Restriction map for
pF1KSDA1437
(Linear) (Six-base) MAPSORT of: KIAA1437.seq Check: 3283 from: 1 to: 2430
KIAA1437.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1712 1919 1933 2430
Size: 1712 207 14 497
Fragments arranged by size:
1712 497 207 14
AatII G_ACGT'C
Cuts at: 0 1103 2430
Size: 1103 1327
AccI GT'mk_AC
Cuts at: 0 848 1012 2430
Size: 848 164 1418
Fragments arranged by size:
1418 848 164
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 502 543 1564 1815 2430
Size: 79 423 41 1021 251 615
Fragments arranged by size:
1021 615 423 251 79 41
AfeI AGC'GCT
Cuts at: 0 1165 2430
Size: 1165 1265
AflII C'TTAA_G
Cuts at: 0 1930 2430
Size: 1930 500
AhdI GACnn_n'nnGTC
Cuts at: 0 588 2430
Size: 588 1842
AleI CACnn'nnGTG
Cuts at: 0 1020 1690 2430
Size: 1020 670 740
Fragments arranged by size:
1020 740 670
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 377 2239 2430
Size: 377 1862 191
Fragments arranged by size:
1862 377 191
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2353 2385 2430
Size: 2353 32 45
Fragments arranged by size:
2353 45 32
AlwNI CAG_nnn'CTG
Cuts at: 0 223 1713 1911 2235 2430
Size: 223 1490 198 324 195
Fragments arranged by size:
1490 324 223 198 195
ApaI G_GGCC'C
Cuts at: 0 280 516 2205 2430
Size: 280 236 1689 225
Fragments arranged by size:
1689 280 236 225
ApaLI G'TGCA_C
Cuts at: 0 856 1699 2430
Size: 856 843 731
Fragments arranged by size:
856 843 731
ApoI r'AATT_y
Cuts at: 0 433 2430
Size: 433 1997
AvaI C'yCGr_G
Cuts at: 0 2386 2430
Size: 2386 44
BamHI G'GATC_C
Cuts at: 0 53 2430
Size: 53 2377
BanI G'GyrC_C
Cuts at: 0 308 1732 2430
Size: 308 1424 698
Fragments arranged by size:
1424 698 308
BanII G_rGCy'C
Cuts at: 0 20 236 280 516 1628 2180 2205 2430
Size: 20 216 44 236 1112 552 25 225
Fragments arranged by size:
1112 552 236 225 216 44 25 20
BbsI GAAGACnn'nnnn_
Cuts at: 0 111 1167 1552 2430
Size: 111 1056 385 878
Fragments arranged by size:
1056 878 385 111
BbvCI CC'TCA_GC
Cuts at: 0 2074 2430
Size: 2074 356
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2001 2035 2430
Size: 2001 34 395
Fragments arranged by size:
2001 395 34
BclI T'GATC_A
Cuts at: 0 815 2430
Size: 815 1615
BglI GCCn_nnn'nGGC
Cuts at: 0 284 908 2146 2430
Size: 284 624 1238 284
Fragments arranged by size:
1238 624 284 284
Bme1580I G_kGCm'C
Cuts at: 0 280 516 629 860 933 1703 1735 2205
Size: 280 236 113 231 73 770 32 470
Cuts at: 2205 2329 2430
Size: 124 101
Fragments arranged by size:
770 470 280 236 231 124 113 101
73 32
BmgBI CAC'GTC
Cuts at: 0 612 876 1374 2430
Size: 612 264 498 1056
Fragments arranged by size:
1056 612 498 264
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 1303 1363 1378 1573 1807 1828 2011
Size: 478 825 60 15 195 234 21 183
Cuts at: 2011 2113 2226 2317 2430
Size: 102 113 91 113
Fragments arranged by size:
825 478 234 195 183 113 113 102
91 60 21 15
Bpu10I CC'TnA_GC
Cuts at: 0 1579 1663 2074 2430
Size: 1579 84 411 356
Fragments arranged by size:
1579 411 356 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1027 1336 1396 1621 1636 1744 1846 1861
Size: 1027 309 60 225 15 108 102 15
Cuts at: 1861 2320 2430
Size: 459 110
Fragments arranged by size:
1027 459 309 225 110 108 102 60
15 15
BsaAI yAC'GTr
Cuts at: 0 324 855 2430
Size: 324 531 1575
Fragments arranged by size:
1575 531 324
BsaHI Gr'CG_yC
Cuts at: 0 964 1100 2430
Size: 964 136 1330
Fragments arranged by size:
1330 964 136
BsaWI w'CCGG_w
Cuts at: 0 6 50 749 2153 2172 2430
Size: 6 44 699 1404 19 258
Fragments arranged by size:
1404 699 258 44 19 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 232 262 2154 2184 2353 2383 2389 2419
Size: 232 30 1892 30 169 30 6 30
Cuts at: 2419 2430
Size: 11
Fragments arranged by size:
1892 232 169 30 30 30 30 11
6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 550 778 1087 1570 1903 2374 2430
Size: 550 228 309 483 333 471 56
