Restriction map for
pF1KA1398
(Linear) (Six-base) MAPSORT of: KIAA1398.seq Check: 3221 from: 1 to: 4680
KIAA1398.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3900 4680
Size: 3900 780
AatII G_ACGT'C
Cuts at: 0 3374 4680
Size: 3374 1306
AccI GT'mk_AC
Cuts at: 0 85 4680
Size: 85 4595
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 656 2782 3760 4339 4454 4516 4591 4680
Size: 656 2126 978 579 115 62 75 89
Fragments arranged by size:
2126 978 656 579 115 89 75 62
AflIII A'CryG_T
Cuts at: 0 4040 4680
Size: 4040 640
AleI CACnn'nnGTG
Cuts at: 0 2719 4680
Size: 2719 1961
AlwNI CAG_nnn'CTG
Cuts at: 0 580 2260 2775 2980 3048 4680
Size: 580 1680 515 205 68 1632
Fragments arranged by size:
1680 1632 580 515 205 68
ApaI G_GGCC'C
Cuts at: 0 795 855 915 945 975 1005 1035 1065
Size: 795 60 60 30 30 30 30 30
Cuts at: 1065 1095 1185 1245 1275 1305 1335 1365 1395
Size: 30 90 60 30 30 30 30 30
Cuts at: 1395 1425 1455 1485 1605 1635 1695 1725 1755
Size: 30 30 30 120 30 60 30 30
Cuts at: 1755 1785 1815 1875 1905 2366 4269 4680
Size: 30 30 60 30 461 1903 411
Fragments arranged by size:
1903 795 461 411 120 90 60 60
60 60 60 30 30 30 30 30
30 30 30 30 30 30 30 30
30 30 30 30 30 30 30
ApoI r'AATT_y
Cuts at: 0 471 4680
Size: 471 4209
AscI GG'CGCG_CC
Cuts at: 0 4542 4680
Size: 4542 138
AvaI C'yCGr_G
Cuts at: 0 2699 3959 4680
Size: 2699 1260 721
Fragments arranged by size:
2699 1260 721
BanI G'GyrC_C
Cuts at: 0 528 543 556 1210 2301 3931 4287 4669
Size: 528 15 13 654 1091 1630 356 382
Cuts at: 4669 4680
Size: 11
Fragments arranged by size:
1630 1091 654 528 382 356 15 13
11
BanII G_rGCy'C
Cuts at: 0 306 645 795 855 885 915 945 975
Size: 306 339 150 60 30 30 30 30
Cuts at: 975 1005 1035 1065 1095 1125 1155 1185 1245
Size: 30 30 30 30 30 30 30 60
Cuts at: 1245 1275 1305 1335 1365 1395 1425 1455 1485
Size: 30 30 30 30 30 30 30 30
Cuts at: 1485 1605 1635 1695 1725 1755 1785 1815 1875
Size: 120 30 60 30 30 30 30 60
Cuts at: 1875 1905 1935 1965 1995 2025 2079 2296 2366
Size: 30 30 30 30 30 54 217 70
Cuts at: 2366 2627 2942 3467 3767 3950 4269 4680
Size: 261 315 525 300 183 319 411
Fragments arranged by size:
525 411 339 319 315 306 300 261
217 183 150 120 70 60 60 60
60 54 30 30 30 30 30 30
30 30 30 30 30 30 30 30
30 30 30 30 30 30 30 30
30 30 30 30 30 30 30
BbeI G_GCGC'C
Cuts at: 0 3935 4291 4680
Size: 3935 356 389
Fragments arranged by size:
3935 389 356
BbsI GAAGACnn'nnnn_
Cuts at: 0 4327 4460 4579 4680
Size: 4327 133 119 101
Fragments arranged by size:
4327 133 119 101
BbvCI CC'TCA_GC
Cuts at: 0 1115 1205 1895 4126 4680
Size: 1115 90 690 2231 554
Fragments arranged by size:
2231 1115 690 554 90
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3391 3425 4183 4217 4680
Size: 3391 34 758 34 463
Fragments arranged by size:
3391 758 463 34 34
BclI T'GATC_A
Cuts at: 0 252 4680
Size: 252 4428
