Restriction map for
pF1KA1404
(Linear) (Six-base) MAPSORT of: KIAA1404.seq Check: 9763 from: 1 to: 5754
KIAA1404.orf
With 182 enzymes: SgfI *
November 13, 2006 11:20 ..
AccI GT'mk_AC
Cuts at: 0 1280 5754
Size: 1280 4474
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1330 1676 1807 2086 5057 5754
Size: 1330 346 131 279 2971 697
Fragments arranged by size:
2971 1330 697 346 279 131
AfeI AGC'GCT
Cuts at: 0 5233 5754
Size: 5233 521
AflII C'TTAA_G
Cuts at: 0 3292 4990 5754
Size: 3292 1698 764
Fragments arranged by size:
3292 1698 764
AflIII A'CryG_T
Cuts at: 0 3122 5392 5602 5754
Size: 3122 2270 210 152
Fragments arranged by size:
3122 2270 210 152
AgeI A'CCGG_T
Cuts at: 0 3992 4236 5754
Size: 3992 244 1518
Fragments arranged by size:
3992 1518 244
AleI CACnn'nnGTG
Cuts at: 0 961 4166 4419 5754
Size: 961 3205 253 1335
Fragments arranged by size:
3205 1335 961 253
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3526 4417 5754
Size: 3526 891 1337
Fragments arranged by size:
3526 1337 891
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3347 3379 4064 4096 5754
Size: 3347 32 685 32 1658
Fragments arranged by size:
3347 1658 685 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1197 1699 1995 2244 2404 2632 2962 3105
Size: 1197 502 296 249 160 228 330 143
Cuts at: 3105 3270 3708 5754
Size: 165 438 2046
Fragments arranged by size:
2046 1197 502 438 330 296 249 228
165 160 143
ApaLI G'TGCA_C
Cuts at: 0 1033 2211 4566 5754
Size: 1033 1178 2355 1188
Fragments arranged by size:
2355 1188 1178 1033
ApoI r'AATT_y
Cuts at: 0 39 643 1329 1705 2104 5754
Size: 39 604 686 376 399 3650
Fragments arranged by size:
3650 686 604 399 376 39
AseI AT'TA_AT
Cuts at: 0 1745 5754
Size: 1745 4009
AvaI C'yCGr_G
Cuts at: 0 3665 5754
Size: 3665 2089
AvrII C'CTAG_G
Cuts at: 0 160 5754
Size: 160 5594
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2133 2166 2803 2836 2848 2881 3803 3836
Size: 2133 33 637 33 12 33 922 33
Cuts at: 3836 5754
Size: 1918
Fragments arranged by size:
2133 1918 922 637 33 33 33 33
12
BanI G'GyrC_C
Cuts at: 0 2768 3717 3989 4041 4869 5754
Size: 2768 949 272 52 828 885
Fragments arranged by size:
2768 949 885 828 272 52
BanII G_rGCy'C
Cuts at: 0 518 557 4589 4957 5688 5754
Size: 518 39 4032 368 731 66
Fragments arranged by size:
4032 731 518 368 66 39
BbsI GAAGACnn'nnnn_
Cuts at: 0 18 445 762 986 1193 2026 2915 3311
Size: 18 427 317 224 207 833 889 396
Cuts at: 3311 4772 5045 5754
Size: 1461 273 709
Fragments arranged by size:
1461 889 833 709 427 396 317 273
224 207 18
BbvCI CC'TCA_GC
Cuts at: 0 2089 2848 5754
Size: 2089 759 2906
Fragments arranged by size:
2906 2089 759
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1075 1109 4586 4620 5754
Size: 1075 34 3477 34 1134
Fragments arranged by size:
3477 1134 1075 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 728 942 1101 3831 5754
Size: 728 214 159 2730 1923
Fragments arranged by size:
2730 1923 728 214 159
BclI T'GATC_A
Cuts at: 0 683 5754
Size: 683 5071
BfrBI ATG'CAT
Cuts at: 0 1364 3953 4436 5166 5754
