Restriction map for
pF1KSDA1394
(Linear) (Six-base) MAPSORT of: KIAA1394.seq Check: 4829 from: 1 to: 2898
KIAA1394.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1062 1257 2077 2663 2898
Size: 1062 195 820 586 235
Fragments arranged by size:
1062 820 586 235 195
AccI GT'mk_AC
Cuts at: 0 239 2898
Size: 239 2659
Acc65I G'GTAC_C
Cuts at: 0 2049 2898
Size: 2049 849
AleI CACnn'nnGTG
Cuts at: 0 2708 2898
Size: 2708 190
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 391 2227 2898
Size: 391 1836 671
Fragments arranged by size:
1836 671 391
AlwNI CAG_nnn'CTG
Cuts at: 0 705 920 1605 2655 2664 2700 2844 2898
Size: 705 215 685 1050 9 36 144 54
Fragments arranged by size:
1050 705 685 215 144 54 36 9
ApaI G_GGCC'C
Cuts at: 0 121 2861 2898
Size: 121 2740 37
Fragments arranged by size:
2740 121 37
ApaLI G'TGCA_C
Cuts at: 0 2041 2898
Size: 2041 857
AvaI C'yCGr_G
Cuts at: 0 73 208 224 408 463 2153 2898
Size: 73 135 16 184 55 1690 745
Fragments arranged by size:
1690 745 184 135 73 55 16
AvrII C'CTAG_G
Cuts at: 0 792 2898
Size: 792 2106
BamHI G'GATC_C
Cuts at: 0 60 202 2522 2898
Size: 60 142 2320 376
Fragments arranged by size:
2320 376 142 60
BanI G'GyrC_C
Cuts at: 0 173 309 453 1199 1367 1542 2049 2236
Size: 173 136 144 746 168 175 507 187
Cuts at: 2236 2361 2748 2898
Size: 125 387 150
Fragments arranged by size:
746 507 387 187 175 173 168 150
144 136 125
BanII G_rGCy'C
Cuts at: 0 97 121 136 361 409 1670 1803 2450
Size: 97 24 15 225 48 1261 133 647
Cuts at: 2450 2534 2771 2861 2898
Size: 84 237 90 37
Fragments arranged by size:
1261 647 237 225 133 97 90 84
48 37 24 15
BbeI G_GCGC'C
Cuts at: 0 177 1371 1546 2898
Size: 177 1194 175 1352
Fragments arranged by size:
1352 1194 177 175
BbsI GAAGACnn'nnnn_
Cuts at: 0 2427 2898
Size: 2427 471
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1620 1654 2898
Size: 1620 34 1244
Fragments arranged by size:
1620 1244 34
BglI GCCn_nnn'nGGC
Cuts at: 0 92 765 1315 1327 1531 2355 2828 2898
Size: 92 673 550 12 204 824 473 70
Fragments arranged by size:
824 673 550 473 204 92 70 12
BlpI GC'TnA_GC
Cuts at: 0 659 1153 1919 2898
Size: 659 494 766 979
Fragments arranged by size:
979 766 659 494
Bme1580I G_kGCm'C
Cuts at: 0 7 121 314 2000 2045 2239 2861 2898
Size: 7 114 193 1686 45 194 622 37
Fragments arranged by size:
1686 622 194 193 114 45 37 7
BmgBI CAC'GTC
Cuts at: 0 183 2250 2898
Size: 183 2067 648
Fragments arranged by size:
2067 648 183
BmrI ACTGGGnnnn_n'
Cuts at: 0 100 1236 1429 1707 1845 2898
Size: 100 1136 193 278 138 1053
Fragments arranged by size:
1136 1053 278 193 138 100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 409 697 1024 1033 1246 1354 1465
Size: 127 282 288 327 9 213 108 111
Cuts at: 1465 1489 1507 1654 2032 2095 2548 2755 2898
Size: 24 18 147 378 63 453 207 143
Fragments arranged by size:
453 378 327 288 282 213 207 147
143 127 111 108 63 24 18 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1795 2434 2898
Size: 1795 639 464
Fragments arranged by size:
1795 639 464
BsaI GGTCTCn'nnnn_
Cuts at: 0 1549 2680 2898
Size: 1549 1131 218
Fragments arranged by size:
1549 1131 218
BsaAI yAC'GTr
Cuts at: 0 2608 2898
Size: 2608 290
BsaHI Gr'CG_yC
Cuts at: 0 174 948 1368 1543 1625 2630 2898
Size: 174 774 420 175 82 1005 268
Fragments arranged by size:
1005 774 420 268 175 174 82
BsaWI w'CCGG_w
Cuts at: 0 285 1754 2898
Size: 285 1469 1144
