Restriction map for
pF1KSDA1392
(Linear) (Six-base) MAPSORT of: KIAA1392.seq Check: 1990 from: 1 to: 2778
KIAA1392.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2199 2778
Size: 2199 579
Acc65I G'GTAC_C
Cuts at: 0 470 2778
Size: 470 2308
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 896 1559 2036 2778
Size: 896 663 477 742
Fragments arranged by size:
896 742 663 477
AfeI AGC'GCT
Cuts at: 0 1704 2778
Size: 1704 1074
AgeI A'CCGG_T
Cuts at: 0 497 2778
Size: 497 2281
AhdI GACnn_n'nnGTC
Cuts at: 0 1431 1616 2038 2778
Size: 1431 185 422 740
Fragments arranged by size:
1431 740 422 185
AleI CACnn'nnGTG
Cuts at: 0 1282 2051 2778
Size: 1282 769 727
Fragments arranged by size:
1282 769 727
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 40 2069 2778
Size: 40 2029 709
Fragments arranged by size:
2029 709 40
ApaI G_GGCC'C
Cuts at: 0 1993 2778
Size: 1993 785
ApaLI G'TGCA_C
Cuts at: 0 507 616 1399 2778
Size: 507 109 783 1379
Fragments arranged by size:
1379 783 507 109
ApoI r'AATT_y
Cuts at: 0 341 732 793 2449 2778
Size: 341 391 61 1656 329
Fragments arranged by size:
1656 391 341 329 61
AvaI C'yCGr_G
Cuts at: 0 220 523 920 1288 1735 2416 2588 2778
Size: 220 303 397 368 447 681 172 190
Fragments arranged by size:
681 447 397 368 303 220 190 172
BanI G'GyrC_C
Cuts at: 0 470 699 1215 1836 2053 2398 2778
Size: 470 229 516 621 217 345 380
Fragments arranged by size:
621 516 470 380 345 229 217
BanII G_rGCy'C
Cuts at: 0 1632 1993 2063 2659 2711 2778
Size: 1632 361 70 596 52 67
Fragments arranged by size:
1632 596 361 70 67 52
BbsI GAAGACnn'nnnn_
Cuts at: 0 13 617 1535 2191 2778
Size: 13 604 918 656 587
Fragments arranged by size:
918 656 604 587 13
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 239 273 566 600 2778
Size: 239 34 293 34 2178
Fragments arranged by size:
2178 293 239 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 482 2778
Size: 482 2296
BglI GCCn_nnn'nGGC
Cuts at: 0 1953 2778
Size: 1953 825
BglII A'GATC_T
Cuts at: 0 383 2778
Size: 383 2395
Bme1580I G_kGCm'C
Cuts at: 0 511 620 1220 1403 1958 1993 2778
Size: 511 109 600 183 555 35 785
Fragments arranged by size:
785 600 555 511 183 109 35
BmgBI CAC'GTC
Cuts at: 0 615 2778
Size: 615 2163
BmrI ACTGGGnnnn_n'
Cuts at: 0 858 2778
Size: 858 1920
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1100 2778
Size: 1100 1678
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 1923 2778
Size: 380 1543 855
Fragments arranged by size:
1543 855 380
BsaI GGTCTCn'nnnn_
Cuts at: 0 506 1118 1136 2778
Size: 506 612 18 1642
Fragments arranged by size:
1642 612 506 18
BsaAI yAC'GTr
Cuts at: 0 308 2584 2778
Size: 308 2276 194
Fragments arranged by size:
2276 308 194
BsaBI GATnn'nnATC
Cuts at: 0 937 2778
Size: 937 1841
BsaHI Gr'CG_yC
Cuts at: 0 1522 2034 2778
Size: 1522 512 744
Fragments arranged by size:
1522 744 512
BsaWI w'CCGG_w
Cuts at: 0 497 812 1427 2778
Size: 497 315 615 1351
Fragments arranged by size:
1351 615 497 315
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1044 1926 1978 2021 2158 2386 2389 2404
Size: 1044 882 52 43 137 228 3 15
Cuts at: 2404 2778
Size: 374
Fragments arranged by size:
1044 882 374 228 137 52 43 15
3
BseYI C'CCAG_C
Cuts at: 0 1173 1567 2062 2270 2778
Size: 1173 394 495 208 508
Fragments arranged by size:
1173 508 495 394 208
