Restriction map for
pF1KSDA1383
(Linear) (Six-base) MAPSORT of: KIAA1383.seq Check: 5630 from: 1 to: 2715
KIAA1383.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1355 1372 1694 2113 2150 2715
Size: 1355 17 322 419 37 565
Fragments arranged by size:
1355 565 419 322 37 17
AflIII A'CryG_T
Cuts at: 0 814 1141 2715
Size: 814 327 1574
Fragments arranged by size:
1574 814 327
AleI CACnn'nnGTG
Cuts at: 0 2242 2715
Size: 2242 473
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2573 2605 2715
Size: 2573 32 110
Fragments arranged by size:
2573 110 32
AlwNI CAG_nnn'CTG
Cuts at: 0 393 651 826 2715
Size: 393 258 175 1889
Fragments arranged by size:
1889 393 258 175
ApoI r'AATT_y
Cuts at: 0 1603 1610 1866 2329 2715
Size: 1603 7 256 463 386
Fragments arranged by size:
1603 463 386 256 7
AseI AT'TA_AT
Cuts at: 0 1166 2715
Size: 1166 1549
BanI G'GyrC_C
Cuts at: 0 368 1450 2715
Size: 368 1082 1265
Fragments arranged by size:
1265 1082 368
BanII G_rGCy'C
Cuts at: 0 1757 2478 2715
Size: 1757 721 237
Fragments arranged by size:
1757 721 237
BbeI G_GCGC'C
Cuts at: 0 372 2715
Size: 372 2343
BbsI GAAGACnn'nnnn_
Cuts at: 0 1378 2715
Size: 1378 1337
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1294 1328 2715
Size: 1294 34 1387
Fragments arranged by size:
1387 1294 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2698 2715
Size: 2698 17
BfrBI ATG'CAT
Cuts at: 0 1699 2715
Size: 1699 1016
BglI GCCn_nnn'nGGC
Cuts at: 0 366 2715
Size: 366 2349
BlpI GC'TnA_GC
Cuts at: 0 565 2715
Size: 565 2150
Bme1580I G_kGCm'C
Cuts at: 0 163 1455 2715
Size: 163 1292 1260
Fragments arranged by size:
1292 1260 163
BmrI ACTGGGnnnn_n'
Cuts at: 0 60 524 2715
Size: 60 464 2191
Fragments arranged by size:
2191 464 60
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 2715
Size: 55 2660
Bpu10I CC'TnA_GC
Cuts at: 0 2284 2715
Size: 2284 431
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 1710 2462 2715
Size: 595 1115 752 253
Fragments arranged by size:
1115 752 595 253
BsaI GGTCTCn'nnnn_
Cuts at: 0 877 2715
Size: 877 1838
BsaHI Gr'CG_yC
Cuts at: 0 369 376 2715
Size: 369 7 2339
Fragments arranged by size:
2339 369 7
BsaWI w'CCGG_w
Cuts at: 0 454 2715
Size: 454 2261
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 127 157 602 632 1901 1931 2715
Size: 127 30 445 30 1269 30 784
Fragments arranged by size:
1269 784 445 127 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 121 130 620 623 893 2715
Size: 121 9 490 3 270 1822
Fragments arranged by size:
1822 490 270 121 9 3
BseYI C'CCAG_C
Cuts at: 0 632 699 1035 1756 2715
Size: 632 67 336 721 959
Fragments arranged by size:
959 721 632 336 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 932 2715
Size: 295 637 1783
Fragments arranged by size:
1783 637 295
BsiEI CG_ry'CG
Cuts at: 0 270 449 2715
Size: 270 179 2266
Fragments arranged by size:
2266 270 179
BsiHKAI G_wGCw'C
Cuts at: 0 2478 2715
Size: 2478 237
BsmI GAATG_Cn'
Cuts at: 0 987 2715
Size: 987 1728
Bsp1286I G_dGCh'C
Cuts at: 0 163 1455 1757 2478 2715
Size: 163 1292 302 721 237
Fragments arranged by size:
1292 721 302 237 163
BspEI T'CCGG_A
Cuts at: 0 454 2715
Size: 454 2261
BspHI T'CATG_A
Cuts at: 0 2175 2715
Size: 2175 540
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2570 2715
Size: 2570 145
BsrBI CCG'CTC
Cuts at: 0 21 510 579 613 2715
Size: 21 489 69 34 2102
Fragments arranged by size:
2102 489 69 34 21
BsrDI GCAATG_nn'
Cuts at: 0 521 837 2634 2668 2715
Size: 521 316 1797 34 47
Fragments arranged by size:
1797 521 316 47 34
BsrFI r'CCGG_y
Cuts at: 0 317 444 1328 2715
Size: 317 127 884 1387
Fragments arranged by size:
1387 884 317 127
BsrGI T'GTAC_A
Cuts at: 0 2313 2715
Size: 2313 402
BstAPI GCAn_nnn'nTGC
Cuts at: 0 281 2715
Size: 281 2434
BstEII G'GTnAC_C
Cuts at: 0 2306 2715
Size: 2306 409
BstXI CCAn_nnnn'nTGG
Cuts at: 0 201 2715
Size: 201 2514
Bsu36I CC'TnA_GG
Cuts at: 0 989 2715
Size: 989 1726
BtgI C'CryG_G
Cuts at: 0 167 244 2715
Size: 167 77 2471
Fragments arranged by size:
2471 167 77
BtsI GCAGTG_nn'
Cuts at: 0 101 312 1452 2715
Size: 101 211 1140 1263
Fragments arranged by size:
1263 1140 211 101
DraI TTT'AAA
Cuts at: 0 1520 2715
Size: 1520 1195
EaeI y'GGCC_r
Cuts at: 0 416 446 2715
Size: 416 30 2269
