Restriction map for
pF1KSDA1498
(Linear) (Six-base) MAPSORT of: KIAA1498.seq Check: 6259 from: 1 to: 9129
KIAA1498.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 445 831 1884 6669 6840 7899 8898 8964
Size: 445 386 1053 4785 171 1059 999 66
Cuts at: 8964 9129
Size: 165
Fragments arranged by size:
4785 1059 1053 999 445 386 171 165
66
AatII G_ACGT'C
Cuts at: 0 943 3071 5394 9129
Size: 943 2128 2323 3735
Fragments arranged by size:
3735 2323 2128 943
AccI GT'mk_AC
Cuts at: 0 6350 9129
Size: 6350 2779
AclI AA'CG_TT
Cuts at: 0 3372 9129
Size: 3372 5757
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1156 3104 4102 4211 4285 5353 5405 6271
Size: 1156 1948 998 109 74 1068 52 866
Cuts at: 6271 6691 8572 8578 9129
Size: 420 1881 6 551
Fragments arranged by size:
1948 1881 1156 1068 998 866 551 420
109 74 52 6
AfeI AGC'GCT
Cuts at: 0 321 9129
Size: 321 8808
AflII C'TTAA_G
Cuts at: 0 8701 9129
Size: 8701 428
AflIII A'CryG_T
Cuts at: 0 7130 9129
Size: 7130 1999
AhdI GACnn_n'nnGTC
Cuts at: 0 401 5099 7731 9129
Size: 401 4698 2632 1398
Fragments arranged by size:
4698 2632 1398 401
AleI CACnn'nnGTG
Cuts at: 0 1012 3076 3520 5611 6823 7771 8284 9129
Size: 1012 2064 444 2091 1212 948 513 845
Fragments arranged by size:
2091 2064 1212 1012 948 845 513 444
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1655 3287 3991 4975 8693 9129
Size: 1655 1632 704 984 3718 436
Fragments arranged by size:
3718 1655 1632 984 704 436
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 602 634 9129
Size: 602 32 8495
Fragments arranged by size:
8495 602 32
AlwNI CAG_nnn'CTG
Cuts at: 0 56 180 756 846 1104 1134 4599 5241
Size: 56 124 576 90 258 30 3465 642
Cuts at: 5241 5274 5514 5835 6058 6798 7510 8124 8547
Size: 33 240 321 223 740 712 614 423
Cuts at: 8547 8550 8745 9129
Size: 3 195 384
Fragments arranged by size:
3465 740 712 642 614 576 423 384
321 258 240 223 195 124 90 56
33 30 3
ApaI G_GGCC'C
Cuts at: 0 241 3316 3732 4393 8614 8757 9129
Size: 241 3075 416 661 4221 143 372
Fragments arranged by size:
4221 3075 661 416 372 241 143
ApaLI G'TGCA_C
Cuts at: 0 3676 4705 7672 8533 9129
Size: 3676 1029 2967 861 596
Fragments arranged by size:
3676 2967 1029 861 596
ApoI r'AATT_y
Cuts at: 0 2604 6629 9129
Size: 2604 4025 2500
Fragments arranged by size:
4025 2604 2500
AvaI C'yCGr_G
Cuts at: 0 633 907 1672 2513 3110 4309 4639 6745
Size: 633 274 765 841 597 1199 330 2106
Cuts at: 6745 8315 9129
Size: 1570 814
Fragments arranged by size:
2106 1570 1199 841 814 765 633 597
330 274
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 4042 4075 5559 5592 9129
Size: 4042 33 1484 33 3537
Fragments arranged by size:
4042 3537 1484 33 33
BanI G'GyrC_C
Cuts at: 0 147 457 694 2866 4022 4711 5187 6681
Size: 147 310 237 2172 1156 689 476 1494
Cuts at: 6681 7531 7836 8263 8278 8364 9033 9129
Size: 850 305 427 15 86 669 96
Fragments arranged by size:
2172 1494 1156 850 689 669 476 427
310 305 237 147 96 86 15
BanII G_rGCy'C
Cuts at: 0 196 241 550 593 615 724 3162 3316
Size: 196 45 309 43 22 109 2438 154
