Restriction map for
pF1KA1480
(Linear) (Six-base) MAPSORT of: KIAA1480.seq Check: 964 from: 1 to: 2424
KIAA1480.orf
With 182 enzymes: SgfI *
September 4, 2008 14:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1914 2424
Size: 1914 510
AccI GT'mk_AC
Cuts at: 0 491 1252 2424
Size: 491 761 1172
Fragments arranged by size:
1172 761 491
AclI AA'CG_TT
Cuts at: 0 246 2045 2424
Size: 246 1799 379
Fragments arranged by size:
1799 379 246
AflIII A'CryG_T
Cuts at: 0 1787 2424
Size: 1787 637
AleI CACnn'nnGTG
Cuts at: 0 337 1738 1816 2201 2424
Size: 337 1401 78 385 223
Fragments arranged by size:
1401 385 337 223 78
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 962 2424
Size: 962 1462
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1600 1632 2424
Size: 1600 32 792
Fragments arranged by size:
1600 792 32
ApaI G_GGCC'C
Cuts at: 0 2136 2188 2424
Size: 2136 52 236
Fragments arranged by size:
2136 236 52
AvaI C'yCGr_G
Cuts at: 0 161 738 1673 2417 2424
Size: 161 577 935 744 7
Fragments arranged by size:
935 744 577 161 7
BamHI G'GATC_C
Cuts at: 0 1584 2424
Size: 1584 840
BanI G'GyrC_C
Cuts at: 0 157 213 1761 2087 2424
Size: 157 56 1548 326 337
Fragments arranged by size:
1548 337 326 157 56
BanII G_rGCy'C
Cuts at: 0 40 410 653 1337 2099 2136 2188 2424
Size: 40 370 243 684 762 37 52 236
Fragments arranged by size:
762 684 370 243 236 52 40 37
BbeI G_GCGC'C
Cuts at: 0 2091 2424
Size: 2091 333
BbsI GAAGACnn'nnnn_
Cuts at: 0 422 925 2424
Size: 422 503 1499
Fragments arranged by size:
1499 503 422
BbvCI CC'TCA_GC
Cuts at: 0 97 2117 2424
Size: 97 2020 307
Fragments arranged by size:
2020 307 97
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 531 552 565 586 676 710 1342 1376
Size: 531 21 13 21 90 34 632 34
Cuts at: 1376 2424
Size: 1048
Fragments arranged by size:
1048 632 531 90 34 34 21 21
13
BciVI GTATCCnnnnn_n'
Cuts at: 0 1824 2424
Size: 1824 600
BglI GCCn_nnn'nGGC
Cuts at: 0 2023 2103 2424
Size: 2023 80 321
Fragments arranged by size:
2023 321 80
Bme1580I G_kGCm'C
Cuts at: 0 162 218 319 344 1766 2136 2188 2424
Size: 162 56 101 25 1422 370 52 236
Fragments arranged by size:
1422 370 236 162 101 56 52 25
BmrI ACTGGGnnnn_n'
Cuts at: 0 170 304 1269 1589 2191 2424
Size: 170 134 965 320 602 233
Fragments arranged by size:
965 602 320 233 170 134
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1699 2424
Size: 1699 725
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 641 1899 2138 2424
Size: 641 1258 239 286
Fragments arranged by size:
1258 641 286 239
Bpu10I CC'TnA_GC
Cuts at: 0 34 97 151 2117 2424
Size: 34 63 54 1966 307
Fragments arranged by size:
1966 307 63 54 34
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1096 2424
Size: 1096 1328
BsaI GGTCTCn'nnnn_
Cuts at: 0 704 1114 1228 1276 1670 2424
Size: 704 410 114 48 394 754
Fragments arranged by size:
754 704 410 394 114 48
BsaAI yAC'GTr
Cuts at: 0 993 2424
Size: 993 1431
BsaHI Gr'CG_yC
Cuts at: 0 2088 2424
Size: 2088 336
BsaWI w'CCGG_w
Cuts at: 0 287 1827 2424
Size: 287 1540 597
Fragments arranged by size:
1540 597 287
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2392 2422 2424
Size: 2392 30 2
Fragments arranged by size:
2392 30 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 757 2173 2176 2424
Size: 757 1416 3 248
Fragments arranged by size:
1416 757 248 3
BseYI C'CCAG_C
Cuts at: 0 117 677 839 2424
