Restriction map for
pF1KA1496
(Linear) (Six-base) MAPSORT of: KIAA1496.seq Check: 5241 from: 1 to: 3084
KIAA1496.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1254 1441 3084
Size: 1254 187 1643
Fragments arranged by size:
1643 1254 187
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 2105 2135 2195 2626 2858 2993 3084
Size: 331 1774 30 60 431 232 135 91
Fragments arranged by size:
1774 431 331 232 135 91 60 30
AflIII A'CryG_T
Cuts at: 0 583 3084
Size: 583 2501
AhdI GACnn_n'nnGTC
Cuts at: 0 2834 3084
Size: 2834 250
AleI CACnn'nnGTG
Cuts at: 0 586 1942 2752 3084
Size: 586 1356 810 332
Fragments arranged by size:
1356 810 586 332
AlwNI CAG_nnn'CTG
Cuts at: 0 397 1948 1981 2173 3084
Size: 397 1551 33 192 911
Fragments arranged by size:
1551 911 397 192 33
ApaI G_GGCC'C
Cuts at: 0 1620 3084
Size: 1620 1464
ApoI r'AATT_y
Cuts at: 0 309 366 900 1364 2014 3084
Size: 309 57 534 464 650 1070
Fragments arranged by size:
1070 650 534 464 309 57
AseI AT'TA_AT
Cuts at: 0 260 776 3084
Size: 260 516 2308
Fragments arranged by size:
2308 516 260
AvaI C'yCGr_G
Cuts at: 0 614 3084
Size: 614 2470
BamHI G'GATC_C
Cuts at: 0 2163 2243 3001 3084
Size: 2163 80 758 83
Fragments arranged by size:
2163 758 83 80
BanI G'GyrC_C
Cuts at: 0 536 1105 1512 3084
Size: 536 569 407 1572
Fragments arranged by size:
1572 569 536 407
BanII G_rGCy'C
Cuts at: 0 628 1202 1620 3084
Size: 628 574 418 1464
Fragments arranged by size:
1464 628 574 418
BbsI GAAGACnn'nnnn_
Cuts at: 0 1972 3084
Size: 1972 1112
BciVI GTATCCnnnnn_n'
Cuts at: 0 273 3084
Size: 273 2811
BclI T'GATC_A
Cuts at: 0 1688 3084
Size: 1688 1396
BfrBI ATG'CAT
Cuts at: 0 890 3084
Size: 890 2194
BlpI GC'TnA_GC
Cuts at: 0 1196 3084
Size: 1196 1888
Bme1580I G_kGCm'C
Cuts at: 0 539 1110 1317 1620 3084
Size: 539 571 207 303 1464
Fragments arranged by size:
1464 571 539 303 207
BmrI ACTGGGnnnn_n'
Cuts at: 0 1873 2365 2515 2702 3084
Size: 1873 492 150 187 382
Fragments arranged by size:
1873 492 382 187 150
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 949 3084
Size: 949 2135
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 470 1205 3084
Size: 470 735 1879
Fragments arranged by size:
1879 735 470
Bpu10I CC'TnA_GC
Cuts at: 0 48 3084
Size: 48 3036
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2405 3084
Size: 2405 679
BsaHI Gr'CG_yC
Cuts at: 0 930 3084
Size: 930 2154
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 774 804 2779 2809 3084
Size: 774 30 1975 30 275
Fragments arranged by size:
1975 774 275 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 399 1096 2141 3084
Size: 399 697 1045 943
Fragments arranged by size:
1045 943 697 399
BseYI C'CCAG_C
Cuts at: 0 94 621 2659 3084
Size: 94 527 2038 425
Fragments arranged by size:
2038 527 425 94
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1282 1369 3084
Size: 1282 87 1715
Fragments arranged by size:
1715 1282 87
BsiHKAI G_wGCw'C
Cuts at: 0 1202 3084
Size: 1202 1882
BsmI GAATG_Cn'
Cuts at: 0 892 1018 2742 3084
Size: 892 126 1724 342
Fragments arranged by size:
1724 892 342 126
