Restriction map for
pF1KA1386
(Linear) (Six-base) MAPSORT of: KIAA1386.seq Check: 2125 from: 1 to: 3267
KIAA1386.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AccI GT'mk_AC
Cuts at: 0 702 2346 3267
Size: 702 1644 921
Fragments arranged by size:
1644 921 702
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 647 727 785 1928 2093 2512 3267
Size: 305 342 80 58 1143 165 419 755
Fragments arranged by size:
1143 755 419 342 305 165 80 58
AflIII A'CryG_T
Cuts at: 0 1716 3267
Size: 1716 1551
AleI CACnn'nnGTG
Cuts at: 0 2437 3267
Size: 2437 830
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 470 1161 1618 3267
Size: 470 691 457 1649
Fragments arranged by size:
1649 691 470 457
AlwNI CAG_nnn'CTG
Cuts at: 0 2368 2535 3267
Size: 2368 167 732
Fragments arranged by size:
2368 732 167
ApaLI G'TGCA_C
Cuts at: 0 229 3267
Size: 229 3038
ApoI r'AATT_y
Cuts at: 0 19 1100 2033 2167 2293 3267
Size: 19 1081 933 134 126 974
Fragments arranged by size:
1081 974 933 134 126 19
AvaI C'yCGr_G
Cuts at: 0 619 2186 2717 3267
Size: 619 1567 531 550
Fragments arranged by size:
1567 619 550 531
BamHI G'GATC_C
Cuts at: 0 615 3267
Size: 615 2652
BanII G_rGCy'C
Cuts at: 0 128 294 783 1359 1896 2815 3267
Size: 128 166 489 576 537 919 452
Fragments arranged by size:
919 576 537 489 452 166 128
BbsI GAAGACnn'nnnn_
Cuts at: 0 1037 2081 2558 3267
Size: 1037 1044 477 709
Fragments arranged by size:
1044 1037 709 477
BbvCI CC'TCA_GC
Cuts at: 0 83 3267
Size: 83 3184
BfrBI ATG'CAT
Cuts at: 0 249 3267
Size: 249 3018
Bme1580I G_kGCm'C
Cuts at: 0 233 3267
Size: 233 3034
BmrI ACTGGGnnnn_n'
Cuts at: 0 798 3267
Size: 798 2469
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1910 2650 3267
Size: 1910 740 617
Fragments arranged by size:
1910 740 617
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1256 1697 2345 2951 3267
Size: 1256 441 648 606 316
Fragments arranged by size:
1256 648 606 441 316
Bpu10I CC'TnA_GC
Cuts at: 0 83 2809 3267
Size: 83 2726 458
Fragments arranged by size:
2726 458 83
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1963 3143 3219 3267
Size: 1963 1180 76 48
Fragments arranged by size:
1963 1180 76 48
BsaAI yAC'GTr
Cuts at: 0 1719 3267
Size: 1719 1548
BsaBI GATnn'nnATC
Cuts at: 0 209 2271 3267
Size: 209 2062 996
Fragments arranged by size:
2062 996 209
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 518 740 1738 2322 2345 3209 3267
Size: 518 222 998 584 23 864 58
Fragments arranged by size:
998 864 584 518 222 58 23
BseYI C'CCAG_C
Cuts at: 0 1394 2996 3267
Size: 1394 1602 271
Fragments arranged by size:
1602 1394 271
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1032 2452 3008 3267
Size: 1032 1420 556 259
Fragments arranged by size:
1420 1032 556 259
BsiHKAI G_wGCw'C
Cuts at: 0 233 783 1359 1896 1958 2815 3267
Size: 233 550 576 537 62 857 452
Fragments arranged by size:
857 576 550 537 452 233 62
BsmI GAATG_Cn'
Cuts at: 0 1973 3267
Size: 1973 1294
Bsp1286I G_dGCh'C
Cuts at: 0 128 233 294 783 1359 1896 1958 2815