Cuts at: 3316 3665 3718 3732 4010 4019 4393 4662 6308
Size: 349 53 14 278 9 374 269 1646
Cuts at: 6308 8230 8614 8757 9129
Size: 1922 384 143 372
Fragments arranged by size:
2438 1922 1646 384 374 372 349 309
278 269 196 154 143 109 53 45
43 22 14 9
BbeI G_GCGC'C
Cuts at: 0 2870 4715 9129
Size: 2870 1845 4414
Fragments arranged by size:
4414 2870 1845
BbsI GAAGACnn'nnnn_
Cuts at: 0 1137 1789 6259 9129
Size: 1137 652 4470 2870
Fragments arranged by size:
4470 2870 1137 652
BbvCI CC'TCA_GC
Cuts at: 0 132 2076 2254 5317 5885 6537 9129
Size: 132 1944 178 3063 568 652 2592
Fragments arranged by size:
3063 2592 1944 652 568 178 132
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 125 159 5470 5504 9129
Size: 125 34 5311 34 3625
Fragments arranged by size:
5311 3625 125 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2190 4070 9129
Size: 2190 1880 5059
Fragments arranged by size:
5059 2190 1880
BclI T'GATC_A
Cuts at: 0 7239 9129
Size: 7239 1890
BglI GCCn_nnn'nGGC
Cuts at: 0 1677 2509 7368 7483 8383 9129
Size: 1677 832 4859 115 900 746
Fragments arranged by size:
4859 1677 900 832 746 115
BglII A'GATC_T
Cuts at: 0 4116 5760 6989 9129
Size: 4116 1644 1229 2140
Fragments arranged by size:
4116 2140 1644 1229
BlpI GC'TnA_GC
Cuts at: 0 323 2828 4469 4508 6482 6725 9129
Size: 323 2505 1641 39 1974 243 2404
Fragments arranged by size:
2505 2404 1974 1641 323 243 39
Bme1580I G_kGCm'C
Cuts at: 0 241 462 697 983 3316 3680 3732 3950
Size: 241 221 235 286 2333 364 52 218
Cuts at: 3950 4393 4709 4977 7676 7841 8268 8281 8537
Size: 443 316 268 2699 165 427 13 256
Cuts at: 8537 8614 8757 9038 9129
Size: 77 143 281 91
Fragments arranged by size:
2699 2333 443 427 364 316 286 281
268 256 241 235 221 218 165 143
91 77 52 13
BmgBI CAC'GTC
Cuts at: 0 371 1010 3468 3479 9129
Size: 371 639 2458 11 5650
Fragments arranged by size:
5650 2458 639 371 11
BmrI ACTGGGnnnn_n'
Cuts at: 0 147 155 410 2334 3594 3728 6777 7520
Size: 147 8 255 1924 1260 134 3049 743
Cuts at: 7520 7804 8046 8154 8703 9129
Size: 284 242 108 549 426
Fragments arranged by size:
3049 1924 1260 743 549 426 284 255
242 147 134 108 8
BmtI G_CTAG'C
Cuts at: 0 2714 6106 9129
Size: 2714 3392 3023
Fragments arranged by size:
3392 3023 2714
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2177 3737 4236 7885 9129
Size: 2177 1560 499 3649 1244
Fragments arranged by size:
3649 2177 1560 1244 499
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 537 610 1501 2042 2084 2242 2359
Size: 287 250 73 891 541 42 158 117
Cuts at: 2359 3679 3684 4075 4111 4606 4925 6007 6307
Size: 1320 5 391 36 495 319 1082 300
Cuts at: 6307 6820 8281 8986 8993 9129
Size: 513 1461 705 7 136
Fragments arranged by size:
1461 1320 1082 891 705 541 513 495
391 319 300 287 250 158 136 117
73 42 36 7 5
Bpu10I CC'TnA_GC
Cuts at: 0 132 2076 2254 3712 3964 4963 5312 5317
Size: 132 1944 178 1458 252 999 349 5
Cuts at: 5317 5657 5783 5885 6537 9129
Size: 340 126 102 652 2592
Fragments arranged by size:
2592 1944 1458 999 652 349 340 252
