Restriction map for
pF1KA1472
(Linear) (Six-base) MAPSORT of: KIAA1472.seq Check: 9608 from: 1 to: 1989
KIAA1472.orf
With 182 enzymes: SgfI *
November 13, 2006 11:20 ..
AccI GT'mk_AC
Cuts at: 0 493 1989
Size: 493 1496
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 874 1102 1989
Size: 874 228 887
Fragments arranged by size:
887 874 228
AflII C'TTAA_G
Cuts at: 0 904 1989
Size: 904 1085
AflIII A'CryG_T
Cuts at: 0 749 1989
Size: 749 1240
AhdI GACnn_n'nnGTC
Cuts at: 0 612 1989
Size: 612 1377
AlwNI CAG_nnn'CTG
Cuts at: 0 133 221 286 1357 1934 1989
Size: 133 88 65 1071 577 55
Fragments arranged by size:
1071 577 133 88 65 55
ApaI G_GGCC'C
Cuts at: 0 8 1989
Size: 8 1981
ApoI r'AATT_y
Cuts at: 0 71 1989
Size: 71 1918
AvaI C'yCGr_G
Cuts at: 0 450 1545 1989
Size: 450 1095 444
Fragments arranged by size:
1095 450 444
BamHI G'GATC_C
Cuts at: 0 1899 1989
Size: 1899 90
BanI G'GyrC_C
Cuts at: 0 1476 1989
Size: 1476 513
BanII G_rGCy'C
Cuts at: 0 8 643 1505 1989
Size: 8 635 862 484
Fragments arranged by size:
862 635 484 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 277 506 1989
Size: 277 229 1483
Fragments arranged by size:
1483 277 229
BglI GCCn_nnn'nGGC
Cuts at: 0 302 973 1989
Size: 302 671 1016
Fragments arranged by size:
1016 671 302
BlpI GC'TnA_GC
Cuts at: 0 192 637 1989
Size: 192 445 1352
Fragments arranged by size:
1352 445 192
Bme1580I G_kGCm'C
Cuts at: 0 8 1481 1989
Size: 8 1473 508
Fragments arranged by size:
1473 508 8
BmgBI CAC'GTC
Cuts at: 0 1473 1989
Size: 1473 516
BmrI ACTGGGnnnn_n'
Cuts at: 0 653 963 1989
Size: 653 310 1026
Fragments arranged by size:
1026 653 310
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1623 1989
Size: 1623 366
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1126 1387 1423 1513 1827 1989
Size: 1126 261 36 90 314 162
Fragments arranged by size:
1126 314 261 162 90 36
Bpu10I CC'TnA_GC
Cuts at: 0 1927 1989
Size: 1927 62
BsaI GGTCTCn'nnnn_
Cuts at: 0 1666 1989
Size: 1666 323
BsaHI Gr'CG_yC
Cuts at: 0 1555 1790 1989
Size: 1555 235 199
Fragments arranged by size:
1555 235 199
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 682 712 892 922 1989
Size: 682 30 180 30 1067
Fragments arranged by size:
1067 682 180 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 438 958 961 1447 1989
Size: 438 520 3 486 542
Fragments arranged by size:
542 520 486 438 3
BseYI C'CCAG_C
Cuts at: 0 126 175 911 1488 1989
Size: 126 49 736 577 501
Fragments arranged by size:
736 577 501 126 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 645 1989
Size: 58 587 1344
Fragments arranged by size:
1344 587 58
BsiHKAI G_wGCw'C
Cuts at: 0 32 1505 1989
Size: 32 1473 484
Fragments arranged by size:
1473 484 32
BsmI GAATG_Cn'
Cuts at: 0 374 1061 1377 1873 1953 1989
Size: 374 687 316 496 80 36
Fragments arranged by size:
687 496 374 316 80 36
Bsp1286I G_dGCh'C
Cuts at: 0 8 32 643 1481 1505 1989
Size: 8 24 611 838 24 484
Fragments arranged by size:
838 611 484 24 24 8
BspHI T'CATG_A
Cuts at: 0 45 728 1718 1989
Size: 45 683 990 271
Fragments arranged by size:
990 683 271 45
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1245 1989
Size: 1245 744
BsrBI CCG'CTC
Cuts at: 0 475 1989
Size: 475 1514
BstAPI GCAn_nnn'nTGC
Cuts at: 0 36 1989
Size: 36 1953
BstEII G'GTnAC_C
Cuts at: 0 1360 1989
Size: 1360 629
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1422 1989
Size: 1422 567
BstYI r'GATC_y
Cuts at: 0 1827 1899 1989
Size: 1827 72 90
Fragments arranged by size:
1827 90 72
Bsu36I CC'TnA_GG
Cuts at: 0 479 1989
Size: 479 1510
BtgI C'CryG_G
Cuts at: 0 595 1667 1850 1859 1989
Size: 595 1072 183 9 130
Fragments arranged by size:
1072 595 183 130 9
BtsI GCAGTG_nn'
Cuts at: 0 808 1446 1989
Size: 808 638 543
Fragments arranged by size:
808 638 543
DraIII CAC_nnn'GTG
Cuts at: 0 36 120 1141 1989
Size: 36 84 1021 848
Fragments arranged by size:
1021 848 84 36
EarI CTCTTCn'nnn_
Cuts at: 0 149 1189 1244 1745 1989
Size: 149 1040 55 501 244
Fragments arranged by size:
1040 501 244 149 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 561 1951 1989
Size: 561 1390 38
Fragments arranged by size:
1390 561 38
EcoICRI GAG'CTC
Cuts at: 0 1503 1989
Size: 1503 486
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 874 1102 1126 1387 1423 1513 1827 1989
Size: 874 228 24 261 36 90 314 162
Fragments arranged by size:
874 314 261 228 162 90 36 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1926 1989
Size: 1926 63
EcoO109I rG'GnC_Cy
Cuts at: 0 4 5 535 606 1533 1919 1989
Size: 4 1 530 71 927 386 70
Fragments arranged by size:
927 530 386 71 70 4 1
EcoRI G'AATT_C
Cuts at: 0 71 1989
Size: 71 1918
HaeII r_GCGC'y
Cuts at: 0 192 870 1989
Size: 192 678 1119
Fragments arranged by size:
1119 678 192
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 430 462 598 630 892 924 1225 1257
Size: 430 32 136 32 262 32 301 32
Cuts at: 1257 1989
Size: 732
Fragments arranged by size:
732 430 301 262 136 32 32 32
32
HincII GTy'rAC
Cuts at: 0 494 1989
Size: 494 1495
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1739 1989
Size: 1739 250
MslI CAynn'nnrTG
Cuts at: 0 1129 1420 1989
Size: 1129 291 569
Fragments arranged by size:
1129 569 291
MspA1I CmG'CkG
Cuts at: 0 597 636 915 1989
Size: 597 39 279 1074
Fragments arranged by size:
1074 597 279 39
NdeI CA'TA_TG
Cuts at: 0 98 488 1989
Size: 98 390 1501
Fragments arranged by size:
1501 390 98
NspI r_CATG'y
Cuts at: 0 753 927 1989
Size: 753 174 1062
Fragments arranged by size:
1062 753 174
PasI CC'CwG_GG
Cuts at: 0 1387 1989
Size: 1387 602
PciI A'CATG_T
Cuts at: 0 749 1989
Size: 749 1240
PfoI T'CCnGG_A
Cuts at: 0 1400 1529 1989
Size: 1400 129 460
Fragments arranged by size:
1400 460 129
PpuMI rG'GwC_Cy
Cuts at: 0 535 606 1533 1989
Size: 535 71 927 456
Fragments arranged by size:
927 535 456 71
PshAI GACnn'nnGTC
Cuts at: 0 1538 1989
Size: 1538 451
PspOMI G'GGCC_C
Cuts at: 0 4 1989
Size: 4 1985
PspXI vC'TCGA_Gb
Cuts at: 0 450 1545 1989
Size: 450 1095 444
Fragments arranged by size:
1095 450 444
PstI C_TGCA'G
Cuts at: 0 815 1523 1989
Size: 815 708 466
Fragments arranged by size:
815 708 466
PvuII CAG'CTG
Cuts at: 0 636 915 1989
Size: 636 279 1074
Fragments arranged by size:
1074 636 279
SacI G_AGCT'C
Cuts at: 0 1505 1989
Size: 1505 484
SacII CC_GC'GG
Cuts at: 0 598 1989
Size: 598 1391
SalI G'TCGA_C
Cuts at: 0 492 1989
Size: 492 1497
SapI GCTCTTCn'nnn_
Cuts at: 0 1189 1244 1989
Size: 1189 55 745
Fragments arranged by size:
1189 745 55
ScaI AGT'ACT
Cuts at: 0 1804 1881 1989
Size: 1804 77 108
Fragments arranged by size:
1804 108 77
SexAI A'CCwGG_T
Cuts at: 0 423 1989
Size: 423 1566
SfcI C'TryA_G
Cuts at: 0 717 811 1482 1519 1989
Size: 717 94 671 37 470
Fragments arranged by size:
717 671 470 94 37
SmlI C'TyrA_G
Cuts at: 0 450 904 1545 1989
Size: 450 454 641 444
Fragments arranged by size:
641 454 450 444
SphI G_CATG'C
Cuts at: 0 927 1989
Size: 927 1062
SspI AAT'ATT
Cuts at: 0 1072 1989
Size: 1072 917
StuI AGG'CCT
Cuts at: 0 413 1989
Size: 413 1576
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1483 1989
Size: 1483 506
TatI w'GTAC_w
Cuts at: 0 1802 1879 1989
Size: 1802 77 110
Fragments arranged by size:
1802 110 77
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 106 138 225 257 1147 1179 1989
Size: 106 32 87 32 890 32 810
Fragments arranged by size:
890 810 106 87 32 32 32
XhoI C'TCGA_G
Cuts at: 0 450 1545 1989
Size: 450 1095 444
Fragments arranged by size:
1095 450 444
Enzymes that do cut:
AccI AcuI AflII AflIII AhdI AlwNI ApaI ApoI
AvaI BamHI BanI BanII BbsI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BsaI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI
DraIII EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
HaeII Hin4I HincII MmeI MslI MspA1I NdeI NspI
PasI PciI PfoI PpuMI PshAI PspOMI PspXI PstI
PvuII SacI SacII SalI SapI ScaI SexAI SfcI
SmlI SphI SspI StuI TaqII TatI TstI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AleI AlfI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BbeI
BbvCI BcgI BciVI BclI BfrBI BglII BmtI BpuEI
BsaAI BsaBI BsaWI BsiEI BsiWI BsmBI BspEI BsrDI
BsrFI BsrGI BssHII BssSI BstBI BstZ17I BtgZI ClaI
CspCI DraI DrdI EaeI EagI EcoRV FalI FseI
FspI FspAI HindIII HpaI KasI KpnI MfeI MluI
MscI NaeI NarI NcoI NgoMIV NheI NotI NruI
NsiI PacI PflMI PmeI PmlI PpiI PsiI PsrI
PvuI RsrII SanDI SbfI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI StyI SwaI Tth111I XbaI
XcmI XmaI XmnI ZraI