Restriction map for
pF1KA1507
(Linear) (Six-base) MAPSORT of: KIAA1507.seq Check: 7938 from: 1 to: 2766
KIAA1507.orf
With 182 enzymes: SgfI *
November 13, 2006 11:21 ..
AatII G_ACGT'C
Cuts at: 0 782 2260 2766
Size: 782 1478 506
Fragments arranged by size:
1478 782 506
AccI GT'mk_AC
Cuts at: 0 1358 2426 2766
Size: 1358 1068 340
Fragments arranged by size:
1358 1068 340
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2029 2172 2766
Size: 2029 143 594
Fragments arranged by size:
2029 594 143
AflIII A'CryG_T
Cuts at: 0 1212 2766
Size: 1212 1554
AhdI GACnn_n'nnGTC
Cuts at: 0 996 2766
Size: 996 1770
AleI CACnn'nnGTG
Cuts at: 0 373 1774 2766
Size: 373 1401 992
Fragments arranged by size:
1401 992 373
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1486 1518 2766
Size: 1486 32 1248
Fragments arranged by size:
1486 1248 32
AlwNI CAG_nnn'CTG
Cuts at: 0 964 2179 2199 2487 2766
Size: 964 1215 20 288 279
Fragments arranged by size:
1215 964 288 279 20
ApaI G_GGCC'C
Cuts at: 0 294 2766
Size: 294 2472
ApoI r'AATT_y
Cuts at: 0 321 911 2766
Size: 321 590 1855
Fragments arranged by size:
1855 590 321
AvaI C'yCGr_G
Cuts at: 0 10 825 884 1608 1629 2147 2766
Size: 10 815 59 724 21 518 619
Fragments arranged by size:
815 724 619 518 59 21 10
AvrII C'CTAG_G
Cuts at: 0 1803 2461 2766
Size: 1803 658 305
Fragments arranged by size:
1803 658 305
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 497 530 2766
Size: 497 33 2236
Fragments arranged by size:
2236 497 33
BamHI G'GATC_C
Cuts at: 0 1262 2490 2766
Size: 1262 1228 276
Fragments arranged by size:
1262 1228 276
BanI G'GyrC_C
Cuts at: 0 750 874 1293 1765 2070 2766
Size: 750 124 419 472 305 696
Fragments arranged by size:
750 696 472 419 305 124
BanII G_rGCy'C
Cuts at: 0 294 1802 2006 2766
Size: 294 1508 204 760
Fragments arranged by size:
1508 760 294 204
BbeI G_GCGC'C
Cuts at: 0 754 2766
Size: 754 2012
BbsI GAAGACnn'nnnn_
Cuts at: 0 734 2766
Size: 734 2032
BbvCI CC'TCA_GC
Cuts at: 0 820 1453 1555 2180 2766
Size: 820 633 102 625 586
Fragments arranged by size:
820 633 625 586 102
BclI T'GATC_A
Cuts at: 0 2330 2766
Size: 2330 436
BfrBI ATG'CAT
Cuts at: 0 2377 2766
Size: 2377 389
BglI GCCn_nnn'nGGC
Cuts at: 0 1791 2766
Size: 1791 975
BlpI GC'TnA_GC
Cuts at: 0 1550 1975 2766
Size: 1550 425 791
Fragments arranged by size:
1550 791 425
Bme1580I G_kGCm'C
Cuts at: 0 294 540 877 1296 1768 2405 2556 2766
Size: 294 246 337 419 472 637 151 210
Fragments arranged by size:
637 472 419 337 294 246 210 151
BmgBI CAC'GTC
Cuts at: 0 384 1776 2022 2766
Size: 384 1392 246 744
Fragments arranged by size:
1392 744 384 246
BmrI ACTGGGnnnn_n'
Cuts at: 0 297 453 1354 2766
Size: 297 156 901 1412
Fragments arranged by size:
1412 901 297 156
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1644 1742 2766
Size: 1644 98 1024
Fragments arranged by size:
1644 1024 98
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 1494 1498 1972 2050 2302 2766
Size: 232 1262 4 474 78 252 464
Fragments arranged by size:
1262 474 464 252 232 78 4
Bpu10I CC'TnA_GC
Cuts at: 0 152 820 1453 1555 1796 2180 2506 2766