178 132 126 102 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 564 3334 4919 5250 5953 8780 9129
Size: 564 2770 1585 331 703 2827 349
Fragments arranged by size:
2827 2770 1585 703 564 349 331
BsaI GGTCTCn'nnnn_
Cuts at: 0 353 657 2634 8433 9129
Size: 353 304 1977 5799 696
Fragments arranged by size:
5799 1977 696 353 304
BsaAI yAC'GTr
Cuts at: 0 4041 6501 9129
Size: 4041 2460 2628
Fragments arranged by size:
4041 2628 2460
BsaBI GATnn'nnATC
Cuts at: 0 3034 9129
Size: 3034 6095
BsaHI Gr'CG_yC
Cuts at: 0 940 2283 2867 3068 4519 4712 5391 7612
Size: 940 1343 584 201 1451 193 679 2221
Cuts at: 7612 8089 8989 9129
Size: 477 900 140
Fragments arranged by size:
2221 1451 1343 940 900 679 584 477
201 193 140
BsaWI w'CCGG_w
Cuts at: 0 1074 4434 5157 8400 9129
Size: 1074 3360 723 3243 729
Fragments arranged by size:
3360 3243 1074 729 723
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 269 299 602 632 1608 1638 2335 2365
Size: 269 30 303 30 976 30 697 30
Cuts at: 2365 2368 2398 2509 2539 3700 3730 4048 4078
Size: 3 30 111 30 1161 30 318 30
Cuts at: 4078 4442 4472 5274 5304 9102 9129
Size: 364 30 802 30 3798 27
Fragments arranged by size:
3798 1161 976 802 697 364 318 303
269 111 30 30 30 30 30 30
30 30 30 30 27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 91 1069 1297 1300 1303 1306 1315 1704
Size: 91 978 228 3 3 3 9 389
Cuts at: 1704 2356 2394 2452 2779 3822 5535 5902 6004
Size: 652 38 58 327 1043 1713 367 102
Cuts at: 6004 6112 6310 6578 6961 7086 8421 9129
Size: 108 198 268 383 125 1335 708
Fragments arranged by size:
1713 1335 1043 978 708 652 389 383
367 327 268 228 198 125 108 102
91 58 38 9 3 3 3
BseYI C'CCAG_C
Cuts at: 0 289 383 474 671 913 1065 1686 1819
Size: 289 94 91 197 242 152 621 133
Cuts at: 1819 3244 3249 4236 4333 4744 6024 7022 7271
Size: 1425 5 987 97 411 1280 998 249
Cuts at: 7271 7286 7394 7412 7434 7575 7679 7707 8058
Size: 15 108 18 22 141 104 28 351
Cuts at: 8058 8231 8369 8525 8756 8852 9024 9129
Size: 173 138 156 231 96 172 105
Fragments arranged by size:
1425 1280 998 987 621 411 351 289
249 242 231 197 173 172 156 152
141 138 133 108 105 104 97 96
94 91 28 22 18 15 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 473 1651 1796 1838 1912 1918 3283 3628
Size: 473 1178 145 42 74 6 1365 345
Cuts at: 3628 4312 4736 5347 7223 7447 7493 7834 7877
Size: 684 424 611 1876 224 46 341 43
Cuts at: 7877 8218 8588 8785 8932 9129
Size: 341 370 197 147 197
Fragments arranged by size:
1876 1365 1178 684 611 473 424 370
345 341 341 224 197 197 147 145
74 46 43 42 6
BsiEI CG_ry'CG
Cuts at: 0 1535 2899 4408 4736 5197 6581 7265 7787
Size: 1535 1364 1509 328 461 1384 684 522
Cuts at: 7787 8486 8900 9129
Size: 699 414 229
Fragments arranged by size:
1535 1509 1384 1364 699 684 522 461
414 328 229
BsiHKAI G_wGCw'C
Cuts at: 0 3111 3665 3680 3718 4709 5252 6308 7676
Size: 3111 554 15 38 991 543 1056 1368
Cuts at: 7676 8537 9129
Size: 861 592
Fragments arranged by size:
3111 1368 1056 991 861 592 554 543
38 15
BsiWI C'GTAC_G
Cuts at: 0 6979 9129
Size: 6979 2150
BsmI GAATG_Cn'
Cuts at: 0 1553 2172 4803 7014 9129
