Restriction map for
pF1KA1485
(Linear) (Six-base) MAPSORT of: KIAA1485.seq Check: 4614 from: 1 to: 3729
KIAA1485.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 959 1009 2003 3729
Size: 959 50 994 1726
Fragments arranged by size:
1726 994 959 50
AatII G_ACGT'C
Cuts at: 0 1097 3729
Size: 1097 2632
AccI GT'mk_AC
Cuts at: 0 3629 3665 3729
Size: 3629 36 64
Fragments arranged by size:
3629 64 36
Acc65I G'GTAC_C
Cuts at: 0 2255 3729
Size: 2255 1474
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 686 2413 2671 3046 3729
Size: 686 1727 258 375 683
Fragments arranged by size:
1727 686 683 375 258
AflIII A'CryG_T
Cuts at: 0 2819 3729
Size: 2819 910
AgeI A'CCGG_T
Cuts at: 0 2123 3729
Size: 2123 1606
AleI CACnn'nnGTG
Cuts at: 0 1223 2813 3382 3729
Size: 1223 1590 569 347
Fragments arranged by size:
1590 1223 569 347
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 430 1463 1729 3729
Size: 430 1033 266 2000
Fragments arranged by size:
2000 1033 430 266
AlwNI CAG_nnn'CTG
Cuts at: 0 1338 2065 2943 3339 3729
Size: 1338 727 878 396 390
Fragments arranged by size:
1338 878 727 396 390
ApaI G_GGCC'C
Cuts at: 0 668 3729
Size: 668 3061
ApaLI G'TGCA_C
Cuts at: 0 946 1027 1042 1438 1678 2901 3729
Size: 946 81 15 396 240 1223 828
Fragments arranged by size:
1223 946 828 396 240 81 15
ApoI r'AATT_y
Cuts at: 0 420 687 2833 3729
Size: 420 267 2146 896
Fragments arranged by size:
2146 896 420 267
AvaI C'yCGr_G
Cuts at: 0 2294 2593 3729
Size: 2294 299 1136
Fragments arranged by size:
2294 1136 299
AvrII C'CTAG_G
Cuts at: 0 1959 3729
Size: 1959 1770
BamHI G'GATC_C
Cuts at: 0 872 2201 3729
Size: 872 1329 1528
Fragments arranged by size:
1528 1329 872
BanI G'GyrC_C
Cuts at: 0 714 1945 2255 3076 3151 3415 3462 3729
Size: 714 1231 310 821 75 264 47 267
Fragments arranged by size:
1231 821 714 310 267 264 75 47
BanII G_rGCy'C
Cuts at: 0 668 1172 1307 1957 2706 3260 3509 3729
Size: 668 504 135 650 749 554 249 220
Fragments arranged by size:
749 668 650 554 504 249 220 135
BbeI G_GCGC'C
Cuts at: 0 1949 3419 3729
Size: 1949 1470 310
Fragments arranged by size:
1949 1470 310
BbsI GAAGACnn'nnnn_
Cuts at: 0 828 1458 3302 3729
Size: 828 630 1844 427
Fragments arranged by size:
1844 828 630 427
BbvCI CC'TCA_GC
Cuts at: 0 2036 3729
Size: 2036 1693
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1380 1414 3729
Size: 1380 34 2315
Fragments arranged by size:
2315 1380 34
BclI T'GATC_A
Cuts at: 0 1889 3729
Size: 1889 1840
BfrBI ATG'CAT
Cuts at: 0 2277 3729
Size: 2277 1452
BglI GCCn_nnn'nGGC
Cuts at: 0 931 1456 3729
Size: 931 525 2273
Fragments arranged by size:
2273 931 525
BglII A'GATC_T
Cuts at: 0 548 3729
Size: 548 3181
Bme1580I G_kGCm'C
Cuts at: 0 668 904 950 1031 1046 1442 1682 2905
Size: 668 236 46 81 15 396 240 1223
Cuts at: 2905 2991 3079 3729
Size: 86 88 650
Fragments arranged by size:
1223 668 650 396 240 236 88 86
81 46 15
BmgBI CAC'GTC
Cuts at: 0 3603 3729
Size: 3603 126
BmrI ACTGGGnnnn_n'
Cuts at: 0 900 3261 3729
Size: 900 2361 468
Fragments arranged by size:
2361 900 468
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 318 3276 3528 3729
Size: 318 2958 252 201
Fragments arranged by size:
2958 318 252 201
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 986 1558 1645 2042 2245 2411 3316 3729
Size: 986 572 87 397 203 166 905 413
Fragments arranged by size:
986 905 572 413 397 203 166 87
Bpu10I CC'TnA_GC
Cuts at: 0 1006 2036 2117 3729
Size: 1006 1030 81 1612
Fragments arranged by size:
1612 1030 1006 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 162 1006 1576 1751 2755 3729
Size: 162 844 570 175 1004 974
Fragments arranged by size:
1004 974 844 570 175 162
BsaI GGTCTCn'nnnn_
Cuts at: 0 688 1749 2213 2571 3212 3415 3729
Size: 688 1061 464 358 641 203 314
Fragments arranged by size:
1061 688 641 464 358 314 203
BsaAI yAC'GTr
Cuts at: 0 2364 3669 3729
Size: 2364 1305 60
Fragments arranged by size:
2364 1305 60
BsaHI Gr'CG_yC
Cuts at: 0 1094 1946 3416 3431 3729
Size: 1094 852 1470 15 298
Fragments arranged by size:
1470 1094 852 298 15
BsaWI w'CCGG_w
Cuts at: 0 187 312 2123 2204 3729
Size: 187 125 1811 81 1525
Fragments arranged by size:
1811 1525 187 125 81
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1672 1702 3231 3261 3729
Size: 1672 30 1529 30 468
Fragments arranged by size:
1672 1529 468 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 70 277 445 1576 1642 1737 1740 1800
Size: 70 207 168 1131 66 95 3 60
Cuts at: 1800 2041 2613 3073 3268 3514 3517 3709 3729
Size: 241 572 460 195 246 3 192 20
Fragments arranged by size:
1131 572 460 246 241 207 195 192
168 95 70 66 60 20 3 3
BseYI C'CCAG_C
Cuts at: 0 661 1671 1921 2008 2031 3411 3508 3729
Size: 661 1010 250 87 23 1380 97 221
Fragments arranged by size:
1380 1010 661 250 221 97 87 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 599 930 3691 3729
Size: 599 331 2761 38
Fragments arranged by size:
2761 599 331 38
BsiEI CG_ry'CG
Cuts at: 0 3366 3729
Size: 3366 363
BsiHKAI G_wGCw'C
Cuts at: 0 950 1031 1046 1172 1307 1442 1682 2123
Size: 950 81 15 126 135 135 240 441
Cuts at: 2123 2578 2905 3104 3729
Size: 455 327 199 625
Fragments arranged by size:
950 625 455 441 327 240 199 135
135 126 81 15
BsmI GAATG_Cn'
Cuts at: 0 1590 1761 3729
Size: 1590 171 1968
Fragments arranged by size:
1968 1590 171
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1115 3532 3610 3729
Size: 1115 2417 78 119
Fragments arranged by size:
2417 1115 119 78
Bsp1286I G_dGCh'C
Cuts at: 0 668 904 950 1031 1046 1172 1307 1442
Size: 668 236 46 81 15 126 135 135
Cuts at: 1442 1682 1957 2123 2578 2706 2905 2991 3079
Size: 240 275 166 455 128 199 86 88
Cuts at: 3079 3104 3260 3509 3729
Size: 25 156 249 220
Fragments arranged by size:
668 455 275 249 240 236 220 199
166 156 135 135 128 126 88 86
81 46 25 15
BspEI T'CCGG_A
Cuts at: 0 312 2204 3729
Size: 312 1892 1525
Fragments arranged by size:
1892 1525 312
BspHI T'CATG_A
Cuts at: 0 206 2144 2666 2706 3527 3729
Size: 206 1938 522 40 821 202
Fragments arranged by size:
1938 821 522 206 202 40
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 436 959 1009 1766 2003 3480 3729
Size: 436 523 50 757 237 1477 249
Fragments arranged by size:
1477 757 523 436 249 237 50
BsrBI CCG'CTC
Cuts at: 0 2199 3729
Size: 2199 1530
BsrDI GCAATG_nn'
Cuts at: 0 2991 3729
Size: 2991 738
BsrFI r'CCGG_y
Cuts at: 0 2123 3714 3729
Size: 2123 1591 15
Fragments arranged by size:
2123 1591 15
BstAPI GCAn_nnn'nTGC
Cuts at: 0 855 3729
Size: 855 2874
BstEII G'GTnAC_C
Cuts at: 0 1996 3492 3729
Size: 1996 1496 237
Fragments arranged by size:
1996 1496 237
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1921 3115 3729
Size: 1921 1194 614
Fragments arranged by size:
1921 1194 614
BstYI r'GATC_y
Cuts at: 0 548 872 2004 2201 3729
Size: 548 324 1132 197 1528
Fragments arranged by size:
1528 1132 548 324 197
Bsu36I CC'TnA_GG
Cuts at: 0 145 230 868 2561 3251 3729
Size: 145 85 638 1693 690 478
Fragments arranged by size:
1693 690 638 478 145 85
BtgI C'CryG_G
Cuts at: 0 517 1275 1314 1739 1941 3377 3458 3557
