Restriction map for
pF1KA1497
(Linear) (Six-base) MAPSORT of: KIAA1497.seq Check: 8013 from: 1 to: 2148
KIAA1497.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AatII G_ACGT'C
Cuts at: 0 1654 2148
Size: 1654 494
AccI GT'mk_AC
Cuts at: 0 414 908 2120 2148
Size: 414 494 1212 28
Fragments arranged by size:
1212 494 414 28
Acc65I G'GTAC_C
Cuts at: 0 1444 1567 2148
Size: 1444 123 581
Fragments arranged by size:
1444 581 123
AflIII A'CryG_T
Cuts at: 0 1492 2141 2148
Size: 1492 649 7
Fragments arranged by size:
1492 649 7
AleI CACnn'nnGTG
Cuts at: 0 223 1528 2148
Size: 223 1305 620
Fragments arranged by size:
1305 620 223
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1250 1282 2148
Size: 1250 32 866
Fragments arranged by size:
1250 866 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1363 2148
Size: 1363 785
ApoI r'AATT_y
Cuts at: 0 116 581 614 645 789 796 1367 2148
Size: 116 465 33 31 144 7 571 781
Fragments arranged by size:
781 571 465 144 116 33 31 7
AvaI C'yCGr_G
Cuts at: 0 1526 2148
Size: 1526 622
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1479 1512 1559 1592 2148
Size: 1479 33 47 33 556
Fragments arranged by size:
1479 556 47 33 33
BanI G'GyrC_C
Cuts at: 0 1220 1444 1567 2148
Size: 1220 224 123 581
Fragments arranged by size:
1220 581 224 123
BanII G_rGCy'C
Cuts at: 0 899 1181 2148
Size: 899 282 967
Fragments arranged by size:
967 899 282
BbsI GAAGACnn'nnnn_
Cuts at: 0 65 262 1481 2148
Size: 65 197 1219 667
Fragments arranged by size:
1219 667 197 65
BbvCI CC'TCA_GC
Cuts at: 0 424 2148
Size: 424 1724
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 231 265 898 932 2148
Size: 231 34 633 34 1216
Fragments arranged by size:
1216 633 231 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1111 2148
Size: 1111 1037
BclI T'GATC_A
Cuts at: 0 1872 2148
Size: 1872 276
BlpI GC'TnA_GC
Cuts at: 0 1352 2148
Size: 1352 796
Bme1580I G_kGCm'C
Cuts at: 0 1223 2148
Size: 1223 925
BmrI ACTGGGnnnn_n'
Cuts at: 0 194 883 1383 1717 2148
Size: 194 689 500 334 431
Fragments arranged by size:
689 500 431 334 194
Bpu10I CC'TnA_GC
Cuts at: 0 424 2148
Size: 424 1724
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 742 2148
Size: 742 1406
BsaBI GATnn'nnATC
Cuts at: 0 1099 2148
Size: 1099 1049
BsaHI Gr'CG_yC
Cuts at: 0 1651 2148
Size: 1651 497
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 508 538 1168 1198 1250 1280 2148
Size: 508 30 630 30 52 30 868
Fragments arranged by size:
868 630 508 52 30 30 30
BseYI C'CCAG_C
Cuts at: 0 42 356 2148
Size: 42 314 1792
Fragments arranged by size:
1792 314 42
BsiEI CG_ry'CG
Cuts at: 0 912 2148
Size: 912 1236
BsiHKAI G_wGCw'C
Cuts at: 0 899 2148
Size: 899 1249
BsmI GAATG_Cn'
Cuts at: 0 1053 2148
Size: 1053 1095
Bsp1286I G_dGCh'C
Cuts at: 0 899 1181 1223 2148
Size: 899 282 42 925
Fragments arranged by size:
925 899 282 42
BspHI T'CATG_A
Cuts at: 0 587 1646 2148
Size: 587 1059 502
Fragments arranged by size:
1059 587 502
BsrDI GCAATG_nn'
Cuts at: 0 180 1940 2148
Size: 180 1760 208
Fragments arranged by size:
1760 208 180
Bsu36I CC'TnA_GG
Cuts at: 0 1117 2148
Size: 1117 1031
BtgI C'CryG_G
Cuts at: 0 1346 2148
Size: 1346 802
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 234 2148
Size: 234 1914
BtsI GCAGTG_nn'
Cuts at: 0 1865 2148
Size: 1865 283
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 147 182 2148
Size: 147 35 1966
Fragments arranged by size:
1966 147 35
DraIII CAC_nnn'GTG
Cuts at: 0 1227 2148
Size: 1227 921
DrdI GACnn_nn'nnGTC
Cuts at: 0 1818 2148
Size: 1818 330
EarI CTCTTCn'nnn_
Cuts at: 0 2022 2148
Size: 2022 126
EcoICRI GAG'CTC
Cuts at: 0 897 2148
Size: 897 1251
EcoO109I rG'GnC_Cy
Cuts at: 0 1553 1719 2148
Size: 1553 166 429
Fragments arranged by size:
1553 429 166
EcoRI G'AATT_C
Cuts at: 0 614 2148
Size: 614 1534
