Restriction map for
pF1KSDA1519
(Linear) (Six-base) MAPSORT of: KIAA1519.seq Check: 1490 from: 1 to: 1941
KIAA1519.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 386 1811 1941
Size: 386 1425 130
Fragments arranged by size:
1425 386 130
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 633 1732 1941
Size: 73 560 1099 209
Fragments arranged by size:
1099 560 209 73
AflIII A'CryG_T
Cuts at: 0 881 1941
Size: 881 1060
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1333 1941
Size: 1333 608
AlwNI CAG_nnn'CTG
Cuts at: 0 222 399 561 1298 1636 1722 1761 1815
Size: 222 177 162 737 338 86 39 54
Cuts at: 1815 1941
Size: 126
Fragments arranged by size:
737 338 222 177 162 126 86 54
39
ApaLI G'TGCA_C
Cuts at: 0 373 534 1941
Size: 373 161 1407
Fragments arranged by size:
1407 373 161
ApoI r'AATT_y
Cuts at: 0 1375 1650 1941
Size: 1375 275 291
Fragments arranged by size:
1375 291 275
AvaI C'yCGr_G
Cuts at: 0 634 1181 1213 1234 1487 1941
Size: 634 547 32 21 253 454
Fragments arranged by size:
634 547 454 253 32 21
BanI G'GyrC_C
Cuts at: 0 963 1941
Size: 963 978
BanII G_rGCy'C
Cuts at: 0 308 712 1751 1880 1941
Size: 308 404 1039 129 61
Fragments arranged by size:
1039 404 308 129 61
BbsI GAAGACnn'nnnn_
Cuts at: 0 1249 1891 1941
Size: 1249 642 50
Fragments arranged by size:
1249 642 50
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1162 1196 1941
Size: 1162 34 745
Fragments arranged by size:
1162 745 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 906 1941
Size: 906 1035
BglI GCCn_nnn'nGGC
Cuts at: 0 1255 1925 1941
Size: 1255 670 16
Fragments arranged by size:
1255 670 16
BlpI GC'TnA_GC
Cuts at: 0 28 83 1466 1816 1941
Size: 28 55 1383 350 125
Fragments arranged by size:
1383 350 125 55 28
Bme1580I G_kGCm'C
Cuts at: 0 377 538 870 1088 1941
Size: 377 161 332 218 853
Fragments arranged by size:
853 377 332 218 161
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 690 1941
Size: 690 1251
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 376 814 1288 1456 1744 1884 1941
Size: 226 150 438 474 168 288 140 57
Fragments arranged by size:
474 438 288 226 168 150 140 57
Bpu10I CC'TnA_GC
Cuts at: 0 60 1099 1343 1388 1941
Size: 60 1039 244 45 553
Fragments arranged by size:
1039 553 244 60 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1864 1941
Size: 1864 77
BsaI GGTCTCn'nnnn_
Cuts at: 0 1184 1941
Size: 1184 757
BsaAI yAC'GTr
Cuts at: 0 884 1941
Size: 884 1057
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 247 277 889 919 947 977 1162 1192
Size: 247 30 612 30 28 30 185 30
Cuts at: 1192 1429 1459 1941
Size: 237 30 482
Fragments arranged by size:
612 482 247 237 185 30 30 30
30 30 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 49 255 780 783 1183 1941
Size: 49 206 525 3 400 758
Fragments arranged by size:
758 525 400 206 49 3
BseYI C'CCAG_C
Cuts at: 0 489 1298 1941
Size: 489 809 643
Fragments arranged by size:
809 643 489
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1014 1303 1501 1649 1941
Size: 1014 289 198 148 292
Fragments arranged by size:
1014 292 289 198 148
BsiEI CG_ry'CG
Cuts at: 0 408 1941
Size: 408 1533
BsiHKAI G_wGCw'C