BsmBI CGTCTCn'nnnn_
Cuts at: 0 937 3084
Size: 937 2147
Bsp1286I G_dGCh'C
Cuts at: 0 539 628 1110 1202 1317 1620 3084
Size: 539 89 482 92 115 303 1464
Fragments arranged by size:
1464 539 482 303 115 92 89
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 865 1254 1441 3084
Size: 865 389 187 1643
Fragments arranged by size:
1643 865 389 187
BsrDI GCAATG_nn'
Cuts at: 0 890 2499 3084
Size: 890 1609 585
Fragments arranged by size:
1609 890 585
BsrFI r'CCGG_y
Cuts at: 0 2138 2653 3084
Size: 2138 515 431
Fragments arranged by size:
2138 515 431
BssSI C'ACGA_G
Cuts at: 0 905 3084
Size: 905 2179
BstEII G'GTnAC_C
Cuts at: 0 2232 3084
Size: 2232 852
BstYI r'GATC_y
Cuts at: 0 470 1642 2163 2243 3001 3084
Size: 470 1172 521 80 758 83
Fragments arranged by size:
1172 758 521 470 83 80
Bsu36I CC'TnA_GG
Cuts at: 0 1949 3084
Size: 1949 1135
BtgI C'CryG_G
Cuts at: 0 10 989 1919 1999 2783 3084
Size: 10 979 930 80 784 301
Fragments arranged by size:
979 930 784 301 80 10
ClaI AT'CG_AT
Cuts at: 0 1958 3084
Size: 1958 1126
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1968 2003 3084
Size: 1968 35 1081
Fragments arranged by size:
1968 1081 35
DraI TTT'AAA
Cuts at: 0 372 3084
Size: 372 2712
EarI CTCTTCn'nnn_
Cuts at: 0 713 1326 2081 2393 2602 3084
Size: 713 613 755 312 209 482
Fragments arranged by size:
755 713 613 482 312 209
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2147 2209 3084
Size: 2147 62 875
Fragments arranged by size:
2147 875 62
EcoICRI GAG'CTC
Cuts at: 0 1200 3084
Size: 1200 1884
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 470 1205 2105 2135 2195 2626 2858
Size: 331 139 735 900 30 60 431 232
Cuts at: 2858 2993 3084
Size: 135 91
Fragments arranged by size:
900 735 431 331 232 139 135 91
60 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1308 3084
Size: 1308 1776
EcoO109I rG'GnC_Cy
Cuts at: 0 73 1616 1617 2601 3084
Size: 73 1543 1 984 483
Fragments arranged by size:
1543 984 483 73 1
EcoRI G'AATT_C
Cuts at: 0 900 3084
Size: 900 2184
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 361 1385 1523 2776 3084
Size: 361 1024 138 1253 308
Fragments arranged by size:
1253 1024 361 308 138
HaeII r_GCGC'y
Cuts at: 0 2660 2675 3084
Size: 2660 15 409
Fragments arranged by size:
2660 409 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2536 2568 2779 2811 2820 2852 3084
Size: 2536 32 211 32 9 32 232
Fragments arranged by size:
2536 232 211 32 32 32 9
MfeI C'AATT_G
Cuts at: 0 323 3084
Size: 323 2761
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 655 885 3084
Size: 490 165 230 2199
Fragments arranged by size:
2199 490 230 165
MslI CAynn'nnrTG
Cuts at: 0 586 1435 1942 2752 3084
Size: 586 849 507 810 332
Fragments arranged by size:
849 810 586 507 332
MspA1I CmG'CkG
Cuts at: 0 189 1581 1704 2878 3084
Size: 189 1392 123 1174 206
Fragments arranged by size:
1392 1174 206 189 123
NaeI GCC'GGC
Cuts at: 0 2655 3084
Size: 2655 429
NcoI C'CATG_G
Cuts at: 0 10 1999 2783 3084
Size: 10 1989 784 301
Fragments arranged by size:
1989 784 301 10
NdeI CA'TA_TG
