Restriction map for
pF1KA1449
(Linear) (Six-base) MAPSORT of: KIAA1449.seq Check: 4448 from: 1 to: 2031
KIAA1449.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AccI GT'mk_AC
Cuts at: 0 955 1265 2031
Size: 955 310 766
Fragments arranged by size:
955 766 310
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1706 2031
Size: 1706 325
AflIII A'CryG_T
Cuts at: 0 595 795 1431 1794 2031
Size: 595 200 636 363 237
Fragments arranged by size:
636 595 363 237 200
AleI CACnn'nnGTG
Cuts at: 0 796 2031
Size: 796 1235
ApoI r'AATT_y
Cuts at: 0 983 1380 1681 1691 2031
Size: 983 397 301 10 340
Fragments arranged by size:
983 397 340 301 10
AseI AT'TA_AT
Cuts at: 0 268 2031
Size: 268 1763
BamHI G'GATC_C
Cuts at: 0 114 585 1944 2031
Size: 114 471 1359 87
Fragments arranged by size:
1359 471 114 87
BfrBI ATG'CAT
Cuts at: 0 1717 2031
Size: 1717 314
BglI GCCn_nnn'nGGC
Cuts at: 0 680 2031
Size: 680 1351
BglII A'GATC_T
Cuts at: 0 1188 2031
Size: 1188 843
Bme1580I G_kGCm'C
Cuts at: 0 626 1529 2031
Size: 626 903 502
Fragments arranged by size:
903 626 502
BmrI ACTGGGnnnn_n'
Cuts at: 0 544 2031
Size: 544 1487
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1025 1580 2031
Size: 1025 555 451
Fragments arranged by size:
1025 555 451
Bpu10I CC'TnA_GC
Cuts at: 0 366 2031
Size: 366 1665
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 877 2031
Size: 877 1154
BsaI GGTCTCn'nnnn_
Cuts at: 0 1991 2031
Size: 1991 40
BsaAI yAC'GTr
Cuts at: 0 357 2031
Size: 357 1674
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1382 1412 2031
Size: 1382 30 619
Fragments arranged by size:
1382 619 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 914 1921 2031
Size: 914 1007 110
Fragments arranged by size:
1007 914 110
BseYI C'CCAG_C
Cuts at: 0 403 2031
Size: 403 1628
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 1519 2031
Size: 64 1455 512
Fragments arranged by size:
1455 512 64
BsiEI CG_ry'CG
Cuts at: 0 147 2031
Size: 147 1884
BsmI GAATG_Cn'
Cuts at: 0 312 2031
Size: 312 1719
Bsp1286I G_dGCh'C
Cuts at: 0 626 1529 2031
Size: 626 903 502
Fragments arranged by size:
903 626 502
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 36 2031
Size: 36 1995
BsrBI CCG'CTC
Cuts at: 0 1991 2031
Size: 1991 40
BsrDI GCAATG_nn'
Cuts at: 0 643 676 2031
Size: 643 33 1355
Fragments arranged by size:
1355 643 33
BsrFI r'CCGG_y
Cuts at: 0 14 142 2031
Size: 14 128 1889
Fragments arranged by size:
1889 128 14
BsrGI T'GTAC_A
Cuts at: 0 832 1024 1048 2031
Size: 832 192 24 983
Fragments arranged by size:
983 832 192 24
BstAPI GCAn_nnn'nTGC
Cuts at: 0 457 671 1339 2031
Size: 457 214 668 692
Fragments arranged by size:
692 668 457 214
BstBI TT'CG_AA
Cuts at: 0 1962 2031
Size: 1962 69
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1477 2031
Size: 1477 554
BstYI r'GATC_y
Cuts at: 0 114 192 585 1188 1368 1944 2031
Size: 114 78 393 603 180 576 87
Fragments arranged by size:
603 576 393 180 114 87 78
DraI TTT'AAA
Cuts at: 0 1233 2031
Size: 1233 798
EaeI y'GGCC_r
Cuts at: 0 521 717 2031
Size: 521 196 1314
Fragments arranged by size:
1314 521 196
EarI CTCTTCn'nnn_
Cuts at: 0 1445 1851 1981 2031
Size: 1445 406 130 50
Fragments arranged by size:
1445 406 130 50
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 48 2031
Size: 48 1983
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1025 1580 1706 2031
Size: 1025 555 126 325
Fragments arranged by size:
1025 555 325 126
EcoRI G'AATT_C
Cuts at: 0 983 2031
Size: 983 1048
HaeII r_GCGC'y
Cuts at: 0 772 2031
Size: 772 1259
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 140 162 172 194 1382 1414 1597 1629
Size: 140 22 10 22 1188 32 183 32
Cuts at: 1629 1738 1770 1813 1845 2031
Size: 109 32 43 32 186
Fragments arranged by size:
1188 186 183 140 109 43 32 32
32 32 22 22 10
HincII GTy'rAC
Cuts at: 0 332 1286 2031
Size: 332 954 745
Fragments arranged by size:
954 745 332
HindIII A'AGCT_T
Cuts at: 0 613 2031
Size: 613 1418
HpaI GTT'AAC
Cuts at: 0 1286 2031
Size: 1286 745
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 389 1731 2031
Size: 389 1342 300
Fragments arranged by size:
1342 389 300
MscI TGG'CCA
Cuts at: 0 523 719 2031
Size: 523 196 1312
Fragments arranged by size:
1312 523 196
MslI CAynn'nnrTG
Cuts at: 0 796 859 1475 1660 1799 2031
Size: 796 63 616 185 139 232
Fragments arranged by size:
796 616 232 185 139 63
MspA1I CmG'CkG
Cuts at: 0 111 403 916 2031
Size: 111 292 513 1115
Fragments arranged by size:
1115 513 292 111
NdeI CA'TA_TG
Cuts at: 0 372 1981 2031
Size: 372 1609 50
Fragments arranged by size:
1609 372 50
NsiI A_TGCA'T
Cuts at: 0 1719 2031
Size: 1719 312
NspI r_CATG'y
Cuts at: 0 330 599 799 1178 1435 1770 1798 2031
Size: 330 269 200 379 257 335 28 233
Fragments arranged by size:
379 335 330 269 257 233 200 28
PciI A'CATG_T
Cuts at: 0 595 795 1431 1794 2031
Size: 595 200 636 363 237
Fragments arranged by size:
636 595 363 237 200
PflMI CCAn_nnn'nTGG
Cuts at: 0 1941 2031
Size: 1941 90
PfoI T'CCnGG_A
Cuts at: 0 1409 2031
Size: 1409 622
PstI C_TGCA'G
Cuts at: 0 110 2031
Size: 110 1921
PvuII CAG'CTG
Cuts at: 0 111 403 916 2031
Size: 111 292 513 1115
Fragments arranged by size:
1115 513 292 111
SapI GCTCTTCn'nnn_
Cuts at: 0 1851 1981 2031
Size: 1851 130 50
Fragments arranged by size:
1851 130 50
SexAI A'CCwGG_T
Cuts at: 0 1061 1934 2031
Size: 1061 873 97
Fragments arranged by size:
1061 873 97
SfcI C'TryA_G
Cuts at: 0 106 956 2031
Size: 106 850 1075
Fragments arranged by size:
1075 850 106
SmlI C'TyrA_G
Cuts at: 0 892 2031
Size: 892 1139
SnaBI TAC'GTA
Cuts at: 0 357 2031
Size: 357 1674
SpeI A'CTAG_T
Cuts at: 0 390 2031
Size: 390 1641
SspI AAT'ATT
Cuts at: 0 422 1417 2031
Size: 422 995 614
Fragments arranged by size:
995 614 422
StuI AGG'CCT
Cuts at: 0 364 2031
Size: 364 1667
StyI C'CwwG_G
Cuts at: 0 377 714 2031
Size: 377 337 1317
Fragments arranged by size:
1317 377 337
TatI w'GTAC_w
Cuts at: 0 80 243 832 1024 1048 2031
Size: 80 163 589 192 24 983
Fragments arranged by size:
983 589 192 163 80 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1977 2009 2031
Size: 1977 32 22
Fragments arranged by size:
1977 32 22
Tth111I GACn'n_nGTC
Cuts at: 0 1611 2031
Size: 1611 420
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 516 531 1146 2031
Size: 516 15 615 885
Fragments arranged by size:
885 615 516 15
XmnI GAAnn'nnTTC
Cuts at: 0 1975 2031
Size: 1975 56
Enzymes that do cut:
AccI AcuI AflIII AleI ApoI AseI BamHI BfrBI
BglI BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaXI BseRI BseYI BsgI BsiEI BsmI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstBI BstXI
BstYI DraI EaeI EarI EciI Eco57MI EcoRI HaeII
Hin4I HincII HindIII HpaI MmeI MscI MslI MspA1I
NdeI NsiI NspI PciI PflMI PfoI PstI PvuII
SapI SexAI SfcI SmlI SnaBI SpeI SspI StuI
StyI TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BlpI BmgBI BmtI BplI BsaBI BsaHI
BsaWI BsiHKAI BsiWI BsmBI BspEI BspHI BssHII BssSI
BstEII BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraIII DrdI EagI EcoICRI EcoNI EcoO109I EcoRV FalI
FseI FspI FspAI KasI KpnI MfeI MluI NaeI
NarI NcoI NgoMIV NheI NotI NruI PacI PasI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SphI SrfI
SwaI TaqII XbaI XhoI XmaI ZraI