BglI GCCn_nnn'nGGC
Cuts at: 0 880 1000 1030 1060 1090 1150 1240 1270
Size: 880 120 30 30 30 60 90 30
Cuts at: 1270 1330 1360 1390 1420 1450 1480 1600 1630
Size: 60 30 30 30 30 30 120 30
Cuts at: 1630 1690 1720 1750 1780 1810 1930 2671 3169
Size: 60 30 30 30 30 120 741 498
Cuts at: 3169 3385 3535 3556 3652 4680
Size: 216 150 21 96 1028
Fragments arranged by size:
1028 880 741 498 216 150 120 120
120 96 90 60 60 60 30 30
30 30 30 30 30 30 30 30
30 30 30 30 21
BlpI GC'TnA_GC
Cuts at: 0 2941 4680
Size: 2941 1739
Bme1580I G_kGCm'C
Cuts at: 0 548 795 855 915 945 975 1005 1035
Size: 548 247 60 60 30 30 30 30
Cuts at: 1035 1065 1095 1185 1215 1245 1275 1305 1335
Size: 30 30 90 30 30 30 30 30
Cuts at: 1335 1365 1395 1425 1455 1485 1605 1635 1695
Size: 30 30 30 30 30 120 30 60
Cuts at: 1695 1725 1755 1785 1815 1875 1905 2122 2366
Size: 30 30 30 30 60 30 217 244
Cuts at: 2366 4269 4672 4680
Size: 1903 403 8
Fragments arranged by size:
1903 548 403 247 244 217 120 90
60 60 60 60 30 30 30 30
30 30 30 30 30 30 30 30
30 30 30 30 30 30 30 30
30 30 8
BmrI ACTGGGnnnn_n'
Cuts at: 0 325 544 2165 4680
Size: 325 219 1621 2515
Fragments arranged by size:
2515 1621 325 219
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3786 4503 4680
Size: 3786 717 177
Fragments arranged by size:
3786 717 177
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2830 3262 3361 3445 3466 3934 4351 4372
Size: 2830 432 99 84 21 468 417 21
Cuts at: 4372 4680
Size: 308
Fragments arranged by size:
2830 468 432 417 308 99 84 21
21
Bpu10I CC'TnA_GC
Cuts at: 0 1115 1205 1895 2024 3466 4126 4680
Size: 1115 90 690 129 1442 660 554
Fragments arranged by size:
1442 1115 690 660 554 129 90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3400 4680
Size: 3400 1280
BsaI GGTCTCn'nnnn_
Cuts at: 0 2415 3872 4680
Size: 2415 1457 808
Fragments arranged by size:
2415 1457 808
BsaAI yAC'GTr
Cuts at: 0 2757 4242 4680
Size: 2757 1485 438
Fragments arranged by size:
2757 1485 438
BsaBI GATnn'nnATC
Cuts at: 0 257 4680
Size: 257 4423
BsaHI Gr'CG_yC
Cuts at: 0 3061 3371 3932 4288 4680
Size: 3061 310 561 356 392
Fragments arranged by size:
3061 561 392 356 310
BsaWI w'CCGG_w
Cuts at: 0 2789 4680
Size: 2789 1891
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3412 3442 3766 3796 3898 3928 4027 4057
Size: 3412 30 324 30 102 30 99 30
Cuts at: 4057 4680
Size: 623
Fragments arranged by size:
3412 623 324 102 99 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 390 3094 3265 3759 3817 3913 3916 3919
Size: 390 2704 171 494 58 96 3 3
Cuts at: 3919 3955 3958 4411 4495 4680
Size: 36 3 453 84 185
Fragments arranged by size:
2704 494 453 390 185 171 96 84
58 36 3 3 3
BseYI C'CCAG_C
Cuts at: 0 2450 3302 4261 4269 4293 4680
Size: 2450 852 959 8 24 387
Fragments arranged by size:
2450 959 852 387 24 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 2743 2788 3187 3559 4680
Size: 355 2388 45 399 372 1121
Fragments arranged by size:
2388 1121 399 372 355 45
BsiEI CG_ry'CG
Cuts at: 0 3551 4082 4388 4680
Size: 3551 531 306 292
Fragments arranged by size:
3551 531 306 292
BsiHKAI G_wGCw'C
Cuts at: 0 1845 2079 2627 2756 2942 3467 3817 3950
Size: 1845 234 548 129 186 525 350 133
Cuts at: 3950 4040 4680
Size: 90 640
Fragments arranged by size:
1845 640 548 525 350 234 186 133
129 90
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3799 3853 4680
Size: 3799 54 827
Fragments arranged by size:
3799 827 54
Bsp1286I G_dGCh'C
Cuts at: 0 306 548 645 795 855 885 915 945
Size: 306 242 97 150 60 30 30 30
Cuts at: 945 975 1005 1035 1065 1095 1125 1155 1185
Size: 30 30 30 30 30 30 30 30
Cuts at: 1185 1215 1245 1275 1305 1335 1365 1395 1425
Size: 30 30 30 30 30 30 30 30
Cuts at: 1425 1455 1485 1605 1635 1695 1725 1755 1785
Size: 30 30 120 30 60 30 30 30
Cuts at: 1785 1815 1845 1875 1905 1935 1965 1995 2025
Size: 30 30 30 30 30 30 30 30
Cuts at: 2025 2079 2122 2296 2366 2627 2756 2942 3467
Size: 54 43 174 70 261 129 186 525
Cuts at: 3467 3767 3817 3950 4040 4269 4672 4680
Size: 300 50 133 90 229 403 8
Fragments arranged by size:
525 403 306 300 261 242 229 186
174 150 133 129 120 97 90 70
60 60 54 50 43 30 30 30
30 30 30 30 30 30 30 30
30 30 30 30 30 30 30 30
30 30 30 30 30 30 30 30
30 30 30 30 30 30 8
BspEI T'CCGG_A
Cuts at: 0 2789 4680
Size: 2789 1891
BspHI T'CATG_A
Cuts at: 0 255 4680
Size: 255 4425
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3033 3890 3900 4680
Size: 3033 857 10 780
Fragments arranged by size:
3033 857 780 10
BsrFI r'CCGG_y
Cuts at: 0 4453 4680
Size: 4453 227
BssHII G'CGCG_C
Cuts at: 0 4542 4680
Size: 4542 138
BssSI C'ACGA_G
Cuts at: 0 2886 4551 4680
Size: 2886 1665 129
Fragments arranged by size:
2886 1665 129
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2775 3048 3245 4044 4680
Size: 2775 273 197 799 636
Fragments arranged by size:
2775 799 636 273 197
BstBI TT'CG_AA
Cuts at: 0 493 4680
Size: 493 4187
BstEII G'GTnAC_C
Cuts at: 0 2524 4680
Size: 2524 2156
BstXI CCAn_nnnn'nTGG
Cuts at: 0 4116 4680
Size: 4116 564
BstYI r'GATC_y
Cuts at: 0 2465 2786 3711 4680
Size: 2465 321 925 969
Fragments arranged by size:
2465 969 925 321
BtgI C'CryG_G
Cuts at: 0 2414 3267 3530 3545 3638 3758 4680
Size: 2414 853 263 15 93 120 922
Fragments arranged by size:
2414 922 853 263 120 93 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 4208 4680
Size: 4208 472
BtsI GCAGTG_nn'
Cuts at: 0 3817 4680
Size: 3817 863
DraIII CAC_nnn'GTG
Cuts at: 0 3701 4680
Size: 3701 979
EaeI y'GGCC_r
Cuts at: 0 2576 2921 3194 3542 3548 4079 4385 4680
Size: 2576 345 273 348 6 531 306 295
Fragments arranged by size:
2576 531 348 345 306 295 273 6
EagI C'GGCC_G
Cuts at: 0 3548 4079 4385 4680
Size: 3548 531 306 295
