Restriction map for
pF1KSDA1439
(Linear) (Six-base) MAPSORT of: KIAA1439.seq Check: 2914 from: 1 to: 1527
KIAA1439.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
Acc65I G'GTAC_C
Cuts at: 0 1517 1527
Size: 1517 10
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 988 1527
Size: 988 539
AlwNI CAG_nnn'CTG
Cuts at: 0 718 1527
Size: 718 809
ApaLI G'TGCA_C
Cuts at: 0 1279 1527
Size: 1279 248
ApoI r'AATT_y
Cuts at: 0 31 726 1192 1527
Size: 31 695 466 335
Fragments arranged by size:
695 466 335 31
BamHI G'GATC_C
Cuts at: 0 1476 1527
Size: 1476 51
BanI G'GyrC_C
Cuts at: 0 1356 1517 1527
Size: 1356 161 10
Fragments arranged by size:
1356 161 10
BanII G_rGCy'C
Cuts at: 0 482 820 1527
Size: 482 338 707
Fragments arranged by size:
707 482 338
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1076 1110 1527
Size: 1076 34 417
Fragments arranged by size:
1076 417 34
Bme1580I G_kGCm'C
Cuts at: 0 1283 1527
Size: 1283 244
BmgBI CAC'GTC
Cuts at: 0 66 1382 1412 1527
Size: 66 1316 30 115
Fragments arranged by size:
1316 115 66 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 399 1527
Size: 399 1128
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 817 1527
Size: 817 710
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1356 1386 1527
Size: 1356 30 141
Fragments arranged by size:
1356 141 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 801 883 1008 1527
Size: 801 82 125 519
Fragments arranged by size:
801 519 125 82
BseYI C'CCAG_C
Cuts at: 0 580 799 1155 1432 1527
Size: 580 219 356 277 95
Fragments arranged by size:
580 356 277 219 95
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1418 1527
Size: 1418 109
BsiHKAI G_wGCw'C
Cuts at: 0 820 1283 1527
Size: 820 463 244
Fragments arranged by size:
820 463 244
BsmI GAATG_Cn'
Cuts at: 0 953 1527
Size: 953 574
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1174 1527
Size: 1174 353
Bsp1286I G_dGCh'C
Cuts at: 0 482 820 1283 1527
Size: 482 338 463 244
Fragments arranged by size:
482 463 338 244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 104 1527
Size: 104 1423
BsrBI CCG'CTC
Cuts at: 0 12 1527
Size: 12 1515
BsrDI GCAATG_nn'
Cuts at: 0 788 1527
Size: 788 739
BsrFI r'CCGG_y
Cuts at: 0 1353 1527
Size: 1353 174
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1135 1527
Size: 1135 392
BstYI r'GATC_y
Cuts at: 0 1476 1527
Size: 1476 51
Bsu36I CC'TnA_GG
Cuts at: 0 1175 1527
Size: 1175 352
BtgI C'CryG_G
Cuts at: 0 1471 1527
Size: 1471 56
DraI TTT'AAA
Cuts at: 0 411 1527
Size: 411 1116
DrdI GACnn_nn'nnGTC
Cuts at: 0 649 1527
Size: 649 878
EaeI y'GGCC_r
Cuts at: 0 1334 1527
Size: 1334 193
EarI CTCTTCn'nnn_
Cuts at: 0 149 1106 1527
Size: 149 957 421
Fragments arranged by size:
957 421 149
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 349 1527
Size: 349 1178
EcoICRI GAG'CTC
Cuts at: 0 818 1527
Size: 818 709
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 399 988 1527
Size: 399 589 539
Fragments arranged by size:
589 539 399
EcoNI CCTnn'n_nnAGG
Cuts at: 0 361 1527
Size: 361 1166
EcoO109I rG'GnC_Cy
Cuts at: 0 1066 1527
Size: 1066 461
EcoRV GAT'ATC
Cuts at: 0 246 1527
Size: 246 1281
HaeII r_GCGC'y
Cuts at: 0 447 831 897 1527
Size: 447 384 66 630
Fragments arranged by size:
630 447 384 66
HincII GTy'rAC
Cuts at: 0 1385 1527
Size: 1385 142
HindIII A'AGCT_T
Cuts at: 0 1482 1527
Size: 1482 45
KpnI G_GTAC'C
Cuts at: 0 1521 1527
Size: 1521 6
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 273 341 365 513 956 982 1224 1399
Size: 273 68 24 148 443 26 242 175
Cuts at: 1399 1446 1527
Size: 47 81
Fragments arranged by size:
443 273 242 175 148 81 68 47
26 24
MscI TGG'CCA
Cuts at: 0 1336 1527
Size: 1336 191
MslI CAynn'nnrTG
Cuts at: 0 1303 1527
Size: 1303 224
MspA1I CmG'CkG
Cuts at: 0 423 1527
Size: 423 1104
NspI r_CATG'y
Cuts at: 0 553 1087 1527
Size: 553 534 440
Fragments arranged by size:
553 534 440
PasI CC'CwG_GG
Cuts at: 0 1023 1527
Size: 1023 504
PflMI CCAn_nnn'nTGG
Cuts at: 0 622 907 1527
Size: 622 285 620
Fragments arranged by size:
622 620 285
PfoI T'CCnGG_A
Cuts at: 0 638 774 1527
Size: 638 136 753
Fragments arranged by size:
753 638 136
PpuMI rG'GwC_Cy
Cuts at: 0 1066 1527
Size: 1066 461
PstI C_TGCA'G
Cuts at: 0 101 714 1527
Size: 101 613 813
Fragments arranged by size:
813 613 101
SacI G_AGCT'C
Cuts at: 0 820 1527
Size: 820 707
SbfI CC_TGCA'GG
Cuts at: 0 101 1527
Size: 101 1426
ScaI AGT'ACT
Cuts at: 0 432 1527
Size: 432 1095
SfcI C'TryA_G
Cuts at: 0 97 710 762 1527
Size: 97 613 52 765
Fragments arranged by size:
765 613 97 52
SmlI C'TyrA_G
Cuts at: 0 832 1527
Size: 832 695
SphI G_CATG'C
Cuts at: 0 553 1087 1527
Size: 553 534 440
Fragments arranged by size:
553 534 440
StuI AGG'CCT
Cuts at: 0 1341 1527
Size: 1341 186
StyI C'CwwG_G
Cuts at: 0 888 1269 1527
Size: 888 381 258
Fragments arranged by size:
888 381 258
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 37 1134 1169 1446 1527
Size: 37 1097 35 277 81
Fragments arranged by size:
1097 277 81 37 35
TatI w'GTAC_w
Cuts at: 0 430 1527
Size: 430 1097
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1109 1141 1356 1388 1527
Size: 1109 32 215 32 139
Fragments arranged by size:
1109 215 139 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1509 1527
Size: 1509 18
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 619 1527
Size: 619 908
XmnI GAAnn'nnTTC
Cuts at: 0 53 1527
Size: 53 1474
Enzymes that do cut:
Acc65I AcuI AlwNI ApaLI ApoI BamHI BanI BanII
BcgI Bme1580I BmgBI BpmI BpuEI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BstXI BstYI Bsu36I BtgI DraI DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV HaeII
HincII HindIII KpnI MmeI MscI MslI MspA1I NspI
PasI PflMI PfoI PpuMI PstI SacI SbfI ScaI
SfcI SmlI SphI StuI StyI TaqII TatI TstI
Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI BmrI BmtI BplI Bpu10I
BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BspEI
BspHI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I
BtgZI BtsI ClaI CspCI DraIII EagI EcoRI FalI
FseI FspI FspAI Hin4I HpaI KasI MfeI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI
SanDI SapI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI SwaI XbaI XhoI XmaI
ZraI