Size: 117 560 162 1585
Fragments arranged by size:
1585 560 162 117
BsiEI CG_ry'CG
Cuts at: 0 236 2424
Size: 236 2188
BsiHKAI G_wGCw'C
Cuts at: 0 1885 2424
Size: 1885 539
BsmI GAATG_Cn'
Cuts at: 0 1929 2424
Size: 1929 495
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1182 2424
Size: 1182 1242
Bsp1286I G_dGCh'C
Cuts at: 0 40 162 218 319 344 410 653 1337
Size: 40 122 56 101 25 66 243 684
Cuts at: 1337 1766 1885 2099 2136 2188 2424
Size: 429 119 214 37 52 236
Fragments arranged by size:
684 429 243 236 214 122 119 101
66 56 52 40 37 25
BspEI T'CCGG_A
Cuts at: 0 287 1827 2424
Size: 287 1540 597
Fragments arranged by size:
1540 597 287
BspHI T'CATG_A
Cuts at: 0 1418 1550 2294 2424
Size: 1418 132 744 130
Fragments arranged by size:
1418 744 132 130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1787 1914 2424
Size: 1787 127 510
Fragments arranged by size:
1787 510 127
BsrDI GCAATG_nn'
Cuts at: 0 570 2242 2347 2424
Size: 570 1672 105 77
Fragments arranged by size:
1672 570 105 77
BsrFI r'CCGG_y
Cuts at: 0 357 1596 2209 2424
Size: 357 1239 613 215
Fragments arranged by size:
1239 613 357 215
BssSI C'ACGA_G
Cuts at: 0 1982 2197 2424
Size: 1982 215 227
Fragments arranged by size:
1982 227 215
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2349 2424
Size: 2349 75
BstXI CCAn_nnnn'nTGG
Cuts at: 0 879 957 1188 1740 2019 2331 2424
Size: 879 78 231 552 279 312 93
Fragments arranged by size:
879 552 312 279 231 93 78
BstYI r'GATC_y
Cuts at: 0 182 662 1043 1584 2424
Size: 182 480 381 541 840
Fragments arranged by size:
840 541 480 381 182
BstZ17I GTA'TAC
Cuts at: 0 1253 2424
Size: 1253 1171
Bsu36I CC'TnA_GG
Cuts at: 0 1139 1211 2424
Size: 1139 72 1213
Fragments arranged by size:
1213 1139 72
BtgI C'CryG_G
Cuts at: 0 47 498 914 2018 2424
Size: 47 451 416 1104 406
Fragments arranged by size:
1104 451 416 406 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1998 2424
Size: 1998 426
BtsI GCAGTG_nn'
Cuts at: 0 169 368 1404 2237 2424
Size: 169 199 1036 833 187
Fragments arranged by size:
1036 833 199 187 169
DraIII CAC_nnn'GTG
Cuts at: 0 77 2424
Size: 77 2347
DrdI GACnn_nn'nnGTC
Cuts at: 0 1174 2424
Size: 1174 1250
EaeI y'GGCC_r
Cuts at: 0 1891 2424
Size: 1891 533
EarI CTCTTCn'nnn_
Cuts at: 0 1315 2424
Size: 1315 1109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 641 1899 2138 2424
Size: 641 1258 239 286
Fragments arranged by size:
1258 641 286 239
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1484 2424
Size: 1484 940
EcoO109I rG'GnC_Cy
Cuts at: 0 114 190 669 1489 1934 2024 2132 2133
Size: 114 76 479 820 445 90 108 1
Cuts at: 2133 2185 2424
Size: 52 239
Fragments arranged by size:
820 479 445 239 114 108 90 76
52 1
EcoRV GAT'ATC
Cuts at: 0 384 2424
Size: 384 2040
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1761 2424
Size: 1761 663
HaeII r_GCGC'y
Cuts at: 0 97 2091 2274 2424
Size: 97 1994 183 150
Fragments arranged by size:
1994 183 150 97
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 797 829 1135 1167 1223 1255 1338 1370
Size: 797 32 306 32 56 32 83 32
Cuts at: 1370 1569 1601 2424
Size: 199 32 823
Fragments arranged by size:
823 797 306 199 83 56 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 132 1095 2424
Size: 132 963 1329
Fragments arranged by size:
1329 963 132
KasI G'GCGC_C
Cuts at: 0 2087 2424
Size: 2087 337
