Restriction map for
pF1KSDA1400
(Linear) (Six-base) MAPSORT of: KIAA1400.seq Check: 5570 from: 1 to: 3120
KIAA1400.orf
With 182 enzymes: SgfI *
February 28, 2007 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1164 3120
Size: 1164 1956
AccI GT'mk_AC
Cuts at: 0 761 1400 3120
Size: 761 639 1720
Fragments arranged by size:
1720 761 639
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 849 1062 1599 1627 3120
Size: 134 715 213 537 28 1493
Fragments arranged by size:
1493 715 537 213 134 28
AflII C'TTAA_G
Cuts at: 0 2509 3120
Size: 2509 611
AflIII A'CryG_T
Cuts at: 0 1406 3120
Size: 1406 1714
AgeI A'CCGG_T
Cuts at: 0 2572 3120
Size: 2572 548
AleI CACnn'nnGTG
Cuts at: 0 97 802 1126 1804 2020 2953 3120
Size: 97 705 324 678 216 933 167
Fragments arranged by size:
933 705 678 324 216 167 97
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2932 3120
Size: 2932 188
AlwNI CAG_nnn'CTG
Cuts at: 0 1659 2950 3120
Size: 1659 1291 170
Fragments arranged by size:
1659 1291 170
ApaI G_GGCC'C
Cuts at: 0 1003 3120
Size: 1003 2117
ApaLI G'TGCA_C
Cuts at: 0 586 3120
Size: 586 2534
AseI AT'TA_AT
Cuts at: 0 344 3120
Size: 344 2776
AvaI C'yCGr_G
Cuts at: 0 884 1304 1507 1634 2324 3120
Size: 884 420 203 127 690 796
Fragments arranged by size:
884 796 690 420 203 127
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1342 1375 3120
Size: 1342 33 1745
Fragments arranged by size:
1745 1342 33
BanI G'GyrC_C
Cuts at: 0 165 454 1113 1435 1477 1725 2041 2403
Size: 165 289 659 322 42 248 316 362
Cuts at: 2403 3120
Size: 717
Fragments arranged by size:
717 659 362 322 316 289 248 165
42
BanII G_rGCy'C
Cuts at: 0 265 964 1003 2054 2170 2377 2654 3120
Size: 265 699 39 1051 116 207 277 466
Fragments arranged by size:
1051 699 466 277 265 207 116 39
BbeI G_GCGC'C
Cuts at: 0 1117 1439 1481 1729 2045 3120
Size: 1117 322 42 248 316 1075
Fragments arranged by size:
1117 1075 322 316 248 42
BbsI GAAGACnn'nnnn_
Cuts at: 0 1211 1527 3120
Size: 1211 316 1593
Fragments arranged by size:
1593 1211 316
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 519 553 3120
Size: 519 34 2567
Fragments arranged by size:
2567 519 34
BclI T'GATC_A
Cuts at: 0 2763 3120
Size: 2763 357
BglI GCCn_nnn'nGGC
Cuts at: 0 1723 3120
Size: 1723 1397
BglII A'GATC_T
Cuts at: 0 117 3120
Size: 117 3003
BlpI GC'TnA_GC
Cuts at: 0 542 2653 3120
Size: 542 2111 467
Fragments arranged by size:
2111 542 467
Bme1580I G_kGCm'C
Cuts at: 0 170 457 590 743 1003 2787 3120
Size: 170 287 133 153 260 1784 333
Fragments arranged by size:
1784 333 287 260 170 153 133
BmgBI CAC'GTC
Cuts at: 0 450 510 1200 1407 3120
Size: 450 60 690 207 1713
Fragments arranged by size:
1713 690 450 207 60
BmrI ACTGGGnnnn_n'
Cuts at: 0 958 1201 2099 2381 2781 3120
Size: 958 243 898 282 400 339
Fragments arranged by size:
958 898 400 339 282 243
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2668 3120
Size: 2668 452
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 304 316 328 574 949 1736 2951
Size: 223 81 12 12 246 375 787 1215
Cuts at: 2951 3058 3120
