Restriction map for
pF1KA1395
(Linear) (Six-base) MAPSORT of: KIAA1395.seq Check: 8987 from: 1 to: 6141
KIAA1395.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 60 1904 2843 3057 4580 4925 6141
Size: 60 1844 939 214 1523 345 1216
Fragments arranged by size:
1844 1523 1216 939 345 214 60
AatII G_ACGT'C
Cuts at: 0 3849 5393 6141
Size: 3849 1544 748
Fragments arranged by size:
3849 1544 748
AccI GT'mk_AC
Cuts at: 0 1820 5117 6141
Size: 1820 3297 1024
Fragments arranged by size:
3297 1820 1024
Acc65I G'GTAC_C
Cuts at: 0 3622 3979 6141
Size: 3622 357 2162
Fragments arranged by size:
3622 2162 357
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 1021 1662 3024 3395 4270 4542 5785
Size: 164 857 641 1362 371 875 272 1243
Cuts at: 5785 6141
Size: 356
Fragments arranged by size:
1362 1243 875 857 641 371 356 272
164
AfeI AGC'GCT
Cuts at: 0 1221 3811 6141
Size: 1221 2590 2330
Fragments arranged by size:
2590 2330 1221
AflIII A'CryG_T
Cuts at: 0 1655 6141
Size: 1655 4486
AgeI A'CCGG_T
Cuts at: 0 1812 6141
Size: 1812 4329
AhdI GACnn_n'nnGTC
Cuts at: 0 486 3012 4992 6141
Size: 486 2526 1980 1149
Fragments arranged by size:
2526 1980 1149 486
AleI CACnn'nnGTG
Cuts at: 0 1813 2137 2188 5282 5470 6141
Size: 1813 324 51 3094 188 671
Fragments arranged by size:
3094 1813 671 324 188 51
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3560 6141
Size: 3560 2581
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 892 924 4336 4368 4648 4680 4936 4968
Size: 892 32 3412 32 280 32 256 32
Cuts at: 4968 5224 5256 6141
Size: 256 32 885
Fragments arranged by size:
3412 892 885 280 256 256 32 32
32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 113 199 387 1755 2029 2268 4659 6048
Size: 113 86 188 1368 274 239 2391 1389
Cuts at: 6048 6063 6141
Size: 15 78
Fragments arranged by size:
2391 1389 1368 274 239 188 113 86
78 15
ApaI G_GGCC'C
Cuts at: 0 212 1298 2559 3057 3556 4326 5819 6141
Size: 212 1086 1261 498 499 770 1493 322
Fragments arranged by size:
1493 1261 1086 770 499 498 322 212
ApaLI G'TGCA_C
Cuts at: 0 1195 2149 2191 3415 4861 5176 6141
Size: 1195 954 42 1224 1446 315 965
Fragments arranged by size:
1446 1224 1195 965 954 315 42
ApoI r'AATT_y
Cuts at: 0 229 1789 2176 3127 6141
Size: 229 1560 387 951 3014
Fragments arranged by size:
3014 1560 951 387 229
AvaI C'yCGr_G
Cuts at: 0 77 205 266 289 525 1837 3680 4223
Size: 77 128 61 23 236 1312 1843 543
Cuts at: 4223 4757 4900 5599 6141
Size: 534 143 699 542
Fragments arranged by size:
1843 1312 699 543 542 534 236 143
128 77 61 23
BamHI G'GATC_C
Cuts at: 0 293 1973 6141
Size: 293 1680 4168
Fragments arranged by size:
4168 1680 293
BanI G'GyrC_C
Cuts at: 0 559 708 937 1439 1994 3622 3979 5272
Size: 559 149 229 502 555 1628 357 1293
Cuts at: 5272 6078 6141
Size: 806 63
Fragments arranged by size:
1628 1293 806 559 555 502 357 229
149 63
BanII G_rGCy'C
Cuts at: 0 158 206 212 290 532 827 933 1298
Size: 158 48 6 78 242 295 106 365