Size: 1364 2589 483 730 588
Fragments arranged by size:
2589 1364 730 588 483
BglI GCCn_nnn'nGGC
Cuts at: 0 2541 5552 5754
Size: 2541 3011 202
Fragments arranged by size:
3011 2541 202
BglII A'GATC_T
Cuts at: 0 1425 5754
Size: 1425 4329
BlpI GC'TnA_GC
Cuts at: 0 4956 5754
Size: 4956 798
Bme1580I G_kGCm'C
Cuts at: 0 937 1037 2215 3229 3722 3992 4046 4151
Size: 937 100 1178 1014 493 270 54 105
Cuts at: 4151 4229 4450 4570 4874 5602 5754
Size: 78 221 120 304 728 152
Fragments arranged by size:
1178 1014 937 728 493 304 270 221
152 120 105 100 78 54
BmgBI CAC'GTC
Cuts at: 0 1551 5754
Size: 1551 4203
BmrI ACTGGGnnnn_n'
Cuts at: 0 439 631 1709 2280 2630 3107 5754
Size: 439 192 1078 571 350 477 2647
Fragments arranged by size:
2647 1078 571 477 439 350 192
BmtI G_CTAG'C
Cuts at: 0 1483 5754
Size: 1483 4271
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 32 1635 4143 5754
Size: 32 1603 2508 1611
Fragments arranged by size:
2508 1611 1603 32
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 736 1549 1924 2227 2368 2378 2887 3292
Size: 736 813 375 303 141 10 509 405
Cuts at: 3292 5023 5754
Size: 1731 731
Fragments arranged by size:
1731 813 736 731 509 405 375 303
141 10
Bpu10I CC'TnA_GC
Cuts at: 0 2089 2848 5754
Size: 2089 759 2906
Fragments arranged by size:
2906 2089 759
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 3055 3172 4201 5256 5404 5754
Size: 571 2484 117 1029 1055 148 350
Fragments arranged by size:
2484 1055 1029 571 350 148 117
BsaI GGTCTCn'nnnn_
Cuts at: 0 272 4660 5754
Size: 272 4388 1094
Fragments arranged by size:
4388 1094 272
BsaAI yAC'GTr
Cuts at: 0 5603 5754
Size: 5603 151
BsaBI GATnn'nnATC
Cuts at: 0 2470 3463 4930 5754
Size: 2470 993 1467 824
Fragments arranged by size:
2470 1467 993 824
BsaHI Gr'CG_yC
Cuts at: 0 3533 5754
Size: 3533 2221
BsaWI w'CCGG_w
Cuts at: 0 3992 4236 5754
Size: 3992 244 1518
Fragments arranged by size:
3992 1518 244
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 727 757 1624 1654 2040 2070 5754
Size: 727 30 867 30 386 30 3684
Fragments arranged by size:
3684 867 727 386 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 146 149 739 2449 2464 2467 2530
Size: 19 127 3 590 1710 15 3 63
Cuts at: 2530 3577 5008 5749 5754
Size: 1047 1431 741 5
Fragments arranged by size:
1710 1431 1047 741 590 127 63 19
15 5 3 3
BseYI C'CCAG_C
Cuts at: 0 2264 2537 2666 5687 5754
Size: 2264 273 129 3021 67
Fragments arranged by size:
3021 2264 273 129 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 808 985 1309 2317 2899 3888 4204 4777
Size: 808 177 324 1008 582 989 316 573
Cuts at: 4777 5446 5518 5754
Size: 669 72 236
Fragments arranged by size:
1008 989 808 669 582 573 324 316
236 177 72
BsiHKAI G_wGCw'C
Cuts at: 0 518 557 728 1037 2215 4570 5754
Size: 518 39 171 309 1178 2355 1184
Fragments arranged by size:
2355 1184 1178 518 309 171 39
BsmI GAATG_Cn'
Cuts at: 0 2334 5754
Size: 2334 3420
Bsp1286I G_dGCh'C
Cuts at: 0 518 557 728 937 1037 2215 3229 3722
Size: 518 39 171 209 100 1178 1014 493
Cuts at: 3722 3992 4046 4151 4229 4450 4570 4589 4874
