Restriction map for
pF1KSDA1465
(Linear) (Six-base) MAPSORT of: KIAA1465.seq Check: 421 from: 1 to: 2235
KIAA1465.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AatII G_ACGT'C
Cuts at: 0 221 2235
Size: 221 2014
AccI GT'mk_AC
Cuts at: 0 1055 2235
Size: 1055 1180
Acc65I G'GTAC_C
Cuts at: 0 1681 2235
Size: 1681 554
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 805 2235
Size: 805 1430
AfeI AGC'GCT
Cuts at: 0 1372 2235
Size: 1372 863
AflIII A'CryG_T
Cuts at: 0 1104 1412 2235
Size: 1104 308 823
Fragments arranged by size:
1104 823 308
AgeI A'CCGG_T
Cuts at: 0 855 2235
Size: 855 1380
AhdI GACnn_n'nnGTC
Cuts at: 0 167 2235
Size: 167 2068
AleI CACnn'nnGTG
Cuts at: 0 598 2235
Size: 598 1637
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 728 763 2235
Size: 728 35 1472
Fragments arranged by size:
1472 728 35
ApaI G_GGCC'C
Cuts at: 0 18 1130 1531 1538 2235
Size: 18 1112 401 7 697
Fragments arranged by size:
1112 697 401 18 7
ApaLI G'TGCA_C
Cuts at: 0 689 1067 2235
Size: 689 378 1168
Fragments arranged by size:
1168 689 378
AseI AT'TA_AT
Cuts at: 0 2207 2235
Size: 2207 28
AvaI C'yCGr_G
Cuts at: 0 416 596 610 1237 1282 1564 1860 1954
Size: 416 180 14 627 45 282 296 94
Cuts at: 1954 2235
Size: 281
Fragments arranged by size:
627 416 296 282 281 180 94 45
14
BanI G'GyrC_C
Cuts at: 0 132 486 493 657 739 835 972 1023
Size: 132 354 7 164 82 96 137 51
Cuts at: 1023 1084 1147 1681 1828 2176 2235
Size: 61 63 534 147 348 59
Fragments arranged by size:
534 354 348 164 147 137 132 96
82 63 61 59 51 7
BanII G_rGCy'C
Cuts at: 0 18 409 617 1130 1436 1531 1538 1557
Size: 18 391 208 513 306 95 7 19
Cuts at: 1557 1760 2149 2235
Size: 203 389 86
Fragments arranged by size:
513 391 389 306 208 203 95 86
19 18 7
BbeI G_GCGC'C
Cuts at: 0 490 976 1088 1151 1832 2180 2235
Size: 490 486 112 63 681 348 55
Fragments arranged by size:
681 490 486 348 112 63 55
BbsI GAAGACnn'nnnn_
Cuts at: 0 1343 1440 2042 2235
Size: 1343 97 602 193
Fragments arranged by size:
1343 602 193 97
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 607 641 1924 1958 2235
Size: 607 34 1283 34 277
Fragments arranged by size:
1283 607 277 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1045 2235
Size: 1045 1190
BlpI GC'TnA_GC
Cuts at: 0 508 2235
Size: 508 1727
Bme1580I G_kGCm'C
Cuts at: 0 18 693 1028 1071 1130 1531 1538 1820
Size: 18 675 335 43 59 401 7 282
Cuts at: 1820 2235
Size: 415
Fragments arranged by size:
675 415 401 335 282 59 43 18
7
BmgBI CAC'GTC
Cuts at: 0 233 1446 2013 2235
Size: 233 1213 567 222
Fragments arranged by size:
1213 567 233 222
BmrI ACTGGGnnnn_n'
Cuts at: 0 2106 2235
Size: 2106 129
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 636 2163 2235
Size: 636 1527 72
Fragments arranged by size:
1527 636 72
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 938 2235
Size: 938 1297
Bpu10I CC'TnA_GC
Cuts at: 0 364 2235
Size: 364 1871
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 1420 2235
