Restriction map for
pF1KA1402
(Linear) (Six-base) MAPSORT of: KIAA1402.seq Check: 8364 from: 1 to: 2316
KIAA1402.orf
With 182 enzymes: SgfI *
November 13, 2006 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 429 2050 2316
Size: 429 1621 266
Fragments arranged by size:
1621 429 266
AccI GT'mk_AC
Cuts at: 0 1583 2316
Size: 1583 733
Acc65I G'GTAC_C
Cuts at: 0 931 1664 2316
Size: 931 733 652
Fragments arranged by size:
931 733 652
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 945 1514 2316
Size: 945 569 802
Fragments arranged by size:
945 802 569
AfeI AGC'GCT
Cuts at: 0 38 966 2316
Size: 38 928 1350
Fragments arranged by size:
1350 928 38
AleI CACnn'nnGTG
Cuts at: 0 556 1462 2316
Size: 556 906 854
Fragments arranged by size:
906 854 556
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2021 2316
Size: 2021 295
AlwNI CAG_nnn'CTG
Cuts at: 0 1284 2316
Size: 1284 1032
ApaI G_GGCC'C
Cuts at: 0 420 1383 1802 1916 2316
Size: 420 963 419 114 400
Fragments arranged by size:
963 420 419 400 114
ApaLI G'TGCA_C
Cuts at: 0 913 2316
Size: 913 1403
AvaI C'yCGr_G
Cuts at: 0 326 419 1460 1519 1917 1964 2045 2316
Size: 326 93 1041 59 398 47 81 271
Fragments arranged by size:
1041 398 326 271 93 81 59 47
BamHI G'GATC_C
Cuts at: 0 98 2316
Size: 98 2218
BanI G'GyrC_C
Cuts at: 0 931 1370 1664 2316
Size: 931 439 294 652
Fragments arranged by size:
931 652 439 294
BanII G_rGCy'C
Cuts at: 0 13 177 292 420 426 889 1079 1170
Size: 13 164 115 128 6 463 190 91
Cuts at: 1170 1383 1614 1802 1916 1974 2052 2141 2316
Size: 213 231 188 114 58 78 89 175
Fragments arranged by size:
463 231 213 190 188 175 164 128
115 114 91 89 78 58 13 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 203 2316
Size: 203 2113
BbvCI CC'TCA_GC
Cuts at: 0 595 2239 2316
Size: 595 1644 77
Fragments arranged by size:
1644 595 77
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 820 854 1225 1259 1510 1544 2316
Size: 820 34 371 34 251 34 772
Fragments arranged by size:
820 772 371 251 34 34 34
BfrBI ATG'CAT
Cuts at: 0 1561 2316
Size: 1561 755
BglI GCCn_nnn'nGGC
Cuts at: 0 471 1074 2056 2275 2316
Size: 471 603 982 219 41
Fragments arranged by size:
982 603 471 219 41
BlpI GC'TnA_GC
Cuts at: 0 1060 2316
Size: 1060 1256
Bme1580I G_kGCm'C
Cuts at: 0 420 759 917 1180 1373 1383 1802 1916
Size: 420 339 158 263 193 10 419 114
Cuts at: 1916 2271 2316
Size: 355 45
Fragments arranged by size:
420 419 355 339 263 193 158 114
45 10
BmgBI CAC'GTC
Cuts at: 0 1206 1734 2316
Size: 1206 528 582
Fragments arranged by size:
1206 582 528
BmrI ACTGGGnnnn_n'
Cuts at: 0 413 2316
Size: 413 1903
BmtI G_CTAG'C
Cuts at: 0 1196 2316
Size: 1196 1120
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 306 766 2316
Size: 306 460 1550
Fragments arranged by size:
1550 460 306
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 991 1252 1564 1598 2116 2125 2269
Size: 410 581 261 312 34 518 9 144
Cuts at: 2269 2316
Size: 47
Fragments arranged by size:
581 518 410 312 261 144 47 34
9
Bpu10I CC'TnA_GC
Cuts at: 0 76 259 595 2239 2316
Size: 76 183 336 1644 77
Fragments arranged by size:
1644 336 183 77 76
BsaI GGTCTCn'nnnn_
Cuts at: 0 478 1743 2316
Size: 478 1265 573
Fragments arranged by size:
1265 573 478
BsaHI Gr'CG_yC
Cuts at: 0 803 2316
Size: 803 1513
BsaWI w'CCGG_w
Cuts at: 0 1019 2126 2316
Size: 1019 1107 190
Fragments arranged by size:
1107 1019 190
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 686 716 907 937 1135 1165 1924 1954
Size: 686 30 191 30 198 30 759 30
Cuts at: 1954 2316
Size: 362
Fragments arranged by size:
759 686 362 198 191 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 161 646 755 2098 2316
