Restriction map for
pF1KA1448
(Linear) (Six-base) MAPSORT of: KIAA1448.seq Check: 5642 from: 1 to: 3459
KIAA1448.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AatII G_ACGT'C
Cuts at: 0 1228 2375 2417 2754 3459
Size: 1228 1147 42 337 705
Fragments arranged by size:
1228 1147 705 337 42
AccI GT'mk_AC
Cuts at: 0 1258 3459
Size: 1258 2201
AclI AA'CG_TT
Cuts at: 0 1287 3459
Size: 1287 2172
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 132 1138 2238 3459
Size: 132 1006 1100 1221
Fragments arranged by size:
1221 1100 1006 132
AfeI AGC'GCT
Cuts at: 0 1666 3459
Size: 1666 1793
AflII C'TTAA_G
Cuts at: 0 2538 3459
Size: 2538 921
AflIII A'CryG_T
Cuts at: 0 2706 3459
Size: 2706 753
AhdI GACnn_n'nnGTC
Cuts at: 0 2013 3459
Size: 2013 1446
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 256 314 3459
Size: 256 58 3145
Fragments arranged by size:
3145 256 58
AlwNI CAG_nnn'CTG
Cuts at: 0 1143 1549 1974 3377 3459
Size: 1143 406 425 1403 82
Fragments arranged by size:
1403 1143 425 406 82
ApoI r'AATT_y
Cuts at: 0 443 933 2207 2777 3459
Size: 443 490 1274 570 682
Fragments arranged by size:
1274 682 570 490 443
AseI AT'TA_AT
Cuts at: 0 812 3113 3459
Size: 812 2301 346
Fragments arranged by size:
2301 812 346
AvaI C'yCGr_G
Cuts at: 0 50 3459
Size: 50 3409
AvrII C'CTAG_G
Cuts at: 0 1428 3459
Size: 1428 2031
BamHI G'GATC_C
Cuts at: 0 2730 3042 3459
Size: 2730 312 417
Fragments arranged by size:
2730 417 312
BanI G'GyrC_C
Cuts at: 0 775 1556 3459
Size: 775 781 1903
Fragments arranged by size:
1903 781 775
BanII G_rGCy'C
Cuts at: 0 979 1584 1658 3064 3459
Size: 979 605 74 1406 395
Fragments arranged by size:
1406 979 605 395 74
BbeI G_GCGC'C
Cuts at: 0 1560 3459
Size: 1560 1899
BbsI GAAGACnn'nnnn_
Cuts at: 0 1484 1804 2417 3459
Size: 1484 320 613 1042
Fragments arranged by size:
1484 1042 613 320
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2806 2840 3459
Size: 2806 34 619
Fragments arranged by size:
2806 619 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2373 3459
Size: 2373 1086
BfrBI ATG'CAT
Cuts at: 0 77 3459
Size: 77 3382
BglI GCCn_nnn'nGGC
Cuts at: 0 347 3459
Size: 347 3112
BglII A'GATC_T
Cuts at: 0 1919 1950 2199 3459
Size: 1919 31 249 1260
Fragments arranged by size:
1919 1260 249 31
BlpI GC'TnA_GC
Cuts at: 0 1550 1573 1787 3459
Size: 1550 23 214 1672
Fragments arranged by size:
1672 1550 214 23
BmgBI CAC'GTC
Cuts at: 0 2746 3459
Size: 2746 713
BmrI ACTGGGnnnn_n'
Cuts at: 0 2435 3042 3459
Size: 2435 607 417
Fragments arranged by size:
2435 607 417
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1598 1836 2364 3459
Size: 1598 238 528 1095
Fragments arranged by size:
1598 1095 528 238
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1286 2373 3335 3459
Size: 1286 1087 962 124
Fragments arranged by size:
1286 1087 962 124
Bpu10I CC'TnA_GC
Cuts at: 0 1136 1719 2962 3459
Size: 1136 583 1243 497
Fragments arranged by size:
1243 1136 583 497
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2187 2440 3050 3459
Size: 2187 253 610 409
Fragments arranged by size:
2187 610 409 253
BsaI GGTCTCn'nnnn_
Cuts at: 0 49 176 691 1807 3459
Size: 49 127 515 1116 1652
Fragments arranged by size:
1652 1116 515 127 49
BsaAI yAC'GTr
Cuts at: 0 1680 3459
Size: 1680 1779
BsaHI Gr'CG_yC
Cuts at: 0 1225 1557 2372 2414 2751 3459
Size: 1225 332 815 42 337 708
Fragments arranged by size:
1225 815 708 337 332 42
BsaWI w'CCGG_w
Cuts at: 0 1610 2864 3459
Size: 1610 1254 595
Fragments arranged by size:
1610 1254 595