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 1589 2778
Size: 91 1498 1189
Fragments arranged by size:
1498 1189 91
BsiHKAI G_wGCw'C
Cuts at: 0 19 299 511 620 1289 1403 1832 2778
Size: 19 280 212 109 669 114 429 946
Fragments arranged by size:
946 669 429 280 212 114 109 19
BsmBI CGTCTCn'nnnn_
Cuts at: 0 887 1816 2331 2778
Size: 887 929 515 447
Fragments arranged by size:
929 887 515 447
Bsp1286I G_dGCh'C
Cuts at: 0 19 299 511 620 1220 1289 1403 1632
Size: 19 280 212 109 600 69 114 229
Cuts at: 1632 1832 1958 1993 2063 2659 2711 2778
Size: 200 126 35 70 596 52 67
Fragments arranged by size:
600 596 280 229 212 200 126 114
109 70 69 67 52 35 19
BspHI T'CATG_A
Cuts at: 0 998 2778
Size: 998 1780
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1914 2199 2778
Size: 1914 285 579
Fragments arranged by size:
1914 579 285
BsrBI CCG'CTC
Cuts at: 0 2421 2778
Size: 2421 357
BsrDI GCAATG_nn'
Cuts at: 0 257 568 2200 2778
Size: 257 311 1632 578
Fragments arranged by size:
1632 578 311 257
BsrFI r'CCGG_y
Cuts at: 0 497 2778
Size: 497 2281
BstXI CCAn_nnnn'nTGG
Cuts at: 0 846 1479 2050 2070 2778
Size: 846 633 571 20 708
Fragments arranged by size:
846 708 633 571 20
BstYI r'GATC_y
Cuts at: 0 227 383 1447 1468 2778
Size: 227 156 1064 21 1310
Fragments arranged by size:
1310 1064 227 156 21
Bsu36I CC'TnA_GG
Cuts at: 0 546 2778
Size: 546 2232
BtgI C'CryG_G
Cuts at: 0 122 968 1098 2049 2664 2778
Size: 122 846 130 951 615 114
Fragments arranged by size:
951 846 615 130 122 114
BtsI GCAGTG_nn'
Cuts at: 0 582 597 2413 2778
Size: 582 15 1816 365
Fragments arranged by size:
1816 582 365 15
DraI TTT'AAA
Cuts at: 0 825 2778
Size: 825 1953
DrdI GACnn_nn'nnGTC
Cuts at: 0 1131 2778
Size: 1131 1647
EaeI y'GGCC_r
Cuts at: 0 239 848 2778
Size: 239 609 1930
Fragments arranged by size:
1930 609 239
EarI CTCTTCn'nnn_
Cuts at: 0 872 2123 2778
Size: 872 1251 655
Fragments arranged by size:
1251 872 655
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 896 1559 1923 2036 2778
Size: 380 516 663 364 113 742
Fragments arranged by size:
742 663 516 380 364 113
EcoO109I rG'GnC_Cy
Cuts at: 0 80 1043 1495 1926 1989 1990 2240 2778
Size: 80 963 452 431 63 1 250 538
Fragments arranged by size:
963 538 452 431 250 80 63 1
EcoRV GAT'ATC
Cuts at: 0 1170 2778
Size: 1170 1608
HaeII r_GCGC'y
Cuts at: 0 1706 2778
Size: 1706 1072
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 49 81 738 770 1417 1449 1602 1634
Size: 49 32 657 32 647 32 153 32
Cuts at: 1634 2024 2056 2326 2358 2778
Size: 390 32 270 32 420
Fragments arranged by size:
657 647 420 390 270 153 49 32
32 32 32 32 32
HindIII A'AGCT_T
Cuts at: 0 2759 2778
Size: 2759 19
KpnI G_GTAC'C
Cuts at: 0 474 2778
Size: 474 2304
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 1175 1475 1798 2630 2771 2778
Size: 349 826 300 323 832 141 7
Fragments arranged by size:
832 826 349 323 300 141 7
MscI TGG'CCA
Cuts at: 0 241 850 2778
Size: 241 609 1928
Fragments arranged by size:
1928 609 241
MslI CAynn'nnrTG
Cuts at: 0 184 1282 1314 2051 2557 2778
Size: 184 1098 32 737 506 221
Fragments arranged by size:
1098 737 506 221 184 32
MspA1I CmG'CkG
Cuts at: 0 124 846 1061 1177 1996 2778
Size: 124 722 215 116 819 782
Fragments arranged by size:
819 782 722 215 124 116
NcoI C'CATG_G
Cuts at: 0 1098 2049 2664 2778
Size: 1098 951 615 114