Size: 107 62
Fragments arranged by size:
1215 787 375 246 223 107 81 62
12 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 159 1198 3120
Size: 159 1039 1922
Fragments arranged by size:
1922 1039 159
BsaAI yAC'GTr
Cuts at: 0 222 597 981 3120
Size: 222 375 384 2139
Fragments arranged by size:
2139 384 375 222
BsaBI GATnn'nnATC
Cuts at: 0 2762 3120
Size: 2762 358
BsaHI Gr'CG_yC
Cuts at: 0 1114 1436 1478 1726 2042 2889 3120
Size: 1114 322 42 248 316 847 231
Fragments arranged by size:
1114 847 322 316 248 231 42
BsaWI w'CCGG_w
Cuts at: 0 2572 3120
Size: 2572 548
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 91 632 644 1177 2428 3120
Size: 91 541 12 533 1251 692
Fragments arranged by size:
1251 692 541 533 91 12
BseYI C'CCAG_C
Cuts at: 0 53 677 1487 1949 2975 3120
Size: 53 624 810 462 1026 145
Fragments arranged by size:
1026 810 624 462 145 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 532 1198 2389 3120
Size: 262 270 666 1191 731
Fragments arranged by size:
1191 731 666 270 262
BsiEI CG_ry'CG
Cuts at: 0 1467 3120
Size: 1467 1653
BsiHKAI G_wGCw'C
Cuts at: 0 590 2096 2377 2549 2654 3120
Size: 590 1506 281 172 105 466
Fragments arranged by size:
1506 590 466 281 172 105
BsmI GAATG_Cn'
Cuts at: 0 436 2695 3120
Size: 436 2259 425
Fragments arranged by size:
2259 436 425
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1190 3120
Size: 1190 1930
Bsp1286I G_dGCh'C
Cuts at: 0 170 265 457 590 743 964 1003 2054
Size: 170 95 192 133 153 221 39 1051
Cuts at: 2054 2096 2170 2377 2549 2654 2787 3120
Size: 42 74 207 172 105 133 333
Fragments arranged by size:
1051 333 221 207 192 172 170 153
133 133 105 95 74 42 39
BspHI T'CATG_A
Cuts at: 0 2766 3120
Size: 2766 354
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1164 1802 2314 3120
Size: 1164 638 512 806
Fragments arranged by size:
1164 806 638 512
BsrBI CCG'CTC
Cuts at: 0 1775 2072 2991 3120
Size: 1775 297 919 129
Fragments arranged by size:
1775 919 297 129
BsrFI r'CCGG_y
Cuts at: 0 691 1395 2572 3120
Size: 691 704 1177 548
Fragments arranged by size:
1177 704 691 548
BssHII G'CGCG_C
Cuts at: 0 2329 3120
Size: 2329 791
BssSI C'ACGA_G
Cuts at: 0 802 3120
Size: 802 2318
BstAPI GCAn_nnn'nTGC
Cuts at: 0 585 3120
Size: 585 2535
BstEII G'GTnAC_C
Cuts at: 0 1789 3120
Size: 1789 1331
BstXI CCAn_nnnn'nTGG
Cuts at: 0 526 996 2022 2794 3120
Size: 526 470 1026 772 326
Fragments arranged by size:
1026 772 526 470 326
BstYI r'GATC_y
Cuts at: 0 117 1517 2802 3120
Size: 117 1400 1285 318
Fragments arranged by size:
1400 1285 318 117
BtgI C'CryG_G
Cuts at: 0 605 1814 1986 2045 3120
Size: 605 1209 172 59 1075
Fragments arranged by size:
1209 1075 605 172 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2142 3120
Size: 2142 978
BtsI GCAGTG_nn'
Cuts at: 0 1020 1186 3120
Size: 1020 166 1934
Fragments arranged by size:
1934 1020 166
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3080 3115 3120
Size: 3080 35 5
Fragments arranged by size:
3080 35 5
DrdI GACnn_nn'nnGTC
Cuts at: 0 757 2481 3120
Size: 757 1724 639
