Restriction map for
pF1KA1435
(Linear) (Six-base) MAPSORT of: KIAA1435.seq Check: 395 from: 1 to: 1230
KIAA1435.orf
With 182 enzymes: SgfI *
July 31, 2008 09:34 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 314 348 565 697 736 1230
Size: 314 34 217 132 39 494
Fragments arranged by size:
494 314 217 132 39 34
AfeI AGC'GCT
Cuts at: 0 985 1230
Size: 985 245
AflIII A'CryG_T
Cuts at: 0 560 562 1112 1230
Size: 560 2 550 118
Fragments arranged by size:
560 550 118 2
AlwNI CAG_nnn'CTG
Cuts at: 0 741 1230
Size: 741 489
ApaLI G'TGCA_C
Cuts at: 0 423 1230
Size: 423 807
ApoI r'AATT_y
Cuts at: 0 280 1230
Size: 280 950
AvaI C'yCGr_G
Cuts at: 0 445 1230
Size: 445 785
BanI G'GyrC_C
Cuts at: 0 65 1230
Size: 65 1165
BclI T'GATC_A
Cuts at: 0 116 386 1230
Size: 116 270 844
Fragments arranged by size:
844 270 116
BglI GCCn_nnn'nGGC
Cuts at: 0 82 1230
Size: 82 1148
BglII A'GATC_T
Cuts at: 0 1172 1230
Size: 1172 58
BlpI GC'TnA_GC
Cuts at: 0 49 1230
Size: 49 1181
Bme1580I G_kGCm'C
Cuts at: 0 68 427 1230
Size: 68 359 803
Fragments arranged by size:
803 359 68
BmgBI CAC'GTC
Cuts at: 0 464 1230
Size: 464 766
BmrI ACTGGGnnnn_n'
Cuts at: 0 991 1223 1230
Size: 991 232 7
Fragments arranged by size:
991 232 7
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3 1230
Size: 3 1227
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 972 1230
Size: 972 258
BsaAI yAC'GTr
Cuts at: 0 563 1230
Size: 563 667
BsaBI GATnn'nnATC
Cuts at: 0 1061 1230
Size: 1061 169
BsaHI Gr'CG_yC
Cuts at: 0 74 1230
Size: 74 1156
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 824 1230
Size: 824 406
BseYI C'CCAG_C
Cuts at: 0 181 330 918 1230
Size: 181 149 588 312
Fragments arranged by size:
588 312 181 149
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 754 993 1230
Size: 754 239 237
Fragments arranged by size:
754 239 237
BsiEI CG_ry'CG
Cuts at: 0 8 86 1161 1230
Size: 8 78 1075 69
Fragments arranged by size:
1075 78 69 8
BsiHKAI G_wGCw'C
Cuts at: 0 427 1230
Size: 427 803
BsmBI CGTCTCn'nnnn_
Cuts at: 0 777 1230
Size: 777 453
Bsp1286I G_dGCh'C
Cuts at: 0 68 427 1230
Size: 68 359 803
Fragments arranged by size:
803 359 68
BspHI T'CATG_A
Cuts at: 0 231 723 1086 1230
Size: 231 492 363 144
Fragments arranged by size:
492 363 231 144
BsrBI CCG'CTC
Cuts at: 0 434 1230
Size: 434 796
BsrDI GCAATG_nn'
Cuts at: 0 1124 1230
Size: 1124 106
BsrFI r'CCGG_y
Cuts at: 0 394 1230
Size: 394 836
BstAPI GCAn_nnn'nTGC
Cuts at: 0 947 1230
Size: 947 283
BstEII G'GTnAC_C
Cuts at: 0 1143 1230
Size: 1143 87
BstXI CCAn_nnnn'nTGG
Cuts at: 0 265 884 918 1230
Size: 265 619 34 312
Fragments arranged by size:
619 312 265 34
BstYI r'GATC_y
Cuts at: 0 1172 1230
Size: 1172 58
BtgI C'CryG_G
Cuts at: 0 1118 1230
Size: 1118 112
BtsI GCAGTG_nn'
Cuts at: 0 806 936 1230
Size: 806 130 294
Fragments arranged by size:
806 294 130
DraIII CAC_nnn'GTG
Cuts at: 0 380 1230
Size: 380 850
EaeI y'GGCC_r
Cuts at: 0 5 83 122 371 396 1230
Size: 5 78 39 249 25 834
Fragments arranged by size:
834 249 78 39 25 5
EagI C'GGCC_G
Cuts at: 0 5 83 1230
Size: 5 78 1147
Fragments arranged by size:
1147 78 5
EarI CTCTTCn'nnn_
Cuts at: 0 646 821 1230
Size: 646 175 409
Fragments arranged by size:
646 409 175
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 806 1230
Size: 806 424
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3 314 348 565 697 736 1230
Size: 3 311 34 217 132 39 494
Fragments arranged by size:
494 311 217 132 39 34 3
EcoO109I rG'GnC_Cy
Cuts at: 0 621 1230
Size: 621 609
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 872 1230
Size: 872 358
HaeII r_GCGC'y
Cuts at: 0 987 1230
Size: 987 243
HindIII A'AGCT_T
Cuts at: 0 547 1230
Size: 547 683
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 824 1230
Size: 824 406
MscI TGG'CCA
Cuts at: 0 373 1230
Size: 373 857
MslI CAynn'nnrTG
Cuts at: 0 670 1117 1230
Size: 670 447 113
Fragments arranged by size:
670 447 113
MspA1I CmG'CkG
Cuts at: 0 633 1230
Size: 633 597
NcoI C'CATG_G
Cuts at: 0 1118 1230
Size: 1118 112
NspI r_CATG'y
Cuts at: 0 444 1116 1230
Size: 444 672 114
Fragments arranged by size:
672 444 114
PciI A'CATG_T
Cuts at: 0 1112 1230
Size: 1112 118
PflMI CCAn_nnn'nTGG
Cuts at: 0 198 380 1230
Size: 198 182 850
Fragments arranged by size:
850 198 182
PmlI CAC'GTG
Cuts at: 0 563 1230
Size: 563 667
PpuMI rG'GwC_Cy
Cuts at: 0 621 1230
Size: 621 609
PshAI GACnn'nnGTC
Cuts at: 0 167 1230
Size: 167 1063
PstI C_TGCA'G
Cuts at: 0 943 1204 1230
Size: 943 261 26
Fragments arranged by size:
943 261 26
SanDI GG'GwC_CC
Cuts at: 0 621 1230
Size: 621 609
SfcI C'TryA_G
Cuts at: 0 939 1200 1230
Size: 939 261 30
Fragments arranged by size:
939 261 30
SmlI C'TyrA_G
Cuts at: 0 987 1230
Size: 987 243
StyI C'CwwG_G
Cuts at: 0 101 1118 1230
Size: 101 1017 112
Fragments arranged by size:
1017 112 101
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 372 1171 1230
Size: 372 799 59
Fragments arranged by size:
799 372 59
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 785 817 1127 1159 1230
Size: 785 32 310 32 71
Fragments arranged by size:
785 310 71 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 76 1230
Size: 76 1154
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1137 1230
Size: 1137 93
Enzymes that do cut:
AcuI AfeI AflIII AlwNI ApaLI ApoI AvaI BanI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI
BpuEI BsaAI BsaBI BsaHI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspHI BsrBI BsrDI BsrFI BstAPI
BstEII BstXI BstYI BtgI BtsI DraIII EaeI EagI
EarI EciI Eco57MI EcoO109I FalI HaeII HindIII MmeI
MscI MslI MspA1I NcoI NspI PciI PflMI PmlI
PpuMI PshAI PstI SanDI SfcI SmlI StyI TaqII
TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AleI AlfI AloI ApaI AscI AseI AsiSI AvrII
BaeI BamHI BanII BbeI BbsI BbvCI BcgI BciVI
BfrBI BmtI BplI Bpu10I BsaI BsaWI BsaXI BsiWI
BsmI BspEI BspMI BsrGI BssHII BssSI BstBI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DrdI EcoICRI EcoNI
EcoRI EcoRV FseI FspI FspAI Hin4I HincII HpaI
KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PfoI PmeI
PpiI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI XbaI XhoI XmaI XmnI
ZraI