Fragments arranged by size:
1469 1144 285
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1030 1060 1192 1222 1480 1510 2898
Size: 1030 30 132 30 258 30 1388
Fragments arranged by size:
1388 1030 258 132 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1030 1033 1036 1273 1780 2752 2779 2898
Size: 1030 3 3 237 507 972 27 119
Fragments arranged by size:
1030 972 507 237 119 27 3 3
BseYI C'CCAG_C
Cuts at: 0 364 710 969 1058 1512 1899 1937 2193
Size: 364 346 259 89 454 387 38 256
Cuts at: 2193 2198 2464 2648 2797 2826 2860 2898
Size: 5 266 184 149 29 34 38
Fragments arranged by size:
454 387 364 346 266 259 256 184
149 89 38 38 34 29 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 937 995 1660 1927 2111 2686 2898
Size: 937 58 665 267 184 575 212
Fragments arranged by size:
937 665 575 267 212 184 58
BsiEI CG_ry'CG
Cuts at: 0 1304 1418 1526 1573 2709 2898
Size: 1304 114 108 47 1136 189
Fragments arranged by size:
1304 1136 189 114 108 47
BsiHKAI G_wGCw'C
Cuts at: 0 1754 1856 2045 2138 2246 2450 2534 2613
Size: 1754 102 189 93 108 204 84 79
Cuts at: 2613 2898
Size: 285
Fragments arranged by size:
1754 285 204 189 108 102 93 84
79
BsmBI CGTCTCn'nnnn_
Cuts at: 0 829 2326 2821 2898
Size: 829 1497 495 77
Fragments arranged by size:
1497 829 495 77
Bsp1286I G_dGCh'C
Cuts at: 0 7 97 121 136 314 361 409 1670
Size: 7 90 24 15 178 47 48 1261
Cuts at: 1670 1754 1803 1856 2000 2045 2138 2239 2246
Size: 84 49 53 144 45 93 101 7
Cuts at: 2246 2450 2534 2613 2771 2861 2898
Size: 204 84 79 158 90 37
Fragments arranged by size:
1261 204 178 158 144 101 93 90
90 84 84 79 53 49 48 47
45 37 24 15 7 7
BspEI T'CCGG_A
Cuts at: 0 285 2898
Size: 285 2613
BspHI T'CATG_A
Cuts at: 0 2066 2898
Size: 2066 832
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 646 888 1062 1257 2077 2519 2555 2663
Size: 646 242 174 195 820 442 36 108
Cuts at: 2663 2898
Size: 235
Fragments arranged by size:
820 646 442 242 235 195 174 108
36
BsrDI GCAATG_nn'
Cuts at: 0 2211 2898
Size: 2211 687
BsrFI r'CCGG_y
Cuts at: 0 683 1339 1417 1518 2898
Size: 683 656 78 101 1380
Fragments arranged by size:
1380 683 656 101 78
BssHII G'CGCG_C
Cuts at: 0 1481 1576 1654 2898
Size: 1481 95 78 1244
Fragments arranged by size:
1481 1244 95 78
BssSI C'ACGA_G
Cuts at: 0 1681 2612 2898
Size: 1681 931 286
Fragments arranged by size:
1681 931 286
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1950 2700 2898
Size: 1950 750 198
Fragments arranged by size:
1950 750 198
BstEII G'GTnAC_C
Cuts at: 0 339 2898
Size: 339 2559
BstXI CCAn_nnnn'nTGG
Cuts at: 0 105 718 1914 2358 2898
Size: 105 613 1196 444 540
Fragments arranged by size:
1196 613 540 444 105
BstYI r'GATC_y
Cuts at: 0 60 202 2522 2898
Size: 60 142 2320 376
Fragments arranged by size:
2320 376 142 60
BtgI C'CryG_G
Cuts at: 0 300 958 1179 1507 1959 2709 2898
Size: 300 658 221 328 452 750 189
Fragments arranged by size:
750 658 452 328 300 221 189
BtsI GCAGTG_nn'
Cuts at: 0 983 1147 2099 2784 2898
Size: 983 164 952 685 114
Fragments arranged by size:
983 952 685 164 114
ClaI AT'CG_AT
Cuts at: 0 2852 2898
Size: 2852 46
DraIII CAC_nnn'GTG
Cuts at: 0 1138 1683 2250 2898
Size: 1138 545 567 648
Fragments arranged by size:
1138 648 567 545
EaeI y'GGCC_r
Cuts at: 0 303 597 1100 1301 1316 1415 1523 2616
Size: 303 294 503 201 15 99 108 1093
Cuts at: 2616 2898
Size: 282