Fragments arranged by size:
1724 757 639
EaeI y'GGCC_r
Cuts at: 0 1808 1934 2192 2207 2255 2313 3120
Size: 1808 126 258 15 48 58 807
Fragments arranged by size:
1808 807 258 126 58 48 15
EarI CTCTTCn'nnn_
Cuts at: 0 950 2812 3120
Size: 950 1862 308
Fragments arranged by size:
1862 950 308
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1198 1795 2093 2309 2475 3120
Size: 1198 597 298 216 166 645
Fragments arranged by size:
1198 645 597 298 216 166
EcoICRI GAG'CTC
Cuts at: 0 2375 2652 3120
Size: 2375 277 468
Fragments arranged by size:
2375 468 277
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 223 304 316 328 574 849 949
Size: 134 89 81 12 12 246 275 100
Cuts at: 949 1062 1599 1627 1736 2951 3058 3120
Size: 113 537 28 109 1215 107 62
Fragments arranged by size:
1215 537 275 246 134 113 109 107
100 89 81 62 28 12 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1733 3120
Size: 1733 1387
EcoO109I rG'GnC_Cy
Cuts at: 0 179 993 1000 3021 3120
Size: 179 814 7 2021 99
Fragments arranged by size:
2021 814 179 99 7
EcoRV GAT'ATC
Cuts at: 0 2598 3120
Size: 2598 522
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 200 1239 3120
Size: 200 1039 1881
Fragments arranged by size:
1881 1039 200
FspI TGC'GCA
Cuts at: 0 1019 1915 3120
Size: 1019 896 1205
Fragments arranged by size:
1205 1019 896
HaeII r_GCGC'y
Cuts at: 0 404 1117 1439 1460 1481 1660 1729 2045
Size: 404 713 322 21 21 179 69 316
Cuts at: 2045 2103 3082 3120
Size: 58 979 38
Fragments arranged by size:
979 713 404 322 316 179 69 58
38 21 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1141 1173 2587 2619 2794 2826 3120
Size: 1141 32 1414 32 175 32 294
Fragments arranged by size:
1414 1141 294 175 32 32 32
HincII GTy'rAC
Cuts at: 0 1680 2667 2685 3120
Size: 1680 987 18 435
Fragments arranged by size:
1680 987 435 18
HpaI GTT'AAC
Cuts at: 0 2685 3120
Size: 2685 435
KasI G'GCGC_C
Cuts at: 0 1113 1435 1477 1725 2041 3120
Size: 1113 322 42 248 316 1079
Fragments arranged by size:
1113 1079 322 316 248 42
MfeI C'AATT_G
Cuts at: 0 2814 3120
Size: 2814 306
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 926 1340 2630 2648 3120
Size: 926 414 1290 18 472
Fragments arranged by size:
1290 926 472 414 18
MscI TGG'CCA
Cuts at: 0 2194 2257 3120
Size: 2194 63 863
Fragments arranged by size:
2194 863 63
MslI CAynn'nnrTG
Cuts at: 0 97 715 802 1126 1804 2020 2200 2365
Size: 97 618 87 324 678 216 180 165
Cuts at: 2365 2953 3120
Size: 588 167
Fragments arranged by size:
678 618 588 324 216 180 167 165
97 87
MspA1I CmG'CkG
Cuts at: 0 607 1605 1953 2031 2794 3120
Size: 607 998 348 78 763 326
Fragments arranged by size:
998 763 607 348 326 78
NarI GG'CG_CC
Cuts at: 0 1114 1436 1478 1726 2042 3120
Size: 1114 322 42 248 316 1078
Fragments arranged by size:
1114 1078 322 316 248 42
NcoI C'CATG_G
Cuts at: 0 1986 3120
Size: 1986 1134
NspI r_CATG'y
Cuts at: 0 2923 3120
Size: 2923 197
PasI CC'CwG_GG
Cuts at: 0 519 2055 3120
Size: 519 1536 1065
Fragments arranged by size:
1536 1065 519
PflMI CCAn_nnn'nTGG
Cuts at: 0 1659 3120