Fragments arranged by size:
550 483 471 333 309 228 56
BseYI C'CCAG_C
Cuts at: 0 212 1387 1397 2036 2316 2430
Size: 212 1175 10 639 280 114
Fragments arranged by size:
1175 639 280 212 114 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 376 867 1111 1273 1477 1546 1897
Size: 337 39 491 244 162 204 69 351
Cuts at: 1897 2191 2430
Size: 294 239
Fragments arranged by size:
491 351 337 294 244 239 204 162
69 39
BsiEI CG_ry'CG
Cuts at: 0 599 2430
Size: 599 1831
BsiHKAI G_wGCw'C
Cuts at: 0 20 464 860 1628 1703 2180 2430
Size: 20 444 396 768 75 477 250
Fragments arranged by size:
768 477 444 396 250 75 20
BsmBI CGTCTCn'nnnn_
Cuts at: 0 676 2155 2430
Size: 676 1479 275
Fragments arranged by size:
1479 676 275
Bsp1286I G_dGCh'C
Cuts at: 0 20 236 280 464 516 629 860 933
Size: 20 216 44 184 52 113 231 73
Cuts at: 933 1628 1703 1735 2180 2205 2329 2430
Size: 695 75 32 445 25 124 101
Fragments arranged by size:
695 445 231 216 184 124 113 101
75 73 52 44 32 25 20
BspEI T'CCGG_A
Cuts at: 0 749 2172 2430
Size: 749 1423 258
Fragments arranged by size:
1423 749 258
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 126 1490 1712 1853 1919 1933 2430
Size: 126 1364 222 141 66 14 497
Fragments arranged by size:
1364 497 222 141 126 66 14
BsrBI CCG'CTC
Cuts at: 0 677 1152 2400 2430
Size: 677 475 1248 30
Fragments arranged by size:
1248 677 475 30
BsrDI GCAATG_nn'
Cuts at: 0 201 1391 2430
Size: 201 1190 1039
Fragments arranged by size:
1190 1039 201
BsrFI r'CCGG_y
Cuts at: 0 305 552 2291 2430
Size: 305 247 1739 139
Fragments arranged by size:
1739 305 247 139
BssSI C'ACGA_G
Cuts at: 0 507 2430
Size: 507 1923
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2235 2430
Size: 2235 195
BstEII G'GTnAC_C
Cuts at: 0 138 177 2430
Size: 138 39 2253
Fragments arranged by size:
2253 138 39
BstYI r'GATC_y
Cuts at: 0 53 956 1520 1532 2027 2430
Size: 53 903 564 12 495 403
Fragments arranged by size:
903 564 495 403 53 12
BstZ17I GTA'TAC
Cuts at: 0 1013 2430
Size: 1013 1417
BtgI C'CryG_G
Cuts at: 0 64 578 887 2430
Size: 64 514 309 1543
Fragments arranged by size:
1543 514 309 64
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1935 2430
Size: 1935 495
BtsI GCAGTG_nn'
Cuts at: 0 2233 2329 2377 2430
Size: 2233 96 48 53
Fragments arranged by size:
2233 96 53 48
DraIII CAC_nnn'GTG
Cuts at: 0 468 2430
Size: 468 1962
EaeI y'GGCC_r
Cuts at: 0 941 1439 2147 2430
Size: 941 498 708 283
Fragments arranged by size:
941 708 498 283
EarI CTCTTCn'nnn_
Cuts at: 0 955 2047 2185 2430
Size: 955 1092 138 245
Fragments arranged by size:
1092 955 245 138
EcoICRI GAG'CTC
Cuts at: 0 18 1626 2178 2430
Size: 18 1608 552 252
Fragments arranged by size:
1608 552 252 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 478 502 543 1303 1363 1378 1564
Size: 79 399 24 41 760 60 15 186
Cuts at: 1564 1573 1807 1815 1828 2011 2113 2226 2317
Size: 9 234 8 13 183 102 113 91
Cuts at: 2317 2430
Size: 113
Fragments arranged by size:
760 399 234 186 183 113 113 102
91 79 60 41 24 15 13 9
8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1183 2430
Size: 1183 1247
EcoO109I rG'GnC_Cy
Cuts at: 0 277 512 513 1347 2202 2364 2430
Size: 277 235 1 834 855 162 66
Fragments arranged by size:
855 834 277 235 162 66 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 573 1128 2430
Size: 573 555 1302
Fragments arranged by size:
1302 573 555
HaeII r_GCGC'y
Cuts at: 0 727 1038 1167 1456 1492 1998 2430
Size: 727 311 129 289 36 506 432
Fragments arranged by size:
727 506 432 311 289 129 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 76 108 574 606 2154 2186 2430
Size: 76 32 466 32 1548 32 244
Fragments arranged by size:
1548 466 244 76 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 500 1163 2430
Size: 500 663 1267
Fragments arranged by size:
1267 663 500
MscI TGG'CCA
Cuts at: 0 943 2430
Size: 943 1487
MslI CAynn'nnrTG
Cuts at: 0 106 811 1006 1020 1690 2430
Size: 106 705 195 14 670 740
Fragments arranged by size:
740 705 670 195 106 14
MspA1I CmG'CkG
Cuts at: 0 639 926 1212 1254 1438 1527 1799 2345
Size: 639 287 286 42 184 89 272 546
Cuts at: 2345 2430
Size: 85
Fragments arranged by size:
639 546 287 286 272 184 89 85
42
NaeI GCC'GGC
Cuts at: 0 554 2430
Size: 554 1876
NcoI C'CATG_G
Cuts at: 0 578 2430
Size: 578 1852
NgoMIV G'CCGG_C
Cuts at: 0 552 2430
Size: 552 1878
NspI r_CATG'y
Cuts at: 0 795 1011 2430
Size: 795 216 1419
Fragments arranged by size:
1419 795 216
PflMI CCAn_nnn'nTGG
Cuts at: 0 939 2430
Size: 939 1491
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2353 2385 2430
Size: 2353 32 45
Fragments arranged by size:
2353 45 32
PpuMI rG'GwC_Cy
Cuts at: 0 1347 2364 2430
Size: 1347 1017 66
Fragments arranged by size:
1347 1017 66
PspOMI G'GGCC_C
Cuts at: 0 276 512 2201 2430
Size: 276 236 1689 229
Fragments arranged by size:
1689 276 236 229
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1582 1614 2430
Size: 1582 32 816
Fragments arranged by size:
1582 816 32
PstI C_TGCA'G
Cuts at: 0 137 418 638 1709 1850 2123 2231 2430
Size: 137 281 220 1071 141 273 108 199
Fragments arranged by size:
1071 281 273 220 199 141 137 108
PvuII CAG'CTG
Cuts at: 0 1212 2430
Size: 1212 1218
SacI G_AGCT'C
Cuts at: 0 20 1628 2180 2430
Size: 20 1608 552 250
Fragments arranged by size:
1608 552 250 20
SapI GCTCTTCn'nnn_
Cuts at: 0 2047 2185 2430
Size: 2047 138 245
Fragments arranged by size:
2047 245 138
SbfI CC_TGCA'GG
Cuts at: 0 1850 2430
Size: 1850 580
ScaI AGT'ACT
Cuts at: 0 370 1051 2430
Size: 370 681 1379
Fragments arranged by size:
1379 681 370
SexAI A'CCwGG_T
Cuts at: 0 380 1331 2430
Size: 380 951 1099
Fragments arranged by size:
1099 951 380
SfcI C'TryA_G
Cuts at: 0 133 414 634 1083 1536 1705 1846 2119
Size: 133 281 220 449 453 169 141 273
Cuts at: 2119 2227 2430
Size: 108 203
Fragments arranged by size:
453 449 281 273 220 203 169 141
133 108
SmlI C'TyrA_G
Cuts at: 0 1042 1351 1411 1636 1651 1759 1861 1876
Size: 1042 309 60 225 15 108 102 15
Cuts at: 1876 1930 2335 2430
Size: 54 405 95
Fragments arranged by size:
1042 405 309 225 108 102 95 60
54 15 15
StyI C'CwwG_G
Cuts at: 0 182 578 719 2430
Size: 182 396 141 1711
Fragments arranged by size:
1711 396 182 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 157 2050 2430
Size: 157 1893 380
Fragments arranged by size:
1893 380 157
TatI w'GTAC_w
Cuts at: 0 314 368 1049 2430
Size: 314 54 681 1381
Fragments arranged by size:
1381 681 314 54
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 319 351 2389 2421 2430
Size: 319 32 2038 32 9
Fragments arranged by size:
2038 319 32 32 9
Tth111I GACn'n_nGTC
Cuts at: 0 645 2430
Size: 645 1785
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 453 2430
Size: 453 1977
XmnI GAAnn'nnTTC
Cuts at: 0 487 745 1111 2430
Size: 487 258 366 1319
Fragments arranged by size:
1319 487 366 258
ZraI GAC'GTC
Cuts at: 0 1101 2430
Size: 1101 1329
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflII AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BamHI
BanI BanII BbsI BbvCI BcgI BclI BglI Bme1580I
BmgBI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstYI
BstZ17I BtgI BtgZI BtsI DraIII EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV NspI PflMI PpiI
PpuMI PspOMI PsrI PstI PvuII SacI SapI SbfI
ScaI SexAI SfcI SmlI StyI TaqII TatI TstI
Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflIII AgeI AscI AseI AsiSI AvrII
BaeI BbeI BciVI BfrBI BglII BlpI BmrI BmtI
BplI BsaI BsaBI BsiWI BsmI BspHI BsrGI BssHII
BstBI BstXI Bsu36I ClaI CspCI DraI DrdI EagI
EciI EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI NarI NdeI NheI
NotI NruI NsiI PacI PasI PciI PfoI PmeI
PmlI PshAI PsiI PspXI PvuI RsrII SacII SalI
SanDI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI XbaI XhoI XmaI