Cuts at: 1298 1696 2132 2540 2559 2920 3057 3244 3335
Size: 398 436 408 19 361 137 187 91
Cuts at: 3335 3556 3993 4030 4326 4774 5096 5501 5809
Size: 221 437 37 296 448 322 405 308
Cuts at: 5809 5819 6141
Size: 10 322
Fragments arranged by size:
448 437 436 408 405 398 365 361
322 322 308 296 295 242 221 187
158 137 106 91 78 48 37 19
10 6
BbeI G_GCGC'C
Cuts at: 0 1443 1998 5276 6141
Size: 1443 555 3278 865
Fragments arranged by size:
3278 1443 865 555
BbsI GAAGACnn'nnnn_
Cuts at: 0 545 3030 5740 5882 6141
Size: 545 2485 2710 142 259
Fragments arranged by size:
2710 2485 545 259 142
BbvCI CC'TCA_GC
Cuts at: 0 3548 4607 6141
Size: 3548 1059 1534
Fragments arranged by size:
3548 1534 1059
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1161 1195 1603 1637 2887 2921 6141
Size: 1161 34 408 34 1250 34 3220
Fragments arranged by size:
3220 1250 1161 408 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3513 6141
Size: 3513 2628
BglI GCCn_nnn'nGGC
Cuts at: 0 1189 1691 2528 2587 2989 4879 5577 6141
Size: 1189 502 837 59 402 1890 698 564
Fragments arranged by size:
1890 1189 837 698 564 502 402 59
BglII A'GATC_T
Cuts at: 0 872 5342 6141
Size: 872 4470 799
Fragments arranged by size:
4470 872 799
BlpI GC'TnA_GC
Cuts at: 0 200 1391 2242 5626 6141
Size: 200 1191 851 3384 515
Fragments arranged by size:
3384 1191 851 515 200
Bme1580I G_kGCm'C
Cuts at: 0 212 1199 1298 1942 2153 2195 2559 3057
Size: 212 987 99 644 211 42 364 498
Cuts at: 3057 3419 3556 3732 4281 4290 4326 4865 5180
Size: 362 137 176 549 9 36 539 315
Cuts at: 5180 5819 6141
Size: 639 322
Fragments arranged by size:
987 644 639 549 539 498 364 362
322 315 212 211 176 137 99 42
36 9
BmgBI CAC'GTC
Cuts at: 0 2196 6141
Size: 2196 3945
BmrI ACTGGGnnnn_n'
Cuts at: 0 366 2017 4845 6141
Size: 366 1651 2828 1296
Fragments arranged by size:
2828 1651 1296 366
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 87 497 1618 1969 2221 2476 2546 2767
Size: 87 410 1121 351 252 255 70 221
Cuts at: 2767 2950 3067 3182 3237 4207 5377 5860 6028
Size: 183 117 115 55 970 1170 483 168
Cuts at: 6028 6141
Size: 113
Fragments arranged by size:
1170 1121 970 483 410 351 255 252
221 183 168 117 115 113 87 70
55
Bpu10I CC'TnA_GC
Cuts at: 0 1538 3548 4607 6141
Size: 1538 2010 1059 1534
Fragments arranged by size:
2010 1538 1534 1059
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 787 1471 2212 5485 6141
Size: 787 684 741 3273 656
Fragments arranged by size:
3273 787 741 684 656
BsaAI yAC'GTr
Cuts at: 0 1656 2334 3162 5468 6141
Size: 1656 678 828 2306 673
Fragments arranged by size:
2306 1656 828 678 673
BsaBI GATnn'nnATC
Cuts at: 0 4732 6141
Size: 4732 1409
BsaHI Gr'CG_yC
Cuts at: 0 1325 1440 1995 3846 5273 5390 5698 6141
Size: 1325 115 555 1851 1427 117 308 443
Fragments arranged by size:
1851 1427 1325 555 443 308 117 115
BsaWI w'CCGG_w
Cuts at: 0 1812 2447 4776 6141
Size: 1812 635 2329 1365
Fragments arranged by size:
2329 1812 1365 635
