Restriction map for
pF1KSDA1490
(Linear) (Six-base) MAPSORT of: KIAA1490.seq Check: 5781 from: 1 to: 2244
KIAA1490.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2203 2244
Size: 2203 41
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 575 579 859 2244
Size: 48 527 4 280 1385
Fragments arranged by size:
1385 527 280 48 4
AflIII A'CryG_T
Cuts at: 0 1883 1930 2244
Size: 1883 47 314
Fragments arranged by size:
1883 314 47
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1504 2244
Size: 1504 740
AlwNI CAG_nnn'CTG
Cuts at: 0 10 2244
Size: 10 2234
ApoI r'AATT_y
Cuts at: 0 559 947 2244
Size: 559 388 1297
Fragments arranged by size:
1297 559 388
BamHI G'GATC_C
Cuts at: 0 1457 1719 2244
Size: 1457 262 525
Fragments arranged by size:
1457 525 262
BanI G'GyrC_C
Cuts at: 0 280 387 2215 2244
Size: 280 107 1828 29
Fragments arranged by size:
1828 280 107 29
BanII G_rGCy'C
Cuts at: 0 321 443 1073 2244
Size: 321 122 630 1171
Fragments arranged by size:
1171 630 321 122
BbvCI CC'TCA_GC
Cuts at: 0 1064 2244
Size: 1064 1180
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 691 725 2244
Size: 691 34 1519
Fragments arranged by size:
1519 691 34
BfrBI ATG'CAT
Cuts at: 0 977 1132 1252 2244
Size: 977 155 120 992
Fragments arranged by size:
992 977 155 120
BglI GCCn_nnn'nGGC
Cuts at: 0 1685 2244
Size: 1685 559
BlpI GC'TnA_GC
Cuts at: 0 584 2244
Size: 584 1660
Bme1580I G_kGCm'C
Cuts at: 0 334 2244
Size: 334 1910
BmrI ACTGGGnnnn_n'
Cuts at: 0 153 2244
Size: 153 2091
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 528 1710 2244
Size: 528 1182 534
Fragments arranged by size:
1182 534 528
Bpu10I CC'TnA_GC
Cuts at: 0 1064 1180 2244
Size: 1064 116 1064
Fragments arranged by size:
1064 1064 116
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 953 2244
Size: 953 1291
BsaAI yAC'GTr
Cuts at: 0 363 2244
Size: 363 1881
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1899 1929 2199 2229 2244
Size: 1899 30 270 30 15
Fragments arranged by size:
1899 270 30 30 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 210 246 391 394 766 1081 1920 2244
Size: 210 36 145 3 372 315 839 324
Fragments arranged by size:
839 372 324 315 210 145 36 3
BseYI C'CCAG_C
Cuts at: 0 151 2244
Size: 151 2093
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 656 2244
Size: 656 1588
BsiHKAI G_wGCw'C
Cuts at: 0 143 199 443 1073 1774 1893 2244
Size: 143 56 244 630 701 119 351
Fragments arranged by size:
701 630 351 244 143 119 56
BsmBI CGTCTCn'nnnn_
Cuts at: 0 80 2244
Size: 80 2164
Bsp1286I G_dGCh'C
Cuts at: 0 143 199 321 334 443 1073 1774 1893
Size: 143 56 122 13 109 630 701 119
Cuts at: 1893 2244
Size: 351
Fragments arranged by size:
701 630 351 143 122 119 109 56
13
BspHI T'CATG_A
Cuts at: 0 914 1962 2244
Size: 914 1048 282
Fragments arranged by size:
1048 914 282
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 678 2203 2244
Size: 678 1525 41
Fragments arranged by size:
1525 678 41
BsrDI GCAATG_nn'
Cuts at: 0 1179 1304 2244
Size: 1179 125 940
Fragments arranged by size:
1179 940 125
BsrFI r'CCGG_y
Cuts at: 0 85 508 2244
Size: 85 423 1736
Fragments arranged by size:
1736 423 85
BssSI C'ACGA_G
Cuts at: 0 300 2244
Size: 300 1944
BstAPI GCAn_nnn'nTGC
Cuts at: 0 42 2244
Size: 42 2202
BstYI r'GATC_y
Cuts at: 0 1457 1719 2244
Size: 1457 262 525
Fragments arranged by size:
1457 525 262
BtgI C'CryG_G
Cuts at: 0 2042 2244
Size: 2042 202
BtsI GCAGTG_nn'
Cuts at: 0 132 1210 2244
Size: 132 1078 1034
Fragments arranged by size:
1078 1034 132
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1211 1246 2244
Size: 1211 35 998
Fragments arranged by size:
1211 998 35
EaeI y'GGCC_r
Cuts at: 0 510 713 1085 1925 2244
Size: 510 203 372 840 319
Fragments arranged by size:
840 510 372 319 203
EarI CTCTTCn'nnn_
Cuts at: 0 67 228 234 255 264 361 2244
Size: 67 161 6 21 9 97 1883
Fragments arranged by size:
1883 161 97 67 21 9 6
EcoICRI GAG'CTC
Cuts at: 0 441 1071 2244
Size: 441 630 1173
Fragments arranged by size:
1173 630 441
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 528 575 579 859 1710 2244
Size: 48 480 47 4 280 851 534