Cuts at: 0 308 377 538 643 1730 1751 1880 1941
Size: 308 69 161 105 1087 21 129 61
Fragments arranged by size:
1087 308 161 129 105 69 61 21
BsmI GAATG_Cn'
Cuts at: 0 212 1013 1333 1941
Size: 212 801 320 608
Fragments arranged by size:
801 608 320 212
BsmBI CGTCTCn'nnnn_
Cuts at: 0 754 1214 1264 1941
Size: 754 460 50 677
Fragments arranged by size:
754 677 460 50
Bsp1286I G_dGCh'C
Cuts at: 0 308 377 538 643 712 870 1088 1730
Size: 308 69 161 105 69 158 218 642
Cuts at: 1730 1751 1880 1941
Size: 21 129 61
Fragments arranged by size:
642 308 218 161 158 129 105 69
69 61 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 386 1301 1811 1941
Size: 386 915 510 130
Fragments arranged by size:
915 510 386 130
BsrBI CCG'CTC
Cuts at: 0 56 429 847 1941
Size: 56 373 418 1094
Fragments arranged by size:
1094 418 373 56
BsrDI GCAATG_nn'
Cuts at: 0 599 1941
Size: 599 1342
BssSI C'ACGA_G
Cuts at: 0 307 642 677 1941
Size: 307 335 35 1264
Fragments arranged by size:
1264 335 307 35
BstAPI GCAn_nnn'nTGC
Cuts at: 0 785 1339 1636 1761 1941
Size: 785 554 297 125 180
Fragments arranged by size:
785 554 297 180 125
BstXI CCAn_nnnn'nTGG
Cuts at: 0 820 1941
Size: 820 1121
BstYI r'GATC_y
Cuts at: 0 177 1739 1941
Size: 177 1562 202
Fragments arranged by size:
1562 202 177
Bsu36I CC'TnA_GG
Cuts at: 0 1151 1941
Size: 1151 790
BtgI C'CryG_G
Cuts at: 0 335 908 1889 1920 1941
Size: 335 573 981 31 21
Fragments arranged by size:
981 573 335 31 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1417 1941
Size: 1417 524
BtsI GCAGTG_nn'
Cuts at: 0 1634 1941
Size: 1634 307
DrdI GACnn_nn'nnGTC
Cuts at: 0 586 1941
Size: 586 1355
EaeI y'GGCC_r
Cuts at: 0 548 1941
Size: 548 1393
EarI CTCTTCn'nnn_
Cuts at: 0 46 936 1568 1618 1678 1941
Size: 46 890 632 50 60 263
Fragments arranged by size:
890 632 263 60 50 46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 821 1525 1941
Size: 821 704 416
Fragments arranged by size:
821 704 416
EcoICRI GAG'CTC
Cuts at: 0 306 1749 1878 1941
Size: 306 1443 129 63
Fragments arranged by size:
1443 306 129 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 226 376 633 814 1288 1456 1732
Size: 73 153 150 257 181 474 168 276
Cuts at: 1732 1744 1884 1941
Size: 12 140 57
Fragments arranged by size:
474 276 257 181 168 153 150 140
73 57 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1342 1807 1941
Size: 1342 465 134
Fragments arranged by size:
1342 465 134
EcoO109I rG'GnC_Cy
Cuts at: 0 64 618 1076 1136 1313 1941
Size: 64 554 458 60 177 628
Fragments arranged by size:
628 554 458 177 64 60
EcoRI G'AATT_C
Cuts at: 0 1375 1941
Size: 1375 566
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 75 1941
Size: 75 1866
HaeII r_GCGC'y
Cuts at: 0 1264 1916 1941
Size: 1264 652 25
Fragments arranged by size:
1264 652 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 76 108 228 260 913 945 1128 1160
Size: 76 32 120 32 653 32 183 32
Cuts at: 1160 1162 1194 1408 1429 1440 1461 1941
Size: 2 32 214 21 11 21 480
Fragments arranged by size:
653 480 214 183 120 76 32 32
32 32 32 21 21 11 2
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 923 996 1941
Size: 923 73 945
Fragments arranged by size:
945 923 73
MscI TGG'CCA
Cuts at: 0 550 1941
Size: 550 1391
MslI CAynn'nnrTG
Cuts at: 0 1756 1941
Size: 1756 185
MspA1I CmG'CkG
Cuts at: 0 399 776 1941
Size: 399 377 1165
Fragments arranged by size:
1165 399 377
NcoI C'CATG_G
Cuts at: 0 908 1889 1920 1941
Size: 908 981 31 21
Fragments arranged by size:
981 908 31 21
PflMI CCAn_nnn'nTGG
Cuts at: 0 399 1941
Size: 399 1542
PfoI T'CCnGG_A
Cuts at: 0 105 1265 1742 1941
Size: 105 1160 477 199
Fragments arranged by size:
1160 477 199 105
PpuMI rG'GwC_Cy
Cuts at: 0 618 1076 1136 1313 1941
Size: 618 458 60 177 628
Fragments arranged by size:
628 618 458 177 60
PstI C_TGCA'G
Cuts at: 0 781 1256 1312 1641 1766 1811 1941
Size: 781 475 56 329 125 45 130
Fragments arranged by size:
781 475 329 130 125 56 45
PvuI CG_AT'CG
Cuts at: 0 408 1941
Size: 408 1533
PvuII CAG'CTG
Cuts at: 0 399 776 1941
Size: 399 377 1165
Fragments arranged by size:
1165 399 377
SacI G_AGCT'C
Cuts at: 0 308 1751 1880 1941
Size: 308 1443 129 61
Fragments arranged by size:
1443 308 129 61
SapI GCTCTTCn'nnn_
Cuts at: 0 46 1941
Size: 46 1895
SbfI CC_TGCA'GG
Cuts at: 0 1256 1941
Size: 1256 685
SexAI A'CCwGG_T
Cuts at: 0 1283 1941
Size: 1283 658
SfcI C'TryA_G
Cuts at: 0 777 1252 1308 1637 1762 1807 1941
Size: 777 475 56 329 125 45 134
Fragments arranged by size:
777 475 329 134 125 56 45
SmaI CCC'GGG
Cuts at: 0 636 1215 1236 1489 1941
Size: 636 579 21 253 452
Fragments arranged by size:
636 579 452 253 21
SmlI C'TyrA_G
Cuts at: 0 1879 1941
Size: 1879 62
StuI AGG'CCT
Cuts at: 0 544 630 859 1156 1936 1941
Size: 544 86 229 297 780 5
Fragments arranged by size:
780 544 297 229 86 5
StyI C'CwwG_G
Cuts at: 0 194 545 805 908 1088 1889 1920 1941
Size: 194 351 260 103 180 801 31 21
Fragments arranged by size:
801 351 260 194 180 103 31 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 145 914 1207 1399 1941
Size: 145 769 293 192 542
Fragments arranged by size:
769 542 293 192 145
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 889 921 947 979 1941
Size: 889 32 26 32 962
Fragments arranged by size:
962 889 32 32 26
Tth111I GACn'n_nGTC
Cuts at: 0 1140 1941
Size: 1140 801
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 558 1941
Size: 558 1383
XmaI C'CCGG_G
Cuts at: 0 634 1213 1234 1487 1941
Size: 634 579 21 253 454
Fragments arranged by size:
634 579 454 253 21
XmnI GAAnn'nnTTC
Cuts at: 0 281 1941
Size: 281 1660
Enzymes that do cut:
AarI AcuI AflIII AlfI AlwNI ApaLI ApoI AvaI
BanI BanII BbsI BcgI BciVI BglI BlpI Bme1580I
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrBI BsrDI BssSI BstAPI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DrdI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I MmeI MscI
MslI MspA1I NcoI PflMI PfoI PpuMI PstI PvuI
PvuII SacI SapI SbfI SexAI SfcI SmaI SmlI
StuI StyI TaqII TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BclI BfrBI BglII BmgBI BmrI
BmtI BsaBI BsaHI BsaWI BsiWI BspEI BspHI BsrFI
BsrGI BssHII BstBI BstEII BstZ17I ClaI CspCI DraI
DraIII EagI EcoRV FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI RsrII SacII SalI SanDI ScaI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
TatI XbaI XhoI ZraI