Restriction map for
pF1KA1385
(Linear) (Six-base) MAPSORT of: KIAA1385.seq Check: 6019 from: 1 to: 2208
KIAA1385.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1740 1909 2208
Size: 1740 169 299
Fragments arranged by size:
1740 299 169
AccI GT'mk_AC
Cuts at: 0 2028 2208
Size: 2028 180
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 785 1340 1559 2208
Size: 63 722 555 219 649
Fragments arranged by size:
722 649 555 219 63
AleI CACnn'nnGTG
Cuts at: 0 931 2208
Size: 931 1277
AlwNI CAG_nnn'CTG
Cuts at: 0 1755 2208
Size: 1755 453
ApaI G_GGCC'C
Cuts at: 0 1435 2208
Size: 1435 773
ApoI r'AATT_y
Cuts at: 0 1337 2208
Size: 1337 871
BamHI G'GATC_C
Cuts at: 0 1950 2208
Size: 1950 258
BanII G_rGCy'C
Cuts at: 0 1435 2165 2208
Size: 1435 730 43
Fragments arranged by size:
1435 730 43
BbsI GAAGACnn'nnnn_
Cuts at: 0 101 623 948 1441 2208
Size: 101 522 325 493 767
Fragments arranged by size:
767 522 493 325 101
BbvCI CC'TCA_GC
Cuts at: 0 787 2208
Size: 787 1421
BciVI GTATCCnnnnn_n'
Cuts at: 0 1731 2208
Size: 1731 477
BglII A'GATC_T
Cuts at: 0 120 2208
Size: 120 2088
Bme1580I G_kGCm'C
Cuts at: 0 1435 2067 2208
Size: 1435 632 141
Fragments arranged by size:
1435 632 141
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 272 275 1021 2208
Size: 272 3 746 1187
Fragments arranged by size:
1187 746 272 3
Bpu10I CC'TnA_GC
Cuts at: 0 787 818 2208
Size: 787 31 1390
Fragments arranged by size:
1390 787 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1052 1729 2208
Size: 1052 677 479
Fragments arranged by size:
1052 677 479
BsaAI yAC'GTr
Cuts at: 0 508 2157 2208
Size: 508 1649 51
Fragments arranged by size:
1649 508 51
BsaWI w'CCGG_w
Cuts at: 0 1011 2208
Size: 1011 1197
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 766 796 1227 1257 2208
Size: 766 30 431 30 951
Fragments arranged by size:
951 766 431 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 575 2208
Size: 575 1633
BseYI C'CCAG_C
Cuts at: 0 1104 2208
Size: 1104 1104
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 897 1907 2208
Size: 897 1010 301
Fragments arranged by size:
1010 897 301
BsiEI CG_ry'CG
Cuts at: 0 8 2208
Size: 8 2200
BsiHKAI G_wGCw'C
Cuts at: 0 767 819 1245 2165 2208
Size: 767 52 426 920 43
Fragments arranged by size:
920 767 426 52 43
BsmI GAATG_Cn'
Cuts at: 0 463 2208
Size: 463 1745
Bsp1286I G_dGCh'C
Cuts at: 0 767 819 1245 1435 2067 2165 2208
Size: 767 52 426 190 632 98 43
Fragments arranged by size:
767 632 426 190 98 52 43
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 437 1740 1909 2208
Size: 437 1303 169 299
Fragments arranged by size:
1303 437 299 169
BsrDI GCAATG_nn'
Cuts at: 0 347 716 2208
Size: 347 369 1492
Fragments arranged by size:
1492 369 347
BssSI C'ACGA_G
Cuts at: 0 275 749 770 812 2208
Size: 275 474 21 42 1396
Fragments arranged by size:
1396 474 275 42 21
BstAPI GCAn_nnn'nTGC
Cuts at: 0 718 2208
Size: 718 1490
BstEII G'GTnAC_C
Cuts at: 0 1612 1896 2208
Size: 1612 284 312
Fragments arranged by size:
1612 312 284
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2060 2208
Size: 2060 148
BstYI r'GATC_y
Cuts at: 0 120 132 448 1778 1950 2208
Size: 120 12 316 1330 172 258
Fragments arranged by size:
1330 316 258 172 120 12
BstZ17I GTA'TAC
Cuts at: 0 2029 2208
Size: 2029 179
BtgI C'CryG_G
Cuts at: 0 1724 2208
Size: 1724 484
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 931 2208
Size: 931 1277