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 892 922 4336 4366 5224 5254 5293 5323
Size: 892 30 3414 30 858 30 39 30
Cuts at: 5323 6141
Size: 818
Fragments arranged by size:
3414 892 858 818 39 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1864 2218 2743 4038 4372 4624 4981 5020
Size: 1864 354 525 1295 334 252 357 39
Cuts at: 5020 5374 5698 6141
Size: 354 324 443
Fragments arranged by size:
1864 1295 525 443 357 354 354 334
324 252 39
BseYI C'CCAG_C
Cuts at: 0 234 1222 1481 1744 2047 2093 2539 3328
Size: 234 988 259 263 303 46 446 789
Cuts at: 3328 3812 3836 3840 4827 5040 5228 6056 6068
Size: 484 24 4 987 213 188 828 12
Cuts at: 6068 6141
Size: 73
Fragments arranged by size:
988 987 828 789 484 446 303 263
259 234 213 188 73 46 24 12
4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 413 1134 1741 2593 2815 3165 4219 4657
Size: 413 721 607 852 222 350 1054 438
Cuts at: 4657 5541 6141
Size: 884 600
Fragments arranged by size:
1054 884 852 721 607 600 438 413
350 222
BsiEI CG_ry'CG
Cuts at: 0 1139 1193 1936 2138 4871 6141
Size: 1139 54 743 202 2733 1270
Fragments arranged by size:
2733 1270 1139 743 202 54
BsiHKAI G_wGCw'C
Cuts at: 0 767 1199 2063 2132 2153 2195 2975 3158
Size: 767 432 864 69 21 42 780 183
Cuts at: 3158 3244 3419 3993 4571 4865 5180 5501 6141
Size: 86 175 574 578 294 315 321 640
Fragments arranged by size:
864 780 767 640 578 574 432 321
315 294 183 175 86 69 42 21
BsiWI C'GTAC_G
Cuts at: 0 2766 6141
Size: 2766 3375
BsmI GAATG_Cn'
Cuts at: 0 277 3382 6141
Size: 277 3105 2759
Fragments arranged by size:
3105 2759 277
BsmBI CGTCTCn'nnnn_
Cuts at: 0 473 5680 6141
Size: 473 5207 461
Fragments arranged by size:
5207 473 461
Bsp1286I G_dGCh'C
Cuts at: 0 158 206 212 290 532 767 827 933
Size: 158 48 6 78 242 235 60 106
Cuts at: 933 1199 1298 1696 1942 2063 2132 2153 2195
Size: 266 99 398 246 121 69 21 42
Cuts at: 2195 2540 2559 2920 2975 3057 3158 3244 3335
Size: 345 19 361 55 82 101 86 91
Cuts at: 3335 3419 3556 3732 3993 4030 4281 4290 4326
Size: 84 137 176 261 37 251 9 36
Cuts at: 4326 4571 4774 4865 5096 5180 5501 5809 5819
Size: 245 203 91 231 84 321 308 10
Cuts at: 5819 6141
Size: 322
Fragments arranged by size:
398 361 345 322 321 308 266 261
251 246 245 242 235 231 203 176
158 137 121 106 101 99 91 91
86 84 84 82 78 69 60 55
48 42 37 36 21 19 10 9
6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 60 1655 1904 2843 3057 3503 4466 4503
Size: 60 1595 249 939 214 446 963 37
Cuts at: 4503 4580 4638 4679 4925 6141
Size: 77 58 41 246 1216
Fragments arranged by size:
1595 1216 963 939 446 249 246 214
77 60 58 41 37
BsrBI CCG'CTC
Cuts at: 0 624 968 6141
Size: 624 344 5173
Fragments arranged by size:
5173 624 344
BsrDI GCAATG_nn'
Cuts at: 0 601 660 2426 3614 3650 6141
Size: 601 59 1766 1188 36 2491
Fragments arranged by size:
2491 1766 1188 601 59 36
BsrFI r'CCGG_y
Cuts at: 0 185 647 677 1262 1577 1758 1812 2005
Size: 185 462 30 585 315 181 54 193
Cuts at: 2005 3795 5071 5351 6141
Size: 1790 1276 280 790
