Restriction map for
pF1KSDA1410
(Linear) (Six-base) MAPSORT of: KIAA1410.seq Check: 5232 from: 1 to: 12795
KIAA1410.orf
With 182 enzymes: SgfI *
April 11, 2008 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 516 1419 2366 4064 7605 12096 12795
Size: 516 903 947 1698 3541 4491 699
Fragments arranged by size:
4491 3541 1698 947 903 699 516
AatII G_ACGT'C
Cuts at: 0 5641 11972 12795
Size: 5641 6331 823
Fragments arranged by size:
6331 5641 823
AccI GT'mk_AC
Cuts at: 0 3508 8008 12795
Size: 3508 4500 4787
Fragments arranged by size:
4787 4500 3508
Acc65I G'GTAC_C
Cuts at: 0 6570 12795
Size: 6570 6225
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 754 913 1030 1049 2977 2991 3580
Size: 446 308 159 117 19 1928 14 589
Cuts at: 3580 3700 5110 6109 6764 7687 8269 9340 9940
Size: 120 1410 999 655 923 582 1071 600
Cuts at: 9940 9942 10180 10501 11401 12795
Size: 2 238 321 900 1394
Fragments arranged by size:
1928 1410 1394 1071 999 923 900 655
600 589 582 446 321 308 238 159
120 117 19 14 2
AfeI AGC'GCT
Cuts at: 0 3796 4472 7483 9685 12795
Size: 3796 676 3011 2202 3110
Fragments arranged by size:
3796 3110 3011 2202 676
AflIII A'CryG_T
Cuts at: 0 1119 4118 5834 12795
Size: 1119 2999 1716 6961
Fragments arranged by size:
6961 2999 1716 1119
AhdI GACnn_n'nnGTC
Cuts at: 0 7554 9645 10695 12795
Size: 7554 2091 1050 2100
Fragments arranged by size:
7554 2100 2091 1050
AleI CACnn'nnGTG
Cuts at: 0 1498 2479 4042 8920 12795
Size: 1498 981 1563 4878 3875
Fragments arranged by size:
4878 3875 1563 1498 981
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1732 5092 8968 11870 12407 12795
Size: 1732 3360 3876 2902 537 388
Fragments arranged by size:
3876 3360 2902 1732 537 388
AlwNI CAG_nnn'CTG
Cuts at: 0 253 543 597 621 891 1230 2319 2448
Size: 253 290 54 24 270 339 1089 129
Cuts at: 2448 2744 2973 3091 3300 3483 3588 3777 6386
Size: 296 229 118 209 183 105 189 2609
Cuts at: 6386 7281 7392 7734 9345 10071 10158 10182 10872
Size: 895 111 342 1611 726 87 24 690
Cuts at: 10872 11047 12123 12795
Size: 175 1076 672
Fragments arranged by size:
2609 1611 1089 1076 895 726 690 672
342 339 296 290 270 253 229 209
189 183 175 129 118 111 105 87
54 24 24
ApaI G_GGCC'C
Cuts at: 0 52 1254 3258 5588 7033 7206 9487 11131
Size: 52 1202 2004 2330 1445 173 2281 1644
Cuts at: 11131 11507 12795
Size: 376 1288
Fragments arranged by size:
2330 2281 2004 1644 1445 1288 1202 376
173 52
ApaLI G'TGCA_C
Cuts at: 0 2008 2912 4225 6914 12795
Size: 2008 904 1313 2689 5881
Fragments arranged by size:
5881 2689 2008 1313 904
ApoI r'AATT_y
Cuts at: 0 1621 2689 8655 10786 12387 12400 12795
Size: 1621 1068 5966 2131 1601 13 395
Fragments arranged by size:
5966 2131 1621 1601 1068 395 13
AvaI C'yCGr_G
Cuts at: 0 111 828 1384 1590 5052 7035 8835 8964
Size: 111 717 556 206 3462 1983 1800 129
Cuts at: 8964 10003 10446 11132 11745 12795
Size: 1039 443 686 613 1050
Fragments arranged by size:
3462 1983 1800 1050 1039 717 686 613
556 443 206 129 111
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 577 610 4135 4168 6554 6587 7387 7420
Size: 577 33 3525 33 2386 33 800 33
Cuts at: 7420 8193 8226 12795
Size: 773 33 4569
Fragments arranged by size:
4569 3525 2386 800 773 577 33 33
33 33 33
BamHI G'GATC_C
Cuts at: 0 1073 3320 9215 12308 12528 12795
Size: 1073 2247 5895 3093 220 267
Fragments arranged by size:
5895 3093 2247 1073 267 220
BanI G'GyrC_C
Cuts at: 0 528 662 1065 1431 4319 4729 5019 6346
Size: 528 134 403 366 2888 410 290 1327
Cuts at: 6346 6484 6570 6985 8895 8914 10683 11202 11899
Size: 138 86 415 1910 19 1769 519 697
Cuts at: 11899 11994 12453 12517 12795
Size: 95 459 64 278
Fragments arranged by size:
2888 1910 1769 1327 697 528 519 459
415 410 403 366 290 278 138 134
95 86 64 19
BanII G_rGCy'C
Cuts at: 0 52 518 1254 3258 3568 3746 4710 5588
Size: 52 466 736 2004 310 178 964 878
Cuts at: 5588 6029 6044 6314 7033 7206 7232 7589 8152
Size: 441 15 270 719 173 26 357 563
Cuts at: 8152 8175 9164 9487 9572 10657 10817 11131 11507