Size: 286 1134 815
Fragments arranged by size:
1134 815 286
BsaI GGTCTCn'nnnn_
Cuts at: 0 1282 2045 2235
Size: 1282 763 190
Fragments arranged by size:
1282 763 190
BsaAI yAC'GTr
Cuts at: 0 1728 2235
Size: 1728 507
BsaHI Gr'CG_yC
Cuts at: 0 218 487 973 1052 1085 1148 1460 1646
Size: 218 269 486 79 33 63 312 186
Cuts at: 1646 1829 2177 2235
Size: 183 348 58
Fragments arranged by size:
486 348 312 269 218 186 183 79
63 58 33
BsaWI w'CCGG_w
Cuts at: 0 855 2235
Size: 855 1380
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 936 966 2098 2128 2235
Size: 936 30 1132 30 107
Fragments arranged by size:
1132 936 107 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1321 1342 1378 2014 2092 2146 2235
Size: 1321 21 36 636 78 54 89
Fragments arranged by size:
1321 636 89 78 54 36 21
BseYI C'CCAG_C
Cuts at: 0 697 978 1080 1455 1523 2235
Size: 697 281 102 375 68 712
Fragments arranged by size:
712 697 375 281 102 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1519 1642 2035 2235
Size: 1519 123 393 200
Fragments arranged by size:
1519 393 200 123
BsiEI CG_ry'CG
Cuts at: 0 1678 2163 2235
Size: 1678 485 72
Fragments arranged by size:
1678 485 72
BsiHKAI G_wGCw'C
Cuts at: 0 595 693 1071 1436 1760 2235
Size: 595 98 378 365 324 475
Fragments arranged by size:
595 475 378 365 324 98
BsiWI C'GTAC_G
Cuts at: 0 478 2235
Size: 478 1757
BsmI GAATG_Cn'
Cuts at: 0 1277 2235
Size: 1277 958
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1288 2235
Size: 1288 947
Bsp1286I G_dGCh'C
Cuts at: 0 18 409 595 617 693 1028 1071 1130
Size: 18 391 186 22 76 335 43 59
Cuts at: 1130 1436 1531 1538 1557 1760 1820 2149 2235
Size: 306 95 7 19 203 60 329 86
Fragments arranged by size:
391 335 329 306 203 186 95 86
76 60 59 43 22 19 18 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 362 452 2075 2235
Size: 362 90 1623 160
Fragments arranged by size:
1623 362 160 90
BsrBI CCG'CTC
Cuts at: 0 866 2235
Size: 866 1369
BsrDI GCAATG_nn'
Cuts at: 0 623 817 2235
Size: 623 194 1418
Fragments arranged by size:
1418 623 194
BsrFI r'CCGG_y
Cuts at: 0 470 660 855 969 2235
Size: 470 190 195 114 1266
Fragments arranged by size:
1266 470 195 190 114
BssHII G'CGCG_C
Cuts at: 0 368 512 750 1519 2235
Size: 368 144 238 769 716
Fragments arranged by size:
769 716 368 238 144
BstAPI GCAn_nnn'nTGC
Cuts at: 0 430 657 2235
Size: 430 227 1578
Fragments arranged by size:
1578 430 227
BstYI r'GATC_y
Cuts at: 0 824 1343 2235
Size: 824 519 892
Fragments arranged by size:
892 824 519
Bsu36I CC'TnA_GG
Cuts at: 0 1892 2235
Size: 1892 343
BtgI C'CryG_G
Cuts at: 0 266 343 1665 2235
Size: 266 77 1322 570
Fragments arranged by size:
1322 570 266 77
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 765 1449 1748 1905 2178 2235
Size: 765 684 299 157 273 57
Fragments arranged by size:
765 684 299 273 157 57
BtsI GCAGTG_nn'
Cuts at: 0 549 745 774 1625 1630 1790 2235
Size: 549 196 29 851 5 160 445
Fragments arranged by size:
851 549 445 196 160 29 5
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1088 1123 2235
Size: 1088 35 1112
Fragments arranged by size:
1112 1088 35
DraIII CAC_nnn'GTG
Cuts at: 0 430 2235
Size: 430 1805
EaeI y'GGCC_r
Cuts at: 0 47 284 789 967 1265 1675 1808 2235
Size: 47 237 505 178 298 410 133 427
Fragments arranged by size:
505 427 410 298 237 178 133 47
EagI C'GGCC_G
Cuts at: 0 1675 2235
Size: 1675 560
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 904 1375 1495 1565 2235
Size: 904 471 120 70 670
Fragments arranged by size:
904 670 471 120 70
EcoICRI GAG'CTC
Cuts at: 0 1434 1758 2235
Size: 1434 324 477
Fragments arranged by size:
1434 477 324
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 805 938 2235
Size: 805 133 1297
Fragments arranged by size:
1297 805 133
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1267 2235
Size: 1267 968
EcoO109I rG'GnC_Cy
Cuts at: 0 15 206 1127 1173 1386 1527 1528 1896
Size: 15 191 921 46 213 141 1 368
Cuts at: 1896 2025 2064 2235
Size: 129 39 171
Fragments arranged by size:
921 368 213 191 171 141 129 46
39 15 1
FspI TGC'GCA
Cuts at: 0 262 358 457 2235
Size: 262 96 99 1778
Fragments arranged by size:
1778 262 99 96
FspAI rTGC'GCAy
Cuts at: 0 262 2235
Size: 262 1973
HaeII r_GCGC'y
Cuts at: 0 37 490 976 1088 1151 1374 1708 1832
Size: 37 453 486 112 63 223 334 124
Cuts at: 1832 2180 2235
Size: 348 55
Fragments arranged by size:
486 453 348 334 223 124 112 63
55 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 153 185 1177 1209 1747 1779 2098 2130
Size: 153 32 992 32 538 32 319 32
Cuts at: 2130 2235
Size: 105
Fragments arranged by size:
992 538 319 153 105 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1650 2190 2235
Size: 1650 540 45
Fragments arranged by size:
1650 540 45
KasI G'GCGC_C
Cuts at: 0 486 972 1084 1147 1828 2176 2235
Size: 486 486 112 63 681 348 59
Fragments arranged by size:
681 486 486 348 112 63 59
KpnI G_GTAC'C
Cuts at: 0 1685 2235
Size: 1685 550
MluI A'CGCG_T
Cuts at: 0 1104 1412 2235
Size: 1104 308 823
Fragments arranged by size:
1104 823 308
MscI TGG'CCA
Cuts at: 0 1267 1810 2235
Size: 1267 543 425
Fragments arranged by size:
1267 543 425
MslI CAynn'nnrTG
Cuts at: 0 598 710 2235
Size: 598 112 1525
Fragments arranged by size:
1525 598 112
MspA1I CmG'CkG
Cuts at: 0 378 1503 1512 1603 1667 2235
Size: 378 1125 9 91 64 568
Fragments arranged by size:
1125 568 378 91 64 9
NaeI GCC'GGC
Cuts at: 0 971 2235
Size: 971 1264
NarI GG'CG_CC
Cuts at: 0 487 973 1085 1148 1829 2177 2235
Size: 487 486 112 63 681 348 58
Fragments arranged by size:
681 487 486 348 112 63 58
NgoMIV G'CCGG_C
Cuts at: 0 969 2235
Size: 969 1266
PasI CC'CwG_GG
Cuts at: 0 1531 2172 2235
Size: 1531 641 63
Fragments arranged by size:
1531 641 63
PflMI CCAn_nnn'nTGG
Cuts at: 0 1263 1848 2235
Size: 1263 585 387
Fragments arranged by size:
1263 585 387
PmlI CAC'GTG
Cuts at: 0 1728 2235
Size: 1728 507
PpuMI rG'GwC_Cy
Cuts at: 0 1386 2064 2235
Size: 1386 678 171
Fragments arranged by size:
1386 678 171
PspOMI G'GGCC_C
Cuts at: 0 14 1126 1527 1534 2235
Size: 14 1112 401 7 701
Fragments arranged by size:
1112 701 401 14 7
PspXI vC'TCGA_Gb
Cuts at: 0 1954 2235
Size: 1954 281
PstI C_TGCA'G
Cuts at: 0 377 578 653 2072 2235
Size: 377 201 75 1419 163
Fragments arranged by size:
1419 377 201 163 75
PvuI CG_AT'CG
Cuts at: 0 2163 2235
Size: 2163 72
PvuII CAG'CTG
Cuts at: 0 378 1503 2235
Size: 378 1125 732
Fragments arranged by size:
1125 732 378
SacI G_AGCT'C
Cuts at: 0 1436 1760 2235
Size: 1436 324 475
Fragments arranged by size:
1436 475 324
SacII CC_GC'GG
Cuts at: 0 1668 2235
Size: 1668 567
SanDI GG'GwC_CC
Cuts at: 0 1386 2235
Size: 1386 849
SfcI C'TryA_G
Cuts at: 0 373 574 649 2068 2217 2235
Size: 373 201 75 1419 149 18
Fragments arranged by size:
1419 373 201 149 75 18
SfoI GGC'GCC
Cuts at: 0 488 974 1086 1149 1830 2178 2235
Size: 488 486 112 63 681 348 57
Fragments arranged by size:
681 488 486 348 112 63 57
SmaI CCC'GGG
Cuts at: 0 418 598 1566 1862 2235
Size: 418 180 968 296 373
Fragments arranged by size:
968 418 373 296 180
SmlI C'TyrA_G
Cuts at: 0 301 1435 1954 2235
Size: 301 1134 519 281
Fragments arranged by size:
1134 519 301 281
StuI AGG'CCT
Cuts at: 0 580 1262 2235
Size: 580 682 973
Fragments arranged by size:
973 682 580
StyI C'CwwG_G
Cuts at: 0 1040 1202 1257 1268 2117 2235
Size: 1040 162 55 11 849 118
Fragments arranged by size:
1040 849 162 118 55 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1306 2235
Size: 1306 929
TatI w'GTAC_w
Cuts at: 0 2153 2235
Size: 2153 82
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 977 1009 2235
Size: 977 32 1226
Fragments arranged by size:
1226 977 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 962 2206 2235
Size: 962 1244 29
Fragments arranged by size:
1244 962 29
XhoI C'TCGA_G
Cuts at: 0 1954 2235
Size: 1954 281
XmaI C'CCGG_G
Cuts at: 0 416 596 1564 1860 2235
Size: 416 180 968 296 375
Fragments arranged by size:
968 416 375 296 180
ZraI GAC'GTC
Cuts at: 0 219 2235
Size: 219 2016
Enzymes that do cut:
AatII AccI Acc65I AcuI AfeI AflIII AgeI AhdI
AleI AlfI ApaI ApaLI AseI AvaI BanI BanII
BbeI BbsI BcgI BglI BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BstAPI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII EaeI
EagI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI FspAI
HaeII Hin4I HincII KasI KpnI MluI MscI MslI
MspA1I NaeI NarI NgoMIV PasI PflMI PmlI PpuMI
PspOMI PspXI PstI PvuI PvuII SacI SacII SanDI
SfcI SfoI SmaI SmlI StuI StyI TaqII TatI
TstI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AarI AclI AflII AloI AlwNI ApoI AscI AsiSI
AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BglII
BmtI BsaBI BspEI BspHI BsrGI BssSI BstBI BstEII
BstXI BstZ17I ClaI DraI DrdI EarI EcoRI EcoRV
FalI FseI HindIII HpaI MfeI MmeI NcoI NdeI
NheI NotI NruI NsiI NspI PacI PciI PfoI
PmeI PpiI PshAI PsiI PsrI RsrII SalI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI Tth111I XbaI XmnI