Restriction map for
pF1KA1432
(Linear) (Six-base) MAPSORT of: KIAA1432.seq Check: 3502 from: 1 to: 2373
KIAA1432.orf
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AccI GT'mk_AC
Cuts at: 0 1029 1415 2373
Size: 1029 386 958
Fragments arranged by size:
1029 958 386
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1119 1456 1994 2373
Size: 1119 337 538 379
Fragments arranged by size:
1119 538 379 337
AflIII A'CryG_T
Cuts at: 0 271 1271 2373
Size: 271 1000 1102
Fragments arranged by size:
1102 1000 271
AhdI GACnn_n'nnGTC
Cuts at: 0 272 2373
Size: 272 2101
AlwNI CAG_nnn'CTG
Cuts at: 0 1259 2373
Size: 1259 1114
ApoI r'AATT_y
Cuts at: 0 769 1189 1803 2373
Size: 769 420 614 570
Fragments arranged by size:
769 614 570 420
AvaI C'yCGr_G
Cuts at: 0 1400 1409 2219 2373
Size: 1400 9 810 154
Fragments arranged by size:
1400 810 154 9
AvrII C'CTAG_G
Cuts at: 0 364 2373
Size: 364 2009
BanI G'GyrC_C
Cuts at: 0 1307 1928 2373
Size: 1307 621 445
Fragments arranged by size:
1307 621 445
BanII G_rGCy'C
Cuts at: 0 692 737 2054 2242 2373
Size: 692 45 1317 188 131
Fragments arranged by size:
1317 692 188 131 45
BbeI G_GCGC'C
Cuts at: 0 1311 2373
Size: 1311 1062
BbsI GAAGACnn'nnnn_
Cuts at: 0 829 2000 2373
Size: 829 1171 373
Fragments arranged by size:
1171 829 373
BglII A'GATC_T
Cuts at: 0 578 2373
Size: 578 1795
BlpI GC'TnA_GC
Cuts at: 0 319 1624 2373
Size: 319 1305 749
Fragments arranged by size:
1305 749 319
BmrI ACTGGGnnnn_n'
Cuts at: 0 240 642 1103 1155 2373
Size: 240 402 461 52 1218
Fragments arranged by size:
1218 461 402 240 52
BmtI G_CTAG'C
Cuts at: 0 442 2373
Size: 442 1931
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 588 590 1319 2373
Size: 588 2 729 1054
Fragments arranged by size:
1054 729 588 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 556 706 1085 1314 1858 2186 2229
Size: 325 231 150 379 229 544 328 43
Cuts at: 2229 2373
Size: 144
Fragments arranged by size:
544 379 328 325 231 229 150 144
43
Bpu10I CC'TnA_GC
Cuts at: 0 902 1100 2373
Size: 902 198 1273
Fragments arranged by size:
1273 902 198
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1597 2373
Size: 1597 776
BsaHI Gr'CG_yC
Cuts at: 0 1308 2373
Size: 1308 1065
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1912 1942 2373
Size: 1912 30 431
Fragments arranged by size:
1912 431 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 901 2232 2242 2320 2341 2373
Size: 901 1331 10 78 21 32
Fragments arranged by size:
1331 901 78 32 21 10
BseYI C'CCAG_C
Cuts at: 0 398 1355 1359 1479 2373
Size: 398 957 4 120 894
Fragments arranged by size:
957 894 398 120 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 353 2373
Size: 125 228 2020
Fragments arranged by size:
2020 228 125
BsiHKAI G_wGCw'C
Cuts at: 0 545 692 1844 2373
Size: 545 147 1152 529
Fragments arranged by size:
1152 545 529 147
BsmI GAATG_Cn'
Cuts at: 0 1732 2373
Size: 1732 641
Bsp1286I G_dGCh'C
Cuts at: 0 545 692 737 1844 2054 2242 2373
Size: 545 147 45 1107 210 188 131
Fragments arranged by size:
1107 545 210 188 147 131 45
BspHI T'CATG_A
Cuts at: 0 143 1647 2373
Size: 143 1504 726
Fragments arranged by size:
1504 726 143
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2148 2373
Size: 2148 225
BssSI C'ACGA_G
Cuts at: 0 98 2373
Size: 98 2275
BstAPI GCAn_nnn'nTGC
Cuts at: 0 636 2373
Size: 636 1737
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1857 2373
Size: 1857 516
BstYI r'GATC_y
Cuts at: 0 173 578 2373
Size: 173 405 1795
Fragments arranged by size:
1795 405 173
Bsu36I CC'TnA_GG
Cuts at: 0 872 2373
Size: 872 1501
BtgI C'CryG_G
Cuts at: 0 1671 1769 1850 2250 2373
Size: 1671 98 81 400 123
Fragments arranged by size:
1671 400 123 98 81
BtsI GCAGTG_nn'
Cuts at: 0 2066 2373
Size: 2066 307
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 997 1032 2373
Size: 997 35 1341
Fragments arranged by size:
1341 997 35
DraI TTT'AAA
Cuts at: 0 2108 2373
Size: 2108 265
EaeI y'GGCC_r
Cuts at: 0 1462 1859 2373
Size: 1462 397 514
Fragments arranged by size:
1462 514 397
EarI CTCTTCn'nnn_
Cuts at: 0 364 550 796 1432 1491 2147 2373
Size: 364 186 246 636 59 656 226
Fragments arranged by size:
656 636 364 246 226 186 59
EcoICRI GAG'CTC
Cuts at: 0 690 2373
Size: 690 1683
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 556 706 1085 1119 1314 1456 1858
Size: 325 231 150 379 34 195 142 402
Cuts at: 1858 1994 2186 2229 2373
Size: 136 192 43 144
Fragments arranged by size:
402 379 325 231 195 192 150 144
142 136 43 34
EcoNI CCTnn'n_nnAGG
Cuts at: 0 304 2373
Size: 304 2069
EcoO109I rG'GnC_Cy
Cuts at: 0 309 1020 1335 1870 2373
Size: 309 711 315 535 503
Fragments arranged by size:
711 535 503 315 309
HaeII r_GCGC'y
Cuts at: 0 1311 2373
Size: 1311 1062
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 125 157 258 290 2373
Size: 125 32 101 32 2083
Fragments arranged by size:
2083 125 101 32 32
HincII GTy'rAC
Cuts at: 0 604 1030 2373
Size: 604 426 1343
Fragments arranged by size:
1343 604 426
KasI G'GCGC_C
Cuts at: 0 1307 2373
Size: 1307 1066
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 843 1622 2202 2256 2373
Size: 843 779 580 54 117
Fragments arranged by size:
843 779 580 117 54
MscI TGG'CCA
Cuts at: 0 1464 1861 2373
Size: 1464 397 512
Fragments arranged by size:
1464 512 397
MslI CAynn'nnrTG
Cuts at: 0 145 1849 1915 2373
Size: 145 1704 66 458
Fragments arranged by size:
1704 458 145 66
MspA1I CmG'CkG
Cuts at: 0 402 534 859 919 1168 1359 1539 1558
Size: 402 132 325 60 249 191 180 19
Cuts at: 1558 2373
Size: 815
Fragments arranged by size:
815 402 325 249 191 180 132 60
19
NarI GG'CG_CC
Cuts at: 0 1308 2373
Size: 1308 1065
NcoI C'CATG_G
Cuts at: 0 1671 1769 1850 2250 2373
Size: 1671 98 81 400 123
Fragments arranged by size:
1671 400 123 98 81
NheI G'CTAG_C
Cuts at: 0 438 2373
Size: 438 1935
NspI r_CATG'y
Cuts at: 0 979 1275 1303 2373
Size: 979 296 28 1070
Fragments arranged by size:
1070 979 296 28
PasI CC'CwG_GG
Cuts at: 0 2265 2373
Size: 2265 108
PciI A'CATG_T
Cuts at: 0 1271 2373
Size: 1271 1102
PflMI CCAn_nnn'nTGG
Cuts at: 0 2061 2373
Size: 2061 312
PfoI T'CCnGG_A
Cuts at: 0 176 2088 2373
Size: 176 1912 285
Fragments arranged by size:
1912 285 176
PpuMI rG'GwC_Cy
Cuts at: 0 1020 1335 2373
Size: 1020 315 1038
Fragments arranged by size:
1038 1020 315
PshAI GACnn'nnGTC
Cuts at: 0 805 1025 2373
Size: 805 220 1348
Fragments arranged by size:
1348 805 220
PstI C_TGCA'G
Cuts at: 0 800 963 2132 2373
Size: 800 163 1169 241
Fragments arranged by size:
1169 800 241 163
PvuII CAG'CTG
Cuts at: 0 534 859 919 1168 1359 1539 1558 2373
Size: 534 325 60 249 191 180 19 815
Fragments arranged by size:
815 534 325 249 191 180 60 19
SacI G_AGCT'C
Cuts at: 0 692 2373
Size: 692 1681
SalI G'TCGA_C
Cuts at: 0 1028 2373
Size: 1028 1345
SapI GCTCTTCn'nnn_
Cuts at: 0 550 2147 2373
Size: 550 1597 226
Fragments arranged by size:
1597 550 226
ScaI AGT'ACT
Cuts at: 0 707 2373
Size: 707 1666
SfcI C'TryA_G
Cuts at: 0 796 959 2078 2128 2373
Size: 796 163 1119 50 245
Fragments arranged by size:
1119 796 245 163 50
SfoI GGC'GCC
Cuts at: 0 1309 2373
Size: 1309 1064
SmaI CCC'GGG
Cuts at: 0 1411 2221 2373
Size: 1411 810 152
Fragments arranged by size:
1411 810 152
SmlI C'TyrA_G
Cuts at: 0 1576 2373
Size: 1576 797
StuI AGG'CCT
Cuts at: 0 1955 2373
Size: 1955 418
StyI C'CwwG_G
Cuts at: 0 364 468 621 1338 1671 1769 1850 2250
Size: 364 104 153 717 333 98 81 400
Cuts at: 2250 2373
Size: 123
Fragments arranged by size:
717 400 364 333 153 123 104 98
81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 841 1107 1657 2081 2373
Size: 841 266 550 424 292
Fragments arranged by size:
841 550 424 292 266
TatI w'GTAC_w
Cuts at: 0 58 237 705 2373
Size: 58 179 468 1668
Fragments arranged by size:
1668 468 179 58
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1912 1944 2373
Size: 1912 32 429
Fragments arranged by size:
1912 429 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2272 2373
Size: 2272 101
XmaI C'CCGG_G
Cuts at: 0 1409 2219 2373
Size: 1409 810 154
Fragments arranged by size:
1409 810 154
XmnI GAAnn'nnTTC
Cuts at: 0 1175 2339 2373
Size: 1175 1164 34
Fragments arranged by size:
1175 1164 34
Enzymes that do cut:
AccI AcuI AflIII AhdI AlwNI ApoI AvaI AvrII
BanI BanII BbeI BbsI BglII BlpI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BssSI BstAPI
BstXI BstYI Bsu36I BtgI BtsI CspCI DraI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII
KasI MmeI MscI MslI MspA1I NarI NcoI NheI
NspI PasI PciI PflMI PfoI PpuMI PshAI PstI
PvuII SacI SalI SapI ScaI SfcI SfoI SmaI
SmlI StuI StyI TaqII TatI TstI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI BaeI
BamHI BbvCI BcgI BciVI BclI BfrBI BglI Bme1580I
BmgBI BsaI BsaAI BsaBI BsaWI BsiEI BsiWI BsmBI
BspEI BsrBI BsrDI BsrFI BsrGI BssHII BstBI BstEII
BstZ17I BtgZI ClaI DraIII DrdI EagI EciI EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI
MfeI MluI NaeI NdeI NgoMIV NotI NruI NsiI
PacI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SanDI SbfI SexAI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
XbaI XhoI ZraI