Size: 517 758 39 425 202 1436 81 99
Cuts at: 3557 3729
Size: 172
Fragments arranged by size:
1436 758 517 425 202 172 99 81
39
BtsI GCAGTG_nn'
Cuts at: 0 1062 2876 3434 3729
Size: 1062 1814 558 295
Fragments arranged by size:
1814 1062 558 295
ClaI AT'CG_AT
Cuts at: 0 2526 3729
Size: 2526 1203
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3423 3458 3729
Size: 3423 35 271
Fragments arranged by size:
3423 271 35
DraIII CAC_nnn'GTG
Cuts at: 0 1723 2909 3585 3729
Size: 1723 1186 676 144
Fragments arranged by size:
1723 1186 676 144
DrdI GACnn_nn'nnGTC
Cuts at: 0 1147 3729
Size: 1147 2582
EaeI y'GGCC_r
Cuts at: 0 26 1311 3323 3729
Size: 26 1285 2012 406
Fragments arranged by size:
2012 1285 406 26
EarI CTCTTCn'nnn_
Cuts at: 0 61 205 211 254 1553 1592 3729
Size: 61 144 6 43 1299 39 2137
Fragments arranged by size:
2137 1299 144 61 43 39 6
EcoICRI GAG'CTC
Cuts at: 0 1170 1305 3729
Size: 1170 135 2424
Fragments arranged by size:
2424 1170 135
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 686 986 1558 1645 2042 2245 2411 2413
Size: 686 300 572 87 397 203 166 2
Cuts at: 2413 2671 3046 3316 3729
Size: 258 375 270 413
Fragments arranged by size:
686 572 413 397 375 300 270 258
203 166 87 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 674 722 1009 3729
Size: 674 48 287 2720
Fragments arranged by size:
2720 674 287 48
EcoO109I rG'GnC_Cy
Cuts at: 0 665 790 1650 2151 3336 3729
Size: 665 125 860 501 1185 393
Fragments arranged by size:
1185 860 665 501 393 125
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1380 3729
Size: 1380 2349
FspI TGC'GCA
Cuts at: 0 955 1420 3729
Size: 955 465 2309
Fragments arranged by size:
2309 955 465
HaeII r_GCGC'y
Cuts at: 0 1339 1949 3419 3729
Size: 1339 610 1470 310
Fragments arranged by size:
1470 1339 610 310
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 976 1008 1795 1827 2110 2142 2541 2573
Size: 976 32 787 32 283 32 399 32
Cuts at: 2573 3729
Size: 1156
Fragments arranged by size:
1156 976 787 399 283 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 1945 3415 3729
Size: 1945 1470 314
Fragments arranged by size:
1945 1470 314
KpnI G_GTAC'C
Cuts at: 0 2259 3729
Size: 2259 1470
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 313 346 401 3539 3620 3729
Size: 53 260 33 55 3138 81 109
Fragments arranged by size:
3138 260 109 81 55 53 33
MscI TGG'CCA
Cuts at: 0 1313 3729
Size: 1313 2416
MslI CAynn'nnrTG
Cuts at: 0 34 412 601 881 897 1223 1981 2711
Size: 34 378 189 280 16 326 758 730
Cuts at: 2711 2813 3053 3382 3526 3729
Size: 102 240 329 144 203
Fragments arranged by size:
758 730 378 329 326 280 240 203
189 144 102 34 16
MspA1I CmG'CkG
Cuts at: 0 390 1203 1551 1623 1675 1993 2035 2610
Size: 390 813 348 72 52 318 42 575
Cuts at: 2610 2923 2940 2984 3094 3319 3729
Size: 313 17 44 110 225 410
Fragments arranged by size:
813 575 410 390 348 318 313 225
110 72 52 44 42 17
NaeI GCC'GGC
Cuts at: 0 3716 3729
Size: 3716 13
NarI GG'CG_CC
Cuts at: 0 1946 3416 3729
Size: 1946 1470 313
Fragments arranged by size:
1946 1470 313
NcoI C'CATG_G
Cuts at: 0 1314 1941 3458 3729
Size: 1314 627 1517 271
Fragments arranged by size:
1517 1314 627 271
NdeI CA'TA_TG
Cuts at: 0 1265 2780 3729
Size: 1265 1515 949
Fragments arranged by size:
1515 1265 949
NgoMIV G'CCGG_C
Cuts at: 0 3714 3729
Size: 3714 15
NsiI A_TGCA'T
Cuts at: 0 2279 3729
Size: 2279 1450
NspI r_CATG'y
Cuts at: 0 1351 1854 2281 2823 2965 3729
Size: 1351 503 427 542 142 764
Fragments arranged by size:
1351 764 542 503 427 142