Size: 1659 1461
PfoI T'CCnGG_A
Cuts at: 0 365 2699 3120
Size: 365 2334 421
Fragments arranged by size:
2334 421 365
PpuMI rG'GwC_Cy
Cuts at: 0 179 993 3120
Size: 179 814 2127
Fragments arranged by size:
2127 814 179
PshAI GACnn'nnGTC
Cuts at: 0 2732 3120
Size: 2732 388
PspOMI G'GGCC_C
Cuts at: 0 999 3120
Size: 999 2121
PspXI vC'TCGA_Gb
Cuts at: 0 1507 3120
Size: 1507 1613
PstI C_TGCA'G
Cuts at: 0 2521 3120
Size: 2521 599
PvuII CAG'CTG
Cuts at: 0 1605 2031 2794 3120
Size: 1605 426 763 326
Fragments arranged by size:
1605 763 426 326
SacI G_AGCT'C
Cuts at: 0 2377 2654 3120
Size: 2377 277 466
Fragments arranged by size:
2377 466 277
SacII CC_GC'GG
Cuts at: 0 608 3120
Size: 608 2512
ScaI AGT'ACT
Cuts at: 0 1043 3120
Size: 1043 2077
SfcI C'TryA_G
Cuts at: 0 1295 2517 3120
Size: 1295 1222 603
Fragments arranged by size:
1295 1222 603
SfoI GGC'GCC
Cuts at: 0 1115 1437 1479 1727 2043 3120
Size: 1115 322 42 248 316 1077
Fragments arranged by size:
1115 1077 322 316 248 42
SmaI CCC'GGG
Cuts at: 0 886 1636 2326 3120
Size: 886 750 690 794
Fragments arranged by size:
886 794 750 690
SmlI C'TyrA_G
Cuts at: 0 174 1213 1507 2509 3120
Size: 174 1039 294 1002 611
Fragments arranged by size:
1039 1002 611 294 174
SrfI GCCC'GGGC
Cuts at: 0 2326 3120
Size: 2326 794
StuI AGG'CCT
Cuts at: 0 668 3120
Size: 668 2452
StyI C'CwwG_G
Cuts at: 0 425 989 1986 3120
Size: 425 564 997 1134
Fragments arranged by size:
1134 997 564 425
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 584 1280 2509 3120
Size: 584 696 1229 611
Fragments arranged by size:
1229 696 611 584
TatI w'GTAC_w
Cuts at: 0 857 1041 3120
Size: 857 184 2079
Fragments arranged by size:
2079 857 184
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1913 1945 3120
Size: 1913 32 1175
Fragments arranged by size:
1913 1175 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 717 1660 2016 2202 3120
Size: 717 943 356 186 918
Fragments arranged by size:
943 918 717 356 186
XhoI C'TCGA_G
Cuts at: 0 1507 3120
Size: 1507 1613
XmaI C'CCGG_G
Cuts at: 0 884 1634 2324 3120
Size: 884 750 690 796
Fragments arranged by size:
884 796 750 690
XmnI GAAnn'nnTTC
Cuts at: 0 1612 3120
Size: 1612 1508
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI AseI AvaI BaeI BanI BanII
BbeI BbsI BcgI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrFI BssHII BssSI BstAPI
BstEII BstXI BstYI BtgI BtgZI BtsI CspCI DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV
FalI FspI HaeII Hin4I HincII HpaI KasI MfeI
MmeI MscI MslI MspA1I NarI NcoI NspI PasI
PflMI PfoI PpuMI PshAI PspOMI PspXI PstI PvuII
SacI SacII ScaI SfcI SfoI SmaI SmlI SrfI
StuI StyI TaqII TatI TstI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AhdI AloI ApoI AscI
AsiSI AvrII BamHI BbvCI BciVI BfrBI BmtI Bpu10I
BsaI BsaXI BsiWI BspEI BsrDI BsrGI BstBI BstZ17I
Bsu36I ClaI DraI DraIII EagI EcoRI FseI FspAI
HindIII KpnI MluI NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PsiI
PsrI PvuI RsrII SalI SanDI SapI SbfI SexAI
SfiI SgfI SgrAI SnaBI SpeI SphI SspI SwaI
Tth111I XbaI ZraI