Restriction map for
pF1KSDA1397
(Linear) (Six-base) MAPSORT of: KIAA1397.seq Check: 1362 from: 1 to: 2034
KIAA1397.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 332 369 558 2034
Size: 332 37 189 1476
Fragments arranged by size:
1476 332 189 37
AatII G_ACGT'C
Cuts at: 0 1184 2034
Size: 1184 850
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 976 2034
Size: 976 1058
AflIII A'CryG_T
Cuts at: 0 511 2034
Size: 511 1523
AleI CACnn'nnGTG
Cuts at: 0 1018 2034
Size: 1018 1016
AlwNI CAG_nnn'CTG
Cuts at: 0 288 546 904 1137 2034
Size: 288 258 358 233 897
Fragments arranged by size:
897 358 288 258 233
ApaLI G'TGCA_C
Cuts at: 0 876 2034
Size: 876 1158
ApoI r'AATT_y
Cuts at: 0 222 1819 1940 2034
Size: 222 1597 121 94
Fragments arranged by size:
1597 222 121 94
AseI AT'TA_AT
Cuts at: 0 1958 2034
Size: 1958 76
AvaI C'yCGr_G
Cuts at: 0 789 1667 2034
Size: 789 878 367
Fragments arranged by size:
878 789 367
BanII G_rGCy'C
Cuts at: 0 278 1685 1729 2034
Size: 278 1407 44 305
Fragments arranged by size:
1407 305 278 44
BbsI GAAGACnn'nnnn_
Cuts at: 0 1769 1962 2034
Size: 1769 193 72
Fragments arranged by size:
1769 193 72
Bme1580I G_kGCm'C
Cuts at: 0 880 1409 2034
Size: 880 529 625
Fragments arranged by size:
880 625 529
BmgBI CAC'GTC
Cuts at: 0 609 1799 2034
Size: 609 1190 235
Fragments arranged by size:
1190 609 235
BmrI ACTGGGnnnn_n'
Cuts at: 0 742 864 1581 2034
Size: 742 122 717 453
Fragments arranged by size:
742 717 453 122
BmtI G_CTAG'C
Cuts at: 0 1610 2034
Size: 1610 424
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1405 1530 1584 1882 2034
Size: 1405 125 54 298 152
Fragments arranged by size:
1405 298 152 125 54
Bpu10I CC'TnA_GC
Cuts at: 0 846 1676 1790 2034
Size: 846 830 114 244
Fragments arranged by size:
846 830 244 114
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 398 2034
Size: 398 1636
BsaAI yAC'GTr
Cuts at: 0 296 395 512 1098 2034
Size: 296 99 117 586 936
Fragments arranged by size:
936 586 296 117 99
BsaHI Gr'CG_yC
Cuts at: 0 1181 2034
Size: 1181 853
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1607 1637 2034
Size: 1607 30 397
Fragments arranged by size:
1607 397 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 170 2034
Size: 170 1864
BseYI C'CCAG_C
Cuts at: 0 904 923 1046 1732 2034
Size: 904 19 123 686 302
Fragments arranged by size:
904 686 302 123 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 126 772 1042 1303 1308 1886 2034
Size: 126 646 270 261 5 578 148
Fragments arranged by size:
646 578 270 261 148 126 5
BsiEI CG_ry'CG
Cuts at: 0 369 560 2034
Size: 369 191 1474
Fragments arranged by size:
1474 369 191
BsiHKAI G_wGCw'C
Cuts at: 0 880 998 1115 1685 2034
Size: 880 118 117 570 349
Fragments arranged by size:
880 570 349 118 117
BsmI GAATG_Cn'
Cuts at: 0 602 2034
Size: 602 1432
BsmBI CGTCTCn'nnnn_
Cuts at: 0 105 2034
Size: 105 1929
Bsp1286I G_dGCh'C
Cuts at: 0 278 880 998 1115 1409 1685 1729 2034
Size: 278 602 118 117 294 276 44 305
Fragments arranged by size:
602 305 294 278 276 118 117 44
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 332 369 558 2034
Size: 332 37 189 1476
Fragments arranged by size:
1476 332 189 37
BsrBI CCG'CTC
Cuts at: 0 845 1314 2034
Size: 845 469 720
Fragments arranged by size:
845 720 469
BsrFI r'CCGG_y
Cuts at: 0 1303 2034
Size: 1303 731
BssSI C'ACGA_G
Cuts at: 0 619 1018 2034
Size: 619 399 1016
Fragments arranged by size:
1016 619 399
BstEII G'GTnAC_C
Cuts at: 0 1051 2034
Size: 1051 983
BstYI r'GATC_y
Cuts at: 0 1263 2034
Size: 1263 771
BtgI C'CryG_G
Cuts at: 0 140 369 588 833 965 1465 1696 1903
Size: 140 229 219 245 132 500 231 207
Cuts at: 1903 1946 2034
Size: 43 88
Fragments arranged by size:
500 245 231 229 219 207 140 132
88 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 314 1137 2034
Size: 314 823 897
Fragments arranged by size:
897 823 314
BtsI GCAGTG_nn'
Cuts at: 0 17 42 1226 1320 2034
Size: 17 25 1184 94 714
Fragments arranged by size:
1184 714 94 25 17
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 969 1004 2034
Size: 969 35 1030
Fragments arranged by size:
1030 969 35
DraIII CAC_nnn'GTG
Cuts at: 0 1119 2034
Size: 1119 915
DrdI GACnn_nn'nnGTC
Cuts at: 0 1180 2034
Size: 1180 854
EaeI y'GGCC_r
