Restriction map for
pF1KA1462
(Linear) (Six-base) MAPSORT of: KIAA1462.seq Check: 2010 from: 1 to: 4077
KIAA1462.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1660 4077
Size: 1660 2417
AatII G_ACGT'C
Cuts at: 0 178 4077
Size: 178 3899
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1145 3618 3810 4077
Size: 1145 2473 192 267
Fragments arranged by size:
2473 1145 267 192
AflIII A'CryG_T
Cuts at: 0 192 654 4077
Size: 192 462 3423
Fragments arranged by size:
3423 462 192
AgeI A'CCGG_T
Cuts at: 0 2051 2212 2466 4077
Size: 2051 161 254 1611
Fragments arranged by size:
2051 1611 254 161
AhdI GACnn_n'nnGTC
Cuts at: 0 3144 4077
Size: 3144 933
AleI CACnn'nnGTG
Cuts at: 0 2774 4077
Size: 2774 1303
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2850 3369 4077
Size: 2850 519 708
Fragments arranged by size:
2850 708 519
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3580 3612 4077
Size: 3580 32 465
Fragments arranged by size:
3580 465 32
AlwNI CAG_nnn'CTG
Cuts at: 0 126 532 997 1869 2173 3559 3685 3766
Size: 126 406 465 872 304 1386 126 81
Cuts at: 3766 4077
Size: 311
Fragments arranged by size:
1386 872 465 406 311 304 126 126
81
ApaI G_GGCC'C
Cuts at: 0 2425 4077
Size: 2425 1652
ApaLI G'TGCA_C
Cuts at: 0 2825 4077
Size: 2825 1252
ApoI r'AATT_y
Cuts at: 0 663 734 801 819 4077
Size: 663 71 67 18 3258
Fragments arranged by size:
3258 663 71 67 18
AvaI C'yCGr_G
Cuts at: 0 712 1513 2793 3894 4077
Size: 712 801 1280 1101 183
Fragments arranged by size:
1280 1101 801 712 183
AvrII C'CTAG_G
Cuts at: 0 2986 3751 4077
Size: 2986 765 326
Fragments arranged by size:
2986 765 326
BamHI G'GATC_C
Cuts at: 0 3263 4077
Size: 3263 814
BanI G'GyrC_C
Cuts at: 0 86 1083 1414 2048 2319 2691 3178 4037
Size: 86 997 331 634 271 372 487 859
Cuts at: 4037 4077
Size: 40
Fragments arranged by size:
997 859 634 487 372 331 271 86
40
BanII G_rGCy'C
Cuts at: 0 245 983 1201 1220 1849 2096 2379 2417
Size: 245 738 218 19 629 247 283 38
Cuts at: 2417 2425 2525 2704 2996 3095 3128 3158 3341
Size: 8 100 179 292 99 33 30 183
Cuts at: 3341 3469 3507 3578 3799 3893 4077
Size: 128 38 71 221 94 184
Fragments arranged by size:
738 629 292 283 247 245 221 218
184 183 179 128 100 99 94 71
38 38 33 30 19 8
BbeI G_GCGC'C
Cuts at: 0 90 4077
Size: 90 3987
BbsI GAAGACnn'nnnn_
Cuts at: 0 20 1082 1337 1620 1874 1976 2927 4077
Size: 20 1062 255 283 254 102 951 1150
Fragments arranged by size:
1150 1062 951 283 255 254 102 20
BbvCI CC'TCA_GC
Cuts at: 0 300 2266 4077
Size: 300 1966 1811
Fragments arranged by size:
1966 1811 300
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3331 3365 4077
Size: 3331 34 712
Fragments arranged by size:
3331 712 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 28 791 4077
Size: 28 763 3286
Fragments arranged by size:
3286 763 28
BclI T'GATC_A
Cuts at: 0 1821 2217 2492 4077
Size: 1821 396 275 1585
Fragments arranged by size:
1821 1585 396 275
BglI GCCn_nnn'nGGC
Cuts at: 0 1215 3502 4077
Size: 1215 2287 575
Fragments arranged by size:
2287 1215 575
BglII A'GATC_T
Cuts at: 0 1950 4077
Size: 1950 2127
BlpI GC'TnA_GC
Cuts at: 0 840 1310 1873 2838 3335 4077
Size: 840 470 563 965 497 742
Fragments arranged by size:
965 840 742 563 497 470
Bme1580I G_kGCm'C
Cuts at: 0 1088 1607 2051 2241 2425 2829 3933 4042
Size: 1088 519 444 190 184 404 1104 109
Cuts at: 4042 4077
Size: 35
Fragments arranged by size:
1104 1088 519 444 404 190 184 109
35
BmrI ACTGGGnnnn_n'
Cuts at: 0 113 1187 1697 1750 1808 4077
Size: 113 1074 510 53 58 2269
Fragments arranged by size:
2269 1074 510 113 58 53
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1868 3813 4077
Size: 1868 1945 264
Fragments arranged by size:
1945 1868 264
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 995 1347 1915 2509 2766 2896 3119
Size: 421 574 352 568 594 257 130 223
Cuts at: 3119 3960 4077
Size: 841 117
Fragments arranged by size:
841 594 574 568 421 352 257 223
130 117
Bpu10I CC'TnA_GC
Cuts at: 0 300 2266 2390 3566 3572 3820 4077
Size: 300 1966 124 1176 6 248 257
Fragments arranged by size:
1966 1176 300 257 248 124 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 862 1364 2008 2277 4077
Size: 862 502 644 269 1800
Fragments arranged by size:
1800 862 644 502 269
BsaI GGTCTCn'nnnn_
Cuts at: 0 44 1544 4077
Size: 44 1500 2533
Fragments arranged by size:
2533 1500 44
BsaAI yAC'GTr
Cuts at: 0 444 1020 2776 4077
Size: 444 576 1756 1301
Fragments arranged by size:
1756 1301 576 444
BsaHI Gr'CG_yC
Cuts at: 0 87 175 959 2326 3851 4077
Size: 87 88 784 1367 1525 226
Fragments arranged by size:
1525 1367 784 226 88 87
BsaWI w'CCGG_w
Cuts at: 0 344 2051 2212 2466 3308 4077
Size: 344 1707 161 254 842 769
Fragments arranged by size:
1707 842 769 344 254 161
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 265 295 604 634 641 671 3580 3610
Size: 265 30 309 30 7 30 2909 30
Cuts at: 3610 3890 3920 3958 3988 4077
Size: 280 30 38 30 89
Fragments arranged by size:
2909 309 280 265 89 38 30 30
30 30 30 30 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 932 2104 2593 2767 3497 4077
Size: 932 1172 489 174 730 580
Fragments arranged by size:
1172 932 730 580 489 174
BseYI C'CCAG_C
Cuts at: 0 57 1138 2127 3393 3577 4077
Size: 57 1081 989 1266 184 500
Fragments arranged by size:
1266 1081 989 500 184 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2165 2879 3339 4077
Size: 2165 714 460 738
Fragments arranged by size:
2165 738 714 460
BsiEI CG_ry'CG
Cuts at: 0 1130 4077
Size: 1130 2947
BsiHKAI G_wGCw'C
Cuts at: 0 253 392 983 1651 2525 2616 2829 3524
Size: 253 139 591 668 874 91 213 695
Cuts at: 3524 3799 4077
Size: 275 278
Fragments arranged by size:
874 695 668 591 278 275 253 213
139 91
BsmI GAATG_Cn'
Cuts at: 0 1406 2028 3837 4077
Size: 1406 622 1809 240
Fragments arranged by size:
1809 1406 622 240
Bsp1286I G_dGCh'C
Cuts at: 0 245 253 392 983 1088 1201 1220 1607
Size: 245 8 139 591 105 113 19 387
Cuts at: 1607 1651 1849 2051 2096 2241 2379 2417 2425
Size: 44 198 202 45 145 138 38 8
Cuts at: 2425 2525 2616 2704 2829 2996 3095 3128 3158
Size: 100 91 88 125 167 99 33 30
Cuts at: 3158 3341 3469 3507 3524 3578 3799 3893 3933
Size: 183 128 38 17 54 221 94 40
Cuts at: 3933 4042 4077
Size: 109 35
Fragments arranged by size:
591 387 245 221 202 198 183 167
145 139 138 128 125 113 109 105
100 99 94 91 88 54 45 44
40 38 38 35 33 30 19 17
8 8
BspEI T'CCGG_A
Cuts at: 0 3308 4077
Size: 3308 769
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1660 2896 4077
Size: 1660 1236 1181
Fragments arranged by size:
1660 1236 1181
BsrBI CCG'CTC
Cuts at: 0 922 3316 4077
Size: 922 2394 761
Fragments arranged by size:
2394 922 761
BsrFI r'CCGG_y
Cuts at: 0 1386 2051 2212 2466 3399 4077
Size: 1386 665 161 254 933 678
Fragments arranged by size:
1386 933 678 665 254 161
BsrGI T'GTAC_A
Cuts at: 0 2 4077
Size: 2 4075
BssSI C'ACGA_G
Cuts at: 0 110 813 2853 4077
Size: 110 703 2040 1224
Fragments arranged by size:
2040 1224 703 110
BstBI TT'CG_AA
Cuts at: 0 3602 4077
Size: 3602 475
BstXI CCAn_nnnn'nTGG
Cuts at: 0 414 1893 2371 2780 3585 4077
Size: 414 1479 478 409 805 492
Fragments arranged by size:
1479 805 492 478 414 409
BstYI r'GATC_y
Cuts at: 0 1950 3263 4077
Size: 1950 1313 814
Fragments arranged by size:
1950 1313 814
Bsu36I CC'TnA_GG
Cuts at: 0 1211 3509 4077
Size: 1211 2298 568
Fragments arranged by size:
2298 1211 568
BtgI C'CryG_G
Cuts at: 0 179 234 457 1285 2309 2364 2417 2643
Size: 179 55 223 828 1024 55 53 226
Cuts at: 2643 4077
Size: 1434
Fragments arranged by size:
1434 1024 828 226 223 179 55 55
53
BtsI GCAGTG_nn'
Cuts at: 0 691 936 1241 1373 2580 2616 2959 3924
Size: 691 245 305 132 1207 36 343 965
Cuts at: 3924 4077
Size: 153
Fragments arranged by size:
1207 965 691 343 305 245 153 132
36
DraI TTT'AAA
Cuts at: 0 1302 4077
Size: 1302 2775
DrdI GACnn_nn'nnGTC
Cuts at: 0 964 2304 2931 3541 4077
Size: 964 1340 627 610 536
Fragments arranged by size:
1340 964 627 610 536
EaeI y'GGCC_r
Cuts at: 0 1475 2041 2766 4077
Size: 1475 566 725 1311
Fragments arranged by size:
1475 1311 725 566
EarI CTCTTCn'nnn_
Cuts at: 0 345 823 2525 2715 3371 3977 4009 4077
Size: 345 478 1702 190 656 606 32 68
Fragments arranged by size:
1702 656 606 478 345 190 68 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1016 2605 4077
Size: 1016 1589 1472
Fragments arranged by size:
1589 1472 1016
EcoICRI GAG'CTC
Cuts at: 0 981 2523 3797 4077
Size: 981 1542 1274 280
Fragments arranged by size:
1542 1274 981 280
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 995 1145 1347 1915 2509 2766 2896
Size: 421 574 150 202 568 594 257 130
Cuts at: 2896 3119 3618 3810 3960 4077
Size: 223 499 192 150 117
Fragments arranged by size:
594 574 568 499 421 257 223 202
192 150 150 130 117
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1160 4077
Size: 1160 2917
EcoO109I rG'GnC_Cy
Cuts at: 0 123 529 872 1165 1905 2261 2422 4077
Size: 123 406 343 293 740 356 161 1655
Fragments arranged by size:
1655 740 406 356 343 293 161 123
EcoRI G'AATT_C
Cuts at: 0 663 819 4077
Size: 663 156 3258
Fragments arranged by size:
3258 663 156
FspI TGC'GCA
Cuts at: 0 795 4077
Size: 795 3282
HaeII r_GCGC'y
Cuts at: 0 90 4077
Size: 90 3987
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 438 470 1336 1368 1454 1486 2719 2751
Size: 438 32 866 32 86 32 1233 32
Cuts at: 2751 3130 3162 3370 3402 3958 3990 4008 4040
Size: 379 32 208 32 556 32 18 32
Cuts at: 4040 4077
Size: 37
Fragments arranged by size:
1233 866 556 438 379 208 86 37
32 32 32 32 32 32 32 32
18
HincII GTy'rAC
Cuts at: 0 1114 4077
Size: 1114 2963
HindIII A'AGCT_T
Cuts at: 0 1339 4077
Size: 1339 2738
KasI G'GCGC_C
Cuts at: 0 86 4077
Size: 86 3991
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 447 1150 4077
Size: 447 703 2927
Fragments arranged by size:
2927 703 447
MscI TGG'CCA
Cuts at: 0 2043 2768 4077
Size: 2043 725 1309
Fragments arranged by size:
2043 1309 725
MslI CAynn'nnrTG
Cuts at: 0 143 197 1093 2774 3277 3527 4077
Size: 143 54 896 1681 503 250 550
Fragments arranged by size:
1681 896 550 503 250 143 54
MspA1I CmG'CkG
Cuts at: 0 181 1148 1556 2109 2645 2980 4077
Size: 181 967 408 553 536 335 1097
Fragments arranged by size:
1097 967 553 536 408 335 181
NaeI GCC'GGC
Cuts at: 0 1388 4077
Size: 1388 2689
NarI GG'CG_CC
Cuts at: 0 87 4077
Size: 87 3990
NcoI C'CATG_G
Cuts at: 0 457 4077
Size: 457 3620
NgoMIV G'CCGG_C
Cuts at: 0 1386 4077
Size: 1386 2691
NspI r_CATG'y
Cuts at: 0 196 487 658 1881 4077
Size: 196 291 171 1223 2196
Fragments arranged by size:
2196 1223 291 196 171
PasI CC'CwG_GG
Cuts at: 0 1317 2383 2483 3163 3413 4077
Size: 1317 1066 100 680 250 664
Fragments arranged by size:
1317 1066 680 664 250 100
PciI A'CATG_T
Cuts at: 0 192 654 4077
Size: 192 462 3423
Fragments arranged by size:
3423 462 192
PflMI CCAn_nnn'nTGG
Cuts at: 0 954 3939 4077
Size: 954 2985 138
Fragments arranged by size:
2985 954 138
PfoI T'CCnGG_A
Cuts at: 0 2525 4077
Size: 2525 1552
PmlI CAC'GTG
Cuts at: 0 444 2776 4077
Size: 444 2332 1301
Fragments arranged by size:
2332 1301 444
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3580 3612 4077
Size: 3580 32 465
Fragments arranged by size:
3580 465 32
PpuMI rG'GwC_Cy
Cuts at: 0 529 2261 4077
Size: 529 1732 1816
Fragments arranged by size:
1816 1732 529
PspOMI G'GGCC_C
Cuts at: 0 2421 4077
Size: 2421 1656
PstI C_TGCA'G
Cuts at: 0 131 1904 2623 2762 4077
Size: 131 1773 719 139 1315
Fragments arranged by size:
1773 1315 719 139 131
PvuII CAG'CTG
Cuts at: 0 2109 2980 4077
Size: 2109 871 1097
Fragments arranged by size:
2109 1097 871
SacI G_AGCT'C
Cuts at: 0 983 2525 3799 4077
Size: 983 1542 1274 278
Fragments arranged by size:
1542 1274 983 278
SacII CC_GC'GG
Cuts at: 0 182 2646 4077
Size: 182 2464 1431
Fragments arranged by size:
2464 1431 182
SapI GCTCTTCn'nnn_
Cuts at: 0 4009 4077
Size: 4009 68
SfcI C'TryA_G
Cuts at: 0 127 1900 2619 2758 4077
Size: 127 1773 719 139 1319
Fragments arranged by size:
1773 1319 719 139 127
SfoI GGC'GCC
Cuts at: 0 88 4077
Size: 88 3989
SmaI CCC'GGG
Cuts at: 0 1515 2795 3896 4077
Size: 1515 1280 1101 181
Fragments arranged by size:
1515 1280 1101 181
SmlI C'TyrA_G
Cuts at: 0 877 1379 2023 2292 4077
Size: 877 502 644 269 1785
Fragments arranged by size:
1785 877 644 502 269
StuI AGG'CCT
Cuts at: 0 1466 3902 4077
Size: 1466 2436 175
Fragments arranged by size:
2436 1466 175
StyI C'CwwG_G
Cuts at: 0 80 457 574 2986 3584 3751 3962 4077
Size: 80 377 117 2412 598 167 211 115
Fragments arranged by size:
2412 598 377 211 167 117 115 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1144 1912 2679 2786 3193 4077
Size: 1144 768 767 107 407 884
Fragments arranged by size:
1144 884 768 767 407 107
TatI w'GTAC_w
Cuts at: 0 2 1265 4077
Size: 2 1263 2812
Fragments arranged by size:
2812 1263 2
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 641 673 720 752 3890 3922 4077
Size: 641 32 47 32 3138 32 155
Fragments arranged by size:
3138 641 155 47 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 3559 4077
Size: 3559 518
XmaI C'CCGG_G
Cuts at: 0 1513 2793 3894 4077
Size: 1513 1280 1101 183
Fragments arranged by size:
1513 1280 1101 183
XmnI GAAnn'nnTTC
Cuts at: 0 2934 3705 4077
Size: 2934 771 372
Fragments arranged by size:
2934 771 372
ZraI GAC'GTC
Cuts at: 0 176 4077
Size: 176 3901
Enzymes that do cut:
AarI AatII AcuI AflIII AgeI AhdI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI
BsrFI BsrGI BssSI BstBI BstXI BstYI Bsu36I BtgI
BtsI DraI DrdI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
KasI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmlI PpiI
PpuMI PspOMI PstI PvuII SacI SacII SapI SfcI
SfoI SmaI SmlI StuI StyI TaqII TatI TstI
Tth111I XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AscI AseI AsiSI
BaeI BfrBI BmgBI BmtI BsaBI BsiWI BsmBI BspHI
BsrDI BssHII BstAPI BstEII BstZ17I BtgZI ClaI CspCI
DraIII EagI EcoRV FalI FseI FspAI HpaI KpnI
MfeI MluI NdeI NheI NotI NruI NsiI PacI
PmeI PshAI PsiI PspXI PsrI PvuI RsrII SalI
SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI XbaI XcmI XhoI