Size: 270 54 105 78 221 120 19 285
Cuts at: 4874 4957 5602 5688 5754
Size: 83 645 86 66
Fragments arranged by size:
1178 1014 645 518 493 285 270 221
209 171 120 105 100 86 83 78
66 54 39 19
BspHI T'CATG_A
Cuts at: 0 2153 2187 2282 5754
Size: 2153 34 95 3472
Fragments arranged by size:
3472 2153 95 34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2252 2921 3455 5754
Size: 2252 669 534 2299
Fragments arranged by size:
2299 2252 669 534
BsrDI GCAATG_nn'
Cuts at: 0 3047 5556 5754
Size: 3047 2509 198
Fragments arranged by size:
3047 2509 198
BsrFI r'CCGG_y
Cuts at: 0 3992 4236 5754
Size: 3992 244 1518
Fragments arranged by size:
3992 1518 244
BssSI C'ACGA_G
Cuts at: 0 5525 5754
Size: 5525 229
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3068 5754
Size: 3068 2686
BstBI TT'CG_AA
Cuts at: 0 4358 5754
Size: 4358 1396
BstEII G'GTnAC_C
Cuts at: 0 4203 4707 5754
Size: 4203 504 1047
Fragments arranged by size:
4203 1047 504
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2205 2346 4168 5754
Size: 2205 141 1822 1586
Fragments arranged by size:
2205 1822 1586 141
BstYI r'GATC_y
Cuts at: 0 1425 1755 2843 2978 3267 4688 4946 5738
Size: 1425 330 1088 135 289 1421 258 792
Cuts at: 5738 5754
Size: 16
Fragments arranged by size:
1425 1421 1088 792 330 289 258 135
16
Bsu36I CC'TnA_GG
Cuts at: 0 971 1198 2080 4888 5754
Size: 971 227 882 2808 866
Fragments arranged by size:
2808 971 882 866 227
BtgI C'CryG_G
Cuts at: 0 215 352 5588 5754
Size: 215 137 5236 166
Fragments arranged by size:
5236 215 166 137
BtsI GCAGTG_nn'
Cuts at: 0 382 1091 5754
Size: 382 709 4663
Fragments arranged by size:
4663 709 382
DraIII CAC_nnn'GTG
Cuts at: 0 311 3408 4301 5754
Size: 311 3097 893 1453
Fragments arranged by size:
3097 1453 893 311
EaeI y'GGCC_r
Cuts at: 0 1761 2594 2888 3134 3710 4065 4638 5135
Size: 1761 833 294 246 576 355 573 497
Cuts at: 5135 5222 5553 5711 5754
Size: 87 331 158 43
Fragments arranged by size:
1761 833 576 573 497 355 331 294
246 158 87 43
EarI CTCTTCn'nnn_
Cuts at: 0 194 857 1198 2516 2549 3496 3563 3936
Size: 194 663 341 1318 33 947 67 373
Cuts at: 3936 4916 5005 5171 5483 5754
Size: 980 89 166 312 271
Fragments arranged by size:
1318 980 947 663 373 341 312 271
194 166 89 67 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 396 2098 2559 3871 5754
Size: 396 1702 461 1312 1883
Fragments arranged by size:
1883 1702 1312 461 396
EcoICRI GAG'CTC
Cuts at: 0 516 555 5754
Size: 516 39 5199
Fragments arranged by size:
5199 516 39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 736 1330 1549 1676 1807 1924 2086 2227
Size: 736 594 219 127 131 117 162 141
Cuts at: 2227 2368 2378 2887 3292 5023 5057 5754
Size: 141 10 509 405 1731 34 697
Fragments arranged by size:
1731 736 697 594 509 405 219 162
141 141 131 127 117 34 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 751 886 1596 1865 2986 3436 4334 5754
Size: 751 135 710 269 1121 450 898 1420
Fragments arranged by size:
1420 1121 898 751 710 450 269 135
EcoO109I rG'GnC_Cy
