Restriction map for
pF1KSDA1412
(Linear) (Six-base) MAPSORT of: KIAA1412.seq Check: 1092 from: 1 to: 4149
KIAA1412.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3585 4149
Size: 3585 564
AccI GT'mk_AC
Cuts at: 0 1436 4149
Size: 1436 2713
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 212 874 1565 4149
Size: 119 93 662 691 2584
Fragments arranged by size:
2584 691 662 119 93
AflIII A'CryG_T
Cuts at: 0 2304 2766 4149
Size: 2304 462 1383
Fragments arranged by size:
2304 1383 462
AhdI GACnn_n'nnGTC
Cuts at: 0 1713 4149
Size: 1713 2436
AleI CACnn'nnGTG
Cuts at: 0 1415 1658 4149
Size: 1415 243 2491
Fragments arranged by size:
2491 1415 243
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 594 1765 2979 4149
Size: 594 1171 1214 1170
Fragments arranged by size:
1214 1171 1170 594
AlwNI CAG_nnn'CTG
Cuts at: 0 1407 3428 3494 4149
Size: 1407 2021 66 655
Fragments arranged by size:
2021 1407 655 66
ApaI G_GGCC'C
Cuts at: 0 2630 4149
Size: 2630 1519
ApoI r'AATT_y
Cuts at: 0 142 741 833 1144 1254 1457 1572 2169
Size: 142 599 92 311 110 203 115 597
Cuts at: 2169 2391 2712 3352 4149
Size: 222 321 640 797
Fragments arranged by size:
797 640 599 597 321 311 222 203
142 115 110 92
AseI AT'TA_AT
Cuts at: 0 2024 3074 4149
Size: 2024 1050 1075
Fragments arranged by size:
2024 1075 1050
AvaI C'yCGr_G
Cuts at: 0 791 4149
Size: 791 3358
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2673 2706 4149
Size: 2673 33 1443
Fragments arranged by size:
2673 1443 33
BamHI G'GATC_C
Cuts at: 0 369 502 912 3750 4149
Size: 369 133 410 2838 399
Fragments arranged by size:
2838 410 399 369 133
BanI G'GyrC_C
Cuts at: 0 3153 4149
Size: 3153 996
BanII G_rGCy'C
Cuts at: 0 2630 4149
Size: 2630 1519
BbsI GAAGACnn'nnnn_
Cuts at: 0 170 4149
Size: 170 3979
BbvCI CC'TCA_GC
Cuts at: 0 1448 4149
Size: 1448 2701
BciVI GTATCCnnnnn_n'
Cuts at: 0 1678 2869 3053 4149
Size: 1678 1191 184 1096
Fragments arranged by size:
1678 1191 1096 184
Bme1580I G_kGCm'C
Cuts at: 0 705 2630 4149
Size: 705 1925 1519
Fragments arranged by size:
1925 1519 705
BmgBI CAC'GTC
Cuts at: 0 1668 4149
Size: 1668 2481
BmrI ACTGGGnnnn_n'
Cuts at: 0 1033 2096 4149
Size: 1033 1063 2053
Fragments arranged by size:
2053 1063 1033
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 436 4149
Size: 436 3713
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 709 1307 2570 2657 3139 3351 4149
Size: 308 401 598 1263 87 482 212 798
Fragments arranged by size:
1263 798 598 482 401 308 212 87
Bpu10I CC'TnA_GC
Cuts at: 0 1448 4149
Size: 1448 2701
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1719 4149
Size: 1719 2430
BsaI GGTCTCn'nnnn_
Cuts at: 0 1283 3671 4106 4149
Size: 1283 2388 435 43
Fragments arranged by size:
2388 1283 435 43
BsaAI yAC'GTr
Cuts at: 0 2305 4149
Size: 2305 1844
BsaBI GATnn'nnATC
Cuts at: 0 347 3041 4149
Size: 347 2694 1108
Fragments arranged by size:
2694 1108 347
BsaHI Gr'CG_yC
Cuts at: 0 437 3731 4149
Size: 437 3294 418
Fragments arranged by size:
3294 437 418
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2348 2378 4149
Size: 2348 30 1771
Fragments arranged by size:
2348 1771 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 104 107 1351 1858 2384 2387 3727 3890
Size: 104 3 1244 507 526 3 1340 163
Cuts at: 3890 4149
Size: 259
Fragments arranged by size:
1340 1244 526 507 259 163 104 3
3
BseYI C'CCAG_C
Cuts at: 0 3668 4149
Size: 3668 481
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1393 3746 3777 4149
Size: 1393 2353 31 372
Fragments arranged by size:
2353 1393 372 31
BsiHKAI G_wGCw'C
Cuts at: 0 2650 3007 3065 3871 4149
Size: 2650 357 58 806 278
Fragments arranged by size:
2650 806 357 278 58
BsmI GAATG_Cn'
Cuts at: 0 181 447 1369 2958 3312 4149
Size: 181 266 922 1589 354 837
Fragments arranged by size:
1589 922 837 354 266 181
BsmBI CGTCTCn'nnnn_
Cuts at: 0 361 4149
Size: 361 3788
Bsp1286I G_dGCh'C
Cuts at: 0 705 2630 2650 3007 3065 3871 4149
Size: 705 1925 20 357 58 806 278
Fragments arranged by size:
1925 806 705 357 278 58 20
BspHI T'CATG_A
Cuts at: 0 2705 4149
Size: 2705 1444
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3585 4149
Size: 3585 564
BsrDI GCAATG_nn'
Cuts at: 0 100 879 1089 2330 4149
Size: 100 779 210 1241 1819
Fragments arranged by size:
1819 1241 779 210 100
BsrFI r'CCGG_y
Cuts at: 0 3150 3552 4149
Size: 3150 402 597
Fragments arranged by size:
3150 597 402
BsrGI T'GTAC_A
Cuts at: 0 2991 4149
Size: 2991 1158
BssSI C'ACGA_G
Cuts at: 0 2749 3032 4149
Size: 2749 283 1117
Fragments arranged by size:
2749 1117 283
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1079 4149
Size: 1079 3070
BstXI CCAn_nnnn'nTGG
Cuts at: 0 550 837 3057 3145 3415 3969 4149
Size: 550 287 2220 88 270 554 180
Fragments arranged by size:
2220 554 550 287 270 180 88
BstYI r'GATC_y
Cuts at: 0 369 502 912 3458 3750 4149
Size: 369 133 410 2546 292 399
Fragments arranged by size:
2546 410 399 369 292 133
Bsu36I CC'TnA_GG
Cuts at: 0 747 4149
Size: 747 3402
BtgI C'CryG_G
Cuts at: 0 3157 4149
Size: 3157 992
BtsI GCAGTG_nn'
Cuts at: 0 1122 2694 2963 2973 4149
Size: 1122 1572 269 10 1176
Fragments arranged by size:
1572 1176 1122 269 10
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1685 1720 4149
Size: 1685 35 2429
Fragments arranged by size:
2429 1685 35
DraI TTT'AAA
Cuts at: 0 2340 3830 3990 4149
Size: 2340 1490 160 159
Fragments arranged by size:
2340 1490 160 159
DrdI GACnn_nn'nnGTC
Cuts at: 0 2200 4149
Size: 2200 1949
EaeI y'GGCC_r
Cuts at: 0 827 1161 3417 3500 3554 4149
Size: 827 334 2256 83 54 595
Fragments arranged by size:
2256 827 595 334 83 54
EarI CTCTTCn'nnn_
Cuts at: 0 1352 3815 4104 4149
Size: 1352 2463 289 45
Fragments arranged by size:
2463 1352 289 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2908 4149
Size: 2908 1241
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 212 308 709 874 1307 1565 2570
Size: 119 93 96 401 165 433 258 1005
Cuts at: 2570 2657 3139 3351 4149
Size: 87 482 212 798
Fragments arranged by size:
1005 798 482 433 401 258 212 165
119 96 93 87
EcoNI CCTnn'n_nnAGG
Cuts at: 0 541 4149
Size: 541 3608
EcoO109I rG'GnC_Cy
Cuts at: 0 734 764 795 2972 4032 4149
Size: 734 30 31 2177 1060 117
Fragments arranged by size:
2177 1060 734 117 31 30
EcoRI G'AATT_C
Cuts at: 0 741 1457 2712 4149
Size: 741 716 1255 1437
Fragments arranged by size:
1437 1255 741 716
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1094 2199 2666 3105 4149
Size: 1094 1105 467 439 1044
Fragments arranged by size:
1105 1094 1044 467 439
HaeII r_GCGC'y
Cuts at: 0 559 4149
Size: 559 3590
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 201 233 1699 1731 1989 2021 2093 2125
Size: 201 32 1466 32 258 32 72 32
Cuts at: 2125 2348 2366 2380 2398 2824 2856 2907 2939
Size: 223 18 14 18 426 32 51 32
Cuts at: 2939 3031 3063 3443 3475 4149
Size: 92 32 380 32 674
Fragments arranged by size:
1466 674 426 380 258 223 201 92
72 51 32 32 32 32 32 32
32 32 18 18 14
HincII GTy'rAC
Cuts at: 0 3183 4149
Size: 3183 966
HindIII A'AGCT_T
Cuts at: 0 131 1037 2442 4133 4149
Size: 131 906 1405 1691 16
Fragments arranged by size:
1691 1405 906 131 16
MfeI C'AATT_G
Cuts at: 0 2693 4149
Size: 2693 1456
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 901 1258 1434 3074 4149
Size: 66 835 357 176 1640 1075
Fragments arranged by size:
1640 1075 835 357 176 66
MscI TGG'CCA
Cuts at: 0 1163 3419 3502 4149
Size: 1163 2256 83 647
Fragments arranged by size:
2256 1163 647 83
MslI CAynn'nnrTG
Cuts at: 0 982 1115 1415 1658 1747 1957 2282 3143
Size: 982 133 300 243 89 210 325 861
Cuts at: 3143 3413 3944 4149
Size: 270 531 205
Fragments arranged by size:
982 861 531 325 300 270 243 210
205 133 89
MspA1I CmG'CkG
Cuts at: 0 2035 3267 4068 4149
Size: 2035 1232 801 81
Fragments arranged by size:
2035 1232 801 81
NaeI GCC'GGC
Cuts at: 0 3152 3554 4149
Size: 3152 402 595
Fragments arranged by size:
3152 595 402
NgoMIV G'CCGG_C
Cuts at: 0 3150 3552 4149
Size: 3150 402 597
Fragments arranged by size:
3150 597 402
NspI r_CATG'y
Cuts at: 0 1696 2770 4149
Size: 1696 1074 1379
Fragments arranged by size:
1696 1379 1074
PciI A'CATG_T
Cuts at: 0 2766 4149
Size: 2766 1383
PflMI CCAn_nnn'nTGG
Cuts at: 0 649 1023 1299 1860 3140 4149
Size: 649 374 276 561 1280 1009
Fragments arranged by size:
1280 1009 649 561 374 276
PfoI T'CCnGG_A
Cuts at: 0 372 542 908 989 4149
Size: 372 170 366 81 3160
Fragments arranged by size:
3160 372 366 170 81
PmlI CAC'GTG
Cuts at: 0 2305 4149
Size: 2305 1844
PpuMI rG'GwC_Cy
Cuts at: 0 734 764 2972 4149
Size: 734 30 2208 1177
Fragments arranged by size:
2208 1177 734 30
PsiI TTA'TAA
Cuts at: 0 1676 2150 4149
Size: 1676 474 1999
Fragments arranged by size:
1999 1676 474
PspOMI G'GGCC_C
Cuts at: 0 2626 4149
Size: 2626 1523
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 4040 4072 4149
Size: 4040 32 77
Fragments arranged by size:
4040 77 32
PstI C_TGCA'G
Cuts at: 0 2040 2968 4149
Size: 2040 928 1181
Fragments arranged by size:
2040 1181 928
PvuII CAG'CTG
Cuts at: 0 2035 4068 4149
Size: 2035 2033 81
Fragments arranged by size:
2035 2033 81
ScaI AGT'ACT
Cuts at: 0 3520 4149
Size: 3520 629
SfcI C'TryA_G
Cuts at: 0 452 1029 2036 2964 3522 4149
Size: 452 577 1007 928 558 627
Fragments arranged by size:
1007 928 627 577 558 452
SmaI CCC'GGG
Cuts at: 0 793 4149
Size: 793 3356
SmlI C'TyrA_G
Cuts at: 0 1734 4149
Size: 1734 2415
SpeI A'CTAG_T
Cuts at: 0 265 3607 3909 4149
Size: 265 3342 302 240
Fragments arranged by size:
3342 302 265 240
SspI AAT'ATT
Cuts at: 0 513 1473 4149
Size: 513 960 2676
Fragments arranged by size:
2676 960 513
StyI C'CwwG_G
Cuts at: 0 1017 1482 2233 3847 4035 4149
Size: 1017 465 751 1614 188 114
Fragments arranged by size:
1614 1017 751 465 188 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 83 1405 4149
Size: 83 1322 2744
Fragments arranged by size:
2744 1322 83
TatI w'GTAC_w
Cuts at: 0 2991 3518 4149
Size: 2991 527 631
Fragments arranged by size:
2991 631 527
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1335 1367 4149
Size: 1335 32 2782
Fragments arranged by size:
2782 1335 32
Tth111I GACn'n_nGTC
Cuts at: 0 3572 4149
Size: 3572 577
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 49 2857 4149
Size: 49 2808 1292
Fragments arranged by size:
2808 1292 49
XmaI C'CCGG_G
Cuts at: 0 791 4149
Size: 791 3358
XmnI GAAnn'nnTTC
Cuts at: 0 1074 4149
Size: 1074 3075
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AleI AlfI AlwNI
ApaI ApoI AseI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BciVI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrDI BsrFI BsrGI BssSI BstAPI BstXI BstYI Bsu36I
BtgI BtsI CspCI DraI DrdI EaeI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII
HindIII MfeI MmeI MscI MslI MspA1I NaeI NgoMIV
NspI PciI PflMI PfoI PmlI PpuMI PsiI PspOMI
PsrI PstI PvuII ScaI SfcI SmaI SmlI SpeI
SspI StyI TaqII TatI TstI Tth111I XcmI XmaI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AloI ApaLI
AscI AsiSI AvrII BbeI BcgI BclI BfrBI BglI
BglII BlpI BmtI BsaWI BsiEI BsiWI BspEI BsrBI
BssHII BstBI BstEII BstZ17I BtgZI ClaI DraIII EagI
EcoICRI EcoRV FseI FspI FspAI HpaI KasI KpnI
MluI NarI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PmeI PpiI PshAI PspXI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SnaBI SphI SrfI StuI SwaI
XbaI XhoI ZraI