Size: 1553 619 2631 2211 2115
Fragments arranged by size:
2631 2211 2115 1553 619
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1378 4510 9129
Size: 1378 3132 4619
Fragments arranged by size:
4619 3132 1378
Bsp1286I G_dGCh'C
Cuts at: 0 196 241 462 550 593 615 697 724
Size: 196 45 221 88 43 22 82 27
Cuts at: 724 983 3111 3162 3316 3665 3680 3718 3732
Size: 259 2128 51 154 349 15 38 14
Cuts at: 3732 3950 4010 4019 4393 4662 4709 4977 5252
Size: 218 60 9 374 269 47 268 275
Cuts at: 5252 6308 7676 7841 8230 8268 8281 8537 8614
Size: 1056 1368 165 389 38 13 256 77
Cuts at: 8614 8757 9038 9129
Size: 143 281 91
Fragments arranged by size:
2128 1368 1056 389 374 349 281 275
269 268 259 256 221 218 196 165
154 143 91 88 82 77 60 51
47 45 43 38 38 27 22 15
14 13 9
BspEI T'CCGG_A
Cuts at: 0 1074 4434 9129
Size: 1074 3360 4695
Fragments arranged by size:
4695 3360 1074
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 378 445 831 1884 2254 4265 6669 6840
Size: 378 67 386 1053 370 2011 2404 171
Cuts at: 6840 7899 8898 8964 9129
Size: 1059 999 66 165
Fragments arranged by size:
2404 2011 1059 1053 999 386 378 370
171 165 67 66
BsrBI CCG'CTC
Cuts at: 0 285 660 3944 4923 6387 6513 6896 7762
Size: 285 375 3284 979 1464 126 383 866
Cuts at: 7762 9000 9129
Size: 1238 129
Fragments arranged by size:
3284 1464 1238 979 866 383 375 285
129 126
BsrDI GCAATG_nn'
Cuts at: 0 2243 3059 3269 5930 9129
Size: 2243 816 210 2661 3199
Fragments arranged by size:
3199 2661 2243 816 210
BsrFI r'CCGG_y
Cuts at: 0 84 742 1690 4521 4735 7260 8694 9129
Size: 84 658 948 2831 214 2525 1434 435
Fragments arranged by size:
2831 2525 1434 948 658 435 214 84
BsrGI T'GTAC_A
Cuts at: 0 5207 7028 9129
Size: 5207 1821 2101
Fragments arranged by size:
5207 2101 1821
BssHII G'CGCG_C
Cuts at: 0 8663 9129
Size: 8663 466
BssSI C'ACGA_G
Cuts at: 0 2434 3518 4165 6941 7032 9129
Size: 2434 1084 647 2776 91 2097
Fragments arranged by size:
2776 2434 2097 1084 647 91
BstAPI GCAn_nnn'nTGC
Cuts at: 0 39 438 447 843 846 1413 1527 6681
Size: 39 399 9 396 3 567 114 5154
Cuts at: 6681 8124 8547 8910 9129
Size: 1443 423 363 219
Fragments arranged by size:
5154 1443 567 423 399 396 363 219
114 39 9 3
BstEII G'GTnAC_C
Cuts at: 0 1465 8979 9129
Size: 1465 7514 150
Fragments arranged by size:
7514 1465 150
BstXI CCAn_nnnn'nTGG
Cuts at: 0 13 525 1428 7188 9129
Size: 13 512 903 5760 1941
Fragments arranged by size:
5760 1941 903 512 13
BstYI r'GATC_y
Cuts at: 0 3695 4116 5225 5760 6852 6989 8036 8774
Size: 3695 421 1109 535 1092 137 1047 738
Cuts at: 8774 9129
Size: 355
Fragments arranged by size:
3695 1109 1092 1047 738 535 421 355
137
BtgI C'CryG_G
Cuts at: 0 6 731 779 1616 1975 2507 3671 4198
Size: 6 725 48 837 359 532 1164 527
Cuts at: 4198 4322 4367 4986 5191 5285 5801 5855 7265
Size: 124 45 619 205 94 516 54 1410
Cuts at: 7265 7690 7712 7736 7787 8285 8516 8819 8984
Size: 425 22 24 51 498 231 303 165
Cuts at: 8984 9129
Size: 145
Fragments arranged by size:
1410 1164 837 725 619 532 527 516
498 425 359 303 231 205 165 145
124 94 54 51 48 45 24 22
