Restriction map for
pF1KSDA1442
(Linear) (Six-base) MAPSORT of: KIAA1442.seq Check: 955 from: 1 to: 1620
KIAA1442.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AccI GT'mk_AC
Cuts at: 0 998 1620
Size: 998 622
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 607 1126 1192 1269 1620
Size: 52 555 519 66 77 351
Fragments arranged by size:
555 519 351 77 66 52
AflIII A'CryG_T
Cuts at: 0 660 683 1620
Size: 660 23 937
Fragments arranged by size:
937 660 23
AhdI GACnn_n'nnGTC
Cuts at: 0 390 1620
Size: 390 1230
AleI CACnn'nnGTG
Cuts at: 0 643 1620
Size: 643 977
AlwNI CAG_nnn'CTG
Cuts at: 0 1164 1620
Size: 1164 456
ApaI G_GGCC'C
Cuts at: 0 1331 1620
Size: 1331 289
ApaLI G'TGCA_C
Cuts at: 0 691 1620
Size: 691 929
AscI GG'CGCG_CC
Cuts at: 0 721 1448 1620
Size: 721 727 172
Fragments arranged by size:
727 721 172
AvaI C'yCGr_G
Cuts at: 0 286 775 928 1078 1222 1245 1323 1330
Size: 286 489 153 150 144 23 78 7
Cuts at: 1330 1620
Size: 290
Fragments arranged by size:
489 290 286 153 150 144 78 23
7
BamHI G'GATC_C
Cuts at: 0 311 1620
Size: 311 1309
BanI G'GyrC_C
Cuts at: 0 799 1620
Size: 799 821
BanII G_rGCy'C
Cuts at: 0 67 287 881 1019 1331 1591 1620
Size: 67 220 594 138 312 260 29
Fragments arranged by size:
594 312 260 220 138 67 29
BbeI G_GCGC'C
Cuts at: 0 803 1620
Size: 803 817
BglI GCCn_nnn'nGGC
Cuts at: 0 918 1123 1439 1620
Size: 918 205 316 181
Fragments arranged by size:
918 316 205 181
Bme1580I G_kGCm'C
Cuts at: 0 695 1148 1331 1620
Size: 695 453 183 289
Fragments arranged by size:
695 453 289 183
BmgBI CAC'GTC
Cuts at: 0 1188 1620
Size: 1188 432
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 469 537 1620
Size: 469 68 1083
Fragments arranged by size:
1083 469 68
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 1620
Size: 538 1082
BsaI GGTCTCn'nnnn_
Cuts at: 0 1067 1620
Size: 1067 553
BsaAI yAC'GTr
Cuts at: 0 372 663 1620
Size: 372 291 957
Fragments arranged by size:
957 372 291
BsaHI Gr'CG_yC
Cuts at: 0 104 517 800 910 1316 1620
Size: 104 413 283 110 406 304
Fragments arranged by size:
413 406 304 283 110 104
BsaWI w'CCGG_w
Cuts at: 0 488 506 1620
Size: 488 18 1114
Fragments arranged by size:
1114 488 18
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 685 715 1243 1273 1620
Size: 685 30 528 30 347
Fragments arranged by size:
685 528 347 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 52 1620
Size: 52 1568
BseYI C'CCAG_C
Cuts at: 0 1384 1486 1620
Size: 1384 102 134
Fragments arranged by size:
1384 134 102
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 492 925 1620
Size: 492 433 695
Fragments arranged by size:
695 492 433
BsiEI CG_ry'CG
Cuts at: 0 219 990 1118 1620
Size: 219 771 128 502
Fragments arranged by size:
771 502 219 128
BsiHKAI G_wGCw'C
Cuts at: 0 147 695 866 881 1620
Size: 147 548 171 15 739
Fragments arranged by size:
739 548 171 147 15
BsmI GAATG_Cn'
Cuts at: 0 597 1620
Size: 597 1023
BsmBI CGTCTCn'nnnn_
Cuts at: 0 382 508 1620
Size: 382 126 1112
Fragments arranged by size:
1112 382 126
Bsp1286I G_dGCh'C
Cuts at: 0 67 147 287 695 866 881 1019 1148
Size: 67 80 140 408 171 15 138 129
Cuts at: 1148 1331 1591 1620
Size: 183 260 29
Fragments arranged by size:
408 260 183 171 140 138 129 80
67 29 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 834 1620
Size: 834 786
BsrBI CCG'CTC
Cuts at: 0 1230 1406 1620
Size: 1230 176 214
Fragments arranged by size:
1230 214 176
BsrFI r'CCGG_y
Cuts at: 0 55 218 476 1462 1620
Size: 55 163 258 986 158
Fragments arranged by size:
986 258 163 158 55
BsrGI T'GTAC_A
Cuts at: 0 1202 1620
Size: 1202 418
BssHII G'CGCG_C
Cuts at: 0 721 1176 1345 1448 1620
Size: 721 455 169 103 172
Fragments arranged by size:
721 455 172 169 103
BssSI C'ACGA_G
Cuts at: 0 140 865 1620
Size: 140 725 755
Fragments arranged by size:
755 725 140
BstAPI GCAn_nnn'nTGC
Cuts at: 0 479 980 1620
Size: 479 501 640
Fragments arranged by size:
640 501 479
BstEII G'GTnAC_C
Cuts at: 0 942 1620
Size: 942 678
BstYI r'GATC_y
Cuts at: 0 311 1620
Size: 311 1309
Bsu36I CC'TnA_GG
Cuts at: 0 175 1620
Size: 175 1445
BtgI C'CryG_G
Cuts at: 0 113 233 791 916 1371 1591 1620
Size: 113 120 558 125 455 220 29
Fragments arranged by size:
558 455 220 125 120 113 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1287 1620
Size: 1287 333
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 792 827 1620
Size: 792 35 793
Fragments arranged by size:
793 792 35
DraIII CAC_nnn'GTG
Cuts at: 0 201 1620
Size: 201 1419
EaeI y'GGCC_r
Cuts at: 0 206 668 711 987 1115 1190 1298 1460
Size: 206 462 43 276 128 75 108 162
Cuts at: 1460 1620
Size: 160
Fragments arranged by size:
462 276 206 162 160 128 108 75
43
EagI C'GGCC_G
Cuts at: 0 987 1115 1620
Size: 987 128 505
Fragments arranged by size:
987 505 128
EarI CTCTTCn'nnn_
Cuts at: 0 487 1620
Size: 487 1133
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 307 1198 1251 1620
Size: 307 891 53 369
Fragments arranged by size:
891 369 307 53
EcoICRI GAG'CTC
Cuts at: 0 879 1620
Size: 879 741
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 607 1126 1192 1269 1620
Size: 52 555 519 66 77 351
Fragments arranged by size:
555 519 351 77 66 52
EcoNI CCTnn'n_nnAGG
Cuts at: 0 34 1620
Size: 34 1586
EcoO109I rG'GnC_Cy
Cuts at: 0 1327 1481 1620
Size: 1327 154 139
Fragments arranged by size:
1327 154 139
FseI GG_CCGG'CC
Cuts at: 0 1466 1620
Size: 1466 154
HaeII r_GCGC'y
Cuts at: 0 803 1536 1620
Size: 803 733 84
Fragments arranged by size:
803 733 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 376 408 1620
Size: 376 32 1212
Fragments arranged by size:
1212 376 32
KasI G'GCGC_C
Cuts at: 0 799 1620
Size: 799 821
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 209 701 1620
Size: 194 15 492 919
Fragments arranged by size:
919 492 194 15
MscI TGG'CCA
Cuts at: 0 208 1300 1620
Size: 208 1092 320
Fragments arranged by size:
1092 320 208
MslI CAynn'nnrTG
Cuts at: 0 643 688 901 1620
Size: 643 45 213 719
Fragments arranged by size:
719 643 213 45
MspA1I CmG'CkG
Cuts at: 0 20 45 115 918 1296 1352 1373 1620
Size: 20 25 70 803 378 56 21 247
Fragments arranged by size:
803 378 247 70 56 25 21 20
NaeI GCC'GGC
Cuts at: 0 57 1464 1620
Size: 57 1407 156
Fragments arranged by size:
1407 156 57
NarI GG'CG_CC
Cuts at: 0 800 1620
Size: 800 820
NgoMIV G'CCGG_C
Cuts at: 0 55 1462 1620
Size: 55 1407 158
Fragments arranged by size:
1407 158 55
NspI r_CATG'y
Cuts at: 0 615 687 1620
Size: 615 72 933
Fragments arranged by size:
933 615 72
PciI A'CATG_T
Cuts at: 0 683 1620
Size: 683 937
PflMI CCAn_nnn'nTGG
Cuts at: 0 630 1620
Size: 630 990
PfoI T'CCnGG_A
Cuts at: 0 76 98 1620
Size: 76 22 1522
Fragments arranged by size:
1522 76 22
PmlI CAC'GTG
Cuts at: 0 663 1620
Size: 663 957
PpuMI rG'GwC_Cy
Cuts at: 0 1481 1620
Size: 1481 139
PspOMI G'GGCC_C
Cuts at: 0 1327 1620
Size: 1327 293
PstI C_TGCA'G
Cuts at: 0 1357 1620
Size: 1357 263
PvuII CAG'CTG
Cuts at: 0 1352 1620
Size: 1352 268
SacI G_AGCT'C
Cuts at: 0 881 1620
Size: 881 739
SacII CC_GC'GG
Cuts at: 0 116 919 1374 1620
Size: 116 803 455 246
Fragments arranged by size:
803 455 246 116
SanDI GG'GwC_CC
Cuts at: 0 1481 1620
Size: 1481 139
SapI GCTCTTCn'nnn_
Cuts at: 0 487 1620
Size: 487 1133
SfcI C'TryA_G
Cuts at: 0 1045 1353 1620
Size: 1045 308 267
Fragments arranged by size:
1045 308 267
SfoI GGC'GCC
Cuts at: 0 801 1620
Size: 801 819
SmaI CCC'GGG
Cuts at: 0 288 777 930 1224 1325 1620
Size: 288 489 153 294 101 295
Fragments arranged by size:
489 295 294 288 153 101
SmlI C'TyrA_G
Cuts at: 0 553 1620
Size: 553 1067
StuI AGG'CCT
Cuts at: 0 1577 1620
Size: 1577 43
StyI C'CwwG_G
Cuts at: 0 1091 1526 1620
Size: 1091 435 94
Fragments arranged by size:
1091 435 94
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 293 472 1268 1620
Size: 293 179 796 352
Fragments arranged by size:
796 352 293 179
TatI w'GTAC_w
Cuts at: 0 1202 1620
Size: 1202 418
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 483 515 685 717 1243 1275 1620
Size: 483 32 170 32 526 32 345
Fragments arranged by size:
526 483 345 170 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 529 1620
Size: 529 1091
XmaI C'CCGG_G
Cuts at: 0 286 775 928 1222 1323 1620
Size: 286 489 153 294 101 297
Fragments arranged by size:
489 297 294 286 153 101
Enzymes that do cut:
AccI AcuI AflIII AhdI AleI AlwNI ApaI ApaLI
AscI AvaI BamHI BanI BanII BbeI BglI Bme1580I
BmgBI BplI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII
BstYI Bsu36I BtgI BtgZI CspCI DraIII EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FseI HaeII
Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PflMI PfoI PmlI PpuMI PspOMI
PstI PvuII SacI SacII SanDI SapI SfcI SfoI
SmaI SmlI StuI StyI TaqII TatI TstI Tth111I
XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AlfI
AloI ApoI AseI AsiSI AvrII BaeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI BmrI BmtI
BpmI Bpu10I BsaBI BsiWI BspEI BspHI BsrDI BstBI
BstXI BstZ17I BtsI ClaI DraI DrdI EcoRI EcoRV
FalI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SalI SbfI ScaI SexAI SfiI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI XbaI XcmI
XhoI XmnI ZraI