Size: 128 105 61 489 576 537 62 857
Cuts at: 2815 3267
Size: 452
Fragments arranged by size:
857 576 537 489 452 128 105 62
61
BspHI T'CATG_A
Cuts at: 0 783 2765 2931 3267
Size: 783 1982 166 336
Fragments arranged by size:
1982 783 336 166
BsrDI GCAATG_nn'
Cuts at: 0 1345 3267
Size: 1345 1922
BsrGI T'GTAC_A
Cuts at: 0 2148 3267
Size: 2148 1119
BstYI r'GATC_y
Cuts at: 0 615 693 1324 3267
Size: 615 78 631 1943
Fragments arranged by size:
1943 631 615 78
Bsu36I CC'TnA_GG
Cuts at: 0 462 689 2652 3267
Size: 462 227 1963 615
Fragments arranged by size:
1963 615 462 227
BtgI C'CryG_G
Cuts at: 0 2438 3267
Size: 2438 829
BtsI GCAGTG_nn'
Cuts at: 0 1612 2587 3267
Size: 1612 975 680
Fragments arranged by size:
1612 975 680
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1917 1952 3267
Size: 1917 35 1315
Fragments arranged by size:
1917 1315 35
DraI TTT'AAA
Cuts at: 0 1815 3267
Size: 1815 1452
DraIII CAC_nnn'GTG
Cuts at: 0 880 3267
Size: 880 2387
DrdI GACnn_nn'nnGTC
Cuts at: 0 2324 3267
Size: 2324 943
EaeI y'GGCC_r
Cuts at: 0 2877 3267
Size: 2877 390
EarI CTCTTCn'nnn_
Cuts at: 0 330 942 1393 1643 2152 2481 2531 3267
Size: 330 612 451 250 509 329 50 736
Fragments arranged by size:
736 612 509 451 330 329 250 50
EcoICRI GAG'CTC
Cuts at: 0 781 1357 1894 2813 3267
Size: 781 576 537 919 454
Fragments arranged by size:
919 781 576 537 454
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 647 727 785 1256 1697 1928 2093
Size: 305 342 80 58 471 441 231 165
Cuts at: 2093 2345 2512 2951 3267
Size: 252 167 439 316
Fragments arranged by size:
471 441 439 342 316 305 252 231
167 165 80 58
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1271 3267
Size: 1271 1996
EcoO109I rG'GnC_Cy
Cuts at: 0 265 2883 3267
Size: 265 2618 384
Fragments arranged by size:
2618 384 265
EcoRI G'AATT_C
Cuts at: 0 2167 3267
Size: 2167 1100
EcoRV GAT'ATC
Cuts at: 0 281 1170 3267
Size: 281 889 2097
Fragments arranged by size:
2097 889 281
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 115 310 733 790 1644 3267
Size: 115 195 423 57 854 1623
Fragments arranged by size:
1623 854 423 195 115 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1714 1746 2854 2886 3267
Size: 1714 32 1108 32 381
Fragments arranged by size:
1714 1108 381 32 32
HindIII A'AGCT_T
Cuts at: 0 767 812 3267
Size: 767 45 2455
Fragments arranged by size:
2455 767 45
MfeI C'AATT_G
Cuts at: 0 1521 1882 3267
Size: 1521 361 1385
Fragments arranged by size:
1521 1385 361
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 848 1176 3267
Size: 848 328 2091
Fragments arranged by size:
2091 848 328
MscI TGG'CCA
Cuts at: 0 2879 3267
Size: 2879 388
MslI CAynn'nnrTG
Cuts at: 0 1006 2437 3267
Size: 1006 1431 830
Fragments arranged by size:
1431 1006 830
MspA1I CmG'CkG
Cuts at: 0 2875 3267
Size: 2875 392
NdeI CA'TA_TG
Cuts at: 0 672 1140 1995 3267
Size: 672 468 855 1272
Fragments arranged by size:
1272 855 672 468
NsiI A_TGCA'T
Cuts at: 0 251 3267
Size: 251 3016
NspI r_CATG'y
Cuts at: 0 741 1605 2144 3267
Size: 741 864 539 1123
Fragments arranged by size:
1123 864 741 539
PflMI CCAn_nnn'nTGG
Cuts at: 0 880 1259 3267
Size: 880 379 2008
Fragments arranged by size:
2008 880 379
PmlI CAC'GTG
Cuts at: 0 1719 3267
Size: 1719 1548
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2321 2353 3267
Size: 2321 32 914
Fragments arranged by size:
2321 914 32
PpuMI rG'GwC_Cy
Cuts at: 0 265 2883 3267
Size: 265 2618 384
Fragments arranged by size:
2618 384 265
PstI C_TGCA'G
Cuts at: 0 353 735 2205 3267
Size: 353 382 1470 1062
Fragments arranged by size:
1470 1062 382 353
PvuII CAG'CTG
Cuts at: 0 2875 3267
Size: 2875 392
SacI G_AGCT'C
Cuts at: 0 783 1359 1896 2815 3267
Size: 783 576 537 919 452
Fragments arranged by size:
919 783 576 537 452
SapI GCTCTTCn'nnn_
Cuts at: 0 330 942 2481 3267
Size: 330 612 1539 786
Fragments arranged by size:
1539 786 612 330
SfcI C'TryA_G
Cuts at: 0 349 731 1213 2201 2975 3267
Size: 349 382 482 988 774 292
Fragments arranged by size:
988 774 482 382 349 292
SmlI C'TyrA_G
Cuts at: 0 1978 3158 3234 3267
Size: 1978 1180 76 33
Fragments arranged by size:
1978 1180 76 33
SphI G_CATG'C
Cuts at: 0 2144 3267
Size: 2144 1123
SspI AAT'ATT
Cuts at: 0 182 2260 3135 3267
Size: 182 2078 875 132
Fragments arranged by size:
2078 875 182 132
StuI AGG'CCT
Cuts at: 0 687 1231 2100 2427 3267
Size: 687 544 869 327 840
Fragments arranged by size:
869 840 687 544 327
StyI C'CwwG_G
Cuts at: 0 922 1404 1733 2221 2543 3267
Size: 922 482 329 488 322 724
Fragments arranged by size:
922 724 488 482 329 322
TatI w'GTAC_w
Cuts at: 0 1693 2148 3255 3267
Size: 1693 455 1107 12
Fragments arranged by size:
1693 1107 455 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1325 1357 2461 2493 3267
Size: 1325 32 1104 32 774
Fragments arranged by size:
1325 1104 774 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2321 2692 3267
Size: 2321 371 575
Fragments arranged by size:
2321 575 371
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1872 3267
Size: 1872 1395
XmnI GAAnn'nnTTC
Cuts at: 0 1709 3134 3267
Size: 1709 1425 133
Fragments arranged by size:
1709 1425 133
Enzymes that do cut:
AccI AcuI AflIII AleI AlfI AlwNI ApaLI ApoI
AvaI BamHI BanII BbsI BbvCI BfrBI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaAI BsaBI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrGI BstYI
Bsu36I BtgI BtsI CspCI DraI DraIII DrdI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
Hin4I HindIII MfeI MmeI MscI MslI MspA1I NdeI
NsiI NspI PflMI PmlI PpiI PpuMI PstI PvuII
SacI SapI SfcI SmlI SphI SspI StuI StyI
TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaI AscI AseI AsiSI AvrII BaeI BanI
BbeI BcgI BciVI BclI BglI BglII BlpI BmgBI
BmtI BsaI BsaHI BsaWI BsaXI BsiEI BsiWI BsmBI
BspEI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I BtgZI ClaI EagI EciI FseI
FspI FspAI HaeII HincII HpaI KasI KpnI MluI
NaeI NarI NcoI NgoMIV NheI NotI NruI PacI
PasI PciI PfoI PmeI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SwaI TaqII XbaI XhoI XmaI ZraI