Fragments arranged by size:
1790 1276 790 585 462 315 280 193
185 181 54 30
BsrGI T'GTAC_A
Cuts at: 0 4262 4745 5309 6141
Size: 4262 483 564 832
Fragments arranged by size:
4262 832 564 483
BssHII G'CGCG_C
Cuts at: 0 5580 6141
Size: 5580 561
BssSI C'ACGA_G
Cuts at: 0 2347 2725 3151 3476 4126 4876 6141
Size: 2347 378 426 325 650 750 1265
Fragments arranged by size:
2347 1265 750 650 426 378 325
BstAPI GCAn_nnn'nTGC
Cuts at: 0 113 2268 2838 3143 3423 3727 6020 6048
Size: 113 2155 570 305 280 304 2293 28
Cuts at: 6048 6141
Size: 93
Fragments arranged by size:
2293 2155 570 305 304 280 113 93
28
BstBI TT'CG_AA
Cuts at: 0 3290 6141
Size: 3290 2851
BstEII G'GTnAC_C
Cuts at: 0 3276 4515 5284 5511 6141
Size: 3276 1239 769 227 630
Fragments arranged by size:
3276 1239 769 630 227
BstYI r'GATC_y
Cuts at: 0 293 779 836 872 1973 5342 5867 6141
Size: 293 486 57 36 1101 3369 525 274
Fragments arranged by size:
3369 1101 525 486 293 274 57 36
Bsu36I CC'TnA_GG
Cuts at: 0 214 497 580 1999 3368 4390 6097 6141
Size: 214 283 83 1419 1369 1022 1707 44
Fragments arranged by size:
1707 1419 1369 1022 283 214 83 44
BtgI C'CryG_G
Cuts at: 0 281 332 728 1958 2403 2475 2592 2666
Size: 281 51 396 1230 445 72 117 74
Cuts at: 2666 3529 3644 4922 5084 5171 6141
Size: 863 115 1278 162 87 970
Fragments arranged by size:
1278 1230 970 863 445 396 281 162
117 115 87 74 72 51
BtsI GCAGTG_nn'
Cuts at: 0 93 561 1791 1940 1978 2287 2481 2749
Size: 93 468 1230 149 38 309 194 268
Cuts at: 2749 2766 2903 3499 4250 4281 6141
Size: 17 137 596 751 31 1860
Fragments arranged by size:
1860 1230 751 596 468 309 268 194
149 137 93 38 31 17
ClaI AT'CG_AT
Cuts at: 0 5323 6141
Size: 5323 818
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 4151 4186 6141
Size: 4151 35 1955
Fragments arranged by size:
4151 1955 35
DraIII CAC_nnn'GTG
Cuts at: 0 2334 3162 6141
Size: 2334 828 2979
Fragments arranged by size:
2979 2334 828
EaeI y'GGCC_r
Cuts at: 0 50 187 1136 1190 1202 1264 1933 2135
Size: 50 137 949 54 12 62 669 202
Cuts at: 2135 2297 2382 2708 2844 2966 3071 3432 3482
Size: 162 85 326 136 122 105 361 50
Cuts at: 3482 3641 3647 4310 4485 4808 4868 5168 5711
Size: 159 6 663 175 323 60 300 543
Cuts at: 5711 6141
Size: 430
Fragments arranged by size:
949 669 663 543 430 361 326 323
300 202 175 162 159 137 136 122
105 85 62 60 54 50 50 12
6
EagI C'GGCC_G
Cuts at: 0 1136 1190 1933 2135 4868 6141
Size: 1136 54 743 202 2733 1273
Fragments arranged by size:
2733 1273 1136 743 202 54
EarI CTCTTCn'nnn_
Cuts at: 0 1420 2812 4057 4297 4559 5893 6141
Size: 1420 1392 1245 240 262 1334 248
Fragments arranged by size:
1420 1392 1334 1245 262 248 240
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 4074 4962 6141
Size: 4074 888 1179
Fragments arranged by size:
4074 1179 888
EcoICRI GAG'CTC
Cuts at: 0 2130 3242 3991 5499 6141
Size: 2130 1112 749 1508 642
Fragments arranged by size:
2130 1508 1112 749 642