Fragments arranged by size:
851 534 480 280 48 47 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 115 2244
Size: 115 2129
EcoO109I rG'GnC_Cy
Cuts at: 0 148 792 1657 2244
Size: 148 644 865 587
Fragments arranged by size:
865 644 587 148
EcoRI G'AATT_C
Cuts at: 0 559 947 2244
Size: 559 388 1297
Fragments arranged by size:
1297 559 388
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1346 2244
Size: 1346 898
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 526 558 1434 1466 1901 1933 2244
Size: 526 32 876 32 435 32 311
Fragments arranged by size:
876 526 435 311 32 32 32
HincII GTy'rAC
Cuts at: 0 1619 1732 2244
Size: 1619 113 512
Fragments arranged by size:
1619 512 113
HindIII A'AGCT_T
Cuts at: 0 919 2244
Size: 919 1325
MfeI C'AATT_G
Cuts at: 0 1760 2244
Size: 1760 484
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 725 1353 1794 1899 2244
Size: 725 628 441 105 345
Fragments arranged by size:
725 628 441 345 105
MscI TGG'CCA
Cuts at: 0 1087 1927 2244
Size: 1087 840 317
Fragments arranged by size:
1087 840 317
MslI CAynn'nnrTG
Cuts at: 0 1628 2244
Size: 1628 616
MspA1I CmG'CkG
Cuts at: 0 53 273 1063 2244
Size: 53 220 790 1181
Fragments arranged by size:
1181 790 220 53
NcoI C'CATG_G
Cuts at: 0 2042 2244
Size: 2042 202
NsiI A_TGCA'T
Cuts at: 0 979 1134 1254 2244
Size: 979 155 120 990
Fragments arranged by size:
990 979 155 120
NspI r_CATG'y
Cuts at: 0 1161 1188 1887 1934 2244
Size: 1161 27 699 47 310
Fragments arranged by size:
1161 699 310 47 27
PciI A'CATG_T
Cuts at: 0 1883 1930 2244
Size: 1883 47 314
Fragments arranged by size:
1883 314 47
PflMI CCAn_nnn'nTGG
Cuts at: 0 1595 2244
Size: 1595 649
PfoI T'CCnGG_A
Cuts at: 0 405 2244
Size: 405 1839
PpuMI rG'GwC_Cy
Cuts at: 0 148 792 2244
Size: 148 644 1452
Fragments arranged by size:
1452 644 148
PsiI TTA'TAA
Cuts at: 0 1201 2244
Size: 1201 1043
PstI C_TGCA'G
Cuts at: 0 311 864 1493 2244
Size: 311 553 629 751
Fragments arranged by size:
751 629 553 311
PvuII CAG'CTG
Cuts at: 0 1063 2244
Size: 1063 1181
SacI G_AGCT'C
Cuts at: 0 443 1073 2244
Size: 443 630 1171
Fragments arranged by size:
1171 630 443
SanDI GG'GwC_CC
Cuts at: 0 148 2244
Size: 148 2096
ScaI AGT'ACT
Cuts at: 0 1649 2244
Size: 1649 595
SfcI C'TryA_G
Cuts at: 0 307 860 1367 1388 1424 1489 2244
Size: 307 553 507 21 36 65 755
Fragments arranged by size:
755 553 507 307 65 36 21
SgrAI Cr'CCGG_yG
Cuts at: 0 85 2244
Size: 85 2159
SmlI C'TyrA_G
Cuts at: 0 968 2244
Size: 968 1276
SphI G_CATG'C
Cuts at: 0 1188 2244
Size: 1188 1056
SspI AAT'ATT
Cuts at: 0 1855 2202 2244
Size: 1855 347 42
Fragments arranged by size:
1855 347 42
StyI C'CwwG_G
Cuts at: 0 2042 2091 2244
Size: 2042 49 153
Fragments arranged by size:
2042 153 49
TatI w'GTAC_w
Cuts at: 0 1647 2244
Size: 1647 597
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 273 305 1872 1899 1904 1931 2199 2231
Size: 273 32 1567 27 5 27 268 32
Cuts at: 2231 2244
Size: 13
Fragments arranged by size:
1567 273 268 32 32 27 27 13
5
Tth111I GACn'n_nGTC
Cuts at: 0 796 1631 2244
Size: 796 835 613
Fragments arranged by size:
835 796 613
XbaI T'CTAG_A
Cuts at: 0 1290 2244
Size: 1290 954
XmnI GAAnn'nnTTC
Cuts at: 0 61 2244
Size: 61 2183
Enzymes that do cut:
AarI AcuI AflIII AlfI AlwNI ApoI BamHI BanI
BanII BbvCI BcgI BfrBI BglI BlpI Bme1580I BmrI
BpmI Bpu10I BpuEI BsaAI BsaXI BseRI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BssSI
BstAPI BstYI BtgI BtsI CspCI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I HincII HindIII
MfeI MmeI MscI MslI MspA1I NcoI NsiI NspI
PciI PflMI PfoI PpuMI PsiI PstI PvuII SacI
SanDI ScaI SfcI SgrAI SmlI SphI SspI StyI
TatI TstI Tth111I XbaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BbeI BbsI BciVI BclI BglII BmgBI
BmtI BplI BsaI BsaBI BsaHI BsaWI BsiEI BsiWI
BsmI BspEI BsrBI BsrGI BssHII BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EagI
EciI EcoRV FseI FspI FspAI HaeII HpaI KasI
KpnI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PasI PmeI PmlI PpiI PshAI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SgfI SmaI SnaBI SpeI SrfI
StuI SwaI TaqII XcmI XhoI XmaI ZraI