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 576 2424
Size: 576 1848
MslI CAynn'nnrTG
Cuts at: 0 337 1451 1738 1816 2017 2201 2314 2424
Size: 337 1114 287 78 201 184 113 110
Fragments arranged by size:
1114 337 287 201 184 113 110 78
MspA1I CmG'CkG
Cuts at: 0 337 677 839 2121 2424
Size: 337 340 162 1282 303
Fragments arranged by size:
1282 340 337 303 162
NarI GG'CG_CC
Cuts at: 0 2088 2424
Size: 2088 336
NspI r_CATG'y
Cuts at: 0 1363 1791 2424
Size: 1363 428 633
Fragments arranged by size:
1363 633 428
PasI CC'CwG_GG
Cuts at: 0 1929 2424
Size: 1929 495
PciI A'CATG_T
Cuts at: 0 1787 2424
Size: 1787 637
PflMI CCAn_nnn'nTGG
Cuts at: 0 207 1329 2424
Size: 207 1122 1095
Fragments arranged by size:
1122 1095 207
PfoI T'CCnGG_A
Cuts at: 0 401 461 1936 2424
Size: 401 60 1475 488
Fragments arranged by size:
1475 488 401 60
PmlI CAC'GTG
Cuts at: 0 993 2424
Size: 993 1431
PpuMI rG'GwC_Cy
Cuts at: 0 1934 2424
Size: 1934 490
PspOMI G'GGCC_C
Cuts at: 0 2132 2184 2424
Size: 2132 52 240
Fragments arranged by size:
2132 240 52
PstI C_TGCA'G
Cuts at: 0 2345 2424
Size: 2345 79
PvuI CG_AT'CG
Cuts at: 0 236 2424
Size: 236 2188
PvuII CAG'CTG
Cuts at: 0 337 839 2424
Size: 337 502 1585
Fragments arranged by size:
1585 502 337
ScaI AGT'ACT
Cuts at: 0 618 2424
Size: 618 1806
SfcI C'TryA_G
Cuts at: 0 1911 2341 2424
Size: 1911 430 83
Fragments arranged by size:
1911 430 83
SfoI GGC'GCC
Cuts at: 0 2089 2424
Size: 2089 335
SmaI CCC'GGG
Cuts at: 0 2419 2424
Size: 2419 5
SmlI C'TyrA_G
Cuts at: 0 1111 2424
Size: 1111 1313
SphI G_CATG'C
Cuts at: 0 1363 2424
Size: 1363 1061
SspI AAT'ATT
Cuts at: 0 693 2424
Size: 693 1731
StyI C'CwwG_G
Cuts at: 0 635 950 1628 1708 2424
Size: 635 315 678 80 716
Fragments arranged by size:
716 678 635 315 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 138 669 1317 1852 2206 2424
Size: 138 531 648 535 354 218
Fragments arranged by size:
648 535 531 354 218 138
TatI w'GTAC_w
Cuts at: 0 616 866 2424
Size: 616 250 1558
Fragments arranged by size:
1558 616 250
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 288 320 1786 1818 2392 2424
Size: 288 32 1466 32 574 32
Fragments arranged by size:
1466 574 288 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 183 272 997 2424
Size: 183 89 725 1427
Fragments arranged by size:
1427 725 183 89
XmaI C'CCGG_G
Cuts at: 0 2417 2424
Size: 2417 7
XmnI GAAnn'nnTTC
Cuts at: 0 247 2424
Size: 247 2177
Enzymes that do cut:
AarI AccI AclI AflIII AleI AlfI AloI ApaI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BglI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI
BsrFI BssSI BstAPI BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI DraIII DrdI EaeI EarI Eco57MI EcoNI
EcoO109I EcoRV FalI HaeII Hin4I HincII KasI MmeI
MslI MspA1I NarI NspI PasI PciI PflMI PfoI
PmlI PpuMI PspOMI PstI PvuI PvuII ScaI SfcI
SfoI SmaI SmlI SphI SspI StyI TaqII TatI
TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AcuI AfeI AflII AgeI AhdI AlwNI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BclI
BfrBI BglII BlpI BmgBI BmtI BsaBI BsgI BsiWI
BsrBI BsrGI BssHII BstBI BstEII ClaI CspCI DraI
EagI EciI EcoICRI EcoRI FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI MscI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PmeI PpiI
PshAI PsiI PspXI PsrI RsrII SacI SacII SalI
SanDI SapI SbfI SexAI SfiI SgfI SgrAI SnaBI
SpeI SrfI StuI SwaI Tth111I XbaI XhoI ZraI