Cuts at: 0 61 756 1049 1194 1855 1902 2138 2401
Size: 61 695 293 145 661 47 236 263
Cuts at: 2401 2534 3769 4995 5091 5754
Size: 133 1235 1226 96 663
Fragments arranged by size:
1235 1226 695 663 661 293 263 236
145 133 96 61 47
EcoRI G'AATT_C
Cuts at: 0 39 1329 2104 5754
Size: 39 1290 775 3650
Fragments arranged by size:
3650 1290 775 39
EcoRV GAT'ATC
Cuts at: 0 3638 5754
Size: 3638 2116
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 535 1395 5250 5473 5754
Size: 535 860 3855 223 281
Fragments arranged by size:
3855 860 535 281 223
FspI TGC'GCA
Cuts at: 0 3502 5754
Size: 3502 2252
HaeII r_GCGC'y
Cuts at: 0 4401 4551 5235 5754
Size: 4401 150 684 519
Fragments arranged by size:
4401 684 519 150
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1427 1459 4835 4867 5427 5459 5754
Size: 1427 32 3376 32 560 32 295
Fragments arranged by size:
3376 1427 560 295 32 32 32
HincII GTy'rAC
Cuts at: 0 855 4234 5754
Size: 855 3379 1520
Fragments arranged by size:
3379 1520 855
HindIII A'AGCT_T
Cuts at: 0 1179 3065 3827 5754
Size: 1179 1886 762 1927
Fragments arranged by size:
1927 1886 1179 762
MfeI C'AATT_G
Cuts at: 0 2010 5754
Size: 2010 3744
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3347 3665 5754
Size: 3347 318 2089
Fragments arranged by size:
3347 2089 318
MscI TGG'CCA
Cuts at: 0 1763 2596 3136 3712 4640 5137 5224 5555
Size: 1763 833 540 576 928 497 87 331
Cuts at: 5555 5754
Size: 199
Fragments arranged by size:
1763 928 833 576 540 497 331 199
87
MslI CAynn'nnrTG
Cuts at: 0 23 961 1040 1255 3652 3703 3923 4166
Size: 23 938 79 215 2397 51 220 243
Cuts at: 4166 4419 4700 5561 5754
Size: 253 281 861 193
Fragments arranged by size:
2397 938 861 281 253 243 220 215
193 79 51 23
MspA1I CmG'CkG
Cuts at: 0 1476 2541 2632 3105 4352 4461 4478 4761
Size: 1476 1065 91 473 1247 109 17 283
Cuts at: 4761 4914 5028 5754
Size: 153 114 726
Fragments arranged by size:
1476 1247 1065 726 473 283 153 114
109 91 17
NcoI C'CATG_G
Cuts at: 0 215 352 5588 5754
Size: 215 137 5236 166
Fragments arranged by size:
5236 215 166 137
NheI G'CTAG_C
Cuts at: 0 1479 5754
Size: 1479 4275
NsiI A_TGCA'T
Cuts at: 0 1366 3955 4438 5168 5754
Size: 1366 2589 483 730 586
Fragments arranged by size:
2589 1366 730 586 483
NspI r_CATG'y
Cuts at: 0 262 1368 3702 3901 5170 5396 5695 5754
Size: 262 1106 2334 199 1269 226 299 59
Fragments arranged by size:
2334 1269 1106 299 262 226 199 59
PciI A'CATG_T
Cuts at: 0 5392 5754
Size: 5392 362
PflMI CCAn_nnn'nTGG
Cuts at: 0 621 1995 2577 3270 3549 4438 5144 5754
Size: 621 1374 582 693 279 889 706 610
Fragments arranged by size:
1374 889 706 693 621 610 582 279
PfoI T'CCnGG_A
Cuts at: 0 752 1553 1565 1574 1860 2903 4013 5754
Size: 752 801 12 9 286 1043 1110 1741
Fragments arranged by size:
1741 1110 1043 801 752 286 12 9
PmlI CAC'GTG
Cuts at: 0 5603 5754
Size: 5603 151
PpuMI rG'GwC_Cy
Cuts at: 0 756 1855 2401 5091 5754
Size: 756 1099 546 2690 663
Fragments arranged by size:
2690 1099 756 663 546
PshAI GACnn'nnGTC
Cuts at: 0 5096 5754
Size: 5096 658
PstI C_TGCA'G
Cuts at: 0 461 917 2249 2739 3635 3865 4774 5754
Size: 461 456 1332 490 896 230 909 980
Fragments arranged by size:
1332 980 909 896 490 461 456 230
PvuII CAG'CTG
Cuts at: 0 1476 2632 3105 4352 4761 5028 5754
Size: 1476 1156 473 1247 409 267 726
Fragments arranged by size:
1476 1247 1156 726 473 409 267
SacI G_AGCT'C
Cuts at: 0 518 557 5754
Size: 518 39 5197
Fragments arranged by size:
5197 518 39
SapI GCTCTTCn'nnn_
Cuts at: 0 3496 5005 5483 5754
Size: 3496 1509 478 271
Fragments arranged by size:
3496 1509 478 271
ScaI AGT'ACT
Cuts at: 0 3427 5754
Size: 3427 2327
SexAI A'CCwGG_T
Cuts at: 0 307 5294 5754
Size: 307 4987 460
Fragments arranged by size:
4987 460 307
SfcI C'TryA_G
Cuts at: 0 333 401 457 913 1542 2245 2735 2812
Size: 333 68 56 456 629 703 490 77
Cuts at: 2812 2983 3631 3861 4770 5364 5754
Size: 171 648 230 909 594 390
Fragments arranged by size:
909 703 648 629 594 490 456 390
333 230 171 77 68 56
SgrAI Cr'CCGG_yG
Cuts at: 0 3992 5754
Size: 3992 1762
SmlI C'TyrA_G
Cuts at: 0 550 3034 3151 3292 4216 4990 5235 5383
Size: 550 2484 117 141 924 774 245 148
Cuts at: 5383 5754
Size: 371
Fragments arranged by size:
2484 924 774 550 371 245 148 141
117
SphI G_CATG'C
Cuts at: 0 262 5695 5754
Size: 262 5433 59
Fragments arranged by size:
5433 262 59
SspI AAT'ATT
Cuts at: 0 4718 5754
Size: 4718 1036
StuI AGG'CCT
Cuts at: 0 1540 4262 5754
Size: 1540 2722 1492
Fragments arranged by size:
2722 1540 1492
StyI C'CwwG_G
Cuts at: 0 160 215 352 1370 1636 3561 3713 5588
Size: 160 55 137 1018 266 1925 152 1875
Cuts at: 5588 5754
Size: 166
Fragments arranged by size:
1925 1875 1018 266 166 160 152 137
55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2029 4178 4726 5419 5754
Size: 2029 2149 548 693 335
Fragments arranged by size:
2149 2029 693 548 335
TatI w'GTAC_w
Cuts at: 0 618 2252 3425 3683 4618 5754
Size: 618 1634 1173 258 935 1136
Fragments arranged by size:
1634 1173 1136 935 618 258
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2040 2072 5754
Size: 2040 32 3682
Fragments arranged by size:
3682 2040 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2617 4648 4720 5754
Size: 2617 2031 72 1034
Fragments arranged by size:
2617 2031 1034 72
XmnI GAAnn'nnTTC
Cuts at: 0 452 1586 2071 5754
Size: 452 1134 485 3683
Fragments arranged by size:
3683 1134 485 452
Enzymes that do cut:
AccI AcuI AfeI AflII AflIII AgeI AleI AlfI
AloI AlwNI ApaLI ApoI AseI AvaI AvrII BaeI
BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtsI DraIII EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII MfeI MmeI MscI MslI
MspA1I NcoI NheI NsiI NspI PciI PflMI PfoI
PmlI PpuMI PshAI PstI PvuII SacI SapI ScaI
SexAI SfcI SgrAI SmlI SphI SspI StuI StyI
TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AhdI ApaI AscI AsiSI
BamHI BbeI BsiEI BsiWI BsmBI BspEI BsrBI BsrGI
BssHII BstZ17I BtgZI ClaI CspCI DraI DrdI EagI
FseI FspAI HpaI KasI KpnI MluI NaeI NarI
NdeI NgoMIV NotI NruI PacI PasI PmeI PpiI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SbfI SfiI SfoI SgfI SmaI SnaBI SpeI
SrfI SwaI Tth111I XbaI XhoI XmaI ZraI