6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1426 4398 7548 9129
Size: 1426 2972 3150 1581
Fragments arranged by size:
3150 2972 1581 1426
BtsI GCAGTG_nn'
Cuts at: 0 277 1105 1657 1700 2356 3071 3111 4528
Size: 277 828 552 43 656 715 40 1417
Cuts at: 4528 5502 6218 6230 7476 8933 9129
Size: 974 716 12 1246 1457 196
Fragments arranged by size:
1457 1417 1246 974 828 716 715 656
552 277 196 43 40 12
ClaI AT'CG_AT
Cuts at: 0 5480 9129
Size: 5480 3649
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2349 2384 3274 3309 9129
Size: 2349 35 890 35 5820
Fragments arranged by size:
5820 2349 890 35 35
DraI TTT'AAA
Cuts at: 0 6149 9129
Size: 6149 2980
DraIII CAC_nnn'GTG
Cuts at: 0 570 3786 9129
Size: 570 3216 5343
Fragments arranged by size:
5343 3216 570
DrdI GACnn_nn'nnGTC
Cuts at: 0 8988 9129
Size: 8988 141
EaeI y'GGCC_r
Cuts at: 0 758 2357 2896 3197 4299 4405 4733 5019
Size: 758 1599 539 301 1102 106 328 286
Cuts at: 5019 5194 7262 7784 8288 8747 8822 8897 9129
Size: 175 2068 522 504 459 75 75 232
Fragments arranged by size:
2068 1599 1102 758 539 522 504 459
328 301 286 232 175 106 75 75
EagI C'GGCC_G
Cuts at: 0 2896 4405 4733 5194 7262 7784 8897 9129
Size: 2896 1509 328 461 2068 522 1113 232
Fragments arranged by size:
2896 2068 1509 1113 522 461 328 232
EarI CTCTTCn'nnn_
Cuts at: 0 1274 1292 1998 2468 2741 2756 3734 3934
Size: 1274 18 706 470 273 15 978 200
Cuts at: 3934 4038 5726 6903 6949 9129
Size: 104 1688 1177 46 2180
Fragments arranged by size:
2180 1688 1274 1177 978 706 470 273
200 104 46 18 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1513 2508 4623 5556 5617 6316 9129
Size: 1513 995 2115 933 61 699 2813
Fragments arranged by size:
2813 2115 1513 995 933 699 61
EcoICRI GAG'CTC
Cuts at: 0 3663 3716 6306 9129
Size: 3663 53 2590 2823
Fragments arranged by size:
3663 2823 2590 53
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 537 610 1156 1501 2042 2084 2242
Size: 287 250 73 546 345 541 42 158
Cuts at: 2242 2359 3104 3679 3684 4075 4102 4111 4211
Size: 117 745 575 5 391 27 9 100
Cuts at: 4211 4285 4606 4925 5353 5405 6007 6271 6307
Size: 74 321 319 428 52 602 264 36
Cuts at: 6307 6691 6820 8281 8572 8578 8986 8993 9129
Size: 384 129 1461 291 6 408 7 136
Fragments arranged by size:
1461 745 602 575 546 541 428 408
391 384 345 321 319 291 287 264
250 158 136 129 117 100 74 73
52 42 36 27 9 7 6 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3097 5596 9129
Size: 3097 2499 3533
Fragments arranged by size:
3533 3097 2499
EcoO109I rG'GnC_Cy
Cuts at: 0 53 238 712 1621 2035 2304 3312 3313
Size: 53 185 474 909 414 269 1008 1
Cuts at: 3313 3501 3704 3728 3729 4389 5009 5238 5964
Size: 188 203 24 1 660 620 229 726
Cuts at: 5964 7431 7572 7631 8610 8753 9084 9129
Size: 1467 141 59 979 143 331 45
Fragments arranged by size:
1467 1008 979 909 726 660 620 474
414 331 269 229 203 188 185 143
141 59 53 45 24 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1161 1267 2616 4135 9129
Size: 1161 106 1349 1519 4994
Fragments arranged by size:
4994 1519 1349 1161 106
