Restriction map for
pF1KSDA1379
(Linear) (Six-base) MAPSORT of: KIAA1379.seq Check: 5199 from: 1 to: 1332
KIAA1379.orf
With 182 enzymes: SgfI *
February 28, 2007 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 675 1332
Size: 675 657
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 425 1332
Size: 425 907
AlwNI CAG_nnn'CTG
Cuts at: 0 56 1332
Size: 56 1276
BanI G'GyrC_C
Cuts at: 0 30 200 253 761 1129 1268 1332
Size: 30 170 53 508 368 139 64
Fragments arranged by size:
508 368 170 139 64 53 30
BanII G_rGCy'C
Cuts at: 0 1211 1332
Size: 1211 121
BbeI G_GCGC'C
Cuts at: 0 34 204 1133 1332
Size: 34 170 929 199
Fragments arranged by size:
929 199 170 34
BbsI GAAGACnn'nnnn_
Cuts at: 0 616 720 851 1332
Size: 616 104 131 481
Fragments arranged by size:
616 481 131 104
BglI GCCn_nnn'nGGC
Cuts at: 0 28 472 997 1332
Size: 28 444 525 335
Fragments arranged by size:
525 444 335 28
BlpI GC'TnA_GC
Cuts at: 0 1210 1332
Size: 1210 122
Bme1580I G_kGCm'C
Cuts at: 0 1176 1332
Size: 1176 156
BmrI ACTGGGnnnn_n'
Cuts at: 0 195 657 894 1004 1332
Size: 195 462 237 110 328
Fragments arranged by size:
462 328 237 195 110
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1167 1249 1332
Size: 1167 82 83
Fragments arranged by size:
1167 83 82
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 349 478 832 1332
Size: 259 90 129 354 500
Fragments arranged by size:
500 354 259 129 90
Bpu10I CC'TnA_GC
Cuts at: 0 554 1332
Size: 554 778
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 718 1332
Size: 718 614
BsaI GGTCTCn'nnnn_
Cuts at: 0 34 1111 1332
Size: 34 1077 221
Fragments arranged by size:
1077 221 34
BsaAI yAC'GTr
Cuts at: 0 1320 1332
Size: 1320 12
BsaHI Gr'CG_yC
Cuts at: 0 31 201 356 1130 1332
Size: 31 170 155 774 202
Fragments arranged by size:
774 202 170 155 31
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 673 703 1151 1181 1332
Size: 673 30 448 30 151
Fragments arranged by size:
673 448 151 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 52 922 1264 1332
Size: 52 870 342 68
Fragments arranged by size:
870 342 68 52
BseYI C'CCAG_C
Cuts at: 0 1021 1242 1296 1332
Size: 1021 221 54 36
Fragments arranged by size:
1021 221 54 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 274 625 1332
Size: 157 117 351 707
Fragments arranged by size:
707 351 157 117
BsiHKAI G_wGCw'C
Cuts at: 0 1211 1332
Size: 1211 121
BsiWI C'GTAC_G
Cuts at: 0 164 1332
Size: 164 1168
BsmBI CGTCTCn'nnnn_
Cuts at: 0 385 1220 1332
Size: 385 835 112
Fragments arranged by size:
835 385 112
Bsp1286I G_dGCh'C
Cuts at: 0 1176 1211 1332
Size: 1176 35 121
Fragments arranged by size:
1176 121 35
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 675 1332
Size: 675 657
BsrBI CCG'CTC
Cuts at: 0 243 1332
Size: 243 1089
BsrDI GCAATG_nn'
Cuts at: 0 703 1332
Size: 703 629
BssHII G'CGCG_C
Cuts at: 0 144 1332
Size: 144 1188
BstAPI GCAn_nnn'nTGC
Cuts at: 0 603 1332
Size: 603 729
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1320 1332
Size: 1320 12
BtgI C'CryG_G
Cuts at: 0 1029 1272 1332
Size: 1029 243 60
Fragments arranged by size:
1029 243 60
BtsI GCAGTG_nn'
Cuts at: 0 1041 1119 1332
Size: 1041 78 213
Fragments arranged by size:
1041 213 78
ClaI AT'CG_AT
Cuts at: 0 95 1332
Size: 95 1237
DraIII CAC_nnn'GTG
Cuts at: 0 1076 1332
Size: 1076 256
EaeI y'GGCC_r