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 29 526 556 3459
Size: 29 497 30 2903
Fragments arranged by size:
2903 497 30 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1117 1283 2539 3423 3459
Size: 1117 166 1256 884 36
Fragments arranged by size:
1256 1117 884 166 36
BseYI C'CCAG_C
Cuts at: 0 592 1700 2068 2714 3370 3459
Size: 592 1108 368 646 656 89
Fragments arranged by size:
1108 656 646 592 368 89
BsiHKAI G_wGCw'C
Cuts at: 0 1012 1137 3459
Size: 1012 125 2322
Fragments arranged by size:
2322 1012 125
Bsp1286I G_dGCh'C
Cuts at: 0 979 1012 1137 1584 1658 3064 3459
Size: 979 33 125 447 74 1406 395
Fragments arranged by size:
1406 979 447 395 125 74 33
BspEI T'CCGG_A
Cuts at: 0 1610 3459
Size: 1610 1849
BsrBI CCG'CTC
Cuts at: 0 1554 1577 2343 3459
Size: 1554 23 766 1116
Fragments arranged by size:
1554 1116 766 23
BsrDI GCAATG_nn'
Cuts at: 0 572 965 1056 1310 3339 3459
Size: 572 393 91 254 2029 120
Fragments arranged by size:
2029 572 393 254 120 91
BsrGI T'GTAC_A
Cuts at: 0 218 3249 3459
Size: 218 3031 210
Fragments arranged by size:
3031 218 210
BssSI C'ACGA_G
Cuts at: 0 1781 3459
Size: 1781 1678
BstAPI GCAn_nnn'nTGC
Cuts at: 0 964 1030 3459
Size: 964 66 2429
Fragments arranged by size:
2429 964 66
BstEII G'GTnAC_C
Cuts at: 0 3232 3459
Size: 3232 227
BstXI CCAn_nnnn'nTGG
Cuts at: 0 612 1368 3459
Size: 612 756 2091
Fragments arranged by size:
2091 756 612
BstYI r'GATC_y
Cuts at: 0 189 537 741 1919 1950 2199 2584 2730
Size: 189 348 204 1178 31 249 385 146
Cuts at: 2730 3042 3459
Size: 312 417
Fragments arranged by size:
1178 417 385 348 312 249 204 189
146 31
BtgI C'CryG_G
Cuts at: 0 980 1166 3055 3206 3400 3459
Size: 980 186 1889 151 194 59
Fragments arranged by size:
1889 980 194 186 151 59
BtsI GCAGTG_nn'
Cuts at: 0 2118 2366 3239 3459
Size: 2118 248 873 220
Fragments arranged by size:
2118 873 248 220
DraI TTT'AAA
Cuts at: 0 1292 3459
Size: 1292 2167
DraIII CAC_nnn'GTG
Cuts at: 0 657 3459
Size: 657 2802
DrdI GACnn_nn'nnGTC
Cuts at: 0 2750 3459
Size: 2750 709
EaeI y'GGCC_r
Cuts at: 0 854 1536 2285 2363 2420 3168 3459
Size: 854 682 749 78 57 748 291
Fragments arranged by size:
854 749 748 682 291 78 57
EarI CTCTTCn'nnn_
Cuts at: 0 1331 1589 2018 2999 3459
Size: 1331 258 429 981 460
Fragments arranged by size:
1331 981 460 429 258
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 132 1138 1286 2238 2373 3335 3459
Size: 132 1006 148 952 135 962 124
Fragments arranged by size:
1006 962 952 148 135 132 124
EcoO109I rG'GnC_Cy
Cuts at: 0 38 1068 1122 1140 1172 3459
Size: 38 1030 54 18 32 2287
Fragments arranged by size:
2287 1030 54 38 32 18
EcoRV GAT'ATC
Cuts at: 0 987 3459
Size: 987 2472
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1360 1969 2892 3459
Size: 1360 609 923 567
Fragments arranged by size:
1360 923 609 567
HaeII r_GCGC'y
Cuts at: 0 1560 1668 2166 3273 3459
Size: 1560 108 498 1107 186
Fragments arranged by size:
1560 1107 498 186 108
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 254 286 526 558 1443 1475 1999 2031
Size: 254 32 240 32 885 32 524 32
Cuts at: 2031 2063 2571 2603 3459
Size: 32 508 32 856
Fragments arranged by size:
885 856 524 508 254 240 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 0 1467 3459
Size: 1467 1992
HindIII A'AGCT_T
Cuts at: 0 1342 3459
Size: 1342 2117
HpaI GTT'AAC
Cuts at: 0 1467 3459
Size: 1467 1992
KasI G'GCGC_C
Cuts at: 0 1556 3459
Size: 1556 1903
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3124 3459
Size: 3124 335
MscI TGG'CCA
Cuts at: 0 1538 2287 2365 2422 3170 3459
Size: 1538 749 78 57 748 289
Fragments arranged by size:
1538 749 748 289 78 57
MslI CAynn'nnrTG
Cuts at: 0 85 363 692 1366 3459
Size: 85 278 329 674 2093
Fragments arranged by size:
2093 674 329 278 85
MspA1I CmG'CkG
Cuts at: 0 982 1631 1713 2718 3208 3402 3459
Size: 982 649 82 1005 490 194 57
Fragments arranged by size:
1005 982 649 490 194 82 57
NarI GG'CG_CC
Cuts at: 0 1557 3459
Size: 1557 1902
NcoI C'CATG_G
Cuts at: 0 1166 3055 3459
Size: 1166 1889 404
Fragments arranged by size:
1889 1166 404
NsiI A_TGCA'T
Cuts at: 0 79 3459
Size: 79 3380
PflMI CCAn_nnn'nTGG
Cuts at: 0 520 2113 3459
Size: 520 1593 1346
Fragments arranged by size:
1593 1346 520
PfoI T'CCnGG_A
Cuts at: 0 2350 3459
Size: 2350 1109
PpuMI rG'GwC_Cy
Cuts at: 0 1068 1122 1172 3459
Size: 1068 54 50 2287
Fragments arranged by size:
2287 1068 54 50
PsiI TTA'TAA
Cuts at: 0 3308 3459
Size: 3308 151
PvuII CAG'CTG
Cuts at: 0 1713 2718 3459
Size: 1713 1005 741
Fragments arranged by size:
1713 1005 741
SacII CC_GC'GG
Cuts at: 0 983 3209 3403 3459
Size: 983 2226 194 56
Fragments arranged by size:
2226 983 194 56
SanDI GG'GwC_CC
Cuts at: 0 1172 3459
Size: 1172 2287
SapI GCTCTTCn'nnn_
Cuts at: 0 1589 3459
Size: 1589 1870
SfcI C'TryA_G
Cuts at: 0 422 897 1906 3378 3459
Size: 422 475 1009 1472 81
Fragments arranged by size:
1472 1009 475 422 81
SfoI GGC'GCC
Cuts at: 0 1558 3459
Size: 1558 1901
SmlI C'TyrA_G
Cuts at: 0 50 2166 2455 2538 3065 3459
Size: 50 2116 289 83 527 394
Fragments arranged by size:
2116 527 394 289 83 50
SnaBI TAC'GTA
Cuts at: 0 1680 3459
Size: 1680 1779
SspI AAT'ATT
Cuts at: 0 810 3459
Size: 810 2649
StyI C'CwwG_G
Cuts at: 0 851 1166 1428 3055 3459
Size: 851 315 262 1627 404
Fragments arranged by size:
1627 851 404 315 262
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 527 691 3441 3459
Size: 527 164 2750 18
Fragments arranged by size:
2750 527 164 18
TatI w'GTAC_w
Cuts at: 0 218 909 3249 3459
Size: 218 691 2340 210
Fragments arranged by size:
2340 691 218 210
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 31 515 547 2109 2141 3459
Size: 31 484 32 1562 32 1318
Fragments arranged by size:
1562 1318 484 32 32 31
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1080 2722 3459
Size: 1080 1642 737
Fragments arranged by size:
1642 1080 737
XhoI C'TCGA_G
Cuts at: 0 50 3459
Size: 50 3409
XmnI GAAnn'nnTTC
Cuts at: 0 3153 3459
Size: 3153 306
ZraI GAC'GTC
Cuts at: 0 1226 2373 2415 2752 3459
Size: 1226 1147 42 337 707
Fragments arranged by size:
1226 1147 707 337 42
Enzymes that do cut:
AatII AccI AclI AcuI AfeI AflII AflIII AhdI
AlfI AlwNI ApoI AseI AvaI AvrII BamHI BanI
BanII BbeI BbsI BcgI BciVI BfrBI BglI BglII
BlpI BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI Bsp1286I
BspEI BsrBI BsrDI BsrGI BssSI BstAPI BstEII BstXI
BstYI BtgI BtsI DraI DraIII DrdI EaeI EarI
Eco57MI EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII
HpaI KasI MmeI MscI MslI MspA1I NarI NcoI
NsiI PflMI PfoI PpuMI PsiI PvuII SacII SanDI
SapI SfcI SfoI SmlI SnaBI SspI StyI TaqII
TatI TstI XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AgeI AleI AloI ApaI ApaLI AscI
AsiSI BaeI BbvCI BclI Bme1580I BmtI BsaBI BsgI
BsiEI BsiWI BsmI BsmBI BspHI BspMI BsrFI BssHII
BstBI BstZ17I Bsu36I BtgZI ClaI CspCI EagI EciI
EcoICRI EcoNI EcoRI FseI FspI FspAI KpnI MfeI
MluI NaeI NdeI NgoMIV NheI NotI NruI NspI
PacI PasI PciI PmeI PmlI PpiI PshAI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SalI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SpeI SphI
SrfI StuI SwaI Tth111I XbaI XmaI