Restriction map for
pF1KA1457
(Linear) (Six-base) MAPSORT of: KIAA1457.seq Check: 936 from: 1 to: 4029
KIAA1457.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3593 3866 4029
Size: 3593 273 163
Fragments arranged by size:
3593 273 163
AccI GT'mk_AC
Cuts at: 0 1937 2264 3221 4029
Size: 1937 327 957 808
Fragments arranged by size:
1937 957 808 327
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3294 3580 3766 4029
Size: 3294 286 186 263
Fragments arranged by size:
3294 286 263 186
AflIII A'CryG_T
Cuts at: 0 371 1361 3254 4029
Size: 371 990 1893 775
Fragments arranged by size:
1893 990 775 371
AhdI GACnn_n'nnGTC
Cuts at: 0 1794 4029
Size: 1794 2235
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1013 1842 1845 3814 4029
Size: 1013 829 3 1969 215
Fragments arranged by size:
1969 1013 829 215 3
AlwNI CAG_nnn'CTG
Cuts at: 0 1242 2822 3039 3408 4029
Size: 1242 1580 217 369 621
Fragments arranged by size:
1580 1242 621 369 217
ApaI G_GGCC'C
Cuts at: 0 512 3065 3893 4024 4029
Size: 512 2553 828 131 5
Fragments arranged by size:
2553 828 512 131 5
ApaLI G'TGCA_C
Cuts at: 0 1384 3103 3592 4029
Size: 1384 1719 489 437
Fragments arranged by size:
1719 1384 489 437
ApoI r'AATT_y
Cuts at: 0 316 4029
Size: 316 3713
AvaI C'yCGr_G
Cuts at: 0 1800 2532 4029
Size: 1800 732 1497
Fragments arranged by size:
1800 1497 732
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3140 3173 4029
Size: 3140 33 856
Fragments arranged by size:
3140 856 33
BanI G'GyrC_C
Cuts at: 0 1171 1294 1312 1425 1916 2128 2177 2590
Size: 1171 123 18 113 491 212 49 413
Cuts at: 2590 4029
Size: 1439
Fragments arranged by size:
1439 1171 491 413 212 123 113 49
18
BanII G_rGCy'C
Cuts at: 0 512 669 809 851 860 881 1021 1102
Size: 512 157 140 42 9 21 140 81
Cuts at: 1102 1132 2084 3065 3893 4024 4029
Size: 30 952 981 828 131 5
Fragments arranged by size:
981 952 828 512 157 140 140 131
81 42 30 21 9 5
BbeI G_GCGC'C
Cuts at: 0 1316 2594 4029
Size: 1316 1278 1435
Fragments arranged by size:
1435 1316 1278
BbsI GAAGACnn'nnnn_
Cuts at: 0 543 1148 2215 3300 4029
Size: 543 605 1067 1085 729
Fragments arranged by size:
1085 1067 729 605 543
BbvCI CC'TCA_GC
Cuts at: 0 1474 2071 4029
Size: 1474 597 1958
Fragments arranged by size:
1958 1474 597
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3282 3316 4029
Size: 3282 34 713
Fragments arranged by size:
3282 713 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2013 3141 4029
Size: 2013 1128 888
Fragments arranged by size:
2013 1128 888
BglI GCCn_nnn'nGGC
Cuts at: 0 158 1760 2514 2776 3736 3824 3888 4029
Size: 158 1602 754 262 960 88 64 141
Fragments arranged by size:
1602 960 754 262 158 141 88 64
BglII A'GATC_T
Cuts at: 0 3659 4029
Size: 3659 370
BlpI GC'TnA_GC
Cuts at: 0 709 4029
Size: 709 3320
Bme1580I G_kGCm'C
Cuts at: 0 446 512 1176 1388 1430 2133 2182 2611
Size: 446 66 664 212 42 703 49 429
Cuts at: 2611 3065 3107 3295 3596 3893 4024 4029
Size: 454 42 188 301 297 131 5
Fragments arranged by size:
703 664 454 446 429 301 297 212
188 131 66 49 42 42 5
BmgBI CAC'GTC
Cuts at: 0 372 2838 3387 3621 4029
Size: 372 2466 549 234 408
Fragments arranged by size:
2466 549 408 372 234
BmrI ACTGGGnnnn_n'
Cuts at: 0 609 768 1560 2832 3095 4029