Cuts at: 0 462 2319 3084
Size: 462 1857 765
Fragments arranged by size:
1857 765 462
NgoMIV G'CCGG_C
Cuts at: 0 2653 3084
Size: 2653 431
NsiI A_TGCA'T
Cuts at: 0 892 3084
Size: 892 2192
NspI r_CATG'y
Cuts at: 0 587 1149 3084
Size: 587 562 1935
Fragments arranged by size:
1935 587 562
PciI A'CATG_T
Cuts at: 0 583 3084
Size: 583 2501
PflMI CCAn_nnn'nTGG
Cuts at: 0 448 2253 2480 3084
Size: 448 1805 227 604
Fragments arranged by size:
1805 604 448 227
PfoI T'CCnGG_A
Cuts at: 0 1324 3084
Size: 1324 1760
PsiI TTA'TAA
Cuts at: 0 230 2342 3084
Size: 230 2112 742
Fragments arranged by size:
2112 742 230
PspOMI G'GGCC_C
Cuts at: 0 1616 3084
Size: 1616 1468
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2045 2077 3084
Size: 2045 32 1007
Fragments arranged by size:
2045 1007 32
PstI C_TGCA'G
Cuts at: 0 2394 3084
Size: 2394 690
PvuII CAG'CTG
Cuts at: 0 189 1704 2878 3084
Size: 189 1515 1174 206
Fragments arranged by size:
1515 1174 206 189
SacI G_AGCT'C
Cuts at: 0 1202 3084
Size: 1202 1882
SapI GCTCTTCn'nnn_
Cuts at: 0 2393 2602 3084
Size: 2393 209 482
Fragments arranged by size:
2393 482 209
ScaI AGT'ACT
Cuts at: 0 2855 3056 3084
Size: 2855 201 28
Fragments arranged by size:
2855 201 28
SexAI A'CCwGG_T
Cuts at: 0 1600 3084
Size: 1600 1484
SfcI C'TryA_G
Cuts at: 0 1982 2182 2390 2405 2823 3050 3084
Size: 1982 200 208 15 418 227 34
Fragments arranged by size:
1982 418 227 208 200 34 15
SmlI C'TyrA_G
Cuts at: 0 2420 3084
Size: 2420 664
StyI C'CwwG_G
Cuts at: 0 10 753 1309 1999 2479 2783 3084
Size: 10 743 556 690 480 304 301
Fragments arranged by size:
743 690 556 480 304 301 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 655 1992 2323 3084
Size: 655 1337 331 761
Fragments arranged by size:
1337 761 655 331
TatI w'GTAC_w
Cuts at: 0 388 2853 3054 3084
Size: 388 2465 201 30
Fragments arranged by size:
2465 388 201 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 236 268 774 806 819 851 2520 2552
Size: 236 32 506 32 13 32 1669 32
Cuts at: 2552 2940 2972 3084
Size: 388 32 112
Fragments arranged by size:
1669 506 388 236 112 32 32 32
32 32 13
XmnI GAAnn'nnTTC
Cuts at: 0 707 3084
Size: 707 2377
Enzymes that do cut:
AarI AcuI AflIII AhdI AleI AlwNI ApaI ApoI
AseI AvaI BamHI BanI BanII BbsI BciVI BclI
BfrBI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspMI BsrDI BsrFI BssSI BstEII BstYI Bsu36I
BtgI ClaI CspCI DraI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I MfeI MmeI
MslI MspA1I NaeI NcoI NdeI NgoMIV NsiI NspI
PciI PflMI PfoI PsiI PspOMI PsrI PstI PvuII
SacI SapI ScaI SexAI SfcI SmlI StyI TaqII
TatI TstI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AlfI
AloI ApaLI AscI AsiSI AvrII BaeI BbeI BbvCI
BcgI BglI BglII BmgBI BmtI BsaI BsaAI BsaBI
BsaWI BsiEI BsiWI BspEI BspHI BsrBI BsrGI BssHII
BstAPI BstBI BstXI BstZ17I BtgZI BtsI DraIII DrdI
EaeI EagI EcoRV FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MluI MscI NarI NheI NotI
NruI PacI PasI PmeI PmlI PpiI PpuMI PshAI
PspXI PvuI RsrII SacII SalI SanDI SbfI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaI
ZraI