Restriction map for
pF1KA1401
(Linear) (Six-base) MAPSORT of: KIAA1401.seq Check: 5310 from: 1 to: 2412
KIAA1401.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AccI GT'mk_AC
Cuts at: 0 152 1050 2412
Size: 152 898 1362
Fragments arranged by size:
1362 898 152
Acc65I G'GTAC_C
Cuts at: 0 2236 2412
Size: 2236 176
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 610 1701 1709 2412
Size: 610 1091 8 703
Fragments arranged by size:
1091 703 610 8
AfeI AGC'GCT
Cuts at: 0 640 2412
Size: 640 1772
AflII C'TTAA_G
Cuts at: 0 1516 2412
Size: 1516 896
AflIII A'CryG_T
Cuts at: 0 2144 2412
Size: 2144 268
AgeI A'CCGG_T
Cuts at: 0 505 2412
Size: 505 1907
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2038 2412
Size: 2038 374
AlwNI CAG_nnn'CTG
Cuts at: 0 255 846 1045 2412
Size: 255 591 199 1367
Fragments arranged by size:
1367 591 255 199
ApoI r'AATT_y
Cuts at: 0 278 818 1490 2114 2412
Size: 278 540 672 624 298
Fragments arranged by size:
672 624 540 298 278
BamHI G'GATC_C
Cuts at: 0 1542 2412
Size: 1542 870
BanI G'GyrC_C
Cuts at: 0 261 808 2236 2412
Size: 261 547 1428 176
Fragments arranged by size:
1428 547 261 176
BanII G_rGCy'C
Cuts at: 0 1841 2412
Size: 1841 571
BbsI GAAGACnn'nnnn_
Cuts at: 0 2310 2412
Size: 2310 102
BbvCI CC'TCA_GC
Cuts at: 0 408 1400 1655 2412
Size: 408 992 255 757
Fragments arranged by size:
992 757 408 255
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 49 83 2412
Size: 49 34 2329
Fragments arranged by size:
2329 49 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1951 2412
Size: 1951 461
BlpI GC'TnA_GC
Cuts at: 0 1126 2412
Size: 1126 1286
Bme1580I G_kGCm'C
Cuts at: 0 266 379 811 2412
Size: 266 113 432 1601
Fragments arranged by size:
1601 432 266 113
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2229 2412
Size: 2229 183
Bpu10I CC'TnA_GC
Cuts at: 0 118 408 536 697 1400 1655 2412
Size: 118 290 128 161 703 255 757
Fragments arranged by size:
757 703 290 255 161 128 118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 10 1547 2267 2381 2412
Size: 10 1537 720 114 31
Fragments arranged by size:
1537 720 114 31 10
BsaI GGTCTCn'nnnn_
Cuts at: 0 911 2412
Size: 911 1501
BsaWI w'CCGG_w
Cuts at: 0 505 1526 2412
Size: 505 1021 886
Fragments arranged by size:
1021 886 505
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1643 1673 1965 1995 2412
Size: 1643 30 292 30 417
Fragments arranged by size:
1643 417 292 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 233 649 1132 1135 1306 2412
Size: 233 416 483 3 171 1106
Fragments arranged by size:
1106 483 416 233 171 3
BseYI C'CCAG_C
Cuts at: 0 222 494 2412
Size: 222 272 1918
Fragments arranged by size:
1918 272 222
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 253 412 2412
Size: 62 191 159 2000
Fragments arranged by size:
2000 191 159 62
BsiHKAI G_wGCw'C
Cuts at: 0 1841 2412
Size: 1841 571
BsmI GAATG_Cn'
Cuts at: 0 2027 2412
Size: 2027 385
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1704 2412
Size: 1704 708
Bsp1286I G_dGCh'C
Cuts at: 0 266 379 811 1841 2412
Size: 266 113 432 1030 571
Fragments arranged by size:
1030 571 432 266 113
BspEI T'CCGG_A
Cuts at: 0 1526 2412
Size: 1526 886
BspHI T'CATG_A
Cuts at: 0 1758 2412
Size: 1758 654
BsrBI CCG'CTC
Cuts at: 0 24 2412
Size: 24 2388
BsrDI GCAATG_nn'
Cuts at: 0 980 2022 2033 2412
Size: 980 1042 11 379
Fragments arranged by size:
1042 980 379 11
BsrFI r'CCGG_y
Cuts at: 0 505 2412
Size: 505 1907
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1237 2363 2412
Size: 1237 1126 49
Fragments arranged by size:
1237 1126 49
BstYI r'GATC_y
Cuts at: 0 73 423 1083 1542 2061 2412
Size: 73 350 660 459 519 351
Fragments arranged by size:
660 519 459 351 350 73
Bsu36I CC'TnA_GG
Cuts at: 0 1547 2412
Size: 1547 865
BtgI C'CryG_G
Cuts at: 0 1537 2241 2412
Size: 1537 704 171
Fragments arranged by size:
1537 704 171
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 148 2412
Size: 148 2264
BtsI GCAGTG_nn'
Cuts at: 0 635 1895 2412
Size: 635 1260 517
Fragments arranged by size:
1260 635 517
DraI TTT'AAA
Cuts at: 0 932 1623 2412
Size: 932 691 789
Fragments arranged by size:
932 789 691
DraIII CAC_nnn'GTG
Cuts at: 0 435 2412
Size: 435 1977
DrdI GACnn_nn'nnGTC
Cuts at: 0 2074 2412
Size: 2074 338
EaeI y'GGCC_r
Cuts at: 0 2244 2412
Size: 2244 168
EarI CTCTTCn'nnn_
Cuts at: 0 208 785 1316 1436 1829 2412