Size: 609 159 792 1272 263 934
Fragments arranged by size:
1272 934 792 609 263 159
BmtI G_CTAG'C
Cuts at: 0 3338 4029
Size: 3338 691
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 336 2103 4029
Size: 336 1767 1926
Fragments arranged by size:
1926 1767 336
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 377 754 1179 1881 1924 2326 2458
Size: 148 229 377 425 702 43 402 132
Cuts at: 2458 2542 2865 4021 4029
Size: 84 323 1156 8
Fragments arranged by size:
1156 702 425 402 377 323 229 148
132 84 43 8
Bpu10I CC'TnA_GC
Cuts at: 0 1474 1921 2071 2426 4029
Size: 1474 447 150 355 1603
Fragments arranged by size:
1603 1474 447 355 150
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 883 2223 4029
Size: 883 1340 1806
Fragments arranged by size:
1806 1340 883
BsaI GGTCTCn'nnnn_
Cuts at: 0 1470 2401 2847 3242 4029
Size: 1470 931 446 395 787
Fragments arranged by size:
1470 931 787 446 395
BsaAI yAC'GTr
Cuts at: 0 1269 2967 3429 4029
Size: 1269 1698 462 600
Fragments arranged by size:
1698 1269 600 462
BsaBI GATnn'nnATC
Cuts at: 0 2653 3664 4029
Size: 2653 1011 365
Fragments arranged by size:
2653 1011 365
BsaHI Gr'CG_yC
Cuts at: 0 1313 1451 2591 2768 4029
Size: 1313 138 1140 177 1261
Fragments arranged by size:
1313 1261 1140 177 138
BsaWI w'CCGG_w
Cuts at: 0 640 2996 4029
Size: 640 2356 1033
Fragments arranged by size:
2356 1033 640
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1312 1342 2827 2857 4029
Size: 1312 30 1485 30 1172
Fragments arranged by size:
1485 1312 1172 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 58 271 547 808 1548 2007 2589 4029
Size: 58 213 276 261 740 459 582 1440
Fragments arranged by size:
1440 740 582 459 276 261 213 58
BseYI C'CCAG_C
Cuts at: 0 59 208 500 859 1324 1393 2184 2220
Size: 59 149 292 359 465 69 791 36
Cuts at: 2220 2376 3116 3479 3878 3883 3892 3974 4029
Size: 156 740 363 399 5 9 82 55
Fragments arranged by size:
791 740 465 399 363 359 292 156
149 82 69 59 55 36 9 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1232 1270 2434 3565 4029
Size: 1232 38 1164 1131 464
Fragments arranged by size:
1232 1164 1131 464 38
BsiEI CG_ry'CG
Cuts at: 0 139 937 2248 3968 4029
Size: 139 798 1311 1720 61
Fragments arranged by size:
1720 1311 798 139 61
BsiHKAI G_wGCw'C
Cuts at: 0 809 1021 1102 1388 2558 3107 3596 4029
Size: 809 212 81 286 1170 549 489 433
Fragments arranged by size:
1170 809 549 489 433 286 212 81
BsmI GAATG_Cn'
Cuts at: 0 273 1821 4029
Size: 273 1548 2208
Fragments arranged by size:
2208 1548 273
BsmBI CGTCTCn'nnnn_
Cuts at: 0 831 935 4029
Size: 831 104 3094
Fragments arranged by size:
3094 831 104
Bsp1286I G_dGCh'C
Cuts at: 0 446 512 669 809 851 860 881 1021
Size: 446 66 157 140 42 9 21 140
Cuts at: 1021 1102 1132 1176 1388 1430 2084 2133 2182
Size: 81 30 44 212 42 654 49 49
Cuts at: 2182 2558 2611 3065 3107 3295 3596 3893 4024
Size: 376 53 454 42 188 301 297 131
Cuts at: 4024 4029
Size: 5
Fragments arranged by size:
654 454 446 376 301 297 212 188
157 140 140 131 81 66 53 49
49 44 42 42 42 30 21 9
5
BspHI T'CATG_A
Cuts at: 0 2864 3110 4029
Size: 2864 246 919
Fragments arranged by size:
2864 919 246
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 537 1650 1799 2129 3038 3593 3866 4029
Size: 537 1113 149 330 909 555 273 163