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 87 164 497 1021 1618 1662 1969 2221
Size: 87 77 333 524 597 44 307 252
Cuts at: 2221 2476 2546 2767 2950 3024 3067 3182 3237
Size: 255 70 221 183 74 43 115 55
Cuts at: 3237 3395 4207 4270 4542 5377 5785 5860 6028
Size: 158 812 63 272 835 408 75 168
Cuts at: 6028 6141
Size: 113
Fragments arranged by size:
835 812 597 524 408 333 307 272
255 252 221 183 168 158 115 113
87 77 75 74 70 63 55 44
43
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2203 4900 6031 6141
Size: 2203 2697 1131 110
Fragments arranged by size:
2697 2203 1131 110
EcoO109I rG'GnC_Cy
Cuts at: 0 132 209 219 441 1294 1295 1410 1740
Size: 132 77 10 222 853 1 115 330
Cuts at: 1740 2160 2366 2555 2556 3552 4322 4323 4656
Size: 420 206 189 1 996 770 1 333
Cuts at: 4656 4719 5766 5815 5833 5967 6141
Size: 63 1047 49 18 134 174
Fragments arranged by size:
1047 996 853 770 420 333 330 222
206 189 174 134 132 115 77 63
49 18 10 1 1 1
EcoRI G'AATT_C
Cuts at: 0 229 3127 6141
Size: 229 2898 3014
Fragments arranged by size:
3014 2898 229
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2892 6141
Size: 2892 3249
FseI GG_CCGG'CC
Cuts at: 0 1581 6141
Size: 1581 4560
FspI TGC'GCA
Cuts at: 0 1702 3121 6141
Size: 1702 1419 3020
Fragments arranged by size:
3020 1702 1419
HaeII r_GCGC'y
Cuts at: 0 18 114 1223 1263 1443 1998 2080 2830
Size: 18 96 1109 40 180 555 82 750
Cuts at: 2830 3802 3813 4448 5276 6063 6141
Size: 972 11 635 828 787 78
Fragments arranged by size:
1109 972 828 787 750 635 555 180
96 82 78 40 18 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 472 504 764 796 828 860 886 918
Size: 472 32 260 32 32 32 26 32
Cuts at: 918 1225 1231 1257 1263 2998 3030 4568 4600
Size: 307 6 26 6 1735 32 1538 32
Cuts at: 4600 4978 5010 6141
Size: 378 32 1131
Fragments arranged by size:
1735 1538 1131 472 378 307 260 32
32 32 32 32 32 32 32 26
26 6 6
HindIII A'AGCT_T
Cuts at: 0 5434 6141
Size: 5434 707
KasI G'GCGC_C
Cuts at: 0 1439 1994 5272 6141
Size: 1439 555 3278 869
Fragments arranged by size:
3278 1439 869 555
KpnI G_GTAC'C
Cuts at: 0 3626 3983 6141
Size: 3626 357 2158
Fragments arranged by size:
3626 2158 357
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1004 3833 4979 5006 6141
Size: 1004 2829 1146 27 1135
Fragments arranged by size:
2829 1146 1135 1004 27
MscI TGG'CCA
Cuts at: 0 1204 2299 2384 2710 2846 3073 3434 3643
Size: 1204 1095 85 326 136 227 361 209
Cuts at: 3643 3649 4312 5713 6141
Size: 6 663 1401 428
Fragments arranged by size:
1401 1204 1095 663 428 361 326 227
209 136 85 6
MslI CAynn'nnrTG
Cuts at: 0 1813 2137 2188 2954 5282 5470 5668 6141
Size: 1813 324 51 766 2328 188 198 473
Fragments arranged by size:
2328 1813 766 473 324 198 188 51
MspA1I CmG'CkG
Cuts at: 0 238 334 426 595 1230 1781 1922 2477
Size: 238 96 92 169 635 551 141 555
Cuts at: 2477 2493 3564 3840 5173 6072 6141
Size: 16 1071 276 1333 899 69
Fragments arranged by size:
1333 1071 899 635 555 551 276 238
169 141 96 92 69 16
NaeI GCC'GGC
