Restriction map for
pF1KA1478
(Linear) (Six-base) MAPSORT of: KIAA1478.seq Check: 6027 from: 1 to: 1896
KIAA1478.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AccI GT'mk_AC
Cuts at: 0 623 1896
Size: 623 1273
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 550 961 1896
Size: 235 315 411 935
Fragments arranged by size:
935 411 315 235
AflIII A'CryG_T
Cuts at: 0 1659 1896
Size: 1659 237
AhdI GACnn_n'nnGTC
Cuts at: 0 1062 1896
Size: 1062 834
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 757 789 1896
Size: 757 32 1107
Fragments arranged by size:
1107 757 32
AlwNI CAG_nnn'CTG
Cuts at: 0 187 664 1896
Size: 187 477 1232
Fragments arranged by size:
1232 477 187
ApaI G_GGCC'C
Cuts at: 0 686 1896
Size: 686 1210
ApoI r'AATT_y
Cuts at: 0 922 1195 1896
Size: 922 273 701
Fragments arranged by size:
922 701 273
AvrII C'CTAG_G
Cuts at: 0 1854 1896
Size: 1854 42
BanII G_rGCy'C
Cuts at: 0 686 1896
Size: 686 1210
BbsI GAAGACnn'nnnn_
Cuts at: 0 523 1358 1896
Size: 523 835 538
Fragments arranged by size:
835 538 523
BbvCI CC'TCA_GC
Cuts at: 0 266 1225 1896
Size: 266 959 671
Fragments arranged by size:
959 671 266
BglI GCCn_nnn'nGGC
Cuts at: 0 1486 1896
Size: 1486 410
Bme1580I G_kGCm'C
Cuts at: 0 686 829 1529 1896
Size: 686 143 700 367
Fragments arranged by size:
700 686 367 143
BmrI ACTGGGnnnn_n'
Cuts at: 0 1534 1732 1896
Size: 1534 198 164
Fragments arranged by size:
1534 198 164
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 334 1466 1896
Size: 334 1132 430
Fragments arranged by size:
1132 430 334
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 817 1627 1896
Size: 817 810 269
Fragments arranged by size:
817 810 269
Bpu10I CC'TnA_GC
Cuts at: 0 266 1225 1896
Size: 266 959 671
Fragments arranged by size:
959 671 266
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1741 1876 1896
Size: 1741 135 20
Fragments arranged by size:
1741 135 20
BsaI GGTCTCn'nnnn_
Cuts at: 0 969 1896
Size: 969 927
BsaAI yAC'GTr
Cuts at: 0 226 1896
Size: 226 1670
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 757 787 1896
Size: 757 30 1109
Fragments arranged by size:
1109 757 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 370 1213 1896
Size: 370 843 683
Fragments arranged by size:
843 683 370
BseYI C'CCAG_C
Cuts at: 0 150 412 1896
Size: 150 262 1484
Fragments arranged by size:
1484 262 150
BsiHKAI G_wGCw'C
Cuts at: 0 201 1896
Size: 201 1695
BsmI GAATG_Cn'
Cuts at: 0 341 1004 1052 1896
Size: 341 663 48 844
Fragments arranged by size:
844 663 341 48
Bsp1286I G_dGCh'C
Cuts at: 0 201 686 829 1529 1896
Size: 201 485 143 700 367
Fragments arranged by size:
700 485 367 201 143
BspHI T'CATG_A
Cuts at: 0 1427 1625 1896
Size: 1427 198 271
Fragments arranged by size:
1427 271 198
BsrDI GCAATG_nn'
Cuts at: 0 414 1896
Size: 414 1482
BstAPI GCAn_nnn'nTGC
Cuts at: 0 45 1896
Size: 45 1851
BstBI TT'CG_AA
Cuts at: 0 1278 1896
Size: 1278 618
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1384 1896
Size: 1384 512
BstYI r'GATC_y
Cuts at: 0 165 1154 1896
Size: 165 989 742
Fragments arranged by size:
989 742 165
EarI CTCTTCn'nnn_
Cuts at: 0 510 529 1301 1896
Size: 510 19 772 595
Fragments arranged by size:
772 595 510 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 550 817 961 1627 1896
Size: 235 315 267 144 666 269
Fragments arranged by size:
666 315 269 267 235 144
EcoO109I rG'GnC_Cy
Cuts at: 0 1726 1896
Size: 1726 170
EcoRV GAT'ATC
Cuts at: 0 465 1242 1896
Size: 465 777 654
Fragments arranged by size:
777 654 465
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1291 1312 1896
Size: 1291 21 584
Fragments arranged by size:
1291 584 21
HaeII r_GCGC'y
Cuts at: 0 552 1590 1896
Size: 552 1038 306
Fragments arranged by size:
1038 552 306
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 349 381 487 519 1048 1080 1896
Size: 349 32 106 32 529 32 816
Fragments arranged by size:
816 529 349 106 32 32 32
HincII GTy'rAC
Cuts at: 0 1570 1896
Size: 1570 326
MslI CAynn'nnrTG
Cuts at: 0 1096 1630 1896
Size: 1096 534 266
Fragments arranged by size:
1096 534 266
MspA1I CmG'CkG
Cuts at: 0 303 795 989 1896
Size: 303 492 194 907
Fragments arranged by size:
907 492 303 194
NspI r_CATG'y
Cuts at: 0 223 1663 1896
Size: 223 1440 233
Fragments arranged by size:
1440 233 223
PciI A'CATG_T
Cuts at: 0 1659 1896
Size: 1659 237
PflMI CCAn_nnn'nTGG
Cuts at: 0 150 1896
Size: 150 1746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 757 789 1779 1811 1896
Size: 757 32 990 32 85
Fragments arranged by size:
990 757 85 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1726 1896
Size: 1726 170
PshAI GACnn'nnGTC
Cuts at: 0 989 1896
Size: 989 907
PspOMI G'GGCC_C
Cuts at: 0 682 1896
Size: 682 1214
PstI C_TGCA'G
Cuts at: 0 621 1896
Size: 621 1275
PvuII CAG'CTG
Cuts at: 0 303 795 1896
Size: 303 492 1101
Fragments arranged by size:
1101 492 303
SfcI C'TryA_G
Cuts at: 0 617 656 1254 1896
Size: 617 39 598 642
Fragments arranged by size:
642 617 598 39
SmlI C'TyrA_G
Cuts at: 0 1756 1891 1896
Size: 1756 135 5
Fragments arranged by size:
1756 135 5
SphI G_CATG'C
Cuts at: 0 223 1896
Size: 223 1673
StuI AGG'CCT
Cuts at: 0 1145 1896
Size: 1145 751
StyI C'CwwG_G
Cuts at: 0 760 1574 1854 1896
Size: 760 814 280 42
Fragments arranged by size:
814 760 280 42
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1775 1807 1896
Size: 1775 32 89
Fragments arranged by size:
1775 89 32
XmnI GAAnn'nnTTC
Cuts at: 0 520 1295 1896
Size: 520 775 601
Fragments arranged by size:
775 601 520
Enzymes that do cut:
AccI AcuI AflIII AhdI AloI AlwNI ApaI ApoI
AvrII BanII BbsI BbvCI BglI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaXI BseRI BseYI
BsiHKAI BsmI Bsp1286I BspHI BsrDI BstAPI BstBI BstXI
BstYI EarI Eco57MI EcoO109I EcoRV FalI HaeII Hin4I
HincII MslI MspA1I NspI PciI PflMI PpiI PpuMI
PshAI PspOMI PstI PvuII SfcI SmlI SphI StuI
StyI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AleI
AlfI ApaLI AscI AseI AsiSI AvaI BaeI BamHI
BanI BbeI BcgI BciVI BclI BfrBI BglII BlpI
BmgBI BmtI BsaBI BsaHI BsaWI BsgI BsiEI BsiWI
BsmBI BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstEII BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI
EcoRI FseI FspI FspAI HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PfoI
PmeI PmlI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
SwaI TaqII TatI Tth111I XbaI XcmI XhoI XmaI
ZraI