Fragments arranged by size:
1113 909 555 537 330 273 163 149
BsrBI CCG'CTC
Cuts at: 0 221 3772 3909 4029
Size: 221 3551 137 120
Fragments arranged by size:
3551 221 137 120
BsrDI GCAATG_nn'
Cuts at: 0 876 4029
Size: 876 3153
BsrFI r'CCGG_y
Cuts at: 0 134 671 1728 2247 3437 3674 3899 3967
Size: 134 537 1057 519 1190 237 225 68
Cuts at: 3967 4029
Size: 62
Fragments arranged by size:
1190 1057 537 519 237 225 134 68
62
BsrGI T'GTAC_A
Cuts at: 0 65 3629 4029
Size: 65 3564 400
Fragments arranged by size:
3564 400 65
BssSI C'ACGA_G
Cuts at: 0 1045 3904 4029
Size: 1045 2859 125
Fragments arranged by size:
2859 1045 125
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1705 4029
Size: 1705 2324
BstEII G'GTnAC_C
Cuts at: 0 3075 3156 4029
Size: 3075 81 873
Fragments arranged by size:
3075 873 81
BstXI CCAn_nnnn'nTGG
Cuts at: 0 841 1401 1422 2115 2400 2587 2889 3621
Size: 841 560 21 693 285 187 302 732
Cuts at: 3621 3792 3928 4029
Size: 171 136 101
Fragments arranged by size:
841 732 693 560 302 285 187 171
136 101 21
BstYI r'GATC_y
Cuts at: 0 122 1262 3659 4029
Size: 122 1140 2397 370
Fragments arranged by size:
2397 1140 370 122
Bsu36I CC'TnA_GG
Cuts at: 0 3126 4029
Size: 3126 903
BtgI C'CryG_G
Cuts at: 0 38 2282 2466 2786 3380 3495 4016 4029
Size: 38 2244 184 320 594 115 521 13
Fragments arranged by size:
2244 594 521 320 184 115 38 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 39 1338 1355 2640 2688 4029
Size: 39 1299 17 1285 48 1341
Fragments arranged by size:
1341 1299 1285 48 39 17
BtsI GCAGTG_nn'
Cuts at: 0 1824 1875 2609 3706 4029
Size: 1824 51 734 1097 323
Fragments arranged by size:
1824 1097 734 323 51
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 20 55 2819 2854 3150 3185 4029
Size: 20 35 2764 35 296 35 844
Fragments arranged by size:
2764 844 296 35 35 35 20
DraIII CAC_nnn'GTG
Cuts at: 0 183 1094 4029
Size: 183 911 2935
Fragments arranged by size:
2935 911 183
EaeI y'GGCC_r
Cuts at: 0 136 1550 2245 2987 3782 3825 3965 3993
Size: 136 1414 695 742 795 43 140 28
Cuts at: 3993 4029
Size: 36
Fragments arranged by size:
1414 795 742 695 140 136 43 36
28
EagI C'GGCC_G
Cuts at: 0 136 2245 3965 4029
Size: 136 2109 1720 64
Fragments arranged by size:
2109 1720 136 64
EarI CTCTTCn'nnn_
Cuts at: 0 76 461 2170 2176 2278 2812 4029
Size: 76 385 1709 6 102 534 1217
Fragments arranged by size:
1709 1217 534 385 102 76 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1408 2623 3940 4029
Size: 1408 1215 1317 89
Fragments arranged by size:
1408 1317 1215 89
EcoICRI GAG'CTC
Cuts at: 0 807 1019 1100 4029
Size: 807 212 81 2929
Fragments arranged by size:
2929 807 212 81
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 377 754 1179 1881 1924 2326 2458
Size: 148 229 377 425 702 43 402 132
Cuts at: 2458 2542 2865 3294 3580 3766 4021 4029
Size: 84 323 429 286 186 255 8
Fragments arranged by size:
702 429 425 402 377 323 286 255
229 186 148 132 84 43 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 232 4029
Size: 232 3797
EcoO109I rG'GnC_Cy
Cuts at: 0 471 508 509 1050 1320 1965 3062 3405
Size: 471 37 1 541 270 645 1097 343
Cuts at: 3405 4021 4029
Size: 616 8
Fragments arranged by size:
1097 645 616 541 471 343 270 37