Fragments arranged by size:
2269 416 30
EagI C'GGCC_G
Cuts at: 0 446 2715
Size: 446 2269
EarI CTCTTCn'nnn_
Cuts at: 0 31 107 292 2715
Size: 31 76 185 2423
Fragments arranged by size:
2423 185 76 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 166 280 2715
Size: 166 114 2435
Fragments arranged by size:
2435 166 114
EcoICRI GAG'CTC
Cuts at: 0 2476 2715
Size: 2476 239
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 1355 1372 1694 2113 2150 2715
Size: 55 1300 17 322 419 37 565
Fragments arranged by size:
1300 565 419 322 55 37 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 856 2715
Size: 856 1859
EcoO109I rG'GnC_Cy
Cuts at: 0 398 2715
Size: 398 2317
EcoRI G'AATT_C
Cuts at: 0 1610 2715
Size: 1610 1105
FseI GG_CCGG'CC
Cuts at: 0 448 2715
Size: 448 2267
HaeII r_GCGC'y
Cuts at: 0 230 372 2715
Size: 230 142 2343
Fragments arranged by size:
2343 230 142
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 127 159 602 634 1282 1314 2095 2127
Size: 127 32 443 32 648 32 781 32
Cuts at: 2127 2184 2216 2356 2388 2715
Size: 57 32 140 32 327
Fragments arranged by size:
781 648 443 327 140 127 57 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1968 2715
Size: 1968 747
KasI G'GCGC_C
Cuts at: 0 368 2715
Size: 368 2347
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1974 2488 2715
Size: 1974 514 227
Fragments arranged by size:
1974 514 227
MscI TGG'CCA
Cuts at: 0 418 2715
Size: 418 2297
MslI CAynn'nnrTG
Cuts at: 0 2029 2242 2715
Size: 2029 213 473
Fragments arranged by size:
2029 473 213
MspA1I CmG'CkG
Cuts at: 0 88 357 651 699 2715
Size: 88 269 294 48 2016
Fragments arranged by size:
2016 294 269 88 48
NaeI GCC'GGC
Cuts at: 0 319 446 2715
Size: 319 127 2269
Fragments arranged by size:
2269 319 127
NarI GG'CG_CC
Cuts at: 0 369 2715
Size: 369 2346
NgoMIV G'CCGG_C
Cuts at: 0 317 444 2715
Size: 317 127 2271
Fragments arranged by size:
2271 317 127
NsiI A_TGCA'T
Cuts at: 0 1701 2715
Size: 1701 1014
NspI r_CATG'y
Cuts at: 0 818 1145 1328 2715
Size: 818 327 183 1387
Fragments arranged by size:
1387 818 327 183
PasI CC'CwG_GG
Cuts at: 0 364 2715
Size: 364 2351
PciI A'CATG_T
Cuts at: 0 814 1141 2715
Size: 814 327 1574
Fragments arranged by size:
1574 814 327
PfoI T'CCnGG_A
Cuts at: 0 2591 2700 2715
Size: 2591 109 15
Fragments arranged by size:
2591 109 15
PstI C_TGCA'G
Cuts at: 0 299 356 656 1074 1647 2715
Size: 299 57 300 418 573 1068
Fragments arranged by size:
1068 573 418 300 299 57
PvuII CAG'CTG
Cuts at: 0 357 651 2715
Size: 357 294 2064
Fragments arranged by size:
2064 357 294
SacI G_AGCT'C
Cuts at: 0 2478 2715
Size: 2478 237
SapI GCTCTTCn'nnn_
Cuts at: 0 31 2715
Size: 31 2684
SfcI C'TryA_G
Cuts at: 0 295 352 652 1070 1643 2715
Size: 295 57 300 418 573 1072
Fragments arranged by size:
1072 573 418 300 295 57
SfoI GGC'GCC
Cuts at: 0 370 2715
Size: 370 2345
SmlI C'TyrA_G
Cuts at: 0 574 1725 2477 2715
Size: 574 1151 752 238
Fragments arranged by size:
1151 752 574 238
SspI AAT'ATT
Cuts at: 0 1432 1902 2652 2715
Size: 1432 470 750 63
Fragments arranged by size:
1432 750 470 63
StuI AGG'CCT
Cuts at: 0 139 2715
Size: 139 2576
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 42 256 2715
Size: 42 214 2459
Fragments arranged by size:
2459 214 42
TatI w'GTAC_w
Cuts at: 0 2313 2602 2715
Size: 2313 289 113
Fragments arranged by size:
2313 289 113
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1901 1933 2715
Size: 1901 32 782
Fragments arranged by size:
1901 782 32
Enzymes that do cut:
AcuI AflIII AleI AloI AlwNI ApoI AseI BanI
BanII BbeI BbsI BcgI BciVI BfrBI BglI BlpI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI
BstEII BstXI Bsu36I BtgI BtsI DraI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI
HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NsiI NspI PasI PciI PfoI
PstI PvuII SacI SapI SfcI SfoI SmlI SspI
StuI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BamHI BbvCI BclI BglII BmgBI BmtI BplI
BsaAI BsaBI BsiWI BsmBI BssHII BssSI BstBI BstYI
BstZ17I BtgZI ClaI CspCI DraIII DrdI EcoRV FalI
FspI FspAI HindIII HpaI KpnI MfeI MluI NcoI
NdeI NheI NotI NruI PacI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StyI
SwaI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI