Restriction map for
pF1KA1419
(Linear) (Six-base) MAPSORT of: KIAA1419.seq Check: 6788 from: 1 to: 7296
KIAA1419.orf
With 182 enzymes: SgfI *
May 27, 2009 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 171 672 990 1237 2883 7296
Size: 171 501 318 247 1646 4413
Fragments arranged by size:
4413 1646 501 318 247 171
AatII G_ACGT'C
Cuts at: 0 2528 6842 7296
Size: 2528 4314 454
Fragments arranged by size:
4314 2528 454
AccI GT'mk_AC
Cuts at: 0 4483 6038 7296
Size: 4483 1555 1258
Fragments arranged by size:
4483 1555 1258
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 537 1184 2069 3146 3202 3547 4540
Size: 136 401 647 885 1077 56 345 993
Cuts at: 4540 5719 6633 7178 7296
Size: 1179 914 545 118
Fragments arranged by size:
1179 1077 993 914 885 647 545 401
345 136 118 56
AfeI AGC'GCT
Cuts at: 0 3265 6256 7296
Size: 3265 2991 1040
Fragments arranged by size:
3265 2991 1040
AflIII A'CryG_T
Cuts at: 0 5825 7296
Size: 5825 1471
AhdI GACnn_n'nnGTC
Cuts at: 0 4020 6414 7296
Size: 4020 2394 882
Fragments arranged by size:
4020 2394 882
AleI CACnn'nnGTG
Cuts at: 0 1351 7296
Size: 1351 5945
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1070 2927 3160 4532 6445 7156 7296
Size: 1070 1857 233 1372 1913 711 140
Fragments arranged by size:
1913 1857 1372 1070 711 233 140
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 238 270 7296
Size: 238 32 7026
Fragments arranged by size:
7026 238 32
AlwNI CAG_nnn'CTG
Cuts at: 0 186 195 498 3196 5043 5723 6181 6441
Size: 186 9 303 2698 1847 680 458 260
Cuts at: 6441 7296
Size: 855
Fragments arranged by size:
2698 1847 855 680 458 303 260 186
9
ApaI G_GGCC'C
Cuts at: 0 100 648 1360 7296
Size: 100 548 712 5936
Fragments arranged by size:
5936 712 548 100
ApaLI G'TGCA_C
Cuts at: 0 52 7296
Size: 52 7244
ApoI r'AATT_y
Cuts at: 0 462 2205 2940 3960 5487 6063 7296
Size: 462 1743 735 1020 1527 576 1233
Fragments arranged by size:
1743 1527 1233 1020 735 576 462
AscI GG'CGCG_CC
Cuts at: 0 6981 7296
Size: 6981 315
AseI AT'TA_AT
Cuts at: 0 5141 7296
Size: 5141 2155
AvaI C'yCGr_G
Cuts at: 0 89 1265 1588 1717 2417 2498 5763 6891
Size: 89 1176 323 129 700 81 3265 1128
Cuts at: 6891 7115 7296
Size: 224 181
Fragments arranged by size:
3265 1176 1128 700 323 224 181 129
89 81
AvrII C'CTAG_G
Cuts at: 0 3711 7296
Size: 3711 3585
BamHI G'GATC_C
Cuts at: 0 4837 6996 7296
Size: 4837 2159 300
Fragments arranged by size:
4837 2159 300
BanI G'GyrC_C
Cuts at: 0 667 684 4153 7296
Size: 667 17 3469 3143
Fragments arranged by size:
3469 3143 667 17
BanII G_rGCy'C
Cuts at: 0 100 221 648 1240 1360 1667 1786 2099
Size: 100 121 427 592 120 307 119 313
Cuts at: 2099 3516 5168 5736 6194 6532 7296
Size: 1417 1652 568 458 338 764
Fragments arranged by size:
1652 1417 764 592 568 458 427 338
313 307 121 120 119 100
BbsI GAAGACnn'nnnn_
Cuts at: 0 407 1571 3190 4357 7296
Size: 407 1164 1619 1167 2939
Fragments arranged by size:
2939 1619 1167 1164 407
BbvCI CC'TCA_GC
Cuts at: 0 674 1087 1973 2821 4252 4690 5482 7296
Size: 674 413 886 848 1431 438 792 1814
Fragments arranged by size:
1814 1431 886 848 792 674 438 413
BciVI GTATCCnnnnn_n'
Cuts at: 0 639 804 1122 2420 2638 7296
Size: 639 165 318 1298 218 4658
Fragments arranged by size:
4658 1298 639 318 218 165
BclI T'GATC_A
Cuts at: 0 3401 7296
Size: 3401 3895
BfrBI ATG'CAT
Cuts at: 0 5519 7296
Size: 5519 1777
BglI GCCn_nnn'nGGC
Cuts at: 0 665 1137 3394 4126 4802 6308 7296
Size: 665 472 2257 732 676 1506 988
Fragments arranged by size:
2257 1506 988 732 676 665 472
BglII A'GATC_T
Cuts at: 0 266 6542 7296
Size: 266 6276 754
Fragments arranged by size:
6276 754 266
BlpI GC'TnA_GC
Cuts at: 0 1780 4116 5057 7296
Size: 1780 2336 941 2239
Fragments arranged by size:
2336 2239 1780 941
Bme1580I G_kGCm'C
Cuts at: 0 56 83 100 648 1360 1422 1589 1737
Size: 56 27 17 548 712 62 167 148
Cuts at: 1737 3683 3935 4156 7296
Size: 1946 252 221 3140
Fragments arranged by size:
3140 1946 712 548 252 221 167 148
62 56 27 17
BmgBI CAC'GTC
Cuts at: 0 3068 7296
Size: 3068 4228
BmrI ACTGGGnnnn_n'
Cuts at: 0 288 648 1565 2153 2362 3454 3581 5187
Size: 288 360 917 588 209 1092 127 1606
Cuts at: 5187 5331 6037 6323 7142 7296
Size: 144 706 286 819 154
Fragments arranged by size:
1606 1092 917 819 706 588 360 288
286 209 154 144 127
BmtI G_CTAG'C
Cuts at: 0 4102 4394 7296
Size: 4102 292 2902
Fragments arranged by size:
4102 2902 292
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2091 2940 5313 5507 6979 7296
Size: 2091 849 2373 194 1472 317
Fragments arranged by size:
2373 2091 1472 849 317 194
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 1309 1345 1812 2367 3838 3881 4089
Size: 316 993 36 467 555 1471 43 208
Cuts at: 4089 4578 5020 5226 5253 6838 6949 7197 7296
Size: 489 442 206 27 1585 111 248 99
Fragments arranged by size:
1585 1471 993 555 489 467 442 316
248 208 206 111 99 43 36 27
Bpu10I CC'TnA_GC
Cuts at: 0 476 674 1087 1785 1973 2821 3128 3544
Size: 476 198 413 698 188 848 307 416
Cuts at: 3544 4039 4252 4690 5162 5482 7296
Size: 495 213 438 472 320 1814
Fragments arranged by size:
1814 848 698 495 476 472 438 416
413 320 307 213 198 188
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 1372 1531 5696 7296
Size: 514 858 159 4165 1600
Fragments arranged by size:
4165 1600 858 514 159
BsaI GGTCTCn'nnnn_
Cuts at: 0 2039 2716 5530 7296
Size: 2039 677 2814 1766
Fragments arranged by size:
2814 2039 1766 677
BsaAI yAC'GTr
Cuts at: 0 78 6525 7296
Size: 78 6447 771
Fragments arranged by size:
6447 771 78
BsaHI Gr'CG_yC
Cuts at: 0 353 1849 2387 2525 2576 4034 6839 7296
Size: 353 1496 538 138 51 1458 2805 457
Fragments arranged by size:
2805 1496 1458 538 457 353 138 51
BsaWI w'CCGG_w
Cuts at: 0 2621 3569 4612 4751 6126 7296
Size: 2621 948 1043 139 1375 1170
Fragments arranged by size:
2621 1375 1170 1043 948 139
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1568 1598 1755 1785 2767 2797 2830 2860
Size: 1568 30 157 30 982 30 33 30
Cuts at: 2860 3279 3309 4000 4030 4041 4071 4690 4720
Size: 419 30 691 30 11 30 619 30
Cuts at: 4720 5224 5254 7090 7120 7222 7252 7296
Size: 504 30 1836 30 102 30 44
Fragments arranged by size:
1836 1568 982 691 619 504 419 157
102 44 33 30 30 30 30 30
30 30 30 30 30 30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 597 942 1023 1333 1567 2838 2871 4161
Size: 597 345 81 310 234 1271 33 1290
Cuts at: 4161 4293 5217 5422 6726 7266 7296
Size: 132 924 205 1304 540 30
Fragments arranged by size:
1304 1290 1271 924 597 540 345 310
234 205 132 81 33 30
BseYI C'CCAG_C
Cuts at: 0 101 1300 1737 1796 1808 1979 2098 2768
Size: 101 1199 437 59 12 171 119 670
Cuts at: 2768 3319 3613 3821 4111 4270 4496 4634 4967
Size: 551 294 208 290 159 226 138 333
Cuts at: 4967 5786 6304 7296
Size: 819 518 992
Fragments arranged by size:
1199 992 819 670 551 518 437 333
294 290 226 208 171 159 138 119
101 59 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 1076 2923 3452 7296
Size: 58 1018 1847 529 3844
Fragments arranged by size:
3844 1847 1018 529 58
BsiHKAI G_wGCw'C
Cuts at: 0 56 1786 5168 5993 6281 6532 7296
Size: 56 1730 3382 825 288 251 764
Fragments arranged by size:
3382 1730 825 764 288 251 56
BsmI GAATG_Cn'
Cuts at: 0 5521 5854 7296
Size: 5521 333 1442
Fragments arranged by size:
5521 1442 333
BsmBI CGTCTCn'nnnn_
Cuts at: 0 322 2378 2532 2750 5244 6267 7296
Size: 322 2056 154 218 2494 1023 1029
Fragments arranged by size:
2494 2056 1029 1023 322 218 154
Bsp1286I G_dGCh'C
Cuts at: 0 56 83 100 221 648 1240 1360 1422
Size: 56 27 17 121 427 592 120 62
Cuts at: 1422 1589 1667 1737 1786 2099 3516 3683 3935
Size: 167 78 70 49 313 1417 167 252
Cuts at: 3935 4156 5168 5736 5993 6194 6281 6532 7296
Size: 221 1012 568 257 201 87 251 764
Fragments arranged by size:
1417 1012 764 592 568 427 313 257
252 251 221 201 167 167 121 120
87 78 70 62 56 49 27 17
BspEI T'CCGG_A
Cuts at: 0 2621 4612 4751 7296
Size: 2621 1991 139 2545
Fragments arranged by size:
2621 2545 1991 139
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 171 672 990 1237 2423 2883 3422 6638
Size: 171 501 318 247 1186 460 539 3216
Cuts at: 6638 7296
Size: 658
Fragments arranged by size:
3216 1186 658 539 501 460 318 247
171
BsrBI CCG'CTC
Cuts at: 0 236 998 1284 1761 7296
Size: 236 762 286 477 5535
Fragments arranged by size:
5535 762 477 286 236
BsrDI GCAATG_nn'
Cuts at: 0 1394 1458 2409 7296
Size: 1394 64 951 4887
Fragments arranged by size:
4887 1394 951 64
BsrFI r'CCGG_y
Cuts at: 0 3 1176 2690 3388 6335 7296
Size: 3 1173 1514 698 2947 961
Fragments arranged by size:
2947 1514 1173 961 698 3
BsrGI T'GTAC_A
Cuts at: 0 2027 7296
Size: 2027 5269
BssHII G'CGCG_C
Cuts at: 0 6981 7296
Size: 6981 315
BssSI C'ACGA_G
Cuts at: 0 922 7051 7296
Size: 922 6129 245
Fragments arranged by size:
6129 922 245
BstAPI GCAn_nnn'nTGC
Cuts at: 0 186 195 684 5723 7296
Size: 186 9 489 5039 1573
Fragments arranged by size:
5039 1573 489 186 9
BstBI TT'CG_AA
Cuts at: 0 1307 7296
Size: 1307 5989
BstEII G'GTnAC_C
Cuts at: 0 4006 7162 7296
Size: 4006 3156 134
Fragments arranged by size:
4006 3156 134
BstXI CCAn_nnnn'nTGG
Cuts at: 0 4770 6882 6973 7296
Size: 4770 2112 91 323
Fragments arranged by size:
4770 2112 323 91
BstYI r'GATC_y
Cuts at: 0 164 266 327 427 2151 4837 6542 6996
Size: 164 102 61 100 1724 2686 1705 454
Cuts at: 6996 7296
Size: 300
Fragments arranged by size:
2686 1724 1705 454 300 164 102 100
61
BstZ17I GTA'TAC
Cuts at: 0 4484 7296
Size: 4484 2812
Bsu36I CC'TnA_GG
Cuts at: 0 1142 2156 6709 7082 7296
Size: 1142 1014 4553 373 214
Fragments arranged by size:
4553 1142 1014 373 214
BtgI C'CryG_G
Cuts at: 0 1106 1352 1667 2009 2288 5774 7027 7296
Size: 1106 246 315 342 279 3486 1253 269
Fragments arranged by size:
3486 1253 1106 342 315 279 269 246
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2172 2609 3921 4281 7296
Size: 2172 437 1312 360 3015
Fragments arranged by size:
3015 2172 1312 437 360
BtsI GCAGTG_nn'
Cuts at: 0 2003 3297 4035 4050 4263 5186 5476 5724
Size: 2003 1294 738 15 213 923 290 248
Cuts at: 5724 7296
Size: 1572
Fragments arranged by size:
2003 1572 1294 923 738 290 248 213
15
ClaI AT'CG_AT
Cuts at: 0 6878 7296
Size: 6878 418
DraIII CAC_nnn'GTG
Cuts at: 0 4992 6525 7296
Size: 4992 1533 771
Fragments arranged by size:
4992 1533 771
DrdI GACnn_nn'nnGTC
Cuts at: 0 776 2281 4359 7296
Size: 776 1505 2078 2937
Fragments arranged by size:
2937 2078 1505 776
EaeI y'GGCC_r
Cuts at: 0 5 306 638 1037 2301 3395 6329 6699
Size: 5 301 332 399 1264 1094 2934 370
Cuts at: 6699 7296
Size: 597
Fragments arranged by size:
2934 1264 1094 597 399 370 332 301
5
EarI CTCTTCn'nnn_
Cuts at: 0 226 503 553 1304 1429 1702 1842 4383
Size: 226 277 50 751 125 273 140 2541
Cuts at: 4383 4774 6621 6865 7296
Size: 391 1847 244 431
Fragments arranged by size:
2541 1847 751 431 391 277 273 244
226 140 125 50
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2460 3042 3372 7296
Size: 2460 582 330 3924
Fragments arranged by size:
3924 2460 582 330
EcoICRI GAG'CTC
Cuts at: 0 1784 5166 6530 7296
Size: 1784 3382 1364 766
Fragments arranged by size:
3382 1784 1364 766
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 316 537 1184 1309 1345 1812 2069
Size: 136 180 221 647 125 36 467 257
Cuts at: 2069 2367 3146 3202 3547 3838 3881 4089 4540
Size: 298 779 56 345 291 43 208 451
Cuts at: 4540 4578 5020 5226 5253 5719 6633 6838 6949
Size: 38 442 206 27 466 914 205 111
Cuts at: 6949 7178 7197 7296
Size: 229 19 99
Fragments arranged by size:
914 779 647 467 466 451 442 345
298 291 257 229 221 208 206 205
180 136 125 111 99 56 43 38
36 27 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1549 4469 5763 7296
Size: 1549 2920 1294 1533
Fragments arranged by size:
2920 1549 1533 1294
EcoO109I rG'GnC_Cy
Cuts at: 0 97 644 645 778 1146 1262 1357 1506
Size: 97 547 1 133 368 116 95 149
Cuts at: 1506 4266 7296
Size: 2760 3030
Fragments arranged by size:
3030 2760 547 368 149 133 116 97
95 1
EcoRI G'AATT_C
Cuts at: 0 2940 5487 6063 7296
Size: 2940 2547 576 1233
Fragments arranged by size:
2940 2547 1233 576
EcoRV GAT'ATC
Cuts at: 0 5592 7296
Size: 5592 1704
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3253 7296
Size: 3253 4043
FspI TGC'GCA
Cuts at: 0 6751 7296
Size: 6751 545
HaeII r_GCGC'y
Cuts at: 0 62 1744 3267 6258 7296
Size: 62 1682 1523 2991 1038
Fragments arranged by size:
2991 1682 1523 1038 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 138 170 2377 2409 2654 2686 2767 2799
Size: 138 32 2207 32 245 32 81 32
Cuts at: 2799 2968 3000 3015 3047 3868 3900 4006 4038
Size: 169 32 15 32 821 32 106 32
Cuts at: 4038 4041 4073 4829 4861 5224 5256 5924 5956
Size: 3 32 756 32 363 32 668 32
Cuts at: 5956 6400 6432 6953 6985 7090 7122 7222 7254
Size: 444 32 521 32 105 32 100 32
Cuts at: 7254 7296
Size: 42
Fragments arranged by size:
2207 821 756 668 521 444 363 245
169 138 106 105 100 81 42 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 15
3
HincII GTy'rAC
Cuts at: 0 833 3738 5813 6039 6377 7296
Size: 833 2905 2075 226 338 919
Fragments arranged by size:
2905 2075 919 833 338 226
HindIII A'AGCT_T
Cuts at: 0 5354 7296
Size: 5354 1942
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3080 3472 3620 3671 4160 4700 5522 6135
Size: 3080 392 148 51 489 540 822 613
Cuts at: 6135 6265 6470 7265 7296
Size: 130 205 795 31
Fragments arranged by size:
3080 822 795 613 540 489 392 205
148 130 51 31
MscI TGG'CCA
Cuts at: 0 3397 6331 7296
Size: 3397 2934 965
Fragments arranged by size:
3397 2934 965
MslI CAynn'nnrTG
Cuts at: 0 1351 2293 2569 3001 3142 4663 6175 6880
Size: 1351 942 276 432 141 1521 1512 705
Cuts at: 6880 7026 7183 7259 7296
Size: 146 157 76 37
Fragments arranged by size:
1521 1512 1351 942 705 432 276 157
146 141 76 37
MspA1I CmG'CkG
Cuts at: 0 208 225 799 1479 1582 1812 2490 2768
Size: 208 17 574 680 103 230 678 278
Cuts at: 2768 2795 2993 3413 3946 4229 4567 4971 5056
Size: 27 198 420 533 283 338 404 85
Cuts at: 5056 5125 5625 6308 7170 7296
Size: 69 500 683 862 126
Fragments arranged by size:
862 683 680 678 574 533 500 420
404 338 283 278 230 208 198 126
103 85 69 27 17
NaeI GCC'GGC
Cuts at: 0 5 1178 3390 7296
Size: 5 1173 2212 3906
Fragments arranged by size:
3906 2212 1173 5
NcoI C'CATG_G
Cuts at: 0 1667 5774 7027 7296
Size: 1667 4107 1253 269
Fragments arranged by size:
4107 1667 1253 269
NgoMIV G'CCGG_C
Cuts at: 0 3 1176 3388 7296
Size: 3 1173 2212 3908
Fragments arranged by size:
3908 2212 1173 3
NheI G'CTAG_C
Cuts at: 0 4098 4390 7296
Size: 4098 292 2906
Fragments arranged by size:
4098 2906 292
NsiI A_TGCA'T
Cuts at: 0 5521 7296
Size: 5521 1775
NspI r_CATG'y
Cuts at: 0 1344 1716 5829 5881 6492 7182 7296
Size: 1344 372 4113 52 611 690 114
Fragments arranged by size:
4113 1344 690 611 372 114 52
PasI CC'CwG_GG
Cuts at: 0 2745 6989 7296
Size: 2745 4244 307
Fragments arranged by size:
4244 2745 307
PciI A'CATG_T
Cuts at: 0 5825 7296
Size: 5825 1471
PflMI CCAn_nnn'nTGG
Cuts at: 0 381 1070 3196 5625 6794 7296
Size: 381 689 2126 2429 1169 502
Fragments arranged by size:
2429 2126 1169 689 502 381
PfoI T'CCnGG_A
Cuts at: 0 2344 3550 4811 5636 6506 6815 6941 7296
Size: 2344 1206 1261 825 870 309 126 355
Fragments arranged by size:
2344 1261 1206 870 825 355 309 126
PmlI CAC'GTG
Cuts at: 0 78 6525 7296
Size: 78 6447 771
Fragments arranged by size:
6447 771 78
PpuMI rG'GwC_Cy
Cuts at: 0 778 1262 1506 4266 7296
Size: 778 484 244 2760 3030
Fragments arranged by size:
3030 2760 778 484 244
PspOMI G'GGCC_C
Cuts at: 0 96 644 1356 7296
Size: 96 548 712 5940
Fragments arranged by size:
5940 712 548 96
PspXI vC'TCGA_Gb
Cuts at: 0 2417 7296
Size: 2417 4879
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2229 2261 7296
Size: 2229 32 5035
Fragments arranged by size:
5035 2229 32
PstI C_TGCA'G
Cuts at: 0 191 200 507 804 4408 4548 5202 5728
Size: 191 9 307 297 3604 140 654 526
Cuts at: 5728 6254 6635 7073 7196 7296
Size: 526 381 438 123 100
Fragments arranged by size:
3604 654 526 526 438 381 307 297
191 140 123 100 9
PvuII CAG'CTG
Cuts at: 0 1582 1812 2490 2768 3946 4229 4971 5056
Size: 1582 230 678 278 1178 283 742 85
Cuts at: 5056 5125 5625 7296
Size: 69 500 1671
Fragments arranged by size:
1671 1582 1178 742 678 500 283 278
230 85 69
RsrII CG'GwC_CG
Cuts at: 0 2624 7296
Size: 2624 4672
SacI G_AGCT'C
Cuts at: 0 1786 5168 6532 7296
Size: 1786 3382 1364 764
Fragments arranged by size:
3382 1786 1364 764
SalI G'TCGA_C
Cuts at: 0 6037 7296
Size: 6037 1259
SanDI GG'GwC_CC
Cuts at: 0 1262 4266 7296
Size: 1262 3004 3030
Fragments arranged by size:
3030 3004 1262
SapI GCTCTTCn'nnn_
Cuts at: 0 226 1702 4774 6865 7296
Size: 226 1476 3072 2091 431
Fragments arranged by size:
3072 2091 1476 431 226
ScaI AGT'ACT
Cuts at: 0 4413 7296
Size: 4413 2883
SexAI A'CCwGG_T
Cuts at: 0 3726 7296
Size: 3726 3570
SfcI C'TryA_G
Cuts at: 0 187 196 503 800 4065 4404 4544 5198
Size: 187 9 307 297 3265 339 140 654
Cuts at: 5198 5724 6084 6250 6631 7069 7192 7296
Size: 526 360 166 381 438 123 104
Fragments arranged by size:
3265 654 526 438 381 360 339 307
297 187 166 140 123 104 9
SmaI CCC'GGG
Cuts at: 0 91 1267 1719 2500 7296
Size: 91 1176 452 781 4796
Fragments arranged by size:
4796 1176 781 452 91
SmlI C'TyrA_G
Cuts at: 0 529 1387 1546 2417 5711 6891 7296
Size: 529 858 159 871 3294 1180 405
Fragments arranged by size:
3294 1180 871 858 529 405 159
SphI G_CATG'C
Cuts at: 0 1344 1716 7296
Size: 1344 372 5580
Fragments arranged by size:
5580 1344 372
SrfI GCCC'GGGC
Cuts at: 0 91 7296
Size: 91 7205
StuI AGG'CCT
Cuts at: 0 545 3671 4553 7296
Size: 545 3126 882 2743
Fragments arranged by size:
3126 2743 882 545
StyI C'CwwG_G
Cuts at: 0 989 1069 1667 1748 3148 3711 4307 4850
Size: 989 80 598 81 1400 563 596 543
Cuts at: 4850 5774 6696 6788 7027 7296
Size: 924 922 92 239 269
Fragments arranged by size:
1400 989 924 922 598 596 563 543
269 239 92 81 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1113 1948 2641 3470 4675 4981 6645 6999
Size: 1113 835 693 829 1205 306 1664 354
Cuts at: 6999 7296
Size: 297
Fragments arranged by size:
1664 1205 1113 835 829 693 354 306
297
TatI w'GTAC_w
Cuts at: 0 2027 2465 4411 7296
Size: 2027 438 1946 2885
Fragments arranged by size:
2885 2027 1946 438
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 116 148 1568 1600 1755 1787 1816 1848
Size: 116 32 1420 32 155 32 29 32
Cuts at: 1848 3279 3311 4267 4299 4690 4722 5443 5475
Size: 1431 32 956 32 391 32 721 32
Cuts at: 5475 5646 5678 5899 5931 7296
Size: 171 32 221 32 1365
Fragments arranged by size:
1431 1420 1365 956 721 391 221 171
155 116 32 32 32 32 32 32
32 32 32 32 29
Tth111I GACn'n_nGTC
Cuts at: 0 5236 7296
Size: 5236 2060
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 661 1912 3017 6402 7011 7296
Size: 661 1251 1105 3385 609 285
Fragments arranged by size:
3385 1251 1105 661 609 285
XhoI C'TCGA_G
Cuts at: 0 2417 6891 7296
Size: 2417 4474 405
Fragments arranged by size:
4474 2417 405
XmaI C'CCGG_G
Cuts at: 0 89 1265 1717 2498 7296
Size: 89 1176 452 781 4798
Fragments arranged by size:
4798 1176 781 452 89
XmnI GAAnn'nnTTC
Cuts at: 0 1219 3237 4829 6691 7296
Size: 1219 2018 1592 1862 605
Fragments arranged by size:
2018 1862 1592 1219 605
ZraI GAC'GTC
Cuts at: 0 2526 6840 7296
Size: 2526 4314 456
Fragments arranged by size:
4314 2526 456
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AvaI AvrII BamHI BanI BanII BbsI BbvCI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI DraIII DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV NheI NsiI NspI PasI
PciI PflMI PfoI PmlI PpuMI PspOMI PspXI PsrI
PstI PvuII RsrII SacI SalI SanDI SapI ScaI
SexAI SfcI SmaI SmlI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AsiSI BaeI BbeI BcgI
BsaBI BsiEI BsiWI BspHI CspCI DraI EagI FseI
FspAI HpaI KasI KpnI MfeI MluI NarI NdeI
NotI NruI PacI PmeI PpiI PshAI PsiI PvuI
SacII SbfI SfiI SfoI SgfI SgrAI SnaBI SpeI
SspI SwaI XbaI