8 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 567 3570 3828 4029
Size: 567 3003 258 201
Fragments arranged by size:
3003 567 258 201
FseI GG_CCGG'CC
Cuts at: 0 2251 3441 3678 4029
Size: 2251 1190 237 351
Fragments arranged by size:
2251 1190 351 237
FspI TGC'GCA
Cuts at: 0 567 1043 3802 4029
Size: 567 476 2759 227
Fragments arranged by size:
2759 567 476 227
FspAI rTGC'GCAy
Cuts at: 0 567 1043 4029
Size: 567 476 2986
Fragments arranged by size:
2986 567 476
HaeII r_GCGC'y
Cuts at: 0 1316 1400 2328 2594 3638 3799 4029
Size: 1316 84 928 266 1044 161 230
Fragments arranged by size:
1316 1044 928 266 230 161 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 823 855 1003 1035 1039 1071 1283 1312
Size: 823 32 148 32 4 32 212 29
Cuts at: 1312 1315 1344 1780 1812 2374 2406 2827 2859
Size: 3 29 436 32 562 32 421 32
Cuts at: 2859 4029
Size: 1170
Fragments arranged by size:
1170 823 562 436 421 212 148 32
32 32 32 32 32 29 29 4
3
HincII GTy'rAC
Cuts at: 0 491 1938 2831 4029
Size: 491 1447 893 1198
Fragments arranged by size:
1447 1198 893 491
KasI G'GCGC_C
Cuts at: 0 1312 2590 4029
Size: 1312 1278 1439
Fragments arranged by size:
1439 1312 1278
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1082 1493 2528 4029
Size: 1082 411 1035 1501
Fragments arranged by size:
1501 1082 1035 411
MscI TGG'CCA
Cuts at: 0 1552 4029
Size: 1552 2477
MslI CAynn'nnrTG
Cuts at: 0 1375 1381 1810 3277 3427 3790 3958 4029
Size: 1375 6 429 1467 150 363 168 71
Fragments arranged by size:
1467 1375 429 363 168 150 71 6
MspA1I CmG'CkG
Cuts at: 0 63 113 212 405 504 1209 1242 2184
Size: 63 50 99 193 99 705 33 942
Cuts at: 2184 2241 2284 2313 2376 3459 3537 3744 3887
Size: 57 43 29 63 1083 78 207 143
Cuts at: 3887 3939 4018 4029
Size: 52 79 11
Fragments arranged by size:
1083 942 705 207 193 143 99 99
79 78 63 63 57 52 50 43
33 29 11
NaeI GCC'GGC
Cuts at: 0 2249 3439 3676 3969 4029
Size: 2249 1190 237 293 60
Fragments arranged by size:
2249 1190 293 237 60
NarI GG'CG_CC
Cuts at: 0 1313 2591 4029
Size: 1313 1278 1438
Fragments arranged by size:
1438 1313 1278
NcoI C'CATG_G
Cuts at: 0 3495 4029
Size: 3495 534
NdeI CA'TA_TG
Cuts at: 0 99 4029
Size: 99 3930
NgoMIV G'CCGG_C
Cuts at: 0 2247 3437 3674 3967 4029
Size: 2247 1190 237 293 62
Fragments arranged by size:
2247 1190 293 237 62
NheI G'CTAG_C
Cuts at: 0 3334 4029
Size: 3334 695
NspI r_CATG'y
Cuts at: 0 201 609 1779 3450 4029
Size: 201 408 1170 1671 579
Fragments arranged by size:
1671 1170 579 408 201
PasI CC'CwG_GG
Cuts at: 0 1401 2133 4029
Size: 1401 732 1896
Fragments arranged by size:
1896 1401 732
PflMI CCAn_nnn'nTGG
Cuts at: 0 1242 2822 3501 4029
Size: 1242 1580 679 528
Fragments arranged by size:
1580 1242 679 528
PfoI T'CCnGG_A
Cuts at: 0 125 725 1301 2435 2480 3847 4029
Size: 125 600 576 1134 45 1367 182
Fragments arranged by size:
1367 1134 600 576 182 125 45
PmlI CAC'GTG
Cuts at: 0 1269 2967 4029
Size: 1269 1698 1062
Fragments arranged by size:
1698 1269 1062
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1057 1089 4029
Size: 1057 32 2940
Fragments arranged by size:
2940 1057 32
PpuMI rG'GwC_Cy
Cuts at: 0 471 1050 1320 1965 3405 4029
Size: 471 579 270 645 1440 624
Fragments arranged by size:
1440 645 624 579 471 270
PsiI TTA'TAA
Cuts at: 0 7 344 4029
Size: 7 337 3685
Fragments arranged by size:
3685 337 7
PspOMI G'GGCC_C
Cuts at: 0 508 3061 3889 4020 4029
Size: 508 2553 828 131 9
Fragments arranged by size:
2553 828 508 131 9
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 124 156 4029
Size: 124 32 3873
Fragments arranged by size:
3873 124 32
PstI C_TGCA'G
Cuts at: 0 3695 4029
Size: 3695 334
PvuII CAG'CTG
Cuts at: 0 63 212 405 1209 2241 3744 4029
Size: 63 149 193 804 1032 1503 285
Fragments arranged by size:
1503 1032 804 285 193 149 63
SacI G_AGCT'C
Cuts at: 0 809 1021 1102 4029
Size: 809 212 81 2927
Fragments arranged by size:
2927 809 212 81
SacII CC_GC'GG
Cuts at: 0 2285 4019 4029
Size: 2285 1734 10
Fragments arranged by size:
2285 1734 10
SalI G'TCGA_C
Cuts at: 0 1936 4029
Size: 1936 2093
SanDI GG'GwC_CC
Cuts at: 0 1320 4029
Size: 1320 2709
SapI GCTCTTCn'nnn_
Cuts at: 0 76 4029
Size: 76 3953
SexAI A'CCwGG_T
Cuts at: 0 289 4029
Size: 289 3740
SfcI C'TryA_G
Cuts at: 0 1482 1716 3691 4029
Size: 1482 234 1975 338
Fragments arranged by size:
1975 1482 338 234
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3824 4029
Size: 3824 205
SfoI GGC'GCC
Cuts at: 0 1314 2592 4029
Size: 1314 1278 1437
Fragments arranged by size:
1437 1314 1278
SmaI CCC'GGG
Cuts at: 0 1802 2534 4029
Size: 1802 732 1495
Fragments arranged by size:
1802 1495 732
SmlI C'TyrA_G
Cuts at: 0 898 2202 4029
Size: 898 1304 1827
Fragments arranged by size:
1827 1304 898
SphI G_CATG'C
Cuts at: 0 201 609 1779 4029
Size: 201 408 1170 2250
Fragments arranged by size:
2250 1170 408 201
SrfI GCCC'GGGC
Cuts at: 0 2534 4029
Size: 2534 1495
StuI AGG'CCT
Cuts at: 0 679 2041 4029
Size: 679 1362 1988
Fragments arranged by size:
1988 1362 679
StyI C'CwwG_G
Cuts at: 0 233 1079 2935 3287 3362 3495 3614 4029
Size: 233 846 1856 352 75 133 119 415
Fragments arranged by size:
1856 846 415 352 233 133 119 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 923 3205 4029
Size: 923 2282 824
Fragments arranged by size:
2282 923 824
TatI w'GTAC_w
Cuts at: 0 65 170 536 2756 3629 3749 4029
Size: 65 105 366 2220 873 120 280
Fragments arranged by size:
2220 873 366 280 120 105 65
Tth111I GACn'n_nGTC
Cuts at: 0 427 1372 1681 2839 3073 4029
Size: 427 945 309 1158 234 956
Fragments arranged by size:
1158 956 945 427 309 234
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1110 2013 2942 3618 4029
Size: 1110 903 929 676 411
Fragments arranged by size:
1110 929 903 676 411
XmaI C'CCGG_G
Cuts at: 0 1800 2532 4029
Size: 1800 732 1497
Fragments arranged by size:
1800 1497 732
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AlfI AlwNI ApaI
ApaLI ApoI AvaI BaeI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI FspI
FspAI HaeII Hin4I HincII KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NspI
PasI PflMI PfoI PmlI PpiI PpuMI PsiI PspOMI
PsrI PstI PvuII SacI SacII SalI SanDI SapI
SexAI SfcI SfiI SfoI SmaI SmlI SphI SrfI
StuI StyI TaqII TatI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AleI AloI
AscI AseI AsiSI AvrII BamHI BclI BfrBI BsiWI
BspEI BssHII BstBI BstZ17I ClaI DraI DrdI EcoRI
EcoRV HindIII HpaI KpnI MfeI MluI NotI NruI
NsiI PacI PciI PmeI PshAI PspXI PvuI RsrII
SbfI ScaI SgfI SgrAI SnaBI SpeI SspI SwaI
TstI XbaI XhoI XmnI ZraI