BtsI GCAGTG_nn'
Cuts at: 0 108 2118 2208
Size: 108 2010 90
Fragments arranged by size:
2010 108 90
EaeI y'GGCC_r
Cuts at: 0 1354 1380 1412 1957 2208
Size: 1354 26 32 545 251
Fragments arranged by size:
1354 545 251 32 26
EarI CTCTTCn'nnn_
Cuts at: 0 635 644 1592 2208
Size: 635 9 948 616
Fragments arranged by size:
948 635 616 9
EcoICRI GAG'CTC
Cuts at: 0 2163 2208
Size: 2163 45
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 272 275 785 1021 1340 1559 2208
Size: 63 209 3 510 236 319 219 649
Fragments arranged by size:
649 510 319 236 219 209 63 3
EcoO109I rG'GnC_Cy
Cuts at: 0 1432 2208
Size: 1432 776
EcoRI G'AATT_C
Cuts at: 0 1337 2208
Size: 1337 871
EcoRV GAT'ATC
Cuts at: 0 924 1368 2208
Size: 924 444 840
Fragments arranged by size:
924 840 444
FspI TGC'GCA
Cuts at: 0 2110 2208
Size: 2110 98
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 676 708 766 798 2079 2111 2121 2153
Size: 676 32 58 32 1281 32 10 32
Cuts at: 2153 2208
Size: 55
Fragments arranged by size:
1281 676 58 55 32 32 32 32
10
HincII GTy'rAC
Cuts at: 0 1514 2208
Size: 1514 694
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 30 2208
Size: 30 2178
MscI TGG'CCA
Cuts at: 0 1414 2208
Size: 1414 794
MslI CAynn'nnrTG
Cuts at: 0 931 2208
Size: 931 1277
NcoI C'CATG_G
Cuts at: 0 1724 2208
Size: 1724 484
NspI r_CATG'y
Cuts at: 0 381 954 2109 2208
Size: 381 573 1155 99
Fragments arranged by size:
1155 573 381 99
PfoI T'CCnGG_A
Cuts at: 0 998 2208
Size: 998 1210
PspOMI G'GGCC_C
Cuts at: 0 1431 2208
Size: 1431 777
PstI C_TGCA'G
Cuts at: 0 711 2208
Size: 711 1497
SacI G_AGCT'C
Cuts at: 0 2165 2208
Size: 2165 43
SfcI C'TryA_G
Cuts at: 0 707 839 1460 2208
Size: 707 132 621 748
Fragments arranged by size:
748 707 621 132
SmlI C'TyrA_G
Cuts at: 0 1067 1744 2208
Size: 1067 677 464
Fragments arranged by size:
1067 677 464
SphI G_CATG'C
Cuts at: 0 381 2109 2208
Size: 381 1728 99
Fragments arranged by size:
1728 381 99
StuI AGG'CCT
Cuts at: 0 390 2208
Size: 390 1818
StyI C'CwwG_G
Cuts at: 0 929 1724 2059 2208
Size: 929 795 335 149
Fragments arranged by size:
929 795 335 149
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 22 136 2208
Size: 22 114 2072
Fragments arranged by size:
2072 114 22
TatI w'GTAC_w
Cuts at: 0 1272 2071 2208
Size: 1272 799 137
Fragments arranged by size:
1272 799 137
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 848 880 2208
Size: 848 32 1328
Fragments arranged by size:
1328 848 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1626 2208
Size: 1626 582
XmnI GAAnn'nnTTC
Cuts at: 0 461 2208
Size: 461 1747
Enzymes that do cut:
AarI AccI AcuI AleI AlwNI ApaI ApoI BamHI
BanII BbsI BbvCI BciVI BglII Bme1580I BpmI Bpu10I
BpuEI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspMI BsrDI BssSI BstAPI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI EaeI EarI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI Hin4I HincII
MmeI MscI MslI NcoI NspI PfoI PspOMI PstI
SacI SfcI SmlI SphI StuI StyI TaqII TatI
TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BanI BbeI BcgI BclI BfrBI BglI BlpI
BmgBI BmrI BmtI BplI BsaI BsaBI BsaHI BsiWI
BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BstBI
Bsu36I ClaI CspCI DraI DraIII DrdI EagI EciI
EcoNI FalI FseI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI MspA1I NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI SwaI Tth111I XbaI XhoI XmaI
ZraI