Cuts at: 0 187 1264 1579 3797 5073 6141
Size: 187 1077 315 2218 1276 1068
Fragments arranged by size:
2218 1276 1077 1068 315 187
NarI GG'CG_CC
Cuts at: 0 1440 1995 5273 6141
Size: 1440 555 3278 868
Fragments arranged by size:
3278 1440 868 555
NcoI C'CATG_G
Cuts at: 0 3644 5084 6141
Size: 3644 1440 1057
Fragments arranged by size:
3644 1440 1057
NgoMIV G'CCGG_C
Cuts at: 0 185 1262 1577 3795 5071 6141
Size: 185 1077 315 2218 1276 1070
Fragments arranged by size:
2218 1276 1077 1070 315 185
NotI GC'GGCC_GC
Cuts at: 0 4868 6141
Size: 4868 1273
NspI r_CATG'y
Cuts at: 0 1176 2091 3288 5727 6141
Size: 1176 915 1197 2439 414
Fragments arranged by size:
2439 1197 1176 915 414
PasI CC'CwG_GG
Cuts at: 0 126 1571 3696 6141
Size: 126 1445 2125 2445
Fragments arranged by size:
2445 2125 1445 126
PflMI CCAn_nnn'nTGG
Cuts at: 0 2337 2811 4857 6075 6141
Size: 2337 474 2046 1218 66
Fragments arranged by size:
2337 2046 1218 474 66
PfoI T'CCnGG_A
Cuts at: 0 2198 2546 2891 4340 4652 5020 5138 5870
Size: 2198 348 345 1449 312 368 118 732
Cuts at: 5870 6141
Size: 271
Fragments arranged by size:
2198 1449 732 368 348 345 312 271
118
PmlI CAC'GTG
Cuts at: 0 2334 6141
Size: 2334 3807
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 892 924 4336 4368 5224 5256 6141
Size: 892 32 3412 32 856 32 885
Fragments arranged by size:
3412 892 885 856 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 132 219 1410 1740 2160 4656 4719 5766
Size: 132 87 1191 330 420 2496 63 1047
Cuts at: 5766 5833 6141
Size: 67 308
Fragments arranged by size:
2496 1191 1047 420 330 308 132 87
67 63
PspOMI G'GGCC_C
Cuts at: 0 208 1294 2555 3053 3552 4322 5815 6141
Size: 208 1086 1261 498 499 770 1493 326
Fragments arranged by size:
1493 1261 1086 770 499 498 326 208
PspXI vC'TCGA_Gb
Cuts at: 0 4900 6141
Size: 4900 1241
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1636 1668 6141
Size: 1636 32 4473
Fragments arranged by size:
4473 1636 32
PstI C_TGCA'G
Cuts at: 0 118 263 1333 1786 1988 2744 3809 5192
Size: 118 145 1070 453 202 756 1065 1383
Cuts at: 5192 6044 6141
Size: 852 97
Fragments arranged by size:
1383 1070 1065 852 756 453 202 145
118 97
PvuII CAG'CTG
Cuts at: 0 238 595 1230 1781 1922 2493 3840 6072
Size: 238 357 635 551 141 571 1347 2232
Cuts at: 6072 6141
Size: 69
Fragments arranged by size:
2232 1347 635 571 551 357 238 141
69
SacI G_AGCT'C
Cuts at: 0 2132 3244 3993 5501 6141
Size: 2132 1112 749 1508 640
Fragments arranged by size:
2132 1508 1112 749 640
SacII CC_GC'GG
Cuts at: 0 335 2478 5174 6141
Size: 335 2143 2696 967
Fragments arranged by size:
2696 2143 967 335
SanDI GG'GwC_CC
Cuts at: 0 132 5833 6141
Size: 132 5701 308
Fragments arranged by size:
5701 308 132
SapI GCTCTTCn'nnn_
Cuts at: 0 4057 4559 5893 6141
Size: 4057 502 1334 248
Fragments arranged by size:
4057 1334 502 248
SexAI A'CCwGG_T
Cuts at: 0 221 4792 6141
Size: 221 4571 1349
Fragments arranged by size:
4571 1349 221
SfcI C'TryA_G
Cuts at: 0 114 259 738 1329 1638 1782 1984 2740