FseI GG_CCGG'CC
Cuts at: 0 746 9129
Size: 746 8383
HaeII r_GCGC'y
Cuts at: 0 323 2870 4703 4715 4896 4929 6775 7608
Size: 323 2547 1833 12 181 33 1846 833
Cuts at: 7608 8881 9017 9129
Size: 1273 136 112
Fragments arranged by size:
2547 1846 1833 1273 833 323 181 136
112 33 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 331 363 387 419 1116 1148 1578 1610
Size: 331 32 24 32 697 32 430 32
Cuts at: 1610 3377 3409 4048 4080 4165 4197 4674 4706
Size: 1767 32 639 32 85 32 477 32
Cuts at: 4706 5085 5117 5274 5306 6031 6063 7717 7749
Size: 379 32 157 32 725 32 1654 32
Cuts at: 7749 8001 8033 9129
Size: 252 32 1096
Fragments arranged by size:
1767 1654 1096 725 697 639 477 430
379 331 252 157 85 32 32 32
32 32 32 32 32 32 32 32
32 32 24
HincII GTy'rAC
Cuts at: 0 3957 4917 5093 6873 9129
Size: 3957 960 176 1780 2256
Fragments arranged by size:
3957 2256 1780 960 176
HindIII A'AGCT_T
Cuts at: 0 5351 9129
Size: 5351 3778
HpaI GTT'AAC
Cuts at: 0 6873 9129
Size: 6873 2256
KasI G'GCGC_C
Cuts at: 0 2866 4711 9129
Size: 2866 1845 4418
Fragments arranged by size:
4418 2866 1845
MfeI C'AATT_G
Cuts at: 0 2756 6131 9129
Size: 2756 3375 2998
Fragments arranged by size:
3375 2998 2756
MluI A'CGCG_T
Cuts at: 0 7130 9129
Size: 7130 1999
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 5327 5408 9071 9129
Size: 5327 81 3663 58
Fragments arranged by size:
5327 3663 81 58
MscI TGG'CCA
Cuts at: 0 760 2359 3199 4301 8290 8824 9129
Size: 760 1599 840 1102 3989 534 305
Fragments arranged by size:
3989 1599 1102 840 760 534 305
MslI CAynn'nnrTG
Cuts at: 0 1012 1027 1426 3076 3520 3973 5611 6038
Size: 1012 15 399 1650 444 453 1638 427
Cuts at: 6038 6823 7186 7771 8107 8284 9129
Size: 785 363 585 336 177 845
Fragments arranged by size:
1650 1638 1012 845 785 585 453 444
427 399 363 336 177 15
MspA1I CmG'CkG
Cuts at: 0 39 180 733 825 861 925 1668 1860
Size: 39 141 553 92 36 64 743 192
Cuts at: 1860 3642 4200 4473 4588 4599 4605 5193 5274
Size: 1782 558 273 115 11 6 588 81
Cuts at: 5274 6541 6724 7026 7173 7329 7348 7378 7579
Size: 1267 183 302 147 156 19 30 201
Cuts at: 7579 8334 8422 8547 9129
Size: 755 88 125 582
Fragments arranged by size:
1782 1267 755 743 588 582 558 553
302 273 201 192 183 156 147 141
125 115 92 88 81 64 39 36
30 19 11 6
NaeI GCC'GGC
Cuts at: 0 86 744 4737 7262 8696 9129
Size: 86 658 3993 2525 1434 433
Fragments arranged by size:
3993 2525 1434 658 433 86
NarI GG'CG_CC
Cuts at: 0 2867 4712 9129
Size: 2867 1845 4417
Fragments arranged by size:
4417 2867 1845
NcoI C'CATG_G
Cuts at: 0 6 3671 4986 5801 5855 7736 9129
Size: 6 3665 1315 815 54 1881 1393
Fragments arranged by size:
3665 1881 1393 1315 815 54 6
NgoMIV G'CCGG_C
Cuts at: 0 84 742 4735 7260 8694 9129
Size: 84 658 3993 2525 1434 435
Fragments arranged by size:
3993 2525 1434 658 435 84
NheI G'CTAG_C
Cuts at: 0 2710 6102 9129
Size: 2710 3392 3027
Fragments arranged by size:
3392 3027 2710
NotI GC'GGCC_GC
Cuts at: 0 5194 9129
Size: 5194 3935
NspI r_CATG'y
Cuts at: 0 3859 6069 7524 8541 9129
Size: 3859 2210 1455 1017 588