Fragments arranged by size:
3548 531 306 295
EarI CTCTTCn'nnn_
Cuts at: 0 221 2552 2801 3883 3977 4228 4680
Size: 221 2331 249 1082 94 251 452
Fragments arranged by size:
2331 1082 452 251 249 221 94
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 619 799 3172 3244 3307 3403 3871 4680
Size: 619 180 2373 72 63 96 468 809
Fragments arranged by size:
2373 809 619 468 180 96 72 63
EcoICRI GAG'CTC
Cuts at: 0 2077 2625 2940 3465 3948 4680
Size: 2077 548 315 525 483 732
Fragments arranged by size:
2077 732 548 525 483 315
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 656 2782 2830 3262 3361 3445 3466 3760
Size: 656 2126 48 432 99 84 21 294
Cuts at: 3760 3934 4339 4351 4372 4454 4516 4591 4680
Size: 174 405 12 21 82 62 75 89
Fragments arranged by size:
2126 656 432 405 294 174 99 89
84 82 75 62 48 21 21 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2845 3103 3340 3586 4680
Size: 2845 258 237 246 1094
Fragments arranged by size:
2845 1094 258 246 237
EcoO109I rG'GnC_Cy
Cuts at: 0 791 851 852 911 941 971 1001 1031
Size: 791 60 1 59 30 30 30 30
Cuts at: 1031 1061 1091 1181 1241 1271 1301 1331 1361
Size: 30 30 90 60 30 30 30 30
Cuts at: 1361 1391 1421 1451 1452 1481 1482 1511 1541
Size: 30 30 30 1 29 1 29 30
Cuts at: 1541 1571 1601 1631 1661 1691 1721 1751 1781
Size: 30 30 30 30 30 30 30 30
Cuts at: 1781 1811 1871 1901 2165 2195 2363 3045 3084
Size: 30 60 30 264 30 168 682 39
Cuts at: 3084 3333 3645 3727 4265 4266 4305 4680
Size: 249 312 82 538 1 39 375
Fragments arranged by size:
791 682 538 375 312 264 249 168
90 82 60 60 60 59 39 39
30 30 30 30 30 30 30 30
30 30 30 30 30 30 30 30
30 30 30 30 30 30 30 30
30 29 29 1 1 1 1
EcoRI G'AATT_C
Cuts at: 0 471 4680
Size: 471 4209
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 4027 4680
Size: 4027 653
FspI TGC'GCA
Cuts at: 0 3238 4680
Size: 3238 1442
HaeII r_GCGC'y
Cuts at: 0 3099 3935 4091 4291 4680
Size: 3099 836 156 200 389
Fragments arranged by size:
3099 836 389 200 156
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 237 269 603 635 2066 2098 2455 2487
Size: 237 32 334 32 1431 32 357 32
Cuts at: 2487 2778 2810 3412 3444 3766 3798 4680
Size: 291 32 602 32 322 32 882
Fragments arranged by size:
1431 882 602 357 334 322 291 237
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 86 4526 4680
Size: 86 4440 154
Fragments arranged by size:
4440 154 86
HindIII A'AGCT_T
Cuts at: 0 2926 4680
Size: 2926 1754
HpaI GTT'AAC
Cuts at: 0 4526 4680
Size: 4526 154
KasI G'GCGC_C
Cuts at: 0 3931 4287 4680
Size: 3931 356 393
Fragments arranged by size:
3931 393 356
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 4680
Size: 349 4331
MscI TGG'CCA
Cuts at: 0 2578 2923 3544 4680
Size: 2578 345 621 1136
Fragments arranged by size:
2578 1136 621 345
MslI CAynn'nnrTG
Cuts at: 0 2719 4680
Size: 2719 1961
MspA1I CmG'CkG