Size: 114 145 479 591 309 144 202 756
Cuts at: 2740 3805 5188 5788 5810 6040 6141
Size: 1065 1383 600 22 230 101
Fragments arranged by size:
1383 1065 756 600 591 479 309 230
202 145 144 114 101 22
SfoI GGC'GCC
Cuts at: 0 1441 1996 5274 6141
Size: 1441 555 3278 867
Fragments arranged by size:
3278 1441 867 555
SgrAI Cr'CCGG_yG
Cuts at: 0 1812 6141
Size: 1812 4329
SmaI CCC'GGG
Cuts at: 0 79 207 268 291 3682 4225 4759 6141
Size: 79 128 61 23 3391 543 534 1382
Fragments arranged by size:
3391 1382 543 534 128 79 61 23
SmlI C'TyrA_G
Cuts at: 0 802 1486 2227 4900 5500 6141
Size: 802 684 741 2673 600 641
Fragments arranged by size:
2673 802 741 684 641 600
SphI G_CATG'C
Cuts at: 0 1176 2091 6141
Size: 1176 915 4050
Fragments arranged by size:
4050 1176 915
SrfI GCCC'GGGC
Cuts at: 0 207 6141
Size: 207 5934
StuI AGG'CCT
Cuts at: 0 1804 3904 4132 5197 5452 6141
Size: 1804 2100 228 1065 255 689
Fragments arranged by size:
2100 1804 1065 689 255 228
StyI C'CwwG_G
Cuts at: 0 299 1586 2909 3272 3644 4109 4133 5084
Size: 299 1287 1323 363 372 465 24 951
Cuts at: 5084 6141
Size: 1057
Fragments arranged by size:
1323 1287 1057 951 465 372 363 299
24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 955 2085 2736 3765 4330 6096 6141
Size: 955 1130 651 1029 565 1766 45
Fragments arranged by size:
1766 1130 1029 955 651 565 45
TatI w'GTAC_w
Cuts at: 0 174 1724 3887 4262 4745 5309 6141
Size: 174 1550 2163 375 483 564 832
Fragments arranged by size:
2163 1550 832 564 483 375 174
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 5206 5238 6141
Size: 5206 32 903
Fragments arranged by size:
5206 903 32
Tth111I GACn'n_nGTC
Cuts at: 0 358 1466 5389 6141
Size: 358 1108 3923 752
Fragments arranged by size:
3923 1108 752 358
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 957 1593 4116 6141
Size: 957 636 2523 2025
Fragments arranged by size:
2523 2025 957 636
XhoI C'TCGA_G
Cuts at: 0 4900 6141
Size: 4900 1241
XmaI C'CCGG_G
Cuts at: 0 77 205 266 289 3680 4223 4757 6141
Size: 77 128 61 23 3391 543 534 1384
Fragments arranged by size:
3391 1384 543 534 128 77 61 23
XmnI GAAnn'nnTTC
Cuts at: 0 662 3971 4004 5029 6141
Size: 662 3309 33 1025 1112
Fragments arranged by size:
3309 1112 1025 662 33
ZraI GAC'GTC
Cuts at: 0 3847 5391 6141
Size: 3847 1544 750
Fragments arranged by size:
3847 1544 750
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtsI ClaI CspCI
DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HindIII
KasI KpnI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NotI NspI PasI PflMI PfoI PmlI
PpiI PpuMI PspOMI PspXI PsrI PstI PvuII SacI
SacII SanDI SapI SexAI SfcI SfoI SgrAI SmaI
SmlI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AflII AscI AseI AsiSI AvrII BaeI BclI
BfrBI BmtI BplI BsaI BspEI BspHI BstXI BstZ17I
BtgZI DraI DrdI EcoRV FspAI HincII HpaI MfeI
MluI NdeI NheI NruI NsiI PacI PciI PmeI
PshAI PsiI PvuI RsrII SalI SbfI ScaI SfiI
SgfI SnaBI SpeI SspI SwaI XbaI