Restriction map for
pF1KSDA1456
(Linear) (Six-base) MAPSORT of: KIAA1456.seq Check: 4655 from: 1 to: 1101
KIAA1456.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
ApaI G_GGCC'C
Cuts at: 0 228 1101
Size: 228 873
ApoI r'AATT_y
Cuts at: 0 780 1101
Size: 780 321
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1053 1086 1101
Size: 1053 33 15
Fragments arranged by size:
1053 33 15
BanII G_rGCy'C
Cuts at: 0 228 998 1031 1101
Size: 228 770 33 70
Fragments arranged by size:
770 228 70 33
BciVI GTATCCnnnnn_n'
Cuts at: 0 277 1045 1101
Size: 277 768 56
Fragments arranged by size:
768 277 56
BclI T'GATC_A
Cuts at: 0 621 1053 1101
Size: 621 432 48
Fragments arranged by size:
621 432 48
BglII A'GATC_T
Cuts at: 0 499 1101
Size: 499 602
Bme1580I G_kGCm'C
Cuts at: 0 228 1101
Size: 228 873
BmgBI CAC'GTC
Cuts at: 0 204 1101
Size: 204 897
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 480 1101
Size: 480 621
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 994 1101
Size: 994 107
BsaI GGTCTCn'nnnn_
Cuts at: 0 737 1101
Size: 737 364
BsaBI GATnn'nnATC
Cuts at: 0 842 1101
Size: 842 259
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1006 1036 1101
Size: 1006 30 65
Fragments arranged by size:
1006 65 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 777 1101
Size: 777 324
BseYI C'CCAG_C
Cuts at: 0 236 1101
Size: 236 865
BsiHKAI G_wGCw'C
Cuts at: 0 717 998 1031 1101
Size: 717 281 33 70
Fragments arranged by size:
717 281 70 33
Bsp1286I G_dGCh'C
Cuts at: 0 228 717 998 1031 1101
Size: 228 489 281 33 70
Fragments arranged by size:
489 281 228 70 33
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 402 1101
Size: 402 699
BsrDI GCAATG_nn'
Cuts at: 0 173 1073 1101
Size: 173 900 28
Fragments arranged by size:
900 173 28
BstAPI GCAn_nnn'nTGC
Cuts at: 0 204 1101
Size: 204 897
BstXI CCAn_nnnn'nTGG
Cuts at: 0 504 1101
Size: 504 597
BstYI r'GATC_y
Cuts at: 0 499 927 1101
Size: 499 428 174
Fragments arranged by size:
499 428 174
BtgI C'CryG_G
Cuts at: 0 214 1101
Size: 214 887
BtsI GCAGTG_nn'
Cuts at: 0 283 1101
Size: 283 818
DraI TTT'AAA
Cuts at: 0 348 737 1101
Size: 348 389 364
Fragments arranged by size:
389 364 348
DraIII CAC_nnn'GTG
Cuts at: 0 381 1101
Size: 381 720
DrdI GACnn_nn'nnGTC
Cuts at: 0 608 1101
Size: 608 493
EaeI y'GGCC_r
Cuts at: 0 119 1101
Size: 119 982
EarI CTCTTCn'nnn_
Cuts at: 0 247 265 1101
Size: 247 18 836
Fragments arranged by size:
836 247 18
EcoICRI GAG'CTC
Cuts at: 0 996 1029 1101
Size: 996 33 72
Fragments arranged by size:
996 72 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 480 1101
Size: 480 621
EcoO109I rG'GnC_Cy
Cuts at: 0 224 225 1101
Size: 224 1 876
Fragments arranged by size:
876 224 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 223 1101
Size: 223 878
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 901 933 1006 1038 1101
Size: 901 32 73 32 63
Fragments arranged by size:
901 73 63 32 32
MscI TGG'CCA
Cuts at: 0 121 1101
Size: 121 980
MslI CAynn'nnrTG
Cuts at: 0 737 1101
Size: 737 364
NcoI C'CATG_G
Cuts at: 0 214 1101
Size: 214 887
PflMI CCAn_nnn'nTGG
Cuts at: 0 381 1101
Size: 381 720
PfoI T'CCnGG_A
Cuts at: 0 135 1101
Size: 135 966
PspOMI G'GGCC_C
Cuts at: 0 224 1101
Size: 224 877
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1026 1058 1101
Size: 1026 32 43
Fragments arranged by size:
1026 43 32
PstI C_TGCA'G
Cuts at: 0 882 1003 1101
Size: 882 121 98
Fragments arranged by size:
882 121 98
SacI G_AGCT'C
Cuts at: 0 998 1031 1101
Size: 998 33 70
Fragments arranged by size:
998 70 33
SapI GCTCTTCn'nnn_
Cuts at: 0 247 1101
Size: 247 854
SfcI C'TryA_G
Cuts at: 0 878 999 1101
Size: 878 121 102
Fragments arranged by size:
878 121 102
SmlI C'TyrA_G
Cuts at: 0 1009 1101
Size: 1009 92
SspI AAT'ATT
Cuts at: 0 423 863 1101
Size: 423 440 238
Fragments arranged by size:
440 423 238
StyI C'CwwG_G
Cuts at: 0 214 1101
Size: 214 887
SwaI ATTT'AAAT
Cuts at: 0 737 1101
Size: 737 364
XbaI T'CTAG_A
Cuts at: 0 612 1101
Size: 612 489
XmnI GAAnn'nnTTC
Cuts at: 0 873 1101
Size: 873 228
Enzymes that do cut:
ApaI ApoI BaeI BanII BciVI BclI BglII Bme1580I
BmgBI BpmI BpuEI BsaI BsaBI BsaXI BseRI BseYI
BsiHKAI Bsp1286I BspMI BsrDI BstAPI BstXI BstYI BtgI
BtsI DraI DraIII DrdI EaeI EarI EcoICRI Eco57MI
EcoO109I FalI Hin4I MscI MslI NcoI PflMI PfoI
PspOMI PsrI PstI SacI SapI SfcI SmlI SspI
StyI SwaI XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
AscI AseI AsiSI AvaI AvrII BamHI BanI BbeI
BbsI BbvCI BcgI BfrBI BglI BlpI BmrI BmtI
BplI Bpu10I BsaAI BsaHI BsaWI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI
EagI EciI EcoNI EcoRI EcoRV FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MspA1I NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PvuI PvuII RsrII
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
TaqII TatI TstI Tth111I XcmI XhoI XmaI ZraI