Size: 23 989 323 85 1085 160 314 376
Cuts at: 11507 12221 12795
Size: 714 574
Fragments arranged by size:
2004 1085 989 964 878 736 719 714
574 563 466 441 376 357 323 314
310 270 178 173 160 85 52 26
23 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 434 5213 6703 7329 8157 11172 11411 12324
Size: 434 4779 1490 626 828 3015 239 913
Cuts at: 12324 12795
Size: 471
Fragments arranged by size:
4779 3015 1490 913 828 626 471 434
239
BbvCI CC'TCA_GC
Cuts at: 0 304 1094 1300 1537 2704 3446 3940 4567
Size: 304 790 206 237 1167 742 494 627
Cuts at: 4567 5911 6067 6552 12795
Size: 1344 156 485 6243
Fragments arranged by size:
6243 1344 1167 790 742 627 494 485
304 237 206 156
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4050 4084 4899 4933 7446 7480 8797 8831
Size: 4050 34 815 34 2513 34 1317 34
Cuts at: 8831 11113 11147 12795
Size: 2282 34 1648
Fragments arranged by size:
4050 2513 2282 1648 1317 815 34 34
34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 8622 12795
Size: 8622 4173
BclI T'GATC_A
Cuts at: 0 3860 4664 5138 9023 12110 12795
Size: 3860 804 474 3885 3087 685
Fragments arranged by size:
3885 3860 3087 804 685 474
BfrBI ATG'CAT
Cuts at: 0 9293 12795
Size: 9293 3502
BglI GCCn_nnn'nGGC
Cuts at: 0 4252 4748 8701 9168 9356 12795
Size: 4252 496 3953 467 188 3439
Fragments arranged by size:
4252 3953 3439 496 467 188
BglII A'GATC_T
Cuts at: 0 2558 4013 5147 10007 11544 11834 12795
Size: 2558 1455 1134 4860 1537 290 961
Fragments arranged by size:
4860 2558 1537 1455 1134 961 290
BlpI GC'TnA_GC
Cuts at: 0 2594 3149 5233 10192 12795
Size: 2594 555 2084 4959 2603
Fragments arranged by size:
4959 2603 2594 2084 555
Bme1580I G_kGCm'C
Cuts at: 0 52 1254 2012 2093 2916 3258 4229 4734
Size: 52 1202 758 81 823 342 971 505
Cuts at: 4734 5372 5588 6605 6918 7033 7206 9487 11131
Size: 638 216 1017 313 115 173 2281 1644
Cuts at: 11131 11507 11902 12522 12649 12786 12795
Size: 376 395 620 127 137 9
Fragments arranged by size:
2281 1644 1202 1017 971 823 758 638
620 505 395 376 342 313 216 173
137 127 115 81 52 9
BmgBI CAC'GTC
Cuts at: 0 4503 11715 12795
Size: 4503 7212 1080
Fragments arranged by size:
7212 4503 1080
BmrI ACTGGGnnnn_n'
Cuts at: 0 1071 1770 3339 4272 6362 8943 10043 10081
Size: 1071 699 1569 933 2090 2581 1100 38
Cuts at: 10081 11305 11673 12031 12795
Size: 1224 368 358 764
Fragments arranged by size:
2581 2090 1569 1224 1100 1071 933 764
699 368 358 38
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1280 1410 6277 12795
Size: 1280 130 4867 6518
Fragments arranged by size:
6518 4867 1280 130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 1636 2047 2119 2167 2284 2662 3024
Size: 140 1496 411 72 48 117 378 362
Cuts at: 3024 3178 3421 3721 3760 3973 4126 4155 4378
Size: 154 243 300 39 213 153 29 223
Cuts at: 4378 6043 6154 6357 6484 6922 7274 8488 8578
Size: 1665 111 203 127 438 352 1214 90
Cuts at: 8578 8599 8921 9202 9334 9856 9889 10126 10963
Size: 21 322 281 132 522 33 237 837
Cuts at: 10963 11506 12196 12243 12795
Size: 543 690 47 552
Fragments arranged by size:
1665 1496 1214 837 690 552 543 522
438 411 378 362 352 322 300 281
243 237 223 213 203 154 153 140
132 127 117 111 90 72 48 47
39 33 29 21
Bpu10I CC'TnA_GC
Cuts at: 0 304 598 1094 1231 1255 1300 1436 1537
Size: 304 294 496 137 24 45 136 101
Cuts at: 1537 2704 3446 3940 4567 5911 6067 6391 6552
Size: 1167 742 494 627 1344 156 324 161
Cuts at: 6552 9538 11726 11999 12215 12795
Size: 2986 2188 273 216 580
Fragments arranged by size:
2986 2188 1344 1167 742 627 580 496
494 324 304 294 273 216 161 156
137 136 101 45 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1654 4702 5287 5377 5488 6199 6247 6382
Size: 1654 3048 585 90 111 711 48 135
Cuts at: 6382 6709 7462 7873 8998 9868 10441 10831 11554
Size: 327 753 411 1125 870 573 390 723
Cuts at: 11554 11599 11974 12121 12130 12232 12795
Size: 45 375 147 9 102 563
Fragments arranged by size:
3048 1654 1125 870 753 723 711 585
573 563 411 390 375 327 147 135
111 102 90 48 45 9
BsaI GGTCTCn'nnnn_
Cuts at: 0 812 1381 1747 3949 4759 5542 6898 8641
Size: 812 569 366 2202 810 783 1356 1743
Cuts at: 8641 12795
Size: 4154
Fragments arranged by size:
4154 2202 1743 1356 812 810 783 569
366
BsaAI yAC'GTr
Cuts at: 0 2911 5697 8815 12795
Size: 2911 2786 3118 3980
Fragments arranged by size:
3980 3118 2911 2786
BsaBI GATnn'nnATC
Cuts at: 0 571 3355 9967 11543 12795
Size: 571 2784 6612 1576 1252
Fragments arranged by size:
6612 2784 1576 1252 571
BsaHI Gr'CG_yC
Cuts at: 0 917 5638 7688 11969 12795
Size: 917 4721 2050 4281 826
Fragments arranged by size:
4721 4281 2050 917 826
BsaWI w'CCGG_w
Cuts at: 0 4702 10686 12795
Size: 4702 5984 2109
Fragments arranged by size:
5984 4702 2109
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 439 469 790 820 2272 2302 3022 3052
Size: 439 30 321 30 1452 30 720 30
Cuts at: 3052 3325 3355 3487 3517 4132 4162 6330 6360
Size: 273 30 132 30 615 30 2168 30
Cuts at: 6360 9307 9337 9424 9454 10399 10429 10987 11017
Size: 2947 30 87 30 945 30 558 30
Cuts at: 11017 11428 11458 11704 11734 12409 12439 12795
Size: 411 30 246 30 675 30 356
Fragments arranged by size:
2947 2168 1452 945 720 675 615 558
439 411 356 321 273 246 132 87
30 30 30 30 30 30 30 30
30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 594 949 1435 1438 2548 2599 2659 2818
Size: 594 355 486 3 1110 51 60 159
Cuts at: 2818 3280 3718 3978 4153 5344 5455 5740 5772
Size: 462 438 260 175 1191 111 285 32
Cuts at: 5772 6115 6202 6523 6540 6712 7462 7465 7639
Size: 343 87 321 17 172 750 3 174
Cuts at: 7639 8596 8659 8686 9322 9865 10105 10123 10188
Size: 957 63 27 636 543 240 18 65
Cuts at: 10188 11968 12795
Size: 1780 827
Fragments arranged by size:
1780 1191 1110 957 827 750 636 594
543 486 462 438 355 343 321 285
260 240 175 174 172 159 111 87
65 63 60 51 32 27 18 17
3 3
BseYI C'CCAG_C
Cuts at: 0 2093 2392 4315 4391 4734 4743 4876 5293
Size: 2093 299 1923 76 343 9 133 417
Cuts at: 5293 5317 6028 7274 7734 7752 9345 9524 9588
Size: 24 711 1246 460 18 1593 179 64
Cuts at: 9588 9680 9861 9924 11296 12324 12524 12743 12795
Size: 92 181 63 1372 1028 200 219 52
Fragments arranged by size:
2093 1923 1593 1372 1246 1028 711 460
417 343 299 219 200 181 179 133
92 76 64 63 52 24 18 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 1678 1864 1890 2800 3949 4066 4696
Size: 78 1600 186 26 910 1149 117 630
Cuts at: 4696 6640 7405 8683 12795
Size: 1944 765 1278 4112
Fragments arranged by size:
4112 1944 1600 1278 1149 910 765 630
186 117 78 26
BsiEI CG_ry'CG
Cuts at: 0 4043 6972 7773 9613 12795
Size: 4043 2929 801 1840 3182
Fragments arranged by size:
4043 3182 2929 1840 801
BsiHKAI G_wGCw'C
Cuts at: 0 69 1261 1298 1901 2012 2351 2916 4229
Size: 69 1192 37 603 111 339 565 1313
Cuts at: 4229 4710 5702 6918 7589 8175 9515 11243 11720
Size: 481 992 1216 671 586 1340 1728 477
Cuts at: 11720 11732 12221 12795
Size: 12 489 574
Fragments arranged by size:
1728 1340 1313 1216 1192 992 671 603
586 574 565 489 481 477 339 111
69 37 12
BsmI GAATG_Cn'
Cuts at: 0 1017 3843 3890 4617 9295 12795
Size: 1017 2826 47 727 4678 3500
Fragments arranged by size:
4678 3500 2826 1017 727 47
BsmBI CGTCTCn'nnnn_
Cuts at: 0 9397 11875 12795
Size: 9397 2478 920
Fragments arranged by size:
9397 2478 920
Bsp1286I G_dGCh'C
Cuts at: 0 52 69 518 1254 1261 1298 1901 2012
Size: 52 17 449 736 7 37 603 111
Cuts at: 2012 2093 2351 2916 3258 3568 3746 4229 4710
Size: 81 258 565 342 310 178 483 481
Cuts at: 4710 4734 5372 5588 5702 6029 6044 6314 6605
Size: 24 638 216 114 327 15 270 291
Cuts at: 6605 6918 7033 7206 7232 7589 8152 8175 9164
Size: 313 115 173 26 357 563 23 989
Cuts at: 9164 9487 9515 9572 10657 10817 11131 11243 11507
Size: 323 28 57 1085 160 314 112 264
Cuts at: 11507 11720 11732 11902 12221 12522 12649 12786 12795
Size: 213 12 170 319 301 127 137 9
Fragments arranged by size:
1085 989 736 638 603 565 563 483