Cuts at: 0 454 2227 2454 2553 2691 2775 2808 2832
Size: 454 1773 227 99 138 84 33 24
Cuts at: 2832 3003 3028 3054 3760 4317 4329 4371 4494
Size: 171 25 26 706 557 12 42 123
Cuts at: 4494 4590 4632 4680
Size: 96 42 48
Fragments arranged by size:
1773 706 557 454 227 171 138 123
99 96 84 48 42 42 33 26
25 24 12
NaeI GCC'GGC
Cuts at: 0 4455 4680
Size: 4455 225
NarI GG'CG_CC
Cuts at: 0 3932 4288 4680
Size: 3932 356 392
Fragments arranged by size:
3932 392 356
NcoI C'CATG_G
Cuts at: 0 3530 3638 4680
Size: 3530 108 1042
Fragments arranged by size:
3530 1042 108
NdeI CA'TA_TG
Cuts at: 0 111 4680
Size: 111 4569
NgoMIV G'CCGG_C
Cuts at: 0 4453 4680
Size: 4453 227
NotI GC'GGCC_GC
Cuts at: 0 4079 4680
Size: 4079 601
NspI r_CATG'y
Cuts at: 0 2736 3873 4418 4680
Size: 2736 1137 545 262
Fragments arranged by size:
2736 1137 545 262
PasI CC'CwG_GG
Cuts at: 0 861 4680
Size: 861 3819
PflMI CCAn_nnn'nTGG
Cuts at: 0 580 4680
Size: 580 4100
PfoI T'CCnGG_A
Cuts at: 0 3278 3509 4349 4680
Size: 3278 231 840 331
Fragments arranged by size:
3278 840 331 231
PmlI CAC'GTG
Cuts at: 0 2757 4680
Size: 2757 1923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3306 3338 4680
Size: 3306 32 1342
Fragments arranged by size:
3306 1342 32
PpuMI rG'GwC_Cy
Cuts at: 0 1511 1541 1571 1661 2165 2195 3045 4305
Size: 1511 30 30 90 504 30 850 1260
Cuts at: 4305 4680
Size: 375
Fragments arranged by size:
1511 1260 850 504 375 90 30 30
30
PshAI GACnn'nnGTC
Cuts at: 0 2407 4680
Size: 2407 2273
PspOMI G'GGCC_C
Cuts at: 0 791 851 911 941 971 1001 1031 1061
Size: 791 60 60 30 30 30 30 30
Cuts at: 1061 1091 1181 1241 1271 1301 1331 1361 1391
Size: 30 90 60 30 30 30 30 30
Cuts at: 1391 1421 1451 1481 1601 1631 1691 1721 1751
Size: 30 30 30 120 30 60 30 30
Cuts at: 1751 1781 1811 1871 1901 2362 4265 4680
Size: 30 30 60 30 461 1903 415
Fragments arranged by size:
1903 791 461 415 120 90 60 60
60 60 60 30 30 30 30 30
30 30 30 30 30 30 30 30
30 30 30 30 30 30 30
PstI C_TGCA'G
Cuts at: 0 2780 2846 3053 3059 3092 3710 3824 3887
Size: 2780 66 207 6 33 618 114 63
Cuts at: 3887 4562 4625 4680
Size: 675 63 55
Fragments arranged by size:
2780 675 618 207 114 66 63 63
55 33 6
PvuII CAG'CTG
Cuts at: 0 454 2227 2553 2691 2775 2808 2832 3028
Size: 454 1773 326 138 84 33 24 196
Cuts at: 3028 3054 4317 4329 4494 4590 4632 4680
Size: 26 1263 12 165 96 42 48
Fragments arranged by size:
1773 1263 454 326 196 165 138 96
84 48 42 33 26 24 12
SacI G_AGCT'C
Cuts at: 0 2079 2627 2942 3467 3950 4680
Size: 2079 548 315 525 483 730
Fragments arranged by size:
2079 730 548 525 483 315
SacII CC_GC'GG
Cuts at: 0 3761 4680
Size: 3761 919
SalI G'TCGA_C
Cuts at: 0 84 4680
Size: 84 4596
SanDI GG'GwC_CC
Cuts at: 0 1511 1541 1571 1661 2165 2195 4680
Size: 1511 30 30 90 504 30 2485
Fragments arranged by size:
2485 1511 504 90 30 30 30
SapI GCTCTTCn'nnn_
