501 1250 1860
Size: 501 749 610
Fragments arranged by size:
749 610 501
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 725 1731 1860
Size: 725 1006 129
Fragments arranged by size:
1006 725 129
MslI CAynn'nnrTG
Cuts at: 0 1076 1385 1860
Size: 1076 309 475
Fragments arranged by size:
1076 475 309
NsiI A_TGCA'T
Cuts at: 0 1698 1860
Size: 1698 162
NspI r_CATG'y
Cuts at: 0 1075 1860
Size: 1075 785
PciI A'CATG_T
Cuts at: 0 1071 1860
Size: 1071 789
PflMI CCAn_nnn'nTGG
Cuts at: 0 707 1860
Size: 707 1153
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 76 108 1860
Size: 76 32 1752
Fragments arranged by size:
1752 76 32
PstI C_TGCA'G
Cuts at: 0 443 1771 1860
Size: 443 1328 89
Fragments arranged by size:
1328 443 89
SapI GCTCTTCn'nnn_
Cuts at: 0 1482 1860
Size: 1482 378
SfcI C'TryA_G
Cuts at: 0 439 1767 1860
Size: 439 1328 93
Fragments arranged by size:
1328 439 93
SmlI C'TyrA_G
Cuts at: 0 211 885 1860
Size: 211 674 975
Fragments arranged by size:
975 674 211
SnaBI TAC'GTA
Cuts at: 0 1530 1860
Size: 1530 330
SspI AAT'ATT
Cuts at: 0 669 1860
Size: 669 1191
StyI C'CwwG_G
Cuts at: 0 934 1220 1860
Size: 934 286 640
Fragments arranged by size:
934 640 286
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 790 1066 1860
Size: 790 276 794
Fragments arranged by size:
794 790 276
TatI w'GTAC_w
Cuts at: 0 1321 1860
Size: 1321 539
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1775 1807 1860
Size: 1775 32 53
Fragments arranged by size:
1775 53 32
Tth111I GACn'n_nGTC
Cuts at: 0 406 1860
Size: 406 1454
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1635 1860
Size: 1635 225
XmnI GAAnn'nnTTC
Cuts at: 0 1413 1860
Size: 1413 447
Enzymes that do cut:
AcuI AflIII AlwNI ApoI BamHI BanI BbsI BbvCI
BclI BfrBI BpmI Bpu10I BpuEI BsaAI BsaBI BsaXI
BseRI BsmI BspMI BsrBI BsrDI BsrFI BstEII BstXI
BstYI Bsu36I BtgI BtsI EarI EciI Eco57MI Hin4I
HincII HindIII MfeI MmeI MslI NsiI NspI PciI
PflMI PpiI PstI SapI SfcI SmlI SnaBI SspI
StyI TaqII TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BanII BbeI BcgI BciVI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BsaI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmBI Bsp1286I BspEI BspHI BsrGI BssHII BssSI BstAPI
BstBI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI
EaeI EagI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HaeII HpaI KasI KpnI MluI
MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI PacI PasI PfoI PmeI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SpeI SphI SrfI StuI
SwaI XbaI XhoI XmaI ZraI