Fragments arranged by size:
1093 503 303 294 282 201 108 99
15
EagI C'GGCC_G
Cuts at: 0 1301 1415 1523 2898
Size: 1301 114 108 1375
Fragments arranged by size:
1375 1301 114 108
EarI CTCTTCn'nnn_
Cuts at: 0 107 1921 2898
Size: 107 1814 977
Fragments arranged by size:
1814 977 107
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 349 2809 2898
Size: 349 2460 89
Fragments arranged by size:
2460 349 89
EcoICRI GAG'CTC
Cuts at: 0 2448 2532 2898
Size: 2448 84 366
Fragments arranged by size:
2448 366 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 409 697 1024 1033 1246 1354 1465
Size: 127 282 288 327 9 213 108 111
Cuts at: 1465 1489 1507 1654 2032 2095 2548 2755 2898
Size: 24 18 147 378 63 453 207 143
Fragments arranged by size:
453 378 327 288 282 213 207 147
143 127 111 108 63 24 18 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1036 1255 1588 2836 2898
Size: 1036 219 333 1248 62
Fragments arranged by size:
1248 1036 333 219 62
EcoO109I rG'GnC_Cy
Cuts at: 0 70 102 117 118 522 592 879 2661
Size: 70 32 15 1 404 70 287 1782
Cuts at: 2661 2697 2742 2841 2898
Size: 36 45 99 57
Fragments arranged by size:
1782 404 287 99 70 70 57 45
36 32 15 1
EcoRV GAT'ATC
Cuts at: 0 891 2898
Size: 891 2007
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 298 2898
Size: 298 2600
FseI GG_CCGG'CC
Cuts at: 0 1343 2898
Size: 1343 1555
HaeII r_GCGC'y
Cuts at: 0 177 927 1000 1237 1371 1546 1619 2898
Size: 177 750 73 237 134 175 73 1279
Fragments arranged by size:
1279 750 237 177 175 134 73 73
HincII GTy'rAC
Cuts at: 0 2544 2898
Size: 2544 354
KasI G'GCGC_C
Cuts at: 0 173 1367 1542 2898
Size: 173 1194 175 1356
Fragments arranged by size:
1356 1194 175 173
KpnI G_GTAC'C
Cuts at: 0 2053 2898
Size: 2053 845
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1108 2321 2898
Size: 1108 1213 577
Fragments arranged by size:
1213 1108 577
MscI TGG'CCA
Cuts at: 0 2618 2898
Size: 2618 280
MslI CAynn'nnrTG
Cuts at: 0 1958 2708 2898
Size: 1958 750 190
Fragments arranged by size:
1958 750 190
MspA1I CmG'CkG
Cuts at: 0 302 960 1062 1181 1414 1509 1548 1602
Size: 302 658 102 119 233 95 39 54
Cuts at: 1602 1674 1903 1941 2402 2898
Size: 72 229 38 461 496
Fragments arranged by size:
658 496 461 302 233 229 119 102
95 72 54 39 38
NaeI GCC'GGC
Cuts at: 0 1341 1419 1520 2898
Size: 1341 78 101 1378
Fragments arranged by size:
1378 1341 101 78
NarI GG'CG_CC
Cuts at: 0 174 1368 1543 2898
Size: 174 1194 175 1355
Fragments arranged by size:
1355 1194 175 174
NcoI C'CATG_G
Cuts at: 0 1959 2898
Size: 1959 939
NgoMIV G'CCGG_C
Cuts at: 0 1339 1417 1518 2898
Size: 1339 78 101 1380
Fragments arranged by size:
1380 1339 101 78
NspI r_CATG'y
Cuts at: 0 420 2349 2898
Size: 420 1929 549
Fragments arranged by size:
1929 549 420
PasI CC'CwG_GG
Cuts at: 0 122 517 882 2898
Size: 122 395 365 2016
Fragments arranged by size:
2016 395 365 122
PflMI CCAn_nnn'nTGG
Cuts at: 0 183 2188 2625 2844 2898
Size: 183 2005 437 219 54
Fragments arranged by size:
2005 437 219 183 54
PfoI T'CCnGG_A
Cuts at: 0 533 2143 2525 2898
Size: 533 1610 382 373
Fragments arranged by size:
1610 533 382 373
PmlI CAC'GTG
Cuts at: 0 2608 2898
Size: 2608 290
PpuMI rG'GwC_Cy
Cuts at: 0 70 102 2898
Size: 70 32 2796
Fragments arranged by size:
2796 70 32
PspOMI G'GGCC_C
Cuts at: 0 117 2857 2898
Size: 117 2740 41
Fragments arranged by size:
2740 117 41
PstI C_TGCA'G