Fragments arranged by size:
3859 2210 1455 1017 588
PasI CC'CwG_GG
Cuts at: 0 469 606 873 1492 2119 3691 4003 4716
Size: 469 137 267 619 627 1572 312 713
Cuts at: 4716 9129
Size: 4413
Fragments arranged by size:
4413 1572 713 627 619 469 312 267
137
PflMI CCAn_nnn'nTGG
Cuts at: 0 708 3765 4771 4948 8260 8745 9129
Size: 708 3057 1006 177 3312 485 384
Fragments arranged by size:
3312 3057 1006 708 485 384 177
PfoI T'CCnGG_A
Cuts at: 0 967 2110 2620 2840 3381 3656 5828 9129
Size: 967 1143 510 220 541 275 2172 3301
Fragments arranged by size:
3301 2172 1143 967 541 510 275 220
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 602 634 9129
Size: 602 32 8495
Fragments arranged by size:
8495 602 32
PpuMI rG'GwC_Cy
Cuts at: 0 712 2035 5009 7572 9084 9129
Size: 712 1323 2974 2563 1512 45
Fragments arranged by size:
2974 2563 1512 1323 712 45
PshAI GACnn'nnGTC
Cuts at: 0 6376 9129
Size: 6376 2753
PspOMI G'GGCC_C
Cuts at: 0 237 3312 3728 4389 8610 8753 9129
Size: 237 3075 416 661 4221 143 376
Fragments arranged by size:
4221 3075 661 416 376 237 143
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 5443 5475 9129
Size: 5443 32 3654
Fragments arranged by size:
5443 3654 32
PstI C_TGCA'G
Cuts at: 0 44 230 487 729 752 851 1707 1727
Size: 44 186 257 242 23 99 856 20
Cuts at: 1727 2300 3608 3986 4535 4604 5123 5306 6225
Size: 573 1308 378 549 69 519 183 919
Cuts at: 6225 6263 6546 6782 8129 8339 8928 9129
Size: 38 283 236 1347 210 589 201
Fragments arranged by size:
1347 1308 919 856 589 573 549 519
378 283 257 242 236 210 201 186
183 99 69 44 38 23 20
PvuI CG_AT'CG
Cuts at: 0 8486 9129
Size: 8486 643
PvuII CAG'CTG
Cuts at: 0 39 180 825 1668 1860 4473 4605 5274
Size: 39 141 645 843 192 2613 132 669
Cuts at: 5274 6541 6724 7026 7378 7579 8334 8422 8547
Size: 1267 183 302 352 201 755 88 125
Cuts at: 8547 9129
Size: 582
Fragments arranged by size:
2613 1267 843 755 669 645 582 352
302 201 192 183 141 132 125 88
39
SacI G_AGCT'C
Cuts at: 0 3665 3718 6308 9129
Size: 3665 53 2590 2821
Fragments arranged by size:
3665 2821 2590 53
SacII CC_GC'GG
Cuts at: 0 734 4201 5194 9129
Size: 734 3467 993 3935
Fragments arranged by size:
3935 3467 993 734
SanDI GG'GwC_CC
Cuts at: 0 712 5009 7572 9084 9129
Size: 712 4297 2563 1512 45
Fragments arranged by size:
4297 2563 1512 712 45
SapI GCTCTTCn'nnn_
Cuts at: 0 2468 3934 4038 6903 9129
Size: 2468 1466 104 2865 2226
Fragments arranged by size:
2865 2468 2226 1466 104
SbfI CC_TGCA'GG
Cuts at: 0 6263 9129
Size: 6263 2866
SexAI A'CCwGG_T
Cuts at: 0 2228 7727 9129
Size: 2228 5499 1402
Fragments arranged by size:
5499 2228 1402
SfcI C'TryA_G
Cuts at: 0 40 226 483 497 725 748 847 1703
Size: 40 186 257 14 228 23 99 856
Cuts at: 1703 1723 2296 3604 3982 4531 4600 4808 5119
Size: 20 573 1308 378 549 69 208 311
Cuts at: 5119 5302 6221 6259 6333 6542 6778 6879 8125
Size: 183 919 38 74 209 236 101 1246
Cuts at: 8125 8335 8924 9129
Size: 210 589 205
Fragments arranged by size:
1308 1246 919 856 589 573 549 378
311 257 236 228 210 209 208 205
186 183 101 99 74 69 40 38
23 20 14