Size: 152 668 633 102 241 384 326 260
Fragments arranged by size:
668 633 384 326 260 241 152 102
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1671 2766
Size: 1671 1095
BsaI GGTCTCn'nnnn_
Cuts at: 0 1004 1743 2766
Size: 1004 739 1023
Fragments arranged by size:
1023 1004 739
BsaHI Gr'CG_yC
Cuts at: 0 4 395 751 779 2257 2766
Size: 4 391 356 28 1478 509
Fragments arranged by size:
1478 509 391 356 28 4
BsaWI w'CCGG_w
Cuts at: 0 1394 1728 2766
Size: 1394 334 1038
Fragments arranged by size:
1394 1038 334
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 230 260 1486 1516 2023 2053 2766
Size: 230 30 1226 30 507 30 713
Fragments arranged by size:
1226 713 507 230 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 445 540 1026 1387 1596 2086 2766
Size: 445 95 486 361 209 490 680
Fragments arranged by size:
680 490 486 445 361 209 95
BseYI C'CCAG_C
Cuts at: 0 450 695 800 928 1706 1991 2263 2766
Size: 450 245 105 128 778 285 272 503
Fragments arranged by size:
778 503 450 285 272 245 128 105
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1636 2002 2766
Size: 1636 366 764
Fragments arranged by size:
1636 764 366
BsiHKAI G_wGCw'C
Cuts at: 0 389 1972 2628 2766
Size: 389 1583 656 138
Fragments arranged by size:
1583 656 389 138
BsmI GAATG_Cn'
Cuts at: 0 15 1586 2766
Size: 15 1571 1180
Fragments arranged by size:
1571 1180 15
BsmBI CGTCTCn'nnnn_
Cuts at: 0 54 214 1726 2028 2751 2766
Size: 54 160 1512 302 723 15
Fragments arranged by size:
1512 723 302 160 54 15
Bsp1286I G_dGCh'C
Cuts at: 0 294 389 540 877 1296 1768 1802 1972
Size: 294 95 151 337 419 472 34 170
Cuts at: 1972 2006 2405 2556 2628 2766
Size: 34 399 151 72 138
Fragments arranged by size:
472 419 399 337 294 170 151 151
138 95 72 34 34
BspEI T'CCGG_A
Cuts at: 0 1728 2766
Size: 1728 1038
BspHI T'CATG_A
Cuts at: 0 2439 2766
Size: 2439 327
BsrBI CCG'CTC
Cuts at: 0 1788 2766
Size: 1788 978
BsrDI GCAATG_nn'
Cuts at: 0 77 2325 2766
Size: 77 2248 441
Fragments arranged by size:
2248 441 77
BsrFI r'CCGG_y
Cuts at: 0 1881 2766
Size: 1881 885
BssSI C'ACGA_G
Cuts at: 0 616 2766
Size: 616 2150
BstBI TT'CG_AA
Cuts at: 0 1578 2766
Size: 1578 1188
BstEII G'GTnAC_C
Cuts at: 0 2500 2766
Size: 2500 266
BstXI CCAn_nnnn'nTGG
Cuts at: 0 405 1776 2615 2766
Size: 405 1371 839 151
Fragments arranged by size:
1371 839 405 151
BstYI r'GATC_y
Cuts at: 0 1262 2490 2766
Size: 1262 1228 276
Fragments arranged by size:
1262 1228 276
BstZ17I GTA'TAC
Cuts at: 0 2427 2766
Size: 2427 339
Bsu36I CC'TnA_GG
Cuts at: 0 296 328 1672 2766
Size: 296 32 1344 1094
Fragments arranged by size:
1344 1094 296 32
BtgI C'CryG_G
Cuts at: 0 1143 1523 2633 2766
Size: 1143 380 1110 133
Fragments arranged by size:
1143 1110 380 133
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2555 2766
Size: 2555 211
BtsI GCAGTG_nn'
Cuts at: 0 844 2648 2766
Size: 844 1804 118
Fragments arranged by size:
1804 844 118
ClaI AT'CG_AT
Cuts at: 0 1961 2766
Size: 1961 805
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 602 637 2030 2065 2766
Size: 602 35 1393 35 701
Fragments arranged by size:
1393 701 602 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 2500 2766
Size: 2500 266
DrdI GACnn_nn'nnGTC
Cuts at: 0 690 736 2766
Size: 690 46 2030
Fragments arranged by size:
2030 690 46
EaeI y'GGCC_r
Cuts at: 0 74 1593 2294 2636 2766
Size: 74 1519 701 342 130
Fragments arranged by size:
1519 701 342 130 74
EarI CTCTTCn'nnn_
Cuts at: 0 422 1252 2128 2674 2766
Size: 422 830 876 546 92
Fragments arranged by size:
876 830 546 422 92
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 1494 1498 1972 2029 2050 2172 2302
Size: 232 1262 4 474 57 21 122 130
Cuts at: 2302 2766
Size: 464
Fragments arranged by size:
1262 474 464 232 130 122 57 21
4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1222 1440 2766
Size: 1222 218 1326
Fragments arranged by size:
1326 1222 218
EcoO109I rG'GnC_Cy
Cuts at: 0 291 342 447 861 1467 1689 2153 2196
Size: 291 51 105 414 606 222 464 43
Cuts at: 2196 2249 2766
Size: 53 517
Fragments arranged by size:
606 517 464 414 291 222 105 53
51 43
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1683 2766
Size: 1683 1083
FspI TGC'GCA
Cuts at: 0 772 2766
Size: 772 1994
FspAI rTGC'GCAy
Cuts at: 0 772 2766
Size: 772 1994
HaeII r_GCGC'y
Cuts at: 0 754 2766
Size: 754 2012
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 977 982 1009 1014 1591 1623 2023 2055
Size: 977 5 27 5 577 32 400 32
Cuts at: 2055 2127 2159 2675 2707 2766
Size: 72 32 516 32 59
Fragments arranged by size:
977 577 516 400 72 59 32 32
32 32 27 5 5
HincII GTy'rAC
Cuts at: 0 1109 1359 2060 2766
Size: 1109 250 701 706
Fragments arranged by size:
1109 706 701 250
KasI G'GCGC_C
Cuts at: 0 750 2766
Size: 750 2016
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 2049 2766
Size: 193 1856 717
Fragments arranged by size:
1856 717 193
MscI TGG'CCA
Cuts at: 0 76 2296 2766
Size: 76 2220 470
Fragments arranged by size:
2220 470 76
MslI CAynn'nnrTG
Cuts at: 0 373 1211 1774 2173 2218 2766
Size: 373 838 563 399 45 548
Fragments arranged by size:
838 563 548 399 373 45
MspA1I CmG'CkG
Cuts at: 0 70 454 932 1042 1991 2263 2766
Size: 70 384 478 110 949 272 503
Fragments arranged by size:
949 503 478 384 272 110 70
NaeI GCC'GGC
Cuts at: 0 1883 2766
Size: 1883 883
NarI GG'CG_CC
Cuts at: 0 751 2766
Size: 751 2015
NcoI C'CATG_G
Cuts at: 0 2633 2766
Size: 2633 133
NgoMIV G'CCGG_C
Cuts at: 0 1881 2766
Size: 1881 885
NsiI A_TGCA'T
Cuts at: 0 2379 2766
Size: 2379 387
NspI r_CATG'y
Cuts at: 0 1216 1983 2377 2409 2766
Size: 1216 767 394 32 357
Fragments arranged by size:
1216 767 394 357 32
PciI A'CATG_T
Cuts at: 0 1212 2766
Size: 1212 1554
PflMI CCAn_nnn'nTGG
Cuts at: 0 2199 2217 2766
Size: 2199 18 549
Fragments arranged by size:
2199 549 18
PfoI T'CCnGG_A
Cuts at: 0 248 722 2486 2766
Size: 248 474 1764 280
Fragments arranged by size:
1764 474 280 248
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1486 1518 2766
Size: 1486 32 1248
Fragments arranged by size:
1486 1248 32
PpuMI rG'GwC_Cy
Cuts at: 0 447 861 1467 2196 2766
Size: 447 414 606 729 570
Fragments arranged by size:
729 606 570 447 414
PspOMI G'GGCC_C
