Restriction map for
pF1KSDA1453
(Linear) (Six-base) MAPSORT of: KIAA1453.seq Check: 9618 from: 1 to: 3363
KIAA1453.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 397 2235 2688 3363
Size: 397 1838 453 675
Fragments arranged by size:
1838 675 453 397
AatII G_ACGT'C
Cuts at: 0 812 2314 3363
Size: 812 1502 1049
Fragments arranged by size:
1502 1049 812
Acc65I G'GTAC_C
Cuts at: 0 1181 3363
Size: 1181 2182
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 984 1466 1513 1807 2014 2247 3280 3363
Size: 984 482 47 294 207 233 1033 83
Fragments arranged by size:
1033 984 482 294 233 207 83 47
AfeI AGC'GCT
Cuts at: 0 940 1806 3363
Size: 940 866 1557
Fragments arranged by size:
1557 940 866
AflIII A'CryG_T
Cuts at: 0 263 753 899 3363
Size: 263 490 146 2464
Fragments arranged by size:
2464 490 263 146
AhdI GACnn_n'nnGTC
Cuts at: 0 2373 3363
Size: 2373 990
AleI CACnn'nnGTG
Cuts at: 0 221 3363
Size: 221 3142
AlwNI CAG_nnn'CTG
Cuts at: 0 1656 1768 2466 2972 3078 3363
Size: 1656 112 698 506 106 285
Fragments arranged by size:
1656 698 506 285 112 106
ApaLI G'TGCA_C
Cuts at: 0 1114 3363
Size: 1114 2249
ApoI r'AATT_y
Cuts at: 0 728 1039 1265 3250 3363
Size: 728 311 226 1985 113
Fragments arranged by size:
1985 728 311 226 113
AvaI C'yCGr_G
Cuts at: 0 1119 1288 1335 1530 2959 3363
Size: 1119 169 47 195 1429 404
Fragments arranged by size:
1429 1119 404 195 169 47
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1165 1198 3363
Size: 1165 33 2165
Fragments arranged by size:
2165 1165 33
BanI G'GyrC_C
Cuts at: 0 1181 3363
Size: 1181 2182
BanII G_rGCy'C
Cuts at: 0 1711 1828 2411 2422 2473 2614 3363
Size: 1711 117 583 11 51 141 749
Fragments arranged by size:
1711 749 583 141 117 51 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 342 3173 3363
Size: 342 2831 190
Fragments arranged by size:
2831 342 190
BbvCI CC'TCA_GC
Cuts at: 0 3349 3363
Size: 3349 14
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 825 859 2331 2365 3363
Size: 825 34 1472 34 998
Fragments arranged by size:
1472 998 825 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 677 2083 2463 2512 3363
Size: 677 1406 380 49 851
Fragments arranged by size:
1406 851 677 380 49
BglII A'GATC_T
Cuts at: 0 1687 3363
Size: 1687 1676
BlpI GC'TnA_GC
Cuts at: 0 3019 3363
Size: 3019 344
Bme1580I G_kGCm'C
Cuts at: 0 1118 1145 1463 3363
Size: 1118 27 318 1900
Fragments arranged by size:
1900 1118 318 27
BmgBI CAC'GTC
Cuts at: 0 2480 3363
Size: 2480 883
BmrI ACTGGGnnnn_n'
Cuts at: 0 79 3216 3363
Size: 79 3137 147
Fragments arranged by size:
3137 147 79
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 760 3363
Size: 760 2603
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 838 1287 1812 1821 3363
Size: 178 660 449 525 9 1542
Fragments arranged by size:
1542 660 525 449 178 9
Bpu10I CC'TnA_GC
Cuts at: 0 1649 1748 2008 3349 3363
Size: 1649 99 260 1341 14
Fragments arranged by size:
1649 1341 260 99 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 967 3363
Size: 967 2396
BsaI GGTCTCn'nnnn_
Cuts at: 0 569 3184 3363
Size: 569 2615 179
Fragments arranged by size:
2615 569 179
BsaAI yAC'GTr
Cuts at: 0 266 1158 1248 3363
Size: 266 892 90 2115
Fragments arranged by size:
2115 892 266 90
BsaBI GATnn'nnATC
Cuts at: 0 2831 2912 3363
Size: 2831 81 451
Fragments arranged by size:
2831 451 81
BsaHI Gr'CG_yC
Cuts at: 0 653 809 2311 3363
Size: 653 156 1502 1052
Fragments arranged by size:
1502 1052 653 156
BsaWI w'CCGG_w
Cuts at: 0 1035 3363
Size: 1035 2328
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 313 343 436 466 1736 1766 2028 2058
Size: 313 30 93 30 1270 30 262 30
Cuts at: 2058 2499 2529 3363
Size: 441 30 834
Fragments arranged by size:
1270 834 441 313 262 93 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 78 1305 1308 1610 2036 2322 2760 3282
Size: 78 1227 3 302 426 286 438 522
Cuts at: 3282 3363
Size: 81
Fragments arranged by size:
1227 522 438 426 302 286 81 78
3
BseYI C'CCAG_C
Cuts at: 0 291 2466 3330 3363
Size: 291 2175 864 33
Fragments arranged by size:
2175 864 291 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2770 3363
Size: 2770 593
BsiEI CG_ry'CG
Cuts at: 0 42 1332 2108 3363
Size: 42 1290 776 1255
Fragments arranged by size:
1290 1255 776 42
BsiHKAI G_wGCw'C
Cuts at: 0 263 766 852 1118 1850 3363
Size: 263 503 86 266 732 1513
Fragments arranged by size:
1513 732 503 266 263 86
BsmI GAATG_Cn'
Cuts at: 0 897 1511 3363
Size: 897 614 1852
Fragments arranged by size:
1852 897 614
BsmBI CGTCTCn'nnnn_
Cuts at: 0 567 786 2470 3363
Size: 567 219 1684 893
Fragments arranged by size:
1684 893 567 219
Bsp1286I G_dGCh'C
Cuts at: 0 263 766 852 1118 1145 1463 1711 1828
Size: 263 503 86 266 27 318 248 117
Cuts at: 1828 1850 2411 2422 2473 2614 3363
Size: 22 561 11 51 141 749
Fragments arranged by size:
749 561 503 318 266 263 248 141
117 86 51 27 22 11
BspEI T'CCGG_A
Cuts at: 0 1035 3363
Size: 1035 2328
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 397 455 2235 2688 3363
Size: 397 58 1780 453 675
Fragments arranged by size:
1780 675 453 397 58
BsrBI CCG'CTC
Cuts at: 0 345 1900 3054 3363
Size: 345 1555 1154 309
Fragments arranged by size:
1555 1154 345 309
BsrDI GCAATG_nn'
Cuts at: 0 1176 1380 2118 2278 3363
Size: 1176 204 738 160 1085
Fragments arranged by size:
1176 1085 738 204 160
BsrFI r'CCGG_y
Cuts at: 0 237 2107 3363
Size: 237 1870 1256
Fragments arranged by size:
1870 1256 237
BsrGI T'GTAC_A
Cuts at: 0 2618 3363
Size: 2618 745
BstAPI GCAn_nnn'nTGC
Cuts at: 0 306 389 3340 3363
Size: 306 83 2951 23
Fragments arranged by size:
2951 306 83 23
BstXI CCAn_nnnn'nTGG
Cuts at: 0 223 379 1075 2064 3363
Size: 223 156 696 989 1299
Fragments arranged by size:
1299 989 696 223 156
BstYI r'GATC_y
Cuts at: 0 821 1687 3363
Size: 821 866 1676
Fragments arranged by size:
1676 866 821
Bsu36I CC'TnA_GG
Cuts at: 0 2354 3363
Size: 2354 1009
BtgI C'CryG_G
Cuts at: 0 317 2320 2520 2819 2843 3194 3363
Size: 317 2003 200 299 24 351 169
Fragments arranged by size:
2003 351 317 299 200 169 24
BtsI GCAGTG_nn'
Cuts at: 0 261 1766 2507 3363
Size: 261 1505 741 856
Fragments arranged by size:
1505 856 741 261
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2372 2407 3363
Size: 2372 35 956
Fragments arranged by size:
2372 956 35
DraIII CAC_nnn'GTG
Cuts at: 0 2096 3363
Size: 2096 1267
EaeI y'GGCC_r
Cuts at: 0 39 239 1329 1853 1944 2691 2718 3363
Size: 39 200 1090 524 91 747 27 645
Fragments arranged by size:
1090 747 645 524 200 91 39 27
EagI C'GGCC_G
Cuts at: 0 39 1329 3363
Size: 39 1290 2034
Fragments arranged by size:
2034 1290 39
EarI CTCTTCn'nnn_
Cuts at: 0 1442 2425 2521 2527 2843 2897 3209 3259
Size: 1442 983 96 6 316 54 312 50
Cuts at: 3259 3363
Size: 104
Fragments arranged by size:
1442 983 316 312 104 96 54 50
6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 838 984 1287 1466 1513 1807 1812
Size: 178 660 146 303 179 47 294 5
Cuts at: 1812 1821 2014 2247 3280 3363
Size: 9 193 233 1033 83
Fragments arranged by size:
1033 660 303 294 233 193 179 178
146 83 47 9 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2581 2788 3363
Size: 2581 207 575
Fragments arranged by size:
2581 575 207
EcoO109I rG'GnC_Cy
Cuts at: 0 27 102 1293 1535 1875 2367 2793 3027
Size: 27 75 1191 242 340 492 426 234
Cuts at: 3027 3363
Size: 336
Fragments arranged by size:
1191 492 426 340 336 242 234 75
27
EcoRI G'AATT_C
Cuts at: 0 1039 1265 3363
Size: 1039 226 2098
Fragments arranged by size:
2098 1039 226
FspI TGC'GCA
Cuts at: 0 2976 3363
Size: 2976 387
HaeII r_GCGC'y
Cuts at: 0 942 1808 2350 2553 2576 3363
Size: 942 866 542 203 23 787
Fragments arranged by size:
942 866 787 542 203 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 313 345 1335 1367 2359 2391 3043 3075
Size: 313 32 990 32 992 32 652 32
Cuts at: 3075 3363
Size: 288
Fragments arranged by size:
992 990 652 313 288 32 32 32
32
HincII GTy'rAC
Cuts at: 0 2361 3363
Size: 2361 1002
HindIII A'AGCT_T
Cuts at: 0 76 486 3101 3363
Size: 76 410 2615 262
Fragments arranged by size:
2615 410 262 76
KpnI G_GTAC'C
Cuts at: 0 1185 3363
Size: 1185 2178
MluI A'CGCG_T
Cuts at: 0 753 3363
Size: 753 2610
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 671 986 2171 3363
Size: 671 315 1185 1192
Fragments arranged by size:
1192 1185 671 315
MscI TGG'CCA
Cuts at: 0 1946 2693 3363
Size: 1946 747 670
Fragments arranged by size:
1946 747 670
MslI CAynn'nnrTG
Cuts at: 0 221 266 1570 3363
Size: 221 45 1304 1793
Fragments arranged by size:
1793 1304 221 45
MspA1I CmG'CkG
Cuts at: 0 156 473 542 1004 1130 1942 2566 2643
Size: 156 317 69 462 126 812 624 77
Cuts at: 2643 2650 2810 2892 2972 3363
Size: 7 160 82 80 391
Fragments arranged by size:
812 624 462 391 317 160 156 126
82 80 77 69 7
NcoI C'CATG_G
Cuts at: 0 2819 2843 3363
Size: 2819 24 520
Fragments arranged by size:
2819 520 24
NspI r_CATG'y
Cuts at: 0 466 498 903 1575 3363
Size: 466 32 405 672 1788
Fragments arranged by size:
1788 672 466 405 32
PasI CC'CwG_GG
Cuts at: 0 1485 2305 3363
Size: 1485 820 1058
Fragments arranged by size:
1485 1058 820
PciI A'CATG_T
Cuts at: 0 899 3363
Size: 899 2464
PflMI CCAn_nnn'nTGG
Cuts at: 0 1399 2700 3363
Size: 1399 1301 663
Fragments arranged by size:
1399 1301 663
PfoI T'CCnGG_A
Cuts at: 0 1303 1468 3071 3363
Size: 1303 165 1603 292
Fragments arranged by size:
1603 1303 292 165
PpuMI rG'GwC_Cy
Cuts at: 0 102 1535 2367 2793 3363
Size: 102 1433 832 426 570
Fragments arranged by size:
1433 832 570 426 102
PshAI GACnn'nnGTC
Cuts at: 0 2120 3363
Size: 2120 1243
PstI C_TGCA'G
Cuts at: 0 2582 3363
Size: 2582 781
PvuII CAG'CTG
Cuts at: 0 156 473 1130 2566 2643 2810 2972 3363
Size: 156 317 657 1436 77 167 162 391
Fragments arranged by size:
1436 657 391 317 167 162 156 77
SanDI GG'GwC_CC
Cuts at: 0 1535 3363
Size: 1535 1828
SbfI CC_TGCA'GG
Cuts at: 0 2582 3363
Size: 2582 781
SfcI C'TryA_G
Cuts at: 0 1125 1760 2578 3363
Size: 1125 635 818 785
Fragments arranged by size:
1125 818 785 635
SmlI C'TyrA_G
Cuts at: 0 982 3363
Size: 982 2381
SphI G_CATG'C
Cuts at: 0 466 498 3363
Size: 466 32 2865
Fragments arranged by size:
2865 466 32
SspI AAT'ATT
Cuts at: 0 840 3363
Size: 840 2523
StuI AGG'CCT
Cuts at: 0 163 529 1243 3363
Size: 163 366 714 2120
Fragments arranged by size:
2120 714 366 163
StyI C'CwwG_G
Cuts at: 0 378 455 716 1019 1199 1871 2819 2843
Size: 378 77 261 303 180 672 948 24
Cuts at: 2843 2857 2926 3363
Size: 14 69 437
Fragments arranged by size:
948 672 437 378 303 261 180 77
69 24 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1578 2170 2812 3239 3344 3363
Size: 1578 592 642 427 105 19
Fragments arranged by size:
1578 642 592 427 105 19
TatI w'GTAC_w
Cuts at: 0 2618 3363
Size: 2618 745
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 943 975 1589 1621 1736 1768 2028 2060
Size: 943 32 614 32 115 32 260 32
Cuts at: 2060 2499 2531 3363
Size: 439 32 832
Fragments arranged by size:
943 832 614 439 260 115 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 286 3363
Size: 286 3077
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 730 3363
Size: 730 2633
ZraI GAC'GTC
Cuts at: 0 810 2312 3363
Size: 810 1502 1051
Fragments arranged by size:
1502 1051 810
Enzymes that do cut:
AarI AatII Acc65I AcuI AfeI AflIII AhdI AleI
AlwNI ApaLI ApoI AvaI BaeI BanI BanII BbsI
BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI
BstAPI BstXI BstYI Bsu36I BtgI BtsI CspCI DraIII
EaeI EagI EarI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII KpnI MluI MmeI MscI
MslI MspA1I NcoI NspI PasI PciI PflMI PfoI
PpuMI PshAI PstI PvuII SanDI SbfI SfcI SmlI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XcmI ZraI
Enzymes that do not cut:
AccI AclI AflII AgeI AlfI AloI ApaI AscI
AseI AsiSI AvrII BamHI BbeI BciVI BclI BfrBI
BmtI BsiWI BspHI BssHII BssSI BstBI BstEII BstZ17I
BtgZI ClaI DraI DrdI EciI EcoICRI EcoRV FalI
FseI FspAI HpaI KasI MfeI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI
PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SapI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SwaI XbaI XhoI
XmaI XmnI