EcoRV GAT'ATC
Cuts at: 0 1277 1314 2148
Size: 1277 37 834
Fragments arranged by size:
1277 834 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 715 747 1168 1200 2148
Size: 715 32 421 32 948
Fragments arranged by size:
948 715 421 32 32
HincII GTy'rAC
Cuts at: 0 143 909 1548 2121 2148
Size: 143 766 639 573 27
Fragments arranged by size:
766 639 573 143 27
HindIII A'AGCT_T
Cuts at: 0 654 1020 2148
Size: 654 366 1128
Fragments arranged by size:
1128 654 366
HpaI GTT'AAC
Cuts at: 0 1548 2148
Size: 1548 600
KpnI G_GTAC'C
Cuts at: 0 1448 1571 2148
Size: 1448 123 577
Fragments arranged by size:
1448 577 123
MfeI C'AATT_G
Cuts at: 0 34 2148
Size: 34 2114
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 551 1175 2148
Size: 551 624 973
Fragments arranged by size:
973 624 551
MslI CAynn'nnrTG
Cuts at: 0 223 1528 1618 1945 2148
Size: 223 1305 90 327 203
Fragments arranged by size:
1305 327 223 203 90
NcoI C'CATG_G
Cuts at: 0 1346 2148
Size: 1346 802
NruI TCG'CGA
Cuts at: 0 253 2148
Size: 253 1895
NspI r_CATG'y
Cuts at: 0 1496 2145 2148
Size: 1496 649 3
Fragments arranged by size:
1496 649 3
PciI A'CATG_T
Cuts at: 0 1492 2141 2148
Size: 1492 649 7
Fragments arranged by size:
1492 649 7
PflMI CCAn_nnn'nTGG
Cuts at: 0 990 2148
Size: 990 1158
PfoI T'CCnGG_A
Cuts at: 0 690 1244 1624 2148
Size: 690 554 380 524
Fragments arranged by size:
690 554 524 380
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1250 1282 2148
Size: 1250 32 866
Fragments arranged by size:
1250 866 32
PpuMI rG'GwC_Cy
Cuts at: 0 1553 1719 2148
Size: 1553 166 429
Fragments arranged by size:
1553 429 166
PstI C_TGCA'G
Cuts at: 0 275 1760 1902 2100 2148
Size: 275 1485 142 198 48
Fragments arranged by size:
1485 275 198 142 48
SacI G_AGCT'C
Cuts at: 0 899 2148
Size: 899 1249
SalI G'TCGA_C
Cuts at: 0 907 2119 2148
Size: 907 1212 29
Fragments arranged by size:
1212 907 29
SanDI GG'GwC_CC
Cuts at: 0 1553 1719 2148
Size: 1553 166 429
Fragments arranged by size:
1553 429 166
SexAI A'CCwGG_T
Cuts at: 0 1223 2148
Size: 1223 925
SfcI C'TryA_G
Cuts at: 0 271 1756 1898 2096 2148
Size: 271 1485 142 198 52
Fragments arranged by size:
1485 271 198 142 52
SmlI C'TyrA_G
Cuts at: 0 721 2148
Size: 721 1427
SspI AAT'ATT
Cuts at: 0 1800 2148
Size: 1800 348
StyI C'CwwG_G
Cuts at: 0 1346 1448 1512 2148
Size: 1346 102 64 636
Fragments arranged by size:
1346 636 102 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 585 2148
Size: 585 1563
TatI w'GTAC_w
Cuts at: 0 1885 1949 2148
Size: 1885 64 199
Fragments arranged by size:
1885 199 64
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 508 540 672 704 2148
Size: 508 32 132 32 1444
Fragments arranged by size:
1444 508 132 32 32
ZraI GAC'GTC
Cuts at: 0 1652 2148
Size: 1652 496
Enzymes that do cut:
AatII AccI Acc65I AflIII AleI AloI AlwNI ApoI
AvaI BaeI BanI BanII BbsI BbvCI BcgI BciVI
BclI BlpI Bme1580I BmrI Bpu10I BpuEI BsaBI BsaHI
BsaXI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI BsrDI
Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI EarI
EcoICRI EcoO109I EcoRI EcoRV Hin4I HincII HindIII HpaI
KpnI MfeI MmeI MslI NcoI NruI NspI PciI
PflMI PfoI PpiI PpuMI PstI SacI SalI SanDI
SexAI SfcI SmlI SspI StyI TaqII TatI TstI
ZraI
Enzymes that do not cut:
AarI AclI AcuI AfeI AflII AgeI AhdI AlfI
ApaI ApaLI AscI AseI AsiSI AvrII BamHI BbeI
BfrBI BglI BglII BmgBI BmtI BplI BpmI BsaI
BsaAI BsaWI BseRI BsgI BsiWI BsmBI BspEI BspMI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I ClaI DraI EaeI EagI EciI
Eco57MI EcoNI FalI FseI FspI FspAI HaeII KasI
MluI MscI MspA1I NaeI NarI NdeI NgoMIV NheI
NotI NsiI PacI PasI PmeI PmlI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacII SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI SwaI Tth111I XbaI XcmI
XhoI XmaI XmnI