Size: 161 485 109 1343 218
Fragments arranged by size:
1343 485 218 161 109
BseYI C'CCAG_C
Cuts at: 0 285 1116 1121 1311 2270 2316
Size: 285 831 5 190 959 46
Fragments arranged by size:
959 831 285 190 46 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 580 1178 1486 2194 2316
Size: 90 490 598 308 708 122
Fragments arranged by size:
708 598 490 308 122 90
BsiHKAI G_wGCw'C
Cuts at: 0 13 177 260 889 917 1614 2316
Size: 13 164 83 629 28 697 702
Fragments arranged by size:
702 697 629 164 83 28 13
BsiWI C'GTAC_G
Cuts at: 0 364 2316
Size: 364 1952
BsmI GAATG_Cn'
Cuts at: 0 1833 2316
Size: 1833 483
Bsp1286I G_dGCh'C
Cuts at: 0 13 177 260 292 420 426 759 889
Size: 13 164 83 32 128 6 333 130
Cuts at: 889 917 1079 1170 1180 1373 1383 1614 1802
Size: 28 162 91 10 193 10 231 188
Cuts at: 1802 1916 1974 2052 2141 2271 2316
Size: 114 58 78 89 130 45
Fragments arranged by size:
333 231 193 188 164 162 130 130
128 114 91 89 83 78 58 45
32 28 13 10 10 6
BspEI T'CCGG_A
Cuts at: 0 1019 2316
Size: 1019 1297
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 429 1349 2050 2316
Size: 429 920 701 266
Fragments arranged by size:
920 701 429 266
BsrBI CCG'CTC
Cuts at: 0 300 462 981 2316
Size: 300 162 519 1335
Fragments arranged by size:
1335 519 300 162
BsrDI GCAATG_nn'
Cuts at: 0 1561 2316
Size: 1561 755
BsrFI r'CCGG_y
Cuts at: 0 944 1373 1994 2316
Size: 944 429 621 322
Fragments arranged by size:
944 621 429 322
BssSI C'ACGA_G
Cuts at: 0 1550 1592 2316
Size: 1550 42 724
Fragments arranged by size:
1550 724 42
BstAPI GCAn_nnn'nTGC
Cuts at: 0 583 1284 1473 2316
Size: 583 701 189 843
Fragments arranged by size:
843 701 583 189
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1542 2316
Size: 1542 774
BstYI r'GATC_y
Cuts at: 0 98 117 1016 2316
Size: 98 19 899 1300
Fragments arranged by size:
1300 899 98 19
BtgI C'CryG_G
Cuts at: 0 350 1605 2203 2316
Size: 350 1255 598 113
Fragments arranged by size:
1255 598 350 113
DraIII CAC_nnn'GTG
Cuts at: 0 1366 1755 1784 2316
Size: 1366 389 29 532
Fragments arranged by size:
1366 532 389 29
EaeI y'GGCC_r
Cuts at: 0 31 347 588 718 854 1408 1602 2316
Size: 31 316 241 130 136 554 194 714
Fragments arranged by size:
714 554 316 241 194 136 130 31
EarI CTCTTCn'nnn_
Cuts at: 0 1771 2075 2316
Size: 1771 304 241
Fragments arranged by size:
1771 304 241
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1216 2316
Size: 1216 1100
EcoICRI GAG'CTC
Cuts at: 0 11 175 887 1612 2316
Size: 11 164 712 725 704
Fragments arranged by size:
725 712 704 164 11
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 945 991 1252 1514 1564 1598 2116
Size: 410 535 46 261 262 50 34 518
Cuts at: 2116 2125 2269 2316
Size: 9 144 47
Fragments arranged by size:
535 518 410 262 261 144 50 47
46 34 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 225 2316
Size: 225 2091
EcoO109I rG'GnC_Cy
Cuts at: 0 231 416 564 867 1380 1707 1912 1913
Size: 231 185 148 303 513 327 205 1
Cuts at: 1913 2316
Size: 403
Fragments arranged by size:
513 403 327 303 231 205 185 148
1
HaeII r_GCGC'y
Cuts at: 0 40 968 1251 1305 2316
Size: 40 928 283 54 1011
Fragments arranged by size:
1011 928 283 54 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 770 802 1723 1755 1924 1956 2316
Size: 770 32 921 32 169 32 360
Fragments arranged by size:
921 770 360 169 32 32 32
KpnI G_GTAC'C
Cuts at: 0 935 1668 2316
Size: 935 733 648
Fragments arranged by size:
935 733 648
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 956 1199 1328 2316
Size: 956 243 129 988
Fragments arranged by size:
988 956 243 129
MscI TGG'CCA
Cuts at: 0 590 856 2316
Size: 590 266 1460
Fragments arranged by size:
1460 590 266
MslI CAynn'nnrTG
Cuts at: 0 455 556 728 1462 1732 2316
Size: 455 101 172 734 270 584
Fragments arranged by size:
734 584 455 270 172 101
MspA1I CmG'CkG
Cuts at: 0 411 1470 1648 1817 2231 2274 2316
Size: 411 1059 178 169 414 43 42
Fragments arranged by size:
1059 414 411 178 169 43 42
NdeI CA'TA_TG
Cuts at: 0 555 2316
Size: 555 1761
NheI G'CTAG_C
Cuts at: 0 1192 2316
Size: 1192 1124
NsiI A_TGCA'T
Cuts at: 0 1563 2316
Size: 1563 753
NspI r_CATG'y
Cuts at: 0 1561 2316
Size: 1561 755
PasI CC'CwG_GG
Cuts at: 0 1669 1909 2316
Size: 1669 240 407
Fragments arranged by size:
1669 407 240
PflMI CCAn_nnn'nTGG
Cuts at: 0 1627 1784 2316
Size: 1627 157 532
Fragments arranged by size:
1627 532 157
PfoI T'CCnGG_A
Cuts at: 0 94 1541 1868 2316
Size: 94 1447 327 448
Fragments arranged by size:
1447 448 327 94
PpuMI rG'GwC_Cy
Cuts at: 0 231 867 2316
Size: 231 636 1449
Fragments arranged by size:
1449 636 231
PspOMI G'GGCC_C
Cuts at: 0 416 1379 1798 1912 2316
Size: 416 963 419 114 404
Fragments arranged by size:
963 419 416 404 114
PspXI vC'TCGA_Gb
Cuts at: 0 1519 2316
Size: 1519 797
PstI C_TGCA'G
Cuts at: 0 1010 1506 1644 2200 2316
Size: 1010 496 138 556 116
Fragments arranged by size:
1010 556 496 138 116
PvuII CAG'CTG
Cuts at: 0 1470 2274 2316
Size: 1470 804 42
Fragments arranged by size:
1470 804 42
SacI G_AGCT'C
Cuts at: 0 13 177 889 1614 2316
Size: 13 164 712 725 702
Fragments arranged by size:
725 712 702 164 13
SanDI GG'GwC_CC
Cuts at: 0 231 867 2316
Size: 231 636 1449
Fragments arranged by size:
1449 636 231
SfcI C'TryA_G
Cuts at: 0 134 225 1006 1183 1502 1640 1976 2196
Size: 134 91 781 177 319 138 336 220
Cuts at: 2196 2316
Size: 120
Fragments arranged by size:
781 336 319 220 177 138 134 120
91
SgrAI Cr'CCGG_yG
Cuts at: 0 1373 2316
Size: 1373 943
SmaI CCC'GGG
Cuts at: 0 421 1919 1966 2316
Size: 421 1498 47 350
Fragments arranged by size:
1498 421 350 47
SmlI C'TyrA_G
Cuts at: 0 1519 2316
Size: 1519 797
SrfI GCCC'GGGC
Cuts at: 0 421 2316
Size: 421 1895
StuI AGG'CCT
Cuts at: 0 1525 1794 1849 2316
Size: 1525 269 55 467
Fragments arranged by size:
1525 467 269 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1198 2316
Size: 1198 1118
TatI w'GTAC_w
Cuts at: 0 614 1328 2316
Size: 614 714 988
Fragments arranged by size:
988 714 614
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 686 718 907 939 1135 1167 2316
Size: 686 32 189 32 196 32 1149
Fragments arranged by size:
1149 686 196 189 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1735 2316
Size: 1735 581
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 348 2316
Size: 348 1968
XhoI C'TCGA_G
Cuts at: 0 1519 2316
Size: 1519 797
XmaI C'CCGG_G
Cuts at: 0 419 1917 1964 2316
Size: 419 1498 47 352
Fragments arranged by size:
1498 419 352 47
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AleI AlfI AlwNI
ApaI ApaLI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstXI BstYI BtgI
DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
HaeII Hin4I KpnI MmeI MscI MslI MspA1I NdeI
NheI NsiI NspI PasI PflMI PfoI PpuMI PspOMI
PspXI PstI PvuII SacI SanDI SfcI SgrAI SmaI
SmlI SrfI StuI TaqII TatI TstI Tth111I XcmI
XhoI XmaI
Enzymes that do not cut:
AatII AclI AflII AflIII AgeI AhdI AloI ApoI
AscI AseI AsiSI AvrII BaeI BbeI BciVI BclI
BglII BpuEI BsaAI BsaBI BsiEI BsmBI BspHI BsrGI
BssHII BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DrdI EagI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI MfeI MluI
NaeI NarI NcoI NgoMIV NotI NruI PacI PciI
PmeI PmlI PpiI PshAI PsiI PsrI PvuI RsrII
SacII SalI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SnaBI SpeI SphI SspI StyI SwaI XbaI
XmnI ZraI