Cuts at: 0 290 2766
Size: 290 2476
PspXI vC'TCGA_Gb
Cuts at: 0 2147 2766
Size: 2147 619
PstI C_TGCA'G
Cuts at: 0 263 1013 1303 1463 2520 2766
Size: 263 750 290 160 1057 246
Fragments arranged by size:
1057 750 290 263 246 160
PvuII CAG'CTG
Cuts at: 0 70 454 2263 2766
Size: 70 384 1809 503
Fragments arranged by size:
1809 503 384 70
SalI G'TCGA_C
Cuts at: 0 1357 2766
Size: 1357 1409
SanDI GG'GwC_CC
Cuts at: 0 447 1467 2766
Size: 447 1020 1299
Fragments arranged by size:
1299 1020 447
SapI GCTCTTCn'nnn_
Cuts at: 0 2128 2766
Size: 2128 638
SbfI CC_TGCA'GG
Cuts at: 0 1303 2766
Size: 1303 1463
ScaI AGT'ACT
Cuts at: 0 1666 2766
Size: 1666 1100
SexAI A'CCwGG_T
Cuts at: 0 227 2496 2766
Size: 227 2269 270
Fragments arranged by size:
2269 270 227
SfcI C'TryA_G
Cuts at: 0 259 1009 1299 1437 1459 2516 2655 2766
Size: 259 750 290 138 22 1057 139 111
Fragments arranged by size:
1057 750 290 259 139 138 111 22
SfoI GGC'GCC
Cuts at: 0 752 2766
Size: 752 2014
SmaI CCC'GGG
Cuts at: 0 12 827 886 2766
Size: 12 815 59 1880
Fragments arranged by size:
1880 815 59 12
SmlI C'TyrA_G
Cuts at: 0 1650 2147 2766
Size: 1650 497 619
Fragments arranged by size:
1650 619 497
SphI G_CATG'C
Cuts at: 0 1983 2377 2766
Size: 1983 394 389
Fragments arranged by size:
1983 394 389
SrfI GCCC'GGGC
Cuts at: 0 12 827 2766
Size: 12 815 1939
Fragments arranged by size:
1939 815 12
StuI AGG'CCT
Cuts at: 0 402 2564 2612 2766
Size: 402 2162 48 154
Fragments arranged by size:
2162 402 154 48
StyI C'CwwG_G
Cuts at: 0 703 1803 2291 2348 2461 2633 2766
Size: 703 1100 488 57 113 172 133
Fragments arranged by size:
1100 703 488 172 133 113 57
TatI w'GTAC_w
Cuts at: 0 1091 1664 2766
Size: 1091 573 1102
Fragments arranged by size:
1102 1091 573
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 84 375 2304 2766
Size: 84 291 1929 462
Fragments arranged by size:
1929 462 291 84
XhoI C'TCGA_G
Cuts at: 0 2147 2766
Size: 2147 619
XmaI C'CCGG_G
Cuts at: 0 10 825 884 2766
Size: 10 815 59 1882
Fragments arranged by size:
1882 815 59 10
XmnI GAAnn'nnTTC
Cuts at: 0 1501 2766
Size: 1501 1265
ZraI GAC'GTC
Cuts at: 0 780 2258 2766
Size: 780 1478 508
Fragments arranged by size:
1478 780 508
Enzymes that do cut:
AatII AccI AcuI AflIII AhdI AleI AloI AlwNI
ApaI ApoI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BbvCI BclI BfrBI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BsrBI BsrDI BsrFI BssSI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraIII DrdI EaeI EarI Eco57MI EcoNI
EcoO109I FalI FspI FspAI HaeII Hin4I HincII KasI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PflMI PfoI PpiI PpuMI PspOMI
PspXI PstI PvuII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SmaI SmlI SphI SrfI StuI
StyI TatI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AlfI ApaLI
AscI AseI AsiSI BcgI BciVI BglII BmtI BsaAI
BsaBI BsiEI BsiWI BspMI BsrGI BssHII BstAPI DraI
EagI EciI EcoICRI EcoRI EcoRV FseI HindIII HpaI
KpnI MfeI MluI NdeI NheI NotI NruI PacI
PasI PmeI PmlI PshAI PsiI PsrI PvuI RsrII
SacI SacII SfiI SgfI SgrAI SnaBI SpeI SspI
SwaI TaqII TstI Tth111I XbaI