Size: 208 577 531 120 393 583
Fragments arranged by size:
583 577 531 393 208 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 68 2412
Size: 68 2344
EcoICRI GAG'CTC
Cuts at: 0 1839 2412
Size: 1839 573
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 610 1701 1709 2229 2412
Size: 610 1091 8 520 183
Fragments arranged by size:
1091 610 520 183 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 289 411 1033 2412
Size: 289 122 622 1379
Fragments arranged by size:
1379 622 289 122
EcoO109I rG'GnC_Cy
Cuts at: 0 540 960 1176 2412
Size: 540 420 216 1236
Fragments arranged by size:
1236 540 420 216
EcoRI G'AATT_C
Cuts at: 0 1490 2412
Size: 1490 922
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1036 1161 2412
Size: 1036 125 1251
Fragments arranged by size:
1251 1036 125
HaeII r_GCGC'y
Cuts at: 0 642 1866 2412
Size: 642 1224 546
Fragments arranged by size:
1224 642 546
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1282 1314 2088 2120 2204 2236 2322 2354
Size: 1282 32 774 32 84 32 86 32
Cuts at: 2354 2412
Size: 58
Fragments arranged by size:
1282 774 86 84 58 32 32 32
32
HincII GTy'rAC
Cuts at: 0 153 2412
Size: 153 2259
KpnI G_GTAC'C
Cuts at: 0 2240 2412
Size: 2240 172
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 855 1394 2412
Size: 571 284 539 1018
Fragments arranged by size:
1018 571 539 284
MscI TGG'CCA
Cuts at: 0 2246 2412
Size: 2246 166
MslI CAynn'nnrTG
Cuts at: 0 338 973 1536 2412
Size: 338 635 563 876
Fragments arranged by size:
876 635 563 338
MspA1I CmG'CkG
Cuts at: 0 84 222 303 1903 2412
Size: 84 138 81 1600 509
Fragments arranged by size:
1600 509 138 84 81
NcoI C'CATG_G
Cuts at: 0 1537 2241 2412
Size: 1537 704 171
Fragments arranged by size:
1537 704 171
NdeI CA'TA_TG
Cuts at: 0 2343 2412
Size: 2343 69
NspI r_CATG'y
Cuts at: 0 2148 2412
Size: 2148 264
PciI A'CATG_T
Cuts at: 0 2144 2412
Size: 2144 268
PpuMI rG'GwC_Cy
Cuts at: 0 960 1176 2412
Size: 960 216 1236
Fragments arranged by size:
1236 960 216
PstI C_TGCA'G
Cuts at: 0 1902 2412
Size: 1902 510
PvuII CAG'CTG
Cuts at: 0 222 303 2412
Size: 222 81 2109
Fragments arranged by size:
2109 222 81
SacI G_AGCT'C
Cuts at: 0 1841 2412
Size: 1841 571
SalI G'TCGA_C
Cuts at: 0 151 2412
Size: 151 2261
SapI GCTCTTCn'nnn_
Cuts at: 0 1829 2412
Size: 1829 583
ScaI AGT'ACT
Cuts at: 0 2294 2412
Size: 2294 118
SfcI C'TryA_G
Cuts at: 0 998 1898 2039 2412
Size: 998 900 141 373
Fragments arranged by size:
998 900 373 141
SgrAI Cr'CCGG_yG
Cuts at: 0 505 2412
Size: 505 1907
SmlI C'TyrA_G
Cuts at: 0 25 1516 1562 2282 2396 2412
Size: 25 1491 46 720 114 16
Fragments arranged by size:
1491 720 114 46 25 16
SspI AAT'ATT
Cuts at: 0 1580 2412
Size: 1580 832
StuI AGG'CCT
Cuts at: 0 1514 2412
Size: 1514 898
StyI C'CwwG_G
Cuts at: 0 1537 1829 2241 2412
Size: 1537 292 412 171
Fragments arranged by size:
1537 412 292 171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 214 370 2412
Size: 214 156 2042
Fragments arranged by size:
2042 214 156
TatI w'GTAC_w
Cuts at: 0 330 2292 2412
Size: 330 1962 120
Fragments arranged by size:
1962 330 120
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 548 580 1965 1997 2155 2187 2412
Size: 548 32 1385 32 158 32 225
Fragments arranged by size:
1385 548 225 158 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 491 574 2412
Size: 491 83 1838
Fragments arranged by size:
1838 491 83
XmnI GAAnn'nnTTC
Cuts at: 0 1556 2412
Size: 1556 856
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflII AflIII AgeI AlfI
AlwNI ApoI BamHI BanI BanII BbsI BbvCI BcgI
BglI BlpI Bme1580I BpmI Bpu10I BpuEI BsaI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BsrBI BsrDI BsrFI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII
Hin4I HincII KpnI MmeI MscI MslI MspA1I NcoI
NdeI NspI PciI PpuMI PstI PvuII SacI SalI
SapI ScaI SfcI SgrAI SmlI SspI StuI StyI
TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AhdI AleI AloI ApaI ApaLI
AscI AseI AsiSI AvaI AvrII BaeI BbeI BciVI
BclI BfrBI BglII BmgBI BmrI BmtI BplI BsaAI
BsaBI BsaHI BsiEI BsiWI BspMI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I ClaI CspCI EagI EcoRV
FseI FspI FspAI HindIII HpaI KasI MfeI MluI
NaeI NarI NgoMIV NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SanDI SbfI
SexAI SfiI SfoI SgfI SmaI SnaBI SpeI SphI
SrfI SwaI Tth111I XbaI XhoI XmaI ZraI