Restriction map for
pF1KA1520
(Linear) (Six-base) MAPSORT of: KIAA1520.seq Check: 7260 from: 1 to: 2298
KIAA1520.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1179 2298
Size: 1179 1119
AccI GT'mk_AC
Cuts at: 0 1394 2298
Size: 1394 904
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 1563 2218 2298
Size: 63 1500 655 80
Fragments arranged by size:
1500 655 80 63
AfeI AGC'GCT
Cuts at: 0 2013 2298
Size: 2013 285
AflIII A'CryG_T
Cuts at: 0 1226 2298
Size: 1226 1072
AleI CACnn'nnGTG
Cuts at: 0 262 946 2298
Size: 262 684 1352
Fragments arranged by size:
1352 684 262
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 193 2298
Size: 193 2105
AlwNI CAG_nnn'CTG
Cuts at: 0 438 528 1563 2178 2181 2226 2298
Size: 438 90 1035 615 3 45 72
Fragments arranged by size:
1035 615 438 90 72 45 3
ApaI G_GGCC'C
Cuts at: 0 227 354 1619 2298
Size: 227 127 1265 679
Fragments arranged by size:
1265 679 227 127
ApaLI G'TGCA_C
Cuts at: 0 1468 2298
Size: 1468 830
AvaI C'yCGr_G
Cuts at: 0 496 1620 1961 2298
Size: 496 1124 341 337
Fragments arranged by size:
1124 496 341 337
BanI G'GyrC_C
Cuts at: 0 427 1918 2164 2298
Size: 427 1491 246 134
Fragments arranged by size:
1491 427 246 134
BanII G_rGCy'C
Cuts at: 0 227 354 1253 1390 1619 1639 1766 1968
Size: 227 127 899 137 229 20 127 202
Cuts at: 1968 2298
Size: 330
Fragments arranged by size:
899 330 229 227 202 137 127 127
20
BbeI G_GCGC'C
Cuts at: 0 1922 2298
Size: 1922 376
BbsI GAAGACnn'nnnn_
Cuts at: 0 69 909 948 1132 2298
Size: 69 840 39 184 1166
Fragments arranged by size:
1166 840 184 69 39
BbvCI CC'TCA_GC
Cuts at: 0 464 2298
Size: 464 1834
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 666 700 1317 1351 1449 1483 2298
Size: 666 34 617 34 98 34 815
Fragments arranged by size:
815 666 617 98 34 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 231 460 2298
Size: 231 229 1838
Fragments arranged by size:
1838 231 229
BlpI GC'TnA_GC
Cuts at: 0 2101 2298
Size: 2101 197
Bme1580I G_kGCm'C
Cuts at: 0 227 354 1472 1619 2167 2298
Size: 227 127 1118 147 548 131
Fragments arranged by size:
1118 548 227 147 131 127
BmgBI CAC'GTC
Cuts at: 0 552 2298
Size: 552 1746
BmrI ACTGGGnnnn_n'
Cuts at: 0 1978 2298
Size: 1978 320
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 460 1519 1675 1693 1867 2298
Size: 124 336 1059 156 18 174 431
Fragments arranged by size:
1059 431 336 174 156 124 18
Bpu10I CC'TnA_GC
Cuts at: 0 464 2298
Size: 464 1834
BsaI GGTCTCn'nnnn_
Cuts at: 0 598 903 1102 2298
Size: 598 305 199 1196
Fragments arranged by size:
1196 598 305 199
BsaHI Gr'CG_yC
Cuts at: 0 505 1919 2298
Size: 505 1414 379
Fragments arranged by size:
1414 505 379
BsaWI w'CCGG_w
Cuts at: 0 1133 2298
Size: 1133 1165
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 841 871 883 913 2298
Size: 841 30 12 30 1385
Fragments arranged by size:
1385 841 30 30 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1120 1165 1168 1171 2298
Size: 1120 45 3 3 1127
Fragments arranged by size:
1127 1120 45 3 3
BseYI C'CCAG_C
Cuts at: 0 183 219 1922 2226 2298
Size: 183 36 1703 304 72
Fragments arranged by size:
1703 304 183 72 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2176 2233 2298
Size: 2176 57 65
Fragments arranged by size:
2176 65 57
BsiEI CG_ry'CG
Cuts at: 0 486 1452 2298
Size: 486 966 846
Fragments arranged by size:
966 846 486
BsiHKAI G_wGCw'C
Cuts at: 0 1472 1639 2107 2117 2298
Size: 1472 167 468 10 181
Fragments arranged by size:
1472 468 181 167 10
BsmI GAATG_Cn'
Cuts at: 0 1080 2298
Size: 1080 1218
Bsp1286I G_dGCh'C
Cuts at: 0 227 354 1253 1390 1472 1619 1639 1766
Size: 227 127 899 137 82 147 20 127
Cuts at: 1766 1968 2107 2117 2167 2298
Size: 202 139 10 50 131
Fragments arranged by size:
899 227 202 147 139 137 131 127
127 82 50 20 10
BspEI T'CCGG_A
Cuts at: 0 1133 2298
Size: 1133 1165
BspHI T'CATG_A
Cuts at: 0 38 1016 2298
Size: 38 978 1282
Fragments arranged by size:
1282 978 38
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 718 1179 1181 2072 2298
Size: 718 461 2 891 226
Fragments arranged by size:
891 718 461 226 2
BsrBI CCG'CTC
Cuts at: 0 482 800 1781 2298
Size: 482 318 981 517
Fragments arranged by size:
981 517 482 318
BsrFI r'CCGG_y
Cuts at: 0 485 1451 2096 2298
Size: 485 966 645 202
Fragments arranged by size:
966 645 485 202
BsrGI T'GTAC_A
Cuts at: 0 1193 2298
Size: 1193 1105
BssSI C'ACGA_G
Cuts at: 0 243 262 562 2298
Size: 243 19 300 1736
Fragments arranged by size:
1736 300 243 19
BstAPI GCAn_nnn'nTGC
Cuts at: 0 528 2298
Size: 528 1770
BstBI TT'CG_AA
Cuts at: 0 783 2298
Size: 783 1515
BstEII G'GTnAC_C
Cuts at: 0 990 2298
Size: 990 1308
BstXI CCAn_nnnn'nTGG
Cuts at: 0 201 2016 2298
Size: 201 1815 282
Fragments arranged by size:
1815 282 201
BstYI r'GATC_y
Cuts at: 0 144 2298
Size: 144 2154
BtgI C'CryG_G
Cuts at: 0 62 290 881 1382 1463 1863 1955 2055
Size: 62 228 591 501 81 400 92 100
Cuts at: 2055 2108 2298
Size: 53 190
Fragments arranged by size:
591 501 400 228 190 100 92 81
62 53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1320 2073 2298
Size: 1320 753 225
Fragments arranged by size:
1320 753 225
BtsI GCAGTG_nn'
Cuts at: 0 1255 1632 2298
Size: 1255 377 666
Fragments arranged by size:
1255 666 377
DraIII CAC_nnn'GTG
Cuts at: 0 1740 2298
Size: 1740 558
EaeI y'GGCC_r
Cuts at: 0 65 203 421 452 483 487 566 704
Size: 65 138 218 31 31 4 79 138
Cuts at: 704 1082 1935 1952 2259 2298
Size: 378 853 17 307 39
Fragments arranged by size:
853 378 307 218 138 138 79 65
39 31 31 17 4
EagI C'GGCC_G
Cuts at: 0 483 2298
Size: 483 1815
EarI CTCTTCn'nnn_
Cuts at: 0 277 313 799 1627 2298
Size: 277 36 486 828 671
Fragments arranged by size:
828 671 486 277 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 103 1117 2298
Size: 103 1014 1181
Fragments arranged by size:
1181 1014 103
EcoICRI GAG'CTC
Cuts at: 0 1637 2298
Size: 1637 661
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 124 460 1519 1563 1675 1693 1867
Size: 63 61 336 1059 44 112 18 174
Cuts at: 1867 2218 2298
Size: 351 80
Fragments arranged by size:
1059 351 336 174 112 80 63 61
44 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 103 1408 2298
Size: 103 1305 890
Fragments arranged by size:
1305 890 103
EcoO109I rG'GnC_Cy
Cuts at: 0 223 224 336 351 435 849 1560 1615
Size: 223 1 112 15 84 414 711 55
Cuts at: 1615 1616 2298
Size: 1 682
Fragments arranged by size:
711 682 414 223 112 84 55 15
1 1
FseI GG_CCGG'CC
Cuts at: 0 489 2298
Size: 489 1809
FspI TGC'GCA
Cuts at: 0 322 2298
Size: 322 1976
HaeII r_GCGC'y
Cuts at: 0 1715 1922 2015 2298
Size: 1715 207 93 283
Fragments arranged by size:
1715 283 207 93
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 490 522 841 873 883 915 1783 1815
Size: 490 32 319 32 10 32 868 32
Cuts at: 1815 2019 2051 2298
Size: 204 32 247
Fragments arranged by size:
868 490 319 247 204 32 32 32
32 32 10
HincII GTy'rAC
Cuts at: 0 1647 2298
Size: 1647 651
HindIII A'AGCT_T
Cuts at: 0 822 2298
Size: 822 1476
KasI G'GCGC_C
Cuts at: 0 1918 2298
Size: 1918 380
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1052 2298
Size: 1052 1246
MscI TGG'CCA
Cuts at: 0 205 706 1084 1937 1954 2261 2298
Size: 205 501 378 853 17 307 37
Fragments arranged by size:
853 501 378 307 205 37 17
MslI CAynn'nnrTG
Cuts at: 0 43 55 199 262 880 946 997 1015
Size: 43 12 144 63 618 66 51 18
Cuts at: 1015 1018 1021 2131 2298
Size: 3 3 1110 167
Fragments arranged by size:
1110 618 167 144 66 63 51 43
18 12 3 3
MspA1I CmG'CkG
Cuts at: 0 173 1219 1247 1325 1926 1947 2178 2217
Size: 173 1046 28 78 601 21 231 39
Cuts at: 2217 2257 2298
Size: 40 41
Fragments arranged by size:
1046 601 231 173 78 41 40 39
28 21
NaeI GCC'GGC
Cuts at: 0 487 2298
Size: 487 1811
NarI GG'CG_CC
Cuts at: 0 1919 2298
Size: 1919 379
NcoI C'CATG_G
Cuts at: 0 290 881 1463 1955 2055 2298
Size: 290 591 582 492 100 243
Fragments arranged by size:
591 582 492 290 243 100
NgoMIV G'CCGG_C
Cuts at: 0 485 2298
Size: 485 1813
NspI r_CATG'y
Cuts at: 0 1230 2298
Size: 1230 1068
PasI CC'CwG_GG
Cuts at: 0 475 2187 2298
Size: 475 1712 111
Fragments arranged by size:
1712 475 111
PciI A'CATG_T
Cuts at: 0 1226