Fragments arranged by size:
1098 951 615 114
NspI r_CATG'y
Cuts at: 0 631 2778
Size: 631 2147
PasI CC'CwG_GG
Cuts at: 0 1220 2235 2778
Size: 1220 1015 543
Fragments arranged by size:
1220 1015 543
PflMI CCAn_nnn'nTGG
Cuts at: 0 1753 2408 2778
Size: 1753 655 370
Fragments arranged by size:
1753 655 370
PfoI T'CCnGG_A
Cuts at: 0 1435 2778
Size: 1435 1343
PmlI CAC'GTG
Cuts at: 0 308 2778
Size: 308 2470
PpuMI rG'GwC_Cy
Cuts at: 0 80 1043 1926 2240 2778
Size: 80 963 883 314 538
Fragments arranged by size:
963 883 538 314 80
PspOMI G'GGCC_C
Cuts at: 0 1989 2778
Size: 1989 789
PspXI vC'TCGA_Gb
Cuts at: 0 1288 2416 2778
Size: 1288 1128 362
Fragments arranged by size:
1288 1128 362
PstI C_TGCA'G
Cuts at: 0 607 1549 2778
Size: 607 942 1229
Fragments arranged by size:
1229 942 607
PvuII CAG'CTG
Cuts at: 0 846 1177 2778
Size: 846 331 1601
Fragments arranged by size:
1601 846 331
RsrII CG'GwC_CG
Cuts at: 0 57 69 2778
Size: 57 12 2709
Fragments arranged by size:
2709 57 12
SacII CC_GC'GG
Cuts at: 0 125 2778
Size: 125 2653
ScaI AGT'ACT
Cuts at: 0 726 1593 2778
Size: 726 867 1185
Fragments arranged by size:
1185 867 726
SfcI C'TryA_G
Cuts at: 0 603 1545 2734 2778
Size: 603 942 1189 44
Fragments arranged by size:
1189 942 603 44
SmaI CCC'GGG
Cuts at: 0 525 2778
Size: 525 2253
SmlI C'TyrA_G
Cuts at: 0 1288 2416 2778
Size: 1288 1128 362
Fragments arranged by size:
1288 1128 362
SphI G_CATG'C
Cuts at: 0 631 2778
Size: 631 2147
StuI AGG'CCT
Cuts at: 0 1602 2778
Size: 1602 1176
StyI C'CwwG_G
Cuts at: 0 656 1098 1460 2049 2664 2778
Size: 656 442 362 589 615 114
Fragments arranged by size:
656 615 589 442 362 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 192 292 648 720 1100 1492 2530 2778
Size: 192 100 356 72 380 392 1038 248
Fragments arranged by size:
1038 392 380 356 248 192 100 72
TatI w'GTAC_w
Cuts at: 0 724 1591 2778
Size: 724 867 1187
Fragments arranged by size:
1187 867 724
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1624 1656 2778
Size: 1624 32 1122
Fragments arranged by size:
1624 1122 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1497 1947 2671 2778
Size: 1497 450 724 107
Fragments arranged by size:
1497 724 450 107
XhoI C'TCGA_G
Cuts at: 0 1288 2416 2778
Size: 1288 1128 362
Fragments arranged by size:
1288 1128 362
XmaI C'CCGG_G
Cuts at: 0 523 2778
Size: 523 2255
Enzymes that do cut:
AarI Acc65I AcuI AfeI AgeI AhdI AleI AlfI
ApaI ApaLI ApoI AvaI BanI BanII BbsI BcgI
BciVI BglI BglII Bme1580I BmgBI BmrI BplI BpmI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BstXI BstYI Bsu36I BtgI BtsI DraI DrdI EaeI
EarI Eco57MI EcoO109I EcoRV HaeII Hin4I HindIII KpnI
MmeI MscI MslI MspA1I NcoI NspI PasI PflMI
PfoI PmlI PpuMI PspOMI PspXI PstI PvuII RsrII
SacII ScaI SfcI SmaI SmlI SphI StuI StyI
TaqII TatI TstI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI AclI AflII AflIII AloI AlwNI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BclI
BfrBI BlpI BmtI Bpu10I BpuEI BsaXI BsiEI BsiWI
BsmI BspEI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I BtgZI ClaI CspCI DraIII EagI EciI EcoICRI
EcoNI EcoRI FalI FseI FspI FspAI HincII HpaI
KasI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PmeI PpiI PshAI
PsiI PsrI PvuI SacI SalI SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI
SspI SwaI Tth111I XbaI XmnI ZraI