Cuts at: 0 137 290 557 1441 2034
Size: 137 153 267 884 593
Fragments arranged by size:
884 593 267 153 137
EagI C'GGCC_G
Cuts at: 0 557 2034
Size: 557 1477
EarI CTCTTCn'nnn_
Cuts at: 0 952 2034
Size: 952 1082
EcoICRI GAG'CTC
Cuts at: 0 1683 2034
Size: 1683 351
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 976 1405 1530 1584 1882 2034
Size: 976 429 125 54 298 152
Fragments arranged by size:
976 429 298 152 125 54
EcoNI CCTnn'n_nnAGG
Cuts at: 0 670 2034
Size: 670 1364
FspI TGC'GCA
Cuts at: 0 1711 2034
Size: 1711 323
HaeII r_GCGC'y
Cuts at: 0 1029 2034
Size: 1029 1005
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 682 714 811 843 1271 1303 1905 1937
Size: 682 32 97 32 428 32 602 32
Cuts at: 1937 2034
Size: 97
Fragments arranged by size:
682 602 428 97 97 32 32 32
32
HincII GTy'rAC
Cuts at: 0 363 2034
Size: 363 1671
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 2034
Size: 68 1966
MscI TGG'CCA
Cuts at: 0 139 292 2034
Size: 139 153 1742
Fragments arranged by size:
1742 153 139
MslI CAynn'nnrTG
Cuts at: 0 319 748 1018 2034
Size: 319 429 270 1016
Fragments arranged by size:
1016 429 319 270
MspA1I CmG'CkG
Cuts at: 0 371 1134 1467 1529 1905 2034
Size: 371 763 333 62 376 129
Fragments arranged by size:
763 376 371 333 129 62
NaeI GCC'GGC
Cuts at: 0 1305 2034
Size: 1305 729
NcoI C'CATG_G
Cuts at: 0 965 1946 2034
Size: 965 981 88
Fragments arranged by size:
981 965 88
NdeI CA'TA_TG
Cuts at: 0 520 2034
Size: 520 1514
NgoMIV G'CCGG_C
Cuts at: 0 1303 2034
Size: 1303 731
NheI G'CTAG_C
Cuts at: 0 1606 2034
Size: 1606 428
NspI r_CATG'y
Cuts at: 0 645 2034
Size: 645 1389
PflMI CCAn_nnn'nTGG
Cuts at: 0 288 1046 2034
Size: 288 758 988
Fragments arranged by size:
988 758 288
PfoI T'CCnGG_A
Cuts at: 0 61 646 2034
Size: 61 585 1388
Fragments arranged by size:
1388 585 61
PmlI CAC'GTG
Cuts at: 0 296 395 2034
Size: 296 99 1639
Fragments arranged by size:
1639 296 99
PshAI GACnn'nnGTC
Cuts at: 0 477 2034
Size: 477 1557
PstI C_TGCA'G
Cuts at: 0 1133 2034
Size: 1133 901
PvuII CAG'CTG
Cuts at: 0 1134 1529 2034
Size: 1134 395 505
Fragments arranged by size:
1134 505 395
SacI G_AGCT'C
Cuts at: 0 1685 2034
Size: 1685 349
SacII CC_GC'GG
Cuts at: 0 372 1468 1906 2034
Size: 372 1096 438 128
Fragments arranged by size:
1096 438 372 128
ScaI AGT'ACT
Cuts at: 0 207 2034
Size: 207 1827
SexAI A'CCwGG_T
Cuts at: 0 2010 2034
Size: 2010 24
SfcI C'TryA_G
Cuts at: 0 1129 2034
Size: 1129 905
SmaI CCC'GGG
Cuts at: 0 1669 2034
Size: 1669 365
SmlI C'TyrA_G
Cuts at: 0 413 2034
Size: 413 1621
StyI C'CwwG_G
Cuts at: 0 965 1627 1946 2034
Size: 965 662 319 88
Fragments arranged by size:
965 662 319 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1632 1671 2034
Size: 1632 39 363
Fragments arranged by size:
1632 363 39
TatI w'GTAC_w
Cuts at: 0 205 2034
Size: 205 1829
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 57 89 466 498 1105 1137 1607 1639
Size: 57 32 377 32 607 32 470 32
Cuts at: 1639 2034
Size: 395
Fragments arranged by size:
607 470 395 377 57 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 631 1285 2034
Size: 631 654 749
Fragments arranged by size:
749 654 631
XmaI C'CCGG_G
Cuts at: 0 1667 2034
Size: 1667 367
ZraI GAC'GTC
Cuts at: 0 1182 2034
Size: 1182 852
Enzymes that do cut:
AarI AatII AcuI AflIII AleI AlwNI ApaLI ApoI
AseI AvaI BanII BbsI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI
BsrFI BssSI BstEII BstYI BtgI BtgZI BtsI CspCI
DraIII DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI
FspI HaeII Hin4I HincII MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NspI PflMI PfoI
PmlI PshAI PstI PvuII SacI SacII ScaI SexAI
SfcI SmaI SmlI StyI TaqII TatI TstI Tth111I
XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AhdI AlfI
AloI ApaI AscI AsiSI AvrII BaeI BamHI BanI
BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI BplI BsaI BsaBI BsaWI BsiWI BspEI BspHI
BsrDI BsrGI BssHII BstAPI BstBI BstXI BstZ17I Bsu36I
ClaI DraI EciI EcoO109I EcoRI EcoRV FalI FseI
FspAI HindIII HpaI KasI KpnI MfeI MluI NarI
NotI NruI NsiI PacI PasI PciI PmeI PpiI
PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII SalI
SanDI SapI SbfI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI SwaI XbaI XcmI
XhoI XmnI