SfoI GGC'GCC
Cuts at: 0 2868 4713 9129
Size: 2868 1845 4416
Fragments arranged by size:
4416 2868 1845
SgrAI Cr'CCGG_yG
Cuts at: 0 4521 9129
Size: 4521 4608
SmaI CCC'GGG
Cuts at: 0 635 909 1674 2515 4641 8317 9129
Size: 635 274 765 841 2126 3676 812
Fragments arranged by size:
3676 2126 841 812 765 635 274
SmlI C'TyrA_G
Cuts at: 0 543 3349 4934 5229 5968 8701 8795 9129
Size: 543 2806 1585 295 739 2733 94 334
Fragments arranged by size:
2806 2733 1585 739 543 334 295 94
SphI G_CATG'C
Cuts at: 0 7524 9129
Size: 7524 1605
SspI AAT'ATT
Cuts at: 0 2541 6663 9129
Size: 2541 4122 2466
Fragments arranged by size:
4122 2541 2466
StuI AGG'CCT
Cuts at: 0 436 989 1449 1903 2599 3934 8259 9129
Size: 436 553 460 454 696 1335 4325 870
Fragments arranged by size:
4325 1335 870 696 553 460 454 436
StyI C'CwwG_G
Cuts at: 0 6 1470 2819 3053 3671 4802 4986 5801
Size: 6 1464 1349 234 618 1131 184 815
Cuts at: 5801 5855 7736 8254 8387 9129
Size: 54 1881 518 133 742
Fragments arranged by size:
1881 1464 1349 1131 815 742 618 518
234 184 133 54 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 610 1505 3114 3589 4040 5952 6414 6429
Size: 610 895 1609 475 451 1912 462 15
Cuts at: 6429 6581 7036 7693 8476 8739 9129
Size: 152 455 657 783 263 390
Fragments arranged by size:
1912 1609 895 783 657 610 475 462
455 451 390 263 152 15
TatI w'GTAC_w
Cuts at: 0 5207 7028 9129
Size: 5207 1821 2101
Fragments arranged by size:
5207 2101 1821
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 269 301 554 586 1608 1640 2335 2367
Size: 269 32 253 32 1022 32 695 32
Cuts at: 2367 2368 2400 2509 2541 4442 4474 4520 4552
Size: 1 32 109 32 1901 32 46 32
Cuts at: 4552 5381 5413 5535 5567 7364 7396 8304 8336
Size: 829 32 122 32 1797 32 908 32
Cuts at: 8336 8501 8533 9129
Size: 165 32 596
Fragments arranged by size:
1901 1797 1022 908 829 695 596 269
253 165 122 109 46 32 32 32
32 32 32 32 32 32 32 32
32 32 1
Tth111I GACn'n_nGTC
Cuts at: 0 4686 5608 6439 9129
Size: 4686 922 831 2690
Fragments arranged by size:
4686 2690 922 831
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 705 8298 9129
Size: 705 7593 831
Fragments arranged by size:
7593 831 705
XmaI C'CCGG_G
Cuts at: 0 633 907 1672 2513 4639 8315 9129
Size: 633 274 765 841 2126 3676 814
Fragments arranged by size:
3676 2126 841 814 765 633 274
ZraI GAC'GTC
Cuts at: 0 941 3069 5392 9129
Size: 941 2128 2323 3737
Fragments arranged by size:
3737 2323 2128 941
Enzymes that do cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AvaI BaeI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI FseI HaeII Hin4I HincII HindIII HpaI KasI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NotI NspI PasI PflMI PfoI
PpiI PpuMI PshAI PspOMI PsrI PstI PvuI PvuII
SacI SacII SanDI SapI SbfI SexAI SfcI SfoI
SgrAI SmaI SmlI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
Acc65I AgeI AscI AseI AsiSI AvrII BamHI BfrBI
BspHI BstBI BstZ17I Bsu36I EcoRI EcoRV FspI FspAI
KpnI NdeI NruI NsiI PacI PciI PmeI PmlI
PsiI PspXI RsrII SalI ScaI SfiI SgfI SnaBI
SpeI SrfI SwaI XbaI XhoI XmnI