Cuts at: 0 102 506 1191 1332
Size: 102 404 685 141
Fragments arranged by size:
685 404 141 102
EcoICRI GAG'CTC
Cuts at: 0 1209 1332
Size: 1209 123
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 349 425 478 832 1332
Size: 259 90 76 53 354 500
Fragments arranged by size:
500 354 259 90 76 53
EcoNI CCTnn'n_nnAGG
Cuts at: 0 331 736 1332
Size: 331 405 596
Fragments arranged by size:
596 405 331
EcoO109I rG'GnC_Cy
Cuts at: 0 324 1332
Size: 324 1008
HaeII r_GCGC'y
Cuts at: 0 34 204 982 1133 1332
Size: 34 170 778 151 199
Fragments arranged by size:
778 199 170 151 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 7 39 997 1029 1332
Size: 7 32 958 32 303
Fragments arranged by size:
958 303 32 32 7
KasI G'GCGC_C
Cuts at: 0 30 200 1129 1332
Size: 30 170 929 203
Fragments arranged by size:
929 203 170 30
MscI TGG'CCA
Cuts at: 0 508 1332
Size: 508 824
MslI CAynn'nnrTG
Cuts at: 0 796 886 1332
Size: 796 90 446
Fragments arranged by size:
796 446 90
MspA1I CmG'CkG
Cuts at: 0 1031 1289 1296 1332
Size: 1031 258 7 36
Fragments arranged by size:
1031 258 36 7
NarI GG'CG_CC
Cuts at: 0 31 201 1130 1332
Size: 31 170 929 202
Fragments arranged by size:
929 202 170 31
NspI r_CATG'y
Cuts at: 0 885 1332
Size: 885 447
PflMI CCAn_nnn'nTGG
Cuts at: 0 197 229 669 893 1076 1332
Size: 197 32 440 224 183 256
Fragments arranged by size:
440 256 224 197 183 32
PpuMI rG'GwC_Cy
Cuts at: 0 324 1332
Size: 324 1008
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 772 804 1332
Size: 772 32 528
Fragments arranged by size:
772 528 32
PstI C_TGCA'G
Cuts at: 0 575 1332
Size: 575 757
PvuII CAG'CTG
Cuts at: 0 1296 1332
Size: 1296 36
RsrII CG'GwC_CG
Cuts at: 0 80 1332
Size: 80 1252
SacI G_AGCT'C
Cuts at: 0 1211 1332
Size: 1211 121
SacII CC_GC'GG
Cuts at: 0 1032 1332
Size: 1032 300
SfcI C'TryA_G
Cuts at: 0 571 1332
Size: 571 761
SfoI GGC'GCC
Cuts at: 0 32 202 1131 1332
Size: 32 170 929 201
Fragments arranged by size:
929 201 170 32
SmlI C'TyrA_G
Cuts at: 0 733 1332
Size: 733 599
SphI G_CATG'C
Cuts at: 0 885 1332
Size: 885 447
StuI AGG'CCT
Cuts at: 0 22 475 1332
Size: 22 453 857
Fragments arranged by size:
857 453 22
StyI C'CwwG_G
Cuts at: 0 433 1154 1332
Size: 433 721 178
Fragments arranged by size:
721 433 178
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 367 534 1332
Size: 367 167 798
Fragments arranged by size:
798 367 167
TatI w'GTAC_w
Cuts at: 0 665 1332
Size: 665 667
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1151 1183 1332
Size: 1151 32 149
Fragments arranged by size:
1151 149 32
Enzymes that do cut:
AarI AcuI AlwNI BanI BanII BbeI BbsI BglI
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsiWI
BsmBI Bsp1286I BspMI BsrBI BsrDI BssHII BstAPI BstXI
BtgI BtsI ClaI DraIII EaeI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I KasI MscI MslI MspA1I NarI
NspI PflMI PpuMI PsrI PstI PvuII RsrII SacI
SacII SfcI SfoI SmlI SphI StuI StyI TaqII
TatI TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI
BciVI BclI BfrBI BglII BmgBI BmtI BsaBI BsaWI
BsiEI BsmI BspEI BspHI BsrFI BsrGI BssSI BstBI
BstEII BstYI BstZ17I Bsu36I BtgZI CspCI DraI DrdI
EagI EarI EciI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PvuI SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI SwaI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI