Restriction map for
pF1KSDA1436
(Linear) (Six-base) MAPSORT of: KIAA1436.seq Check: 2349 from: 1 to: 2637
KIAA1436.orf
With 182 enzymes: SgfI *
February 28, 2007 11:29 ..
AatII G_ACGT'C
Cuts at: 0 926 2637
Size: 926 1711
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 890 933 2637
Size: 317 573 43 1704
Fragments arranged by size:
1704 573 317 43
AfeI AGC'GCT
Cuts at: 0 640 2637
Size: 640 1997
AflIII A'CryG_T
Cuts at: 0 1121 1487 2637
Size: 1121 366 1150
Fragments arranged by size:
1150 1121 366
AleI CACnn'nnGTG
Cuts at: 0 814 2637
Size: 814 1823
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 214 246 1894 1926 2637
Size: 214 32 1648 32 711
Fragments arranged by size:
1648 711 214 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1154 2637
Size: 1154 1483
ApaI G_GGCC'C
Cuts at: 0 445 1449 2637
Size: 445 1004 1188
Fragments arranged by size:
1188 1004 445
ApaLI G'TGCA_C
Cuts at: 0 559 1018 2200 2637
Size: 559 459 1182 437
Fragments arranged by size:
1182 559 459 437
ApoI r'AATT_y
Cuts at: 0 2323 2637
Size: 2323 314
AvaI C'yCGr_G
Cuts at: 0 15 618 704 931 1252 2637
Size: 15 603 86 227 321 1385
Fragments arranged by size:
1385 603 321 227 86 15
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 723 756 2637
Size: 723 33 1881
Fragments arranged by size:
1881 723 33
BamHI G'GATC_C
Cuts at: 0 1502 2160 2637
Size: 1502 658 477
Fragments arranged by size:
1502 658 477
BanI G'GyrC_C
Cuts at: 0 5 1134 2637
Size: 5 1129 1503
Fragments arranged by size:
1503 1129 5
BanII G_rGCy'C
Cuts at: 0 311 445 491 711 730 1449 2534 2637
Size: 311 134 46 220 19 719 1085 103
Fragments arranged by size:
1085 719 311 220 134 103 46 19
BbeI G_GCGC'C
Cuts at: 0 9 2637
Size: 9 2628
BbsI GAAGACnn'nnnn_
Cuts at: 0 1172 1529 1593 2231 2637
Size: 1172 357 64 638 406
Fragments arranged by size:
1172 638 406 357 64
BbvCI CC'TCA_GC
Cuts at: 0 2296 2637
Size: 2296 341
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 6 40 1372 1406 2637
Size: 6 34 1332 34 1231
Fragments arranged by size:
1332 1231 34 34 6
BciVI GTATCCnnnnn_n'
Cuts at: 0 2501 2637
Size: 2501 136
BclI T'GATC_A
Cuts at: 0 2117 2637
Size: 2117 520
BfrBI ATG'CAT
Cuts at: 0 1864 2637
Size: 1864 773
BglII A'GATC_T
Cuts at: 0 2112 2637
Size: 2112 525
Bme1580I G_kGCm'C
Cuts at: 0 109 445 563 851 1022 1137 1449 2204
Size: 109 336 118 288 171 115 312 755
Cuts at: 2204 2637
Size: 433
Fragments arranged by size:
755 433 336 312 288 171 118 115
109
BmgBI CAC'GTC
Cuts at: 0 657 941 2637
Size: 657 284 1696
Fragments arranged by size:
1696 657 284
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2454 2637
Size: 2454 183
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 192 1773 1867 2323 2637
Size: 192 1581 94 456 314
Fragments arranged by size:
1581 456 314 192 94
Bpu10I CC'TnA_GC
Cuts at: 0 469 2296 2637
Size: 469 1827 341
Fragments arranged by size:
1827 469 341
BsaI GGTCTCn'nnnn_
Cuts at: 0 1944 1985 2637
Size: 1944 41 652
Fragments arranged by size:
1944 652 41
BsaAI yAC'GTr
Cuts at: 0 438 1122 2637
Size: 438 684 1515
Fragments arranged by size:
1515 684 438
BsaHI Gr'CG_yC
Cuts at: 0 6 299 683 923 2507 2637
Size: 6 293 384 240 1584 130
Fragments arranged by size:
1584 384 293 240 130 6
BsaWI w'CCGG_w
Cuts at: 0 1499 2637
Size: 1499 1138
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 214 244 712 742 1894 1924 2545 2575
Size: 214 30 468 30 1152 30 621 30
Cuts at: 2575 2637
Size: 62
Fragments arranged by size:
1152 621 468 214 62 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1446 2637
Size: 1446 1191
BseYI C'CCAG_C
Cuts at: 0 151 234 239 364 445 1185 1580 2637
Size: 151 83 5 125 81 740 395 1057
Fragments arranged by size:
1057 740 395 151 125 83 81 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 427 489 514 1918 2637
Size: 418 9 62 25 1404 719
Fragments arranged by size:
1404 719 418 62 25 9
BsiHKAI G_wGCw'C
Cuts at: 0 311 563 1022 2204 2234 2637
Size: 311 252 459 1182 30 403
Fragments arranged by size:
1182 459 403 311 252 30
BsmI GAATG_Cn'
Cuts at: 0 1242 2082 2637
Size: 1242 840 555
Fragments arranged by size:
1242 840 555
Bsp1286I G_dGCh'C
Cuts at: 0 109 311 445 491 563 711 730 851
Size: 109 202 134 46 72 148 19 121
Cuts at: 851 1022 1137 1449 2204 2234 2534 2637
Size: 171 115 312 755 30 300 103
Fragments arranged by size:
755 312 300 202 171 148 134 121
115 109 103 72 46 30 19
BspEI T'CCGG_A
Cuts at: 0 1499 2637
Size: 1499 1138
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 399 599 2637
Size: 399 200 2038
Fragments arranged by size:
2038 399 200
BsrBI CCG'CTC
Cuts at: 0 255 2637
Size: 255 2382
BsrFI r'CCGG_y
Cuts at: 0 1355 2637
Size: 1355 1282
BssHII G'CGCG_C
Cuts at: 0 449 2637
Size: 449 2188
BssSI C'ACGA_G
Cuts at: 0 601 2637
Size: 601 2036
BstEII G'GTnAC_C
Cuts at: 0 1374 2637
Size: 1374 1263
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1278 2362 2637
Size: 1278 1084 275
Fragments arranged by size:
1278 1084 275
BstYI r'GATC_y
Cuts at: 0 272 1502 2112 2160 2432 2637
Size: 272 1230 610 48 272 205
Fragments arranged by size:
1230 610 272 272 205 48
Bsu36I CC'TnA_GG
Cuts at: 0 2055 2637
Size: 2055 582
BtgI C'CryG_G
Cuts at: 0 302 564 671 797 812 2389 2519 2637
Size: 302 262 107 126 15 1577 130 118
Fragments arranged by size:
1577 302 262 130 126 118 107 15
BtsI GCAGTG_nn'
Cuts at: 0 506 2637
Size: 506 2131
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2554 2589 2637
Size: 2554 35 48
Fragments arranged by size:
2554 48 35
DraIII CAC_nnn'GTG
Cuts at: 0 1164 2637
Size: 1164 1473
DrdI GACnn_nn'nnGTC
Cuts at: 0 1977 2637
Size: 1977 660
EaeI y'GGCC_r
Cuts at: 0 422 572 2637
Size: 422 150 2065
Fragments arranged by size:
2065 422 150
EarI CTCTTCn'nnn_
Cuts at: 0 1297 2281 2637
Size: 1297 984 356
Fragments arranged by size:
1297 984 356
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 300 507 594 2637
Size: 300 207 87 2043
Fragments arranged by size:
2043 300 207 87
EcoICRI GAG'CTC
Cuts at: 0 309 2637
Size: 309 2328
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 192 317 890 933 1773 1867 2323 2637
Size: 192 125 573 43 840 94 456 314
Fragments arranged by size:
840 573 456 314 192 125 94 43
EcoNI CCTnn'n_nnAGG
Cuts at: 0 478 951 2218 2274 2637
Size: 478 473 1267 56 363
Fragments arranged by size:
1267 478 473 363 56
EcoO109I rG'GnC_Cy
Cuts at: 0 442 1248 2059 2637
Size: 442 806 811 578
Fragments arranged by size:
811 806 578 442
EcoRI G'AATT_C
Cuts at: 0 2323 2637
Size: 2323 314
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1617 1695 2511 2637
Size: 1617 78 816 126
Fragments arranged by size:
1617 816 126 78
HaeII r_GCGC'y
Cuts at: 0 9 37 547 642 2610 2637
Size: 9 28 510 95 1968 27
Fragments arranged by size:
1968 510 95 28 27 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 712 744 1045 1077 1162 1194 1588 1620
Size: 712 32 301 32 85 32 394 32
Cuts at: 1620 2080 2102 2112 2134 2637
Size: 460 22 10 22 503
Fragments arranged by size:
712 503 460 394 301 85 32 32
32 32 22 22 10
HincII GTy'rAC
Cuts at: 0 1617 2637
Size: 1617 1020
HpaI GTT'AAC
Cuts at: 0 1617 2637
Size: 1617 1020
KasI G'GCGC_C
Cuts at: 0 5 2637
Size: 5 2632
MslI CAynn'nnrTG
Cuts at: 0 814 1052 2623 2637
Size: 814 238 1571 14
Fragments arranged by size:
1571 814 238 14
MspA1I CmG'CkG
Cuts at: 0 24 243 528 566 844 1189 1443 1569
Size: 24 219 285 38 278 345 254 126
Cuts at: 1569 1580 1961 2637
Size: 11 381 676
Fragments arranged by size:
676 381 345 285 278 254 219 126
38 24 11
NarI GG'CG_CC
Cuts at: 0 6 2637
Size: 6 2631
NcoI C'CATG_G
Cuts at: 0 2389 2637
Size: 2389 248
NsiI A_TGCA'T
Cuts at: 0 1866 2637
Size: 1866 771
NspI r_CATG'y
Cuts at: 0 1286 2637
Size: 1286 1351
PflMI CCAn_nnn'nTGG
Cuts at: 0 1074 2637
Size: 1074 1563
PfoI T'CCnGG_A
Cuts at: 0 2272 2637
Size: 2272 365
PmlI CAC'GTG
Cuts at: 0 438 1122 2637
Size: 438 684 1515
Fragments arranged by size:
1515 684 438
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 214 246 595 627 1894 1926 2637
Size: 214 32 349 32 1267 32 711
Fragments arranged by size:
1267 711 349 214 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1248 2059 2637
Size: 1248 811 578
Fragments arranged by size:
1248 811 578
PshAI GACnn'nnGTC
Cuts at: 0 1936 2637
Size: 1936 701
PsiI TTA'TAA
Cuts at: 0 2455 2637
Size: 2455 182
PspOMI G'GGCC_C
Cuts at: 0 441 1445 2637
Size: 441 1004 1192
Fragments arranged by size:
1192 1004 441
PspXI vC'TCGA_Gb
Cuts at: 0 15 2637
Size: 15 2622
PstI C_TGCA'G
Cuts at: 0 263 2560 2637
Size: 263 2297 77
Fragments arranged by size:
2297 263 77
PvuII CAG'CTG
Cuts at: 0 243 844 1189 1580 2637
Size: 243 601 345 391 1057
Fragments arranged by size:
1057 601 391 345 243
SacI G_AGCT'C
Cuts at: 0 311 2637
Size: 311 2326
SacII CC_GC'GG
Cuts at: 0 567 2637
Size: 567 2070
SapI GCTCTTCn'nnn_
Cuts at: 0 2281 2637
Size: 2281 356
SexAI A'CCwGG_T
Cuts at: 0 1223 2637
Size: 1223 1414
SfcI C'TryA_G
Cuts at: 0 259 732 1344 1514 2556 2637
Size: 259 473 612 170 1042 81
Fragments arranged by size:
1042 612 473 259 170 81
SfoI GGC'GCC
Cuts at: 0 7 2637
Size: 7 2630
SmaI CCC'GGG
Cuts at: 0 620 706 1254 2637
Size: 620 86 548 1383
Fragments arranged by size:
1383 620 548 86
SmlI C'TyrA_G
Cuts at: 0 15 2637
Size: 15 2622
SphI G_CATG'C
Cuts at: 0 1286 2637
Size: 1286 1351
SspI AAT'ATT
Cuts at: 0 1731 2637
Size: 1731 906
StyI C'CwwG_G
Cuts at: 0 339 1244 2389 2637
Size: 339 905 1145 248
Fragments arranged by size:
1145 905 339 248
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 926 1186 1572 2382 2500 2637
Size: 926 260 386 810 118 137
Fragments arranged by size:
926 810 386 260 137 118
TatI w'GTAC_w
Cuts at: 0 1808 2637
Size: 1808 829
Tth111I GACn'n_nGTC
Cuts at: 0 942 2637
Size: 942 1695
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 87 300 2411 2637
Size: 87 213 2111 226
Fragments arranged by size:
2111 226 213 87
XhoI C'TCGA_G
Cuts at: 0 15 2637
Size: 15 2622
XmaI C'CCGG_G
Cuts at: 0 618 704 1252 2637
Size: 618 86 548 1385
Fragments arranged by size:
1385 618 548 86
XmnI GAAnn'nnTTC
Cuts at: 0 2482 2637
Size: 2482 155
ZraI GAC'GTC
Cuts at: 0 924 2637
Size: 924 1713
Enzymes that do cut:
AatII AcuI AfeI AflIII AleI AloI AlwNI ApaI
ApaLI ApoI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglII Bme1580I
BmgBI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspMI BsrBI BsrFI BssHII BssSI BstEII BstXI BstYI
Bsu36I BtgI BtsI CspCI DraIII DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII
Hin4I HincII HpaI KasI MslI MspA1I NarI NcoI
NsiI NspI PflMI PfoI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuII SacI SacII SapI
SexAI SfcI SfoI SmaI SmlI SphI SspI StyI
TaqII TatI Tth111I XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AgeI AhdI AlfI
AscI AseI AsiSI AvrII BglI BlpI BmrI BmtI
BpuEI BsaBI BsiEI BsiWI BsmBI BspHI BsrDI BsrGI
BstAPI BstBI BstZ17I BtgZI ClaI DraI EagI EcoRV
FseI FspI FspAI HindIII KpnI MfeI MluI MmeI
MscI NaeI NdeI NgoMIV NheI NotI NruI PacI
PasI PciI PmeI PsrI PvuI RsrII SalI SanDI
SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SrfI
StuI SwaI TstI XbaI