Cuts at: 0 3883 4228 4680
Size: 3883 345 452
Fragments arranged by size:
3883 452 345
SexAI A'CCwGG_T
Cuts at: 0 4025 4680
Size: 4025 655
SfcI C'TryA_G
Cuts at: 0 464 2776 2842 3049 3055 3088 3706 3820
Size: 464 2312 66 207 6 33 618 114
Cuts at: 3820 3883 4558 4621 4680
Size: 63 675 63 59
Fragments arranged by size:
2312 675 618 464 207 114 66 63
63 59 33 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1000 1030 1060 1090 1240 1270 1330 1360
Size: 1000 30 30 30 150 30 60 30
Cuts at: 1360 1390 1420 1450 1480 1600 1630 1690 1720
Size: 30 30 30 30 120 30 60 30
Cuts at: 1720 1750 1780 1810 3556 4680
Size: 30 30 30 1746 1124
Fragments arranged by size:
1746 1124 1000 150 120 60 60 30
30 30 30 30 30 30 30 30
30 30 30 30 30
SfoI GGC'GCC
Cuts at: 0 3933 4289 4680
Size: 3933 356 391
Fragments arranged by size:
3933 391 356
SmaI CCC'GGG
Cuts at: 0 2701 3961 4680
Size: 2701 1260 719
Fragments arranged by size:
2701 1260 719
SmlI C'TyrA_G
Cuts at: 0 3415 4680
Size: 3415 1265
SphI G_CATG'C
Cuts at: 0 2736 3873 4680
Size: 2736 1137 807
Fragments arranged by size:
2736 1137 807
StuI AGG'CCT
Cuts at: 0 3559 4486 4680
Size: 3559 927 194
Fragments arranged by size:
3559 927 194
StyI C'CwwG_G
Cuts at: 0 23 407 521 560 711 741 771 2055
Size: 23 384 114 39 151 30 30 1284
Cuts at: 2055 2205 3020 3530 3593 3638 3923 4109 4680
Size: 150 815 510 63 45 285 186 571
Fragments arranged by size:
1284 815 571 510 384 285 186 151
150 114 63 45 39 30 30 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 544 4680
Size: 544 4136
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3898 3930 4680
Size: 3898 32 750
Fragments arranged by size:
3898 750 32
Tth111I GACn'n_nGTC
Cuts at: 0 2250 3106 4680
Size: 2250 856 1574
Fragments arranged by size:
2250 1574 856
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2829 2892 3537 4246 4680
Size: 2829 63 645 709 434
Fragments arranged by size:
2829 709 645 434 63
XmaI C'CCGG_G
Cuts at: 0 2699 3959 4680
Size: 2699 1260 721
Fragments arranged by size:
2699 1260 721
XmnI GAAnn'nnTTC
Cuts at: 0 3321 4680
Size: 3321 1359
ZraI GAC'GTC
Cuts at: 0 3372 4680
Size: 3372 1308
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AleI AlwNI ApaI
ApoI AscI AvaI BanI BanII BbeI BbsI BbvCI
BcgI BclI BglI BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NotI NspI PasI PflMI PfoI PmlI PpiI PpuMI
PshAI PspOMI PstI PvuII SacI SacII SalI SanDI
SapI SexAI SfcI SfiI SfoI SmaI SmlI SphI
StuI StyI TaqII TstI Tth111I XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI
ApaLI AseI AsiSI AvrII BaeI BamHI BciVI BfrBI
BglII BmgBI BmtI BsiWI BsmI BsrBI BsrDI BsrGI
BstZ17I Bsu36I ClaI CspCI DraI DrdI EcoRV FseI
FspAI KpnI MfeI MluI NheI NruI NsiI PacI
PciI PmeI PsiI PspXI PsrI PvuI RsrII SbfI
ScaI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
TatI XbaI XhoI