Restriction map for
pF1KSDA1391
(Linear) (Six-base) MAPSORT of: KIAA1391.seq Check: 4718 from: 1 to: 3573
KIAA1391.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 946 1176 1659 2248 3314 3573
Size: 523 423 230 483 589 1066 259
Fragments arranged by size:
1066 589 523 483 423 259 230
AfeI AGC'GCT
Cuts at: 0 138 3573
Size: 138 3435
AflII C'TTAA_G
Cuts at: 0 3282 3397 3573
Size: 3282 115 176
Fragments arranged by size:
3282 176 115
AflIII A'CryG_T
Cuts at: 0 680 2263 3573
Size: 680 1583 1310
Fragments arranged by size:
1583 1310 680
AlwNI CAG_nnn'CTG
Cuts at: 0 957 2293 3573
Size: 957 1336 1280
Fragments arranged by size:
1336 1280 957
ApaI G_GGCC'C
Cuts at: 0 2028 3573
Size: 2028 1545
ApaLI G'TGCA_C
Cuts at: 0 2040 3573
Size: 2040 1533
ApoI r'AATT_y
Cuts at: 0 651 1091 1242 1591 2585 3573
Size: 651 440 151 349 994 988
Fragments arranged by size:
994 988 651 440 349 151
AseI AT'TA_AT
Cuts at: 0 2696 3573
Size: 2696 877
AvaI C'yCGr_G
Cuts at: 0 899 1882 3573
Size: 899 983 1691
Fragments arranged by size:
1691 983 899
BanII G_rGCy'C
Cuts at: 0 878 2028 2117 2543 3448 3573
Size: 878 1150 89 426 905 125
Fragments arranged by size:
1150 905 878 426 125 89
BbsI GAAGACnn'nnnn_
Cuts at: 0 2225 2558 2963 3573
Size: 2225 333 405 610
Fragments arranged by size:
2225 610 405 333
BbvCI CC'TCA_GC
Cuts at: 0 3477 3573
Size: 3477 96
BciVI GTATCCnnnnn_n'
Cuts at: 0 2470 3573
Size: 2470 1103
BclI T'GATC_A
Cuts at: 0 1353 3573
Size: 1353 2220
BfrBI ATG'CAT
Cuts at: 0 3081 3573
Size: 3081 492
BglII A'GATC_T
Cuts at: 0 994 1344 3573
Size: 994 350 2229
Fragments arranged by size:
2229 994 350
Bme1580I G_kGCm'C
Cuts at: 0 2028 2044 3573
Size: 2028 16 1529
Fragments arranged by size:
2028 1529 16
BmrI ACTGGGnnnn_n'
Cuts at: 0 1017 1368 3573
Size: 1017 351 2205
Fragments arranged by size:
2205 1017 351
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 155 1731 2931 3573
Size: 155 1576 1200 642
Fragments arranged by size:
1576 1200 642 155
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 491 853 1268 1554 2230 3573
Size: 277 214 362 415 286 676 1343
Fragments arranged by size:
1343 676 415 362 286 277 214
Bpu10I CC'TnA_GC
Cuts at: 0 1906 3477 3573
Size: 1906 1571 96
Fragments arranged by size:
1906 1571 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2491 3573
Size: 2491 1082
BsaI GGTCTCn'nnnn_
Cuts at: 0 1368 2632 3573
Size: 1368 1264 941
Fragments arranged by size:
1368 1264 941
BsaAI yAC'GTr
Cuts at: 0 2367 3573
Size: 2367 1206
BsaWI w'CCGG_w
Cuts at: 0 2995 3573
Size: 2995 578
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1241 1271 2901 2931 3573
Size: 1241 30 1630 30 642
Fragments arranged by size:
1630 1241 642 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 42 144 147 1737 2656 3573
Size: 42 102 3 1590 919 917
Fragments arranged by size:
1590 919 917 102 42 3
BseYI C'CCAG_C
Cuts at: 0 17 931 950 2047 2116 3036 3100 3573
Size: 17 914 19 1097 69 920 64 473
Fragments arranged by size:
1097 920 914 473 69 64 19 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 806 3331 3573
Size: 78 728 2525 242
Fragments arranged by size:
2525 728 242 78
BsiHKAI G_wGCw'C
Cuts at: 0 244 2044 3448 3573
Size: 244 1800 1404 125
Fragments arranged by size:
1800 1404 244 125
BsmI GAATG_Cn'
Cuts at: 0 584 1716 3005 3069 3081 3573
Size: 584 1132 1289 64 12 492
Fragments arranged by size:
1289 1132 584 492 64 12
Bsp1286I G_dGCh'C
Cuts at: 0 244 878 2028 2044 2117 2543 3448 3573
Size: 244 634 1150 16 73 426 905 125
Fragments arranged by size:
1150 905 634 426 244 125 73 16
BspEI T'CCGG_A
Cuts at: 0 2995 3573
Size: 2995 578
BsrBI CCG'CTC
Cuts at: 0 306 3573
Size: 306 3267
BsrDI GCAATG_nn'
Cuts at: 0 2985 3573
Size: 2985 588
BsrFI r'CCGG_y
Cuts at: 0 1974 2099 3573
Size: 1974 125 1474
Fragments arranged by size:
1974 1474 125
BsrGI T'GTAC_A
Cuts at: 0 1991 3573
Size: 1991 1582
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2074 2077 3573
Size: 2074 3 1496
Fragments arranged by size:
2074 1496 3
BstXI CCAn_nnnn'nTGG
Cuts at: 0 939 2406 3573
Size: 939 1467 1167
Fragments arranged by size:
1467 1167 939
BstYI r'GATC_y
Cuts at: 0 994 1344 2000 3573
Size: 994 350 656 1573
Fragments arranged by size:
1573 994 656 350
Bsu36I CC'TnA_GG
Cuts at: 0 1453 1642 2089 2152 3573
Size: 1453 189 447 63 1421
Fragments arranged by size:
1453 1421 447 189 63
BtgI C'CryG_G
Cuts at: 0 3059 3573
Size: 3059 514
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 22 1040 3573
Size: 22 1018 2533
Fragments arranged by size:
2533 1018 22
BtsI GCAGTG_nn'
Cuts at: 0 916 1806 2005 3253 3573
Size: 916 890 199 1248 320
Fragments arranged by size:
1248 916 890 320 199
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 398 433 3573
Size: 398 35 3140
Fragments arranged by size:
3140 398 35
DraI TTT'AAA
Cuts at: 0 984 2271 3573
Size: 984 1287 1302
Fragments arranged by size:
1302 1287 984
DraIII CAC_nnn'GTG
Cuts at: 0 2892 3573
Size: 2892 681
DrdI GACnn_nn'nnGTC
Cuts at: 0 69 3573
Size: 69 3504
EaeI y'GGCC_r
Cuts at: 0 267 341 491 1821 2396 3125 3573
Size: 267 74 150 1330 575 729 448
Fragments arranged by size:
1330 729 575 448 267 150 74
EarI CTCTTCn'nnn_
Cuts at: 0 60 574 746 1385 1678 3098 3176 3453
Size: 60 514 172 639 293 1420 78 277
Cuts at: 3453 3573
Size: 120
Fragments arranged by size:
1420 639 514 293 277 172 120 78
60
EcoICRI GAG'CTC
Cuts at: 0 3446 3573
Size: 3446 127
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 491 523 853 946 1176 1268 1554
Size: 277 214 32 330 93 230 92 286
Cuts at: 1554 1659 2230 2248 3314 3573
Size: 105 571 18 1066 259
Fragments arranged by size:
1066 571 330 286 277 259 230 214
105 93 92 32 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1073 2151 2248 2577 3573
Size: 1073 1078 97 329 996
Fragments arranged by size:
1078 1073 996 329 97
EcoO109I rG'GnC_Cy
Cuts at: 0 1171 2024 2025 3205 3523 3573
Size: 1171 853 1 1180 318 50
Fragments arranged by size:
1180 1171 853 318 50 1
EcoRI G'AATT_C
Cuts at: 0 651 1242 3573
Size: 651 591 2331
Fragments arranged by size:
2331 651 591
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2358 3573
Size: 2358 1215
HaeII r_GCGC'y
Cuts at: 0 51 140 2532 3573
Size: 51 89 2392 1041
Fragments arranged by size:
2392 1041 89 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1258 1290 1646 1678 2901 2933 3573
Size: 1258 32 356 32 1223 32 640
Fragments arranged by size:
1258 1223 640 356 32 32 32
HincII GTy'rAC
Cuts at: 0 210 3573
Size: 210 3363
HindIII A'AGCT_T
Cuts at: 0 2140 2340 3573
Size: 2140 200 1233
Fragments arranged by size:
2140 1233 200
MscI TGG'CCA
Cuts at: 0 343 493 1823 2398 3127 3573
Size: 343 150 1330 575 729 446
Fragments arranged by size:
1330 729 575 446 343 150
MslI CAynn'nnrTG
Cuts at: 0 237 1987 2404 3573
Size: 237 1750 417 1169
Fragments arranged by size:
1750 1169 417 237
MspA1I CmG'CkG
Cuts at: 0 92 2065 2189 2615 3292 3573
Size: 92 1973 124 426 677 281
Fragments arranged by size:
1973 677 426 281 124 92
NcoI C'CATG_G
Cuts at: 0 3059 3573
Size: 3059 514
NdeI CA'TA_TG
Cuts at: 0 789 3573
Size: 789 2784
NsiI A_TGCA'T
Cuts at: 0 3083 3573
Size: 3083 490
NspI r_CATG'y
Cuts at: 0 218 684 2267 3229 3573
Size: 218 466 1583 962 344
Fragments arranged by size:
1583 962 466 344 218
PciI A'CATG_T
Cuts at: 0 680 2263 3573
Size: 680 1583 1310
Fragments arranged by size:
1583 1310 680
PflMI CCAn_nnn'nTGG
Cuts at: 0 772 3065 3107 3488 3573
Size: 772 2293 42 381 85
Fragments arranged by size:
2293 772 381 85 42
PfoI T'CCnGG_A
Cuts at: 0 152 869 1323 2246 3344 3573
Size: 152 717 454 923 1098 229
Fragments arranged by size:
1098 923 717 454 229 152
PmlI CAC'GTG
Cuts at: 0 2367 3573
Size: 2367 1206
PpuMI rG'GwC_Cy
Cuts at: 0 1171 3205 3573
Size: 1171 2034 368
Fragments arranged by size:
2034 1171 368
PspOMI G'GGCC_C
Cuts at: 0 2024 3573
Size: 2024 1549
PstI C_TGCA'G
Cuts at: 0 948 1222 2070 3573
Size: 948 274 848 1503
Fragments arranged by size:
1503 948 848 274
PvuII CAG'CTG
Cuts at: 0 92 2065 2189 2615 3292 3573
Size: 92 1973 124 426 677 281
Fragments arranged by size:
1973 677 426 281 124 92
SacI G_AGCT'C
Cuts at: 0 3448 3573
Size: 3448 125
SapI GCTCTTCn'nnn_
Cuts at: 0 574 3453 3573
Size: 574 2879 120
Fragments arranged by size:
2879 574 120
SfcI C'TryA_G
Cuts at: 0 944 1218 2066 3363 3527 3573
Size: 944 274 848 1297 164 46
Fragments arranged by size:
1297 944 848 274 164 46
SgrAI Cr'CCGG_yG
Cuts at: 0 2099 3573
Size: 2099 1474
SmlI C'TyrA_G
Cuts at: 0 2506 3282 3397 3573
Size: 2506 776 115 176
Fragments arranged by size:
2506 776 176 115
SspI AAT'ATT
Cuts at: 0 1107 1320 3573
Size: 1107 213 2253
Fragments arranged by size:
2253 1107 213
StuI AGG'CCT
Cuts at: 0 290 1895 3573
Size: 290 1605 1678
Fragments arranged by size:
1678 1605 290
StyI C'CwwG_G
Cuts at: 0 605 2515 2791 3059 3463 3573
Size: 605 1910 276 268 404 110
Fragments arranged by size:
1910 605 404 276 268 110
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2061 2916 3573
Size: 2061 855 657
Fragments arranged by size:
2061 855 657
TatI w'GTAC_w
Cuts at: 0 1257 1991 3573
Size: 1257 734 1582
Fragments arranged by size:
1582 1257 734
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2351 2383 3573
Size: 2351 32 1190
Fragments arranged by size:
2351 1190 32
Tth111I GACn'n_nGTC
Cuts at: 0 1846 3573
Size: 1846 1727
XbaI T'CTAG_A
Cuts at: 0 555 3495 3573
Size: 555 2940 78
Fragments arranged by size:
2940 555 78
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1137 1550 3573
Size: 1137 413 2023
Fragments arranged by size:
2023 1137 413
XmnI GAAnn'nnTTC
Cuts at: 0 1943 2374 2589 3573
Size: 1943 431 215 984
Fragments arranged by size:
1943 984 431 215
Enzymes that do cut:
AcuI AfeI AflII AflIII AlwNI ApaI ApaLI ApoI
AseI AvaI BanII BbsI BbvCI BciVI BclI BfrBI
BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspEI BsrBI BsrDI BsrFI BsrGI BstAPI BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI HaeII Hin4I HincII HindIII MscI MslI MspA1I
NcoI NdeI NsiI NspI PciI PflMI PfoI PmlI
PpuMI PspOMI PstI PvuII SacI SapI SfcI SgrAI
SmlI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AgeI AhdI AleI
AlfI AloI AscI AsiSI AvrII BaeI BamHI BanI
BbeI BcgI BglI BlpI BmgBI BmtI BsaBI BsaHI
BsiEI BsiWI BsmBI BspHI BspMI BssHII BssSI BstBI
BstEII BstZ17I ClaI EagI EciI EcoRV FseI FspI
FspAI HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NgoMIV NheI NotI NruI PacI PasI PmeI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SmaI SnaBI SpeI SphI SrfI SwaI XhoI XmaI
ZraI