Restriction map for
pF1KA1426
(Linear) (Six-base) MAPSORT of: KIAA1426.seq Check: 9385 from: 1 to: 3195
KIAA1426.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1302 2042 3195
Size: 1302 740 1153
Fragments arranged by size:
1302 1153 740
AatII G_ACGT'C
Cuts at: 0 2603 3195
Size: 2603 592
AccI GT'mk_AC
Cuts at: 0 1460 3195
Size: 1460 1735
Acc65I G'GTAC_C
Cuts at: 0 2843 3195
Size: 2843 352
AclI AA'CG_TT
Cuts at: 0 2099 3195
Size: 2099 1096
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 298 1609 2327 3195
Size: 118 180 1311 718 868
Fragments arranged by size:
1311 868 718 180 118
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 419 2974 3195
Size: 419 2555 221
Fragments arranged by size:
2555 419 221
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1732 1764 3195
Size: 1732 32 1431
Fragments arranged by size:
1732 1431 32
AlwNI CAG_nnn'CTG
Cuts at: 0 117 729 1968 3195
Size: 117 612 1239 1227
Fragments arranged by size:
1239 1227 612 117
ApaI G_GGCC'C
Cuts at: 0 276 390 1517 3195
Size: 276 114 1127 1678
Fragments arranged by size:
1678 1127 276 114
ApaLI G'TGCA_C
Cuts at: 0 634 658 3195
Size: 634 24 2537
Fragments arranged by size:
2537 634 24
ApoI r'AATT_y
Cuts at: 0 466 3195
Size: 466 2729
AvaI C'yCGr_G
Cuts at: 0 89 517 758 871 894 992 1108 1236
Size: 89 428 241 113 23 98 116 128
Cuts at: 1236 1249 2367 3195
Size: 13 1118 828
Fragments arranged by size:
1118 828 428 241 128 116 113 98
89 23 13
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2835 2868 3195
Size: 2835 33 327
Fragments arranged by size:
2835 327 33
BanI G'GyrC_C
Cuts at: 0 932 1830 2030 2359 2843 3195
Size: 932 898 200 329 484 352
Fragments arranged by size:
932 898 484 352 329 200
BanII G_rGCy'C
Cuts at: 0 276 390 844 1052 1256 1307 1324 1517
Size: 276 114 454 208 204 51 17 193
Cuts at: 1517 2428 2494 2575 3195
Size: 911 66 81 620
Fragments arranged by size:
911 620 454 276 208 204 193 114
81 66 51 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 517 967 2159 2333 3195
Size: 517 450 1192 174 862
Fragments arranged by size:
1192 862 517 450 174
BbvCI CC'TCA_GC
Cuts at: 0 791 1576 3195
Size: 791 785 1619
Fragments arranged by size:
1619 791 785
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1878 1912 3195
Size: 1878 34 1283
Fragments arranged by size:
1878 1283 34
BclI T'GATC_A
Cuts at: 0 75 3195
Size: 75 3120
BlpI GC'TnA_GC
Cuts at: 0 1397 2872 3125 3195
Size: 1397 1475 253 70
Fragments arranged by size:
1475 1397 253 70
Bme1580I G_kGCm'C
Cuts at: 0 276 390 638 662 754 1517 2207 3195
Size: 276 114 248 24 92 763 690 988
Fragments arranged by size:
988 763 690 276 248 114 92 24
BmrI ACTGGGnnnn_n'
Cuts at: 0 953 1928 2147 2832 2843 3195
Size: 953 975 219 685 11 352
Fragments arranged by size:
975 953 685 352 219 11
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1829 2425 3195
Size: 1829 596 770
Fragments arranged by size:
1829 770 596
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 438 1060 1291 1516 1827 2518 2559 2726
Size: 438 622 231 225 311 691 41 167
Cuts at: 2726 3195
Size: 469
Fragments arranged by size:
691 622 469 438 311 231 225 167
41
Bpu10I CC'TnA_GC
Cuts at: 0 791 889 1576 2459 3195
Size: 791 98 687 883 736
Fragments arranged by size:
883 791 736 687 98
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 324 976 3195
Size: 324 652 2219
Fragments arranged by size:
2219 652 324
BsaI GGTCTCn'nnnn_
Cuts at: 0 456 3195
Size: 456 2739
BsaAI yAC'GTr
Cuts at: 0 708 3195
Size: 708 2487
BsaBI GATnn'nnATC
Cuts at: 0 2701 3195
Size: 2701 494
BsaHI Gr'CG_yC
Cuts at: 0 701 830 1687 2600 3195
Size: 701 129 857 913 595
Fragments arranged by size:
913 857 701 595 129
BsaWI w'CCGG_w
Cuts at: 0 844 2710 3195
Size: 844 1866 485
Fragments arranged by size:
1866 844 485
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 529 559 1663 1693 1732 1762 3195
Size: 529 30 1104 30 39 30 1433
Fragments arranged by size:
1433 1104 529 39 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 535 914 1315 1426 1564 1687 1785 2856
Size: 535 379 401 111 138 123 98 1071
Cuts at: 2856 3195
Size: 339
Fragments arranged by size:
1071 535 401 379 339 138 123 111
98
BseYI C'CCAG_C
Cuts at: 0 717 1480 1509 1777 2801 2968 3044 3195
Size: 717 763 29 268 1024 167 76 151
Fragments arranged by size:
1024 763 717 268 167 151 76 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 559 636 1769 3195
Size: 415 144 77 1133 1426
Fragments arranged by size:
1426 1133 415 144 77
BsiEI CG_ry'CG
Cuts at: 0 678 1521 2197 2201 3195
Size: 678 843 676 4 994
Fragments arranged by size:
994 843 678 676 4
BsiHKAI G_wGCw'C
Cuts at: 0 638 662 844 1052 1307 2575 3195
Size: 638 24 182 208 255 1268 620
Fragments arranged by size:
1268 638 620 255 208 182 24
BsmI GAATG_Cn'
Cuts at: 0 186 3195
Size: 186 3009
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1346 2607 3195
Size: 1346 1261 588
Fragments arranged by size:
1346 1261 588
Bsp1286I G_dGCh'C
Cuts at: 0 276 390 638 662 754 844 1052 1256
Size: 276 114 248 24 92 90 208 204
Cuts at: 1256 1307 1324 1517 2207 2428 2494 2575 3195
Size: 51 17 193 690 221 66 81 620
Fragments arranged by size:
690 620 276 248 221 208 204 193
114 92 90 81 66 51 24 17
BspEI T'CCGG_A
Cuts at: 0 844 3195
Size: 844 2351
BspHI T'CATG_A
Cuts at: 0 1466 3195
Size: 1466 1729
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 9 254 1302 1433 2042 3195
Size: 9 245 1048 131 609 1153
Fragments arranged by size:
1153 1048 609 245 131 9
BsrBI CCG'CTC
Cuts at: 0 525 785 3195
Size: 525 260 2410
Fragments arranged by size:
2410 525 260
BsrDI GCAATG_nn'
Cuts at: 0 1863 3195
Size: 1863 1332
BsrFI r'CCGG_y
Cuts at: 0 677 1388 2027 2272 3195
Size: 677 711 639 245 923
Fragments arranged by size:
923 711 677 639 245
BsrGI T'GTAC_A
Cuts at: 0 598 3195
Size: 598 2597
BssSI C'ACGA_G
Cuts at: 0 2229 3195
Size: 2229 966
BstEII G'GTnAC_C
Cuts at: 0 1704 3195
Size: 1704 1491
BstXI CCAn_nnnn'nTGG
Cuts at: 0 943 1392 2976 3015 3195
Size: 943 449 1584 39 180
Fragments arranged by size:
1584 943 449 180 39
BstYI r'GATC_y
Cuts at: 0 725 3195
Size: 725 2470
Bsu36I CC'TnA_GG
Cuts at: 0 421 1797 2639 3195
Size: 421 1376 842 556
Fragments arranged by size:
1376 842 556 421
BtgI C'CryG_G
Cuts at: 0 367 778 1325 2417 3195
Size: 367 411 547 1092 778
Fragments arranged by size:
1092 778 547 411 367
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3105 3195
Size: 3105 90
BtsI GCAGTG_nn'
Cuts at: 0 571 1120 2062 2766 3195
Size: 571 549 942 704 429
Fragments arranged by size:
942 704 571 549 429
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2083 2118 3195
Size: 2083 35 1077
Fragments arranged by size:
2083 1077 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1102 2437 3195
Size: 1102 1335 758
Fragments arranged by size:
1335 1102 758
EaeI y'GGCC_r
Cuts at: 0 675 1023 1451 1518 2194 2240 3132 3195
Size: 675 348 428 67 676 46 892 63
Fragments arranged by size:
892 676 675 428 348 67 63 46
EagI C'GGCC_G
Cuts at: 0 675 1518 2194 3195
Size: 675 843 676 1001
Fragments arranged by size:
1001 843 676 675
EarI CTCTTCn'nnn_
Cuts at: 0 308 1930 2133 2902 3195
Size: 308 1622 203 769 293
Fragments arranged by size:
1622 769 308 293 203
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 13 255 1325 1948 3195
Size: 13 242 1070 623 1247
Fragments arranged by size:
1247 1070 623 242 13
EcoICRI GAG'CTC
Cuts at: 0 842 1050 1305 2573 3195
Size: 842 208 255 1268 622
Fragments arranged by size:
1268 842 622 255 208
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 298 438 1060 1291 1516 1609 1827
Size: 118 180 140 622 231 225 93 218
Cuts at: 1827 2327 2518 2559 2726 3195
Size: 500 191 41 167 469
Fragments arranged by size:
622 500 469 231 225 218 191 180
167 140 118 93 41
EcoNI CCTnn'n_nnAGG
Cuts at: 0 971 1656 3195
Size: 971 685 1539
Fragments arranged by size:
1539 971 685
EcoO109I rG'GnC_Cy
Cuts at: 0 273 1004 1095 1330 1364 1377 1513 1527
Size: 273 731 91 235 34 13 136 14
Cuts at: 1527 2211 2526 3011 3195
Size: 684 315 485 184
Fragments arranged by size:
731 684 485 315 273 235 184 136
91 34 14 13
EcoRI G'AATT_C
Cuts at: 0 466 3195
Size: 466 2729
FspI TGC'GCA
Cuts at: 0 148 3195
Size: 148 3047
HaeII r_GCGC'y
Cuts at: 0 262 490 869 1572 3195
Size: 262 228 379 703 1623
Fragments arranged by size:
1623 703 379 262 228
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 529 561 1036 1068 1663 1695 2305 2337
Size: 529 32 475 32 595 32 610 32
Cuts at: 2337 2635 2667 2805 2837 2971 3003 3195
Size: 298 32 138 32 134 32 192
Fragments arranged by size:
610 595 529 475 298 192 138 134
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1611 3195
Size: 1611 1584
HindIII A'AGCT_T
Cuts at: 0 2889 3195
Size: 2889 306
KpnI G_GTAC'C
Cuts at: 0 2847 3195
Size: 2847 348
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1970 2453 2957 3195
Size: 1970 483 504 238
Fragments arranged by size:
1970 504 483 238
MscI TGG'CCA
Cuts at: 0 1025 2242 3195
Size: 1025 1217 953
Fragments arranged by size:
1217 1025 953
MslI CAynn'nnrTG
Cuts at: 0 1981 2396 3195
Size: 1981 415 799
Fragments arranged by size:
1981 799 415
MspA1I CmG'CkG
Cuts at: 0 369 531 612 780 1121 1327 1494 1580
Size: 369 162 81 168 341 206 167 86
Cuts at: 1580 1730 2556 3048 3175 3195
Size: 150 826 492 127 20
Fragments arranged by size:
826 492 369 341 206 168 167 162
150 127 86 81 20
NaeI GCC'GGC
Cuts at: 0 1390 2274 3195
Size: 1390 884 921
Fragments arranged by size:
1390 921 884
NgoMIV G'CCGG_C
Cuts at: 0 1388 2272 3195
Size: 1388 884 923
Fragments arranged by size:
1388 923 884
PasI CC'CwG_GG
Cuts at: 0 1693 3195
Size: 1693 1502
PflMI CCAn_nnn'nTGG
Cuts at: 0 729 3195
Size: 729 2466
PfoI T'CCnGG_A
Cuts at: 0 1307 2284 3195
Size: 1307 977 911
Fragments arranged by size:
1307 977 911
PmlI CAC'GTG
Cuts at: 0 708 3195
Size: 708 2487
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1732 1764 3195
Size: 1732 32 1431
Fragments arranged by size:
1732 1431 32
PpuMI rG'GwC_Cy
Cuts at: 0 1095 1377 1527 2211 3011 3195
Size: 1095 282 150 684 800 184
Fragments arranged by size:
1095 800 684 282 184 150
PspOMI G'GGCC_C
Cuts at: 0 272 386 1513 3195
Size: 272 114 1127 1682
Fragments arranged by size:
1682 1127 272 114
PspXI vC'TCGA_Gb
Cuts at: 0 517 894 3195
Size: 517 377 2301
Fragments arranged by size:
2301 517 377
PstI C_TGCA'G
Cuts at: 0 536 972 1430 1493 3195
Size: 536 436 458 63 1702
Fragments arranged by size:
1702 536 458 436 63
PvuI CG_AT'CG
Cuts at: 0 2201 3195
Size: 2201 994
PvuII CAG'CTG
Cuts at: 0 531 1494 1580 3048 3195
Size: 531 963 86 1468 147
Fragments arranged by size:
1468 963 531 147 86
SacI G_AGCT'C
Cuts at: 0 844 1052 1307 2575 3195
Size: 844 208 255 1268 620
Fragments arranged by size:
1268 844 620 255 208
SacII CC_GC'GG
Cuts at: 0 370 781 1328 3195
Size: 370 411 547 1867
Fragments arranged by size:
1867 547 411 370
SapI GCTCTTCn'nnn_
Cuts at: 0 1930 2902 3195
Size: 1930 972 293
Fragments arranged by size:
1930 972 293
SfcI C'TryA_G
Cuts at: 0 353 361 532 968 1426 1489 1646 3195
Size: 353 8 171 436 458 63 157 1549
Fragments arranged by size:
1549 458 436 353 171 157 63 8
SmaI CCC'GGG
Cuts at: 0 760 873 994 1251 3195
Size: 760 113 121 257 1944
Fragments arranged by size:
1944 760 257 121 113
SmlI C'TyrA_G
Cuts at: 0 89 303 517 894 955 3195
Size: 89 214 214 377 61 2240
Fragments arranged by size:
2240 377 214 214 89 61
StuI AGG'CCT
Cuts at: 0 95 646 921 1747 1874 2457 3195
Size: 95 551 275 826 127 583 738
Fragments arranged by size:
826 738 583 551 275 127 95
StyI C'CwwG_G
Cuts at: 0 641 695 2207 2303 2522 3195
Size: 641 54 1512 96 219 673
Fragments arranged by size:
1512 673 641 219 96 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1917 2842 3195
Size: 1917 925 353
Fragments arranged by size:
1917 925 353
TatI w'GTAC_w
Cuts at: 0 62 598 3195
Size: 62 536 2597
Fragments arranged by size:
2597 536 62
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2036 2068 2931 2963 3195
Size: 2036 32 863 32 232
Fragments arranged by size:
2036 863 232 32 32
XbaI T'CTAG_A
Cuts at: 0 1059 3195
Size: 1059 2136
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1623 2235 3195
Size: 1623 612 960
Fragments arranged by size:
1623 960 612
XhoI C'TCGA_G
Cuts at: 0 89 517 894 3195
Size: 89 428 377 2301
Fragments arranged by size:
2301 428 377 89
XmaI C'CCGG_G
Cuts at: 0 758 871 992 1249 3195
Size: 758 113 121 257 1946
Fragments arranged by size:
1946 758 257 121 113
XmnI GAAnn'nnTTC
Cuts at: 0 203 1924 3195
Size: 203 1721 1271
Fragments arranged by size:
1721 1271 203
ZraI GAC'GTC
Cuts at: 0 2601 3195
Size: 2601 594
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AlfI AloI
AlwNI ApaI ApaLI ApoI AvaI BaeI BanI BanII
BbsI BbvCI BcgI BclI BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
KpnI MmeI MscI MslI MspA1I NaeI NgoMIV PasI
PflMI PfoI PmlI PpiI PpuMI PspOMI PspXI PstI
PvuI PvuII SacI SacII SapI SfcI SmaI SmlI
StuI StyI TaqII TatI TstI XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AflIII AgeI AhdI AleI AscI AseI
AsiSI AvrII BamHI BbeI BciVI BfrBI BglI BglII
BmgBI BmtI BsiWI BssHII BstAPI BstBI BstZ17I ClaI
DraI DraIII EcoRV FalI FseI FspAI HpaI KasI
MfeI MluI NarI NcoI NdeI NheI NotI NruI
NsiI NspI PacI PciI PmeI PshAI PsiI PsrI
RsrII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I