Restriction map for
pF1KSDA1473
(Linear) (Six-base) MAPSORT of: KIAA1473.seq Check: 6270 from: 1 to: 1593
KIAA1473.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
ApaLI G'TGCA_C
Cuts at: 0 277 1593
Size: 277 1316
ApoI r'AATT_y
Cuts at: 0 231 370 378 482 566 734 919 986
Size: 231 139 8 104 84 168 185 67
Cuts at: 986 1070 1087 1154 1593
Size: 84 17 67 439
Fragments arranged by size:
439 231 185 168 139 104 84 84
67 67 17 8
AvaI C'yCGr_G
Cuts at: 0 149 1593
Size: 149 1444
BbsI GAAGACnn'nnnn_
Cuts at: 0 18 1593
Size: 18 1575
BclI T'GATC_A
Cuts at: 0 59 1593
Size: 59 1534
Bme1580I G_kGCm'C
Cuts at: 0 281 1593
Size: 281 1312
BmrI ACTGGGnnnn_n'
Cuts at: 0 121 1593
Size: 121 1472
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1525 1593
Size: 1525 68
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 764 848 932 1016 1100 1184 1268
Size: 91 673 84 84 84 84 84 84
Cuts at: 1268 1352 1436 1520 1593
Size: 84 84 84 73
Fragments arranged by size:
673 91 84 84 84 84 84 84
84 84 84 73
BsaI GGTCTCn'nnnn_
Cuts at: 0 146 532 1593
Size: 146 386 1061
Fragments arranged by size:
1061 386 146
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 697 1455 1593
Size: 697 758 138
Fragments arranged by size:
758 697 138
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 791 1593
Size: 791 802
BsiHKAI G_wGCw'C
Cuts at: 0 281 1593
Size: 281 1312
Bsp1286I G_dGCh'C
Cuts at: 0 281 1593
Size: 281 1312
BsrDI GCAATG_nn'
Cuts at: 0 1547 1593
Size: 1547 46
BsrFI r'CCGG_y
Cuts at: 0 620 1124 1593
Size: 620 504 469
Fragments arranged by size:
620 504 469
DraI TTT'AAA
Cuts at: 0 1038 1593
Size: 1038 555
EaeI y'GGCC_r
Cuts at: 0 160 1593
Size: 160 1433
EarI CTCTTCn'nnn_
Cuts at: 0 94 593 1593
Size: 94 499 1000
Fragments arranged by size:
1000 499 94
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 764 848 932 1016 1100 1184 1268
Size: 91 673 84 84 84 84 84 84
Cuts at: 1268 1352 1436 1520 1593
Size: 84 84 84 73
Fragments arranged by size:
673 91 84 84 84 84 84 84
84 84 84 73
EcoRI G'AATT_C
Cuts at: 0 482 566 734 986 1070 1154 1593
Size: 482 84 168 252 84 84 439
Fragments arranged by size:
482 439 252 168 84 84 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 51 83 1593
Size: 51 32 1510
Fragments arranged by size:
1510 51 32
HindIII A'AGCT_T
Cuts at: 0 695 863 1031 1199 1283 1367 1593
Size: 695 168 168 168 84 84 226
Fragments arranged by size:
695 226 168 168 168 84 84
MscI TGG'CCA
Cuts at: 0 162 1593
Size: 162 1431
NspI r_CATG'y
Cuts at: 0 456 1593
Size: 456 1137
SexAI A'CCwGG_T
Cuts at: 0 20 1593
Size: 20 1573
SfcI C'TryA_G
Cuts at: 0 12 1593
Size: 12 1581
SphI G_CATG'C
Cuts at: 0 456 1593
Size: 456 1137
SspI AAT'ATT
Cuts at: 0 335 1593
Size: 335 1258
StuI AGG'CCT
Cuts at: 0 949 1593
Size: 949 644
StyI C'CwwG_G
Cuts at: 0 88 1593
Size: 88 1505
XmnI GAAnn'nnTTC
Cuts at: 0 418 990 1593
Size: 418 572 603
Fragments arranged by size:
603 572 418
Enzymes that do cut:
ApaLI ApoI AvaI BbsI BclI Bme1580I BmrI BplI
BpmI BsaI BseRI BsgI BsiHKAI Bsp1286I BsrDI BsrFI
DraI EaeI EarI Eco57MI EcoRI Hin4I HindIII MscI
NspI SexAI SfcI SphI SspI StuI StyI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
AscI AseI AsiSI AvrII BaeI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BfrBI BglI BglII BlpI
BmgBI BmtI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspI FspAI HaeII HincII HpaI
KasI KpnI MfeI MluI MmeI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SrfI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI ZraI