Cuts at: 0 260 272 899 1894 2660 2696 2779 2898
Size: 260 12 627 995 766 36 83 119
Fragments arranged by size:
995 766 627 260 119 83 36 12
PvuII CAG'CTG
Cuts at: 0 1062 1602 1674 1903 1941 2898
Size: 1062 540 72 229 38 957
Fragments arranged by size:
1062 957 540 229 72 38
SacI G_AGCT'C
Cuts at: 0 2450 2534 2898
Size: 2450 84 364
Fragments arranged by size:
2450 364 84
SacII CC_GC'GG
Cuts at: 0 303 961 1182 1510 2898
Size: 303 658 221 328 1388
Fragments arranged by size:
1388 658 328 303 221
SanDI GG'GwC_CC
Cuts at: 0 70 2898
Size: 70 2828
SapI GCTCTTCn'nnn_
Cuts at: 0 1921 2898
Size: 1921 977
SbfI CC_TGCA'GG
Cuts at: 0 899 2660 2696 2898
Size: 899 1761 36 202
Fragments arranged by size:
1761 899 202 36
SexAI A'CCwGG_T
Cuts at: 0 2045 2255 2898
Size: 2045 210 643
Fragments arranged by size:
2045 643 210
SfcI C'TryA_G
Cuts at: 0 246 256 268 895 1890 2656 2692 2775
Size: 246 10 12 627 995 766 36 83
Cuts at: 2775 2898
Size: 123
Fragments arranged by size:
995 766 627 246 123 83 36 12
10
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1315 2898
Size: 1315 1583
SfoI GGC'GCC
Cuts at: 0 175 1369 1544 2898
Size: 175 1194 175 1354
Fragments arranged by size:
1354 1194 175 175
SmaI CCC'GGG
Cuts at: 0 226 410 465 2898
Size: 226 184 55 2433
Fragments arranged by size:
2433 226 184 55
SmlI C'TyrA_G
Cuts at: 0 1810 2449 2898
Size: 1810 639 449
Fragments arranged by size:
1810 639 449
SphI G_CATG'C
Cuts at: 0 2349 2898
Size: 2349 549
SrfI GCCC'GGGC
Cuts at: 0 226 2898
Size: 226 2672
StuI AGG'CCT
Cuts at: 0 581 2898
Size: 581 2317
StyI C'CwwG_G
Cuts at: 0 98 497 792 1959 2898
Size: 98 399 295 1167 939
Fragments arranged by size:
1167 939 399 295 98
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 874 961 1951 2086 2195 2488 2898
Size: 874 87 990 135 109 293 410
Fragments arranged by size:
990 874 410 293 135 109 87
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 309 341 1122 1154 1192 1224 1480 1512
Size: 309 32 781 32 38 32 256 32
Cuts at: 1512 1693 1725 2898
Size: 181 32 1173
Fragments arranged by size:
1173 781 309 256 181 38 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 544 2898
Size: 544 2354
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 570 2185 2217 2898
Size: 570 1615 32 681
Fragments arranged by size:
1615 681 570 32
XmaI C'CCGG_G
Cuts at: 0 224 408 463 2898
Size: 224 184 55 2435
Fragments arranged by size:
2435 224 184 55
Enzymes that do cut:
AarI AccI Acc65I AleI AlfI AlwNI ApaI ApaLI
AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI
BglI BlpI Bme1580I BmgBI BmrI BpmI BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI
BssHII BssSI BstAPI BstEII BstXI BstYI BtgI BtsI
ClaI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRV FalI FseI HaeII HincII KasI
KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PflMI PfoI PmlI PpuMI PspOMI
PstI PvuII SacI SacII SanDI SapI SbfI SexAI
SfcI SfiI SfoI SmaI SmlI SphI SrfI StuI
StyI TaqII TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AclI AcuI AfeI AflII AflIII AgeI AhdI
AloI ApoI AscI AseI AsiSI BaeI BbvCI BciVI
BclI BfrBI BglII BmtI BplI Bpu10I BsaBI BsiWI
BsmI BsrBI BsrGI BstBI BstZ17I Bsu36I BtgZI CspCI
DraI DrdI EcoRI FspI FspAI Hin4I HindIII HpaI
MfeI MluI NdeI NheI NotI NruI NsiI PacI
PciI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SalI ScaI SgfI SgrAI SnaBI SpeI SspI
SwaI TatI XbaI XhoI XmnI ZraI