PasI CC'CwG_GG
Cuts at: 0 1654 3729
Size: 1654 2075
PciI A'CATG_T
Cuts at: 0 2819 3729
Size: 2819 910
PflMI CCAn_nnn'nTGG
Cuts at: 0 1269 1338 2065 3585 3729
Size: 1269 69 727 1520 144
Fragments arranged by size:
1520 1269 727 144 69
PfoI T'CCnGG_A
Cuts at: 0 1499 3332 3533 3729
Size: 1499 1833 201 196
Fragments arranged by size:
1833 1499 201 196
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1219 1251 3729
Size: 1219 32 2478
Fragments arranged by size:
2478 1219 32
PpuMI rG'GwC_Cy
Cuts at: 0 790 2151 3336 3729
Size: 790 1361 1185 393
Fragments arranged by size:
1361 1185 790 393
PshAI GACnn'nnGTC
Cuts at: 0 519 3729
Size: 519 3210
PspOMI G'GGCC_C
Cuts at: 0 664 3729
Size: 664 3065
PstI C_TGCA'G
Cuts at: 0 860 2883 3398 3729
Size: 860 2023 515 331
Fragments arranged by size:
2023 860 515 331
PvuII CAG'CTG
Cuts at: 0 390 1203 1551 1623 1675 1993 2035 2610
Size: 390 813 348 72 52 318 42 575
Cuts at: 2610 2940 3729
Size: 330 789
Fragments arranged by size:
813 789 575 390 348 330 318 72
52 42
SacI G_AGCT'C
Cuts at: 0 1172 1307 3729
Size: 1172 135 2422
Fragments arranged by size:
2422 1172 135
ScaI AGT'ACT
Cuts at: 0 1087 3729
Size: 1087 2642
SexAI A'CCwGG_T
Cuts at: 0 710 1506 3581 3729
Size: 710 796 2075 148
Fragments arranged by size:
2075 796 710 148
SfcI C'TryA_G
Cuts at: 0 856 1546 2443 2879 3394 3729
Size: 856 690 897 436 515 335
Fragments arranged by size:
897 856 690 515 436 335
SfoI GGC'GCC
Cuts at: 0 1947 3417 3729
Size: 1947 1470 312
Fragments arranged by size:
1947 1470 312
SmaI CCC'GGG
Cuts at: 0 2296 3729
Size: 2296 1433
SmlI C'TyrA_G
Cuts at: 0 177 1021 1591 1766 2770 3729
Size: 177 844 570 175 1004 959
Fragments arranged by size:
1004 959 844 570 177 175
StuI AGG'CCT
Cuts at: 0 150 228 1159 1459 1603 2041 3649 3729
Size: 150 78 931 300 144 438 1608 80
Fragments arranged by size:
1608 931 438 300 150 144 80 78
StyI C'CwwG_G
Cuts at: 0 1160 1314 1460 1907 1941 1959 3458 3729
Size: 1160 154 146 447 34 18 1499 271
Fragments arranged by size:
1499 1160 447 271 154 146 34 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 415 1438 2302 3280 3380 3729
Size: 415 1023 864 978 100 349
Fragments arranged by size:
1023 978 864 415 349 100
TatI w'GTAC_w
Cuts at: 0 157 1085 3729
Size: 157 928 2644
Fragments arranged by size:
2644 928 157
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1672 1704 3729
Size: 1672 32 2025
Fragments arranged by size:
2025 1672 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 600 3687 3729
Size: 600 3087 42
Fragments arranged by size:
3087 600 42
XmaI C'CCGG_G
Cuts at: 0 2294 3729
Size: 2294 1435
XmnI GAAnn'nnTTC
Cuts at: 0 626 2722 3299 3729
Size: 626 2096 577 430
Fragments arranged by size:
2096 626 577 430
ZraI GAC'GTC
Cuts at: 0 1095 3729
Size: 1095 2634
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BcgI BclI BfrBI
BglI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtsI ClaI CspCI DraIII DrdI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII
Hin4I KasI KpnI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PasI PciI
PflMI PfoI PpiI PpuMI PshAI PspOMI PstI PvuII
SacI ScaI SexAI SfcI SfoI SmaI SmlI StuI
StyI TaqII TatI TstI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AfeI AflII AhdI AloI AscI AseI AsiSI
BaeI BciVI BlpI BmtI BsaBI BsiWI BsrGI BssHII
BssSI BstBI BstZ17I BtgZI DraI EagI EciI EcoRI
EcoRV FseI FspAI HincII HindIII HpaI MfeI MluI
NheI NotI NruI PacI PmeI PmlI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI Tth111I XbaI XhoI