481 449 357 342 327 323 319 314
313 310 301 291 270 264 258 216
213 178 173 170 160 137 127 115
114 112 111 81 57 52 37 28
26 24 23 17 15 12 9 7
BspHI T'CATG_A
Cuts at: 0 2163 3605 3830 10598 10667 11681 12795
Size: 2163 1442 225 6768 69 1014 1114
Fragments arranged by size:
6768 2163 1442 1114 1014 225 69
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 516 1419 2366 2513 3419 4064 6513 7605
Size: 516 903 947 147 906 645 2449 1092
Cuts at: 7605 9425 10745 11384 12096 12284 12795
Size: 1820 1320 639 712 188 511
Fragments arranged by size:
2449 1820 1320 1092 947 906 903 712
645 639 516 511 188 147
BsrBI CCG'CTC
Cuts at: 0 2649 3813 8501 12795
Size: 2649 1164 4688 4294
Fragments arranged by size:
4688 4294 2649 1164
BsrDI GCAATG_nn'
Cuts at: 0 2195 2282 5951 7211 7746 9074 12022 12713
Size: 2195 87 3669 1260 535 1328 2948 691
Cuts at: 12713 12795
Size: 82
Fragments arranged by size:
3669 2948 2195 1328 1260 691 535 87
82
BsrFI r'CCGG_y
Cuts at: 0 4363 5847 6894 8235 10187 11669 12795
Size: 4363 1484 1047 1341 1952 1482 1126
Fragments arranged by size:
4363 1952 1484 1482 1341 1126 1047
BsrGI T'GTAC_A
Cuts at: 0 8336 9965 12197 12572 12795
Size: 8336 1629 2232 375 223
Fragments arranged by size:
8336 2232 1629 375 223
BssHII G'CGCG_C
Cuts at: 0 9120 12795
Size: 9120 3675
BssSI C'ACGA_G
Cuts at: 0 2917 7408 12668 12795
Size: 2917 4491 5260 127
Fragments arranged by size:
5260 4491 2917 127
BstAPI GCAn_nnn'nTGC
Cuts at: 0 7392 8574 9177 9426 10538 12795
Size: 7392 1182 603 249 1112 2257
Fragments arranged by size:
7392 2257 1182 1112 603 249
BstEII G'GTnAC_C
Cuts at: 0 1020 1566 2134 4305 7503 10905 12795
Size: 1020 546 568 2171 3198 3402 1890
Fragments arranged by size:
3402 3198 2171 1890 1020 568 546
BstXI CCAn_nnnn'nTGG
Cuts at: 0 666 2898 4179 4344 4503 4779 5187 5721
Size: 666 2232 1281 165 159 276 408 534
Cuts at: 5721 6036 7143 7251 8094 9156 10941 11112 12795
Size: 315 1107 108 843 1062 1785 171 1683
Fragments arranged by size:
2232 1785 1683 1281 1107 1062 843 666
534 408 315 276 171 165 159 108
BstYI r'GATC_y
Cuts at: 0 1073 1631 2372 2558 2756 3320 3815 4013
Size: 1073 558 741 186 198 564 495 198
Cuts at: 4013 4556 5009 5147 8279 9215 9605 10007 10178
Size: 543 453 138 3132 936 390 402 171
Cuts at: 10178 10271 10361 11544 11762 11834 12308 12528 12725
Size: 93 90 1183 218 72 474 220 197
Cuts at: 12725 12795
Size: 70
Fragments arranged by size:
3132 1183 1073 936 741 564 558 543
495 474 453 402 390 220 218 198
198 197 186 171 138 93 90 72
70
BstZ17I GTA'TAC
Cuts at: 0 8009 12795
Size: 8009 4786
Bsu36I CC'TnA_GG
Cuts at: 0 44 7318 12795
Size: 44 7274 5477
Fragments arranged by size:
7274 5477 44
BtgI C'CryG_G
Cuts at: 0 229 1069 2153 3806 4037 4337 4844 5108
Size: 229 840 1084 1653 231 300 507 264
Cuts at: 5108 5552 6566 6608 6954 7025 7063 8609 9155
Size: 444 1014 42 346 71 38 1546 546
Cuts at: 9155 10343 11472 11784 12498 12795
Size: 1188 1129 312 714 297
Fragments arranged by size:
1653 1546 1188 1129 1084 1014 840 714
546 507 444 346 312 300 297 264
231 229 71 42 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 4062 4296 8622 9246 9461 10437 12227 12795
Size: 4062 234 4326 624 215 976 1790 568
Fragments arranged by size:
4326 4062 1790 976 624 568 234 215
BtsI GCAGTG_nn'
Cuts at: 0 69 460 499 1159 1252 1828 2227 4466
Size: 69 391 39 660 93 576 399 2239
Cuts at: 4466 5039 6369 7501 9337 12121 12795
Size: 573 1330 1132 1836 2784 674
Fragments arranged by size:
2784 2239 1836 1330 1132 674 660 576
573 399 391 93 69 39
ClaI AT'CG_AT
Cuts at: 0 3143 6878 9047 12795
Size: 3143 3735 2169 3748
Fragments arranged by size:
3748 3735 3143 2169
DraIII CAC_nnn'GTG
Cuts at: 0 1120 8136 9144 11362 11430 12795
Size: 1120 7016 1008 2218 68 1365
Fragments arranged by size:
7016 2218 1365 1120 1008 68
DrdI GACnn_nn'nnGTC
Cuts at: 0 1369 1575 2677 3087 3601 8032 9526 12795
Size: 1369 206 1102 410 514 4431 1494 3269
Fragments arranged by size:
4431 3269 1494 1369 1102 514 410 206
EaeI y'GGCC_r
Cuts at: 0 1203 1516 1700 2075 2471 2975 4040 4299
Size: 1203 313 184 375 396 504 1065 259
Cuts at: 4299 4793 4841 4868 5064 5111 6366 7364 7658
Size: 494 48 27 196 47 1255 998 294
Cuts at: 7658 7770 7805 8233 8258 8633 8771 8827 9146
Size: 112 35 428 25 375 138 56 319
Cuts at: 9146 9458 10346 10388 10931 12026 12584 12795
Size: 312 888 42 543 1095 558 211
Fragments arranged by size:
1255 1203 1095 1065 998 888 558 543
504 494 428 396 375 375 319 313
312 294 259 211 196 184 138 112
56 48 47 42 35 27 25
EagI C'GGCC_G
Cuts at: 0 4040 7770 12795
Size: 4040 3730 5025
Fragments arranged by size:
5025 4040 3730
EarI CTCTTCn'nnn_
Cuts at: 0 1090 1380 2062 2174 3556 5104 5404 6431
Size: 1090 290 682 112 1382 1548 300 1027
Cuts at: 6431 6500 7684 7930 8491 8992 9050 10372 10764
Size: 69 1184 246 561 501 58 1322 392
Cuts at: 10764 12795
Size: 2031
Fragments arranged by size:
2031 1548 1382 1322 1184 1090 1027 682
561 501 392 300 290 246 112 69
58
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 6843 11263 12795
Size: 6843 4420 1532
Fragments arranged by size:
6843 4420 1532
EcoICRI GAG'CTC
Cuts at: 0 4708 7587 8173 12219 12795
Size: 4708 2879 586 4046 576
Fragments arranged by size:
4708 4046 2879 586 576
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 446 754 913 1030 1049 1636 2047
Size: 140 306 308 159 117 19 587 411
Cuts at: 2047 2119 2167 2284 2662 2977 2991 3024 3178
Size: 72 48 117 378 315 14 33 154
Cuts at: 3178 3421 3580 3700 3721 3760 3973 4126 4155
Size: 243 159 120 21 39 213 153 29
Cuts at: 4155 4378 5110 6043 6109 6154 6357 6484 6764
Size: 223 732 933 66 45 203 127 280
Cuts at: 6764 6922 7274 7687 8269 8488 8578 8599 8921
Size: 158 352 413 582 219 90 21 322
Cuts at: 8921 9202 9334 9340 9856 9889 9940 9942 10126
Size: 281 132 6 516 33 51 2 184
Cuts at: 10126 10180 10501 10963 11401 11506 12196 12243 12795
Size: 54 321 462 438 105 690 47 552
Fragments arranged by size:
933 732 690 587 582 552 516 462
438 413 411 378 352 322 321 315
308 306 281 280 243 223 219 213
203 184 159 159 158 154 153 140
132 127 120 117 117 105 90 72
66 54 51 48 47 45 39 33
33 29 21 21 19 14 6 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 373 1439 1540 1843 2509 4783 5383 5467
Size: 373 1066 101 303 666 2274 600 84
Cuts at: 5467 7330 7726 7888 8578 8740 9181 9223 9244
Size: 1863 396 162 690 162 441 42 21
Cuts at: 9244 10108 11116 12136 12795
Size: 864 1008 1020 659
Fragments arranged by size:
2274 1863 1066 1020 1008 864 690 666
659 600 441 396 373 303 162 162
101 84 42 21
EcoO109I rG'GnC_Cy
Cuts at: 0 38 48 86 96 873 1098 1251 1279
Size: 38 10 38 10 777 225 153 28
Cuts at: 1279 1955 2316 2466 3936 4032 4101 4253 4274
Size: 676 361 150 1470 96 69 152 21
Cuts at: 4274 4863 4880 5388 6114 6258 7030 7202 7203
Size: 589 17 508 726 144 772 172 1
Cuts at: 7203 7323 7881 8067 8553 8733 8961 9484 11127
Size: 120 558 186 486 180 228 523 1643
Cuts at: 11127 11128 11504 11610 12795
Size: 1 376 106 1185
Fragments arranged by size:
1643 1470 1185 777 772 726 676 589
558 523 508 486 376 361 228 225
186 180 172 153 152 150 144 120
106 96 69 38 38 28 21 17
10 10 1 1
EcoRI G'AATT_C
Cuts at: 0 2689 8655 10786 12795
Size: 2689 5966 2131 2009
Fragments arranged by size:
5966 2689 2131 2009
EcoRV GAT'ATC
Cuts at: 0 7968 12795
Size: 7968 4827
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1374 1485 1506 3660 5115 6135 8436 9156
Size: 1374 111 21 2154 1455 1020 2301 720
Cuts at: 9156 12795
Size: 3639
Fragments arranged by size:
3639 2301 2154 1455 1374 1020 720 111
21
FspI TGC'GCA
Cuts at: 0 1144 1273 1708 1789 3304 4060 5031 8119
Size: 1144 129 435 81 1515 756 971 3088
Cuts at: 8119 8521 8782 9793 11152 11551 12795
Size: 402 261 1011 1359 399 1244
Fragments arranged by size:
3088 1515 1359 1244 1144 1011 971 756
435 402 399 261 129 81
HaeII r_GCGC'y
Cuts at: 0 3798 3971 4474 5098 7485 7735 9240 9687
Size: 3798 173 503 624 2387 250 1505 447
Cuts at: 9687 11493 11676 11744 12795
Size: 1806 183 68 1051
Fragments arranged by size:
3798 2387 1806 1505 1051 624 503 447
250 183 173 68
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 767 790 799 822 2272 2304 2817 2849
Size: 767 23 9 23 1450 32 513 32
Cuts at: 2849 3022 3054 3118 3150 3325 3357 3375 3407
Size: 173 32 64 32 175 32 18 32
Cuts at: 3407 3562 3594 4003 4035 4692 4724 4912 4944
Size: 155 32 409 32 657 32 188 32
Cuts at: 4944 5555 5587 7540 7561 7572 7593 9631 9663
Size: 611 32 1953 21 11 21 2038 32
Cuts at: 9663 10662 10681 10694 10713 10959 10987 10991 11019
Size: 999 19 13 19 246 28 4 28
Cuts at: 11019 11428 11460 11704 11736 12153 12185 12409 12441
Size: 409 32 244 32 417 32 224 32
Cuts at: 12441 12442 12474 12651 12683 12795
Size: 1 32 177 32 112
Fragments arranged by size:
2038 1953 1450 999 767 657 611 513
417 409 409 246 244 224 188 177
175 173 155 112 64 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 28
28 23 23 21 21 19 19 18
13 11 9 4 1
HincII GTy'rAC
Cuts at: 0 3509 5688 8202 12467 12795
Size: 3509 2179 2514 4265 328
Fragments arranged by size:
4265 3509 2514 2179 328
HindIII A'AGCT_T
Cuts at: 0 411 12795
Size: 411 12384
HpaI GTT'AAC
Cuts at: 0 12467 12795
Size: 12467 328
KpnI G_GTAC'C
Cuts at: 0 6574 12795
Size: 6574 6221
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 320 387 668 3084 3089 3368 3899 5127
Size: 320 67 281 2416 5 279 531 1228
Cuts at: 5127 5441 5837 7136 9359 10406 10520 10787 11024
Size: 314 396 1299 2223 1047 114 267 237
Cuts at: 11024 12795
Size: 1771
Fragments arranged by size:
2416 2223 1771 1299 1228 1047 531 396
320 314 281 279 267 237 114 67
5
MscI TGG'CCA
Cuts at: 0 1702 2077 2473 2977 4301 4795 4843 4870
Size: 1702 375 396 504 1324 494 48 27
Cuts at: 4870 5113 8260 8773 9148 10348 10390 10933 12028
Size: 243 3147 513 375 1200 42 543 1095
Cuts at: 12028 12795
Size: 767
Fragments arranged by size:
3147 1702 1324 1200 1095 767 543 513
504 494 396 375 375 243 48 42
27
MslI CAynn'nnrTG
Cuts at: 0 1498 2479 2896 3604 4042 4117 4342 4843
Size: 1498 981 417 708 438 75 225 501
Cuts at: 4843 4921 5719 6598 7249 7252 7351 8131 8399
Size: 78 798 879 651 3 99 780 268
Cuts at: 8399 8920 10237 10342 10600 11023 11852 12580 12718
Size: 521 1317 105 258 423 829 728 138
Cuts at: 12718 12795
Size: 77
Fragments arranged by size:
1498 1317 981 879 829 798 780 728
708 651 521 501 438 423 417 268
258 225 138 105 99 78 77 75
3
MspA1I CmG'CkG
Cuts at: 0 270 1679 2097 2132 2871 2955 2973 3156
Size: 270 1409 418 35 739 84 18 183
Cuts at: 3156 3585 3723 3777 4455 4566 4738 4747 4977
Size: 429 138 54 678 111 172 9 230
Cuts at: 4977 5629 5730 6005 6417 7065 7278 7389 7460
Size: 652 101 275 412 648 213 111 71
Cuts at: 7460 7769 8826 9342 9463 9524 10125 10886 11913
Size: 309 1057 516 121 61 601 761 1027
Cuts at: 11913 12174 12328 12524 12546 12795
Size: 261 154 196 22 249
Fragments arranged by size:
1409 1057 1027 761 739 678 652 648
601 516 429 418 412 309 275 270
261 249 230 213 196 183 172 154
138 121 111 111 101 84 71 61
54 35 22 18 9
NaeI GCC'GGC
Cuts at: 0 4365 6896 8237 12795
Size: 4365 2531 1341 4558
Fragments arranged by size:
4558 4365 2531 1341
NcoI C'CATG_G
Cuts at: 0 3806 4337 4844 6566 6608 7025 8609 11472
Size: 3806 531 507 1722 42 417 1584 2863
Cuts at: 11472 12498 12795
Size: 1026 297
Fragments arranged by size:
3806 2863 1722 1584 1026 531 507 417
297 42
NgoMIV G'CCGG_C
Cuts at: 0 4363 6894 8235 12795
Size: 4363 2531 1341 4560
Fragments arranged by size:
4560 4363 2531 1341
NotI GC'GGCC_GC
Cuts at: 0 7770 12795
Size: 7770 5025
NsiI A_TGCA'T
Cuts at: 0 9295 12795
Size: 9295 3500
NspI r_CATG'y
Cuts at: 0 1110 1194 1213 1818 2794 3222 3318 4122
Size: 1110 84 19 605 976 428 96 804
Cuts at: 4122 4452 5676 5838 6645 6894 7350 7854 9429
Size: 330 1224 162 807 249 456 504 1575
Cuts at: 9429 12795
Size: 3366
Fragments arranged by size:
3366 1575 1224 1110 976 807 804 605
504 456 428 330 249 162 96 84
19
PacI TTA_AT'TAA
Cuts at: 0 1940 12795
Size: 1940 10855
PasI CC'CwG_GG
Cuts at: 0 11112 11508 12795
Size: 11112 396 1287
Fragments arranged by size:
11112 1287 396
PciI A'CATG_T
Cuts at: 0 4118 5834 12795
Size: 4118 1716 6961
Fragments arranged by size:
6961 4118 1716
PflMI CCAn_nnn'nTGG
Cuts at: 0 779 2682 2744 2829 2973 3237 3807 6636
Size: 779 1903 62 85 144 264 570 2829
Cuts at: 6636 6948 7734 8219 9144 9725 10071 10083 12795
Size: 312 786 485 925 581 346 12 2712
Fragments arranged by size:
2829 2712 1903 925 786 779 581 570
485 346 312 264 144 85 62 12
PfoI T'CCnGG_A
Cuts at: 0 126 434 920 2375 2621 3040 4094 4520
Size: 126 308 486 1455 246 419 1054 426
Cuts at: 4520 5462 6131 7427 7958 8057 9218 9833 11579
Size: 942 669 1296 531 99 1161 615 1746
Cuts at: 11579 12509 12795
Size: 930 286
Fragments arranged by size:
1746 1455 1296 1161 1054 942 930 669
615 531 486 426 419 308 286 246
126 99
PmlI CAC'GTG
Cuts at: 0 2911 12795
Size: 2911 9884
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2794 2826 6301 6333 12795
Size: 2794 32 3475 32 6462
Fragments arranged by size:
6462 3475 2794 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 38 86 873 1098 4032 4101 4274 5388
Size: 38 48 787 225 2934 69 173 1114
Cuts at: 5388 7323 7881 8553 8733 8961 11610 12795
Size: 1935 558 672 180 228 2649 1185
Fragments arranged by size:
2934 2649 1935 1185 1114 787 672 558
228 225 180 173 69 48 38
PspOMI G'GGCC_C
Cuts at: 0 48 1250 3254 5584 7029 7202 9483 11127
Size: 48 1202 2004 2330 1445 173 2281 1644
Cuts at: 11127 11503 12795
Size: 376 1292
Fragments arranged by size:
2330 2281 2004 1644 1445 1292 1202 376
173 48
PspXI vC'TCGA_Gb
Cuts at: 0 1590 12795
Size: 1590 11205
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 5041 5073 9406 9438 12795
Size: 5041 32 4333 32 3357
Fragments arranged by size:
5041 4333 3357 32 32
PstI C_TGCA'G
Cuts at: 0 75 506 1823 2234 2870 3416 5384 5491
Size: 75 431 1317 411 636 546 1968 107
Cuts at: 5491 8960 9422 11327 11937 12107 12302 12386 12795
Size: 3469 462 1905 610 170 195 84 409
Fragments arranged by size:
3469 1968 1905 1317 636 610 546 462
431 411 409 195 170 107 84 75
PvuI CG_AT'CG
Cuts at: 0 9613 12795
Size: 9613 3182
PvuII CAG'CTG
Cuts at: 0 1679 2097 2871 2955 2973 3156 3585 3777
Size: 1679 418 774 84 18 183 429 192
Cuts at: 3777 4566 4738 4747 5629 5730 6417 7278 7389
Size: 789 172 9 882 101 687 861 111
Cuts at: 7389 7460 9524 10125 11913 12174 12328 12546 12795
Size: 71 2064 601 1788 261 154 218 249
Fragments arranged by size:
2064 1788 1679 882 861 789 774 687
601 429 418 261 249 218 192 183
172 154 111 101 84 71 18 9
SacI G_AGCT'C
Cuts at: 0 4710 7589 8175 12221 12795
Size: 4710 2879 586 4046 574
Fragments arranged by size:
4710 4046 2879 586 574
SacII CC_GC'GG
Cuts at: 0 7066 12795
Size: 7066 5729
SalI G'TCGA_C
Cuts at: 0 3507 12795
Size: 3507 9288
SanDI GG'GwC_CC
Cuts at: 0 86 12795
Size: 86 12709
SapI GCTCTTCn'nnn_
Cuts at: 0 2174 3556 5104 5404 8491 12795
Size: 2174 1382 1548 300 3087 4304
Fragments arranged by size:
4304 3087 2174 1548 1382 300
SbfI CC_TGCA'GG
Cuts at: 0 3416 5384 5491 12795
Size: 3416 1968 107 7304
Fragments arranged by size:
7304 3416 1968 107
ScaI AGT'ACT
Cuts at: 0 1060 5607 10414 10880 12068 12795
Size: 1060 4547 4807 466 1188 727
Fragments arranged by size:
4807 4547 1188 1060 727 466
SexAI A'CCwGG_T
Cuts at: 0 950 1985 2675 3923 12795
Size: 950 1035 690 1248 8872
Fragments arranged by size:
8872 1248 1035 950 690
SfcI C'TryA_G
Cuts at: 0 24 71 502 1819 1950 2013 2230 2866
Size: 24 47 431 1317 131 63 217 636
Cuts at: 2866 3412 5380 5487 5993 7900 8071 8956 9418
Size: 546 1968 107 506 1907 171 885 462
Cuts at: 9418 10214 10518 10527 11323 11388 11736 11933 12103
Size: 796 304 9 796 65 348 197 170
Cuts at: 12103 12298 12382 12795
Size: 195 84 413
Fragments arranged by size:
1968 1907 1317 885 796 796 636 546
506 462 431 413 348 304 217 197
195 171 170 131 107 84 65 63
47 24 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 4252 12795
Size: 4252 8543
SmaI CCC'GGG
Cuts at: 0 113 830 5054 7037 8837 8966 12795
Size: 113 717 4224 1983 1800 129 3829
Fragments arranged by size:
4224 3829 1983 1800 717 129 113
SmlI C'TyrA_G
Cuts at: 0 1590 1669 4717 5302 5392 5503 6214 6262
Size: 1590 79 3048 585 90 111 711 48
Cuts at: 6262 6397 6724 7477 7888 8977 9883 10420 10810
Size: 135 327 753 411 1089 906 537 390
Cuts at: 10810 11569 11614 11989 12136 12145 12247 12795
Size: 759 45 375 147 9 102 548
Fragments arranged by size:
3048 1590 1089 906 759 753 711 585
548 537 411 390 375 327 147 135
111 102 90 79 48 45 9
SpeI A'CTAG_T
Cuts at: 0 12069 12795
Size: 12069 726
SphI G_CATG'C
Cuts at: 0 1194 3318 4452 7350 12795
Size: 1194 2124 1134 2898 5445
Fragments arranged by size:
5445 2898 2124 1194 1134
StuI AGG'CCT
Cuts at: 0 508 977 2236 3577 4789 5485 12235 12304
Size: 508 469 1259 1341 1212 696 6750 69
Cuts at: 12304 12538 12795
Size: 234 257
Fragments arranged by size:
6750 1341 1259 1212 696 508 469 257
234 69
StyI C'CwwG_G
Cuts at: 0 2213 2243 2760 3242 3371 3806 4337 4496
Size: 2213 30 517 482 129 435 531 159
Cuts at: 4496 4790 4844 6566 6608 7025 7167 7331 7341
Size: 294 54 1722 42 417 142 164 10
Cuts at: 7341 7367 8081 8313 8591 8609 8918 9080 9089
Size: 26 714 232 278 18 309 162 9
Cuts at: 9089 9149 11397 11472 12230 12498 12563 12795
Size: 60 2248 75 758 268 65 232
Fragments arranged by size:
2248 2213 1722 758 714 531 517 482
435 417 309 294 278 268 232 232
164 162 159 142 129 75 65 60
54 42 30 26 18 10 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 219 1053 2095 2368 4307 5770 6146 6334
Size: 219 834 1042 273 1939 1463 376 188
Cuts at: 6334 6522 6939 7448 7750 7951 8821 9105 10372
Size: 188 417 509 302 201 870 284 1267
Cuts at: 10372 11989 12009 12624 12748 12795
Size: 1617 20 615 124 47
Fragments arranged by size:
1939 1617 1463 1267 1042 870 834 615
509 417 376 302 284 273 219 201
188 188 124 47 20
TatI w'GTAC_w
Cuts at: 0 896 1058 1604 1811 3525 4121 5605 7628
Size: 896 162 546 207 1714 596 1484 2023
Cuts at: 7628 8336 9422 9965 10334 10412 10878 11618 12066
Size: 708 1086 543 369 78 466 740 448
Cuts at: 12066 12072 12197 12572 12795
Size: 6 125 375 223
Fragments arranged by size:
2023 1714 1484 1086 896 740 708 596
546 543 466 448 375 369 223 207
162 125 78 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 439 471 1820 1852 3487 3519 6330 6362
Size: 439 32 1349 32 1635 32 2811 32
Cuts at: 6362 9200 9232 9307 9339 9899 9931 12747 12779
Size: 2838 32 75 32 560 32 2816 32
Cuts at: 12779 12795
Size: 16
Fragments arranged by size:
2838 2816 2811 1635 1349 560 439 75
32 32 32 32 32 32 32 32
16
Tth111I GACn'n_nGTC
Cuts at: 0 1918 2434 3339 7111 12795
Size: 1918 516 905 3772 5684
Fragments arranged by size:
5684 3772 1918 905 516
XbaI T'CTAG_A
Cuts at: 0 1290 12795
Size: 1290 11505
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2481 4776 7668 8418 8607 10980 12332 12795
Size: 2481 2295 2892 750 189 2373 1352 463
Fragments arranged by size:
2892 2481 2373 2295 1352 750 463 189
XhoI C'TCGA_G
Cuts at: 0 1590 12795
Size: 1590 11205
XmaI C'CCGG_G
Cuts at: 0 111 828 5052 7035 8835 8964 12795
Size: 111 717 4224 1983 1800 129 3831
Fragments arranged by size:
4224 3831 1983 1800 717 129 111
XmnI GAAnn'nnTTC
Cuts at: 0 2056 2842 3055 4546 12795
Size: 2056 786 213 1491 8249
Fragments arranged by size:
8249 2056 1491 786 213
ZraI GAC'GTC
Cuts at: 0 5639 11970 12795
Size: 5639 6331 825
Fragments arranged by size:
6331 5639 825
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BaeI
BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MmeI
MscI MslI MspA1I NaeI NcoI NgoMIV NotI NsiI
NspI PacI PasI PciI PflMI PfoI PmlI PpiI
PpuMI PspOMI PspXI PsrI PstI PvuI PvuII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AclI AflII AgeI AloI AscI AseI AsiSI AvrII
BbeI BmtI BsiWI BspEI BstBI CspCI DraI FseI
FspAI KasI MfeI MluI NarI NdeI NheI NruI
PmeI PshAI PsiI RsrII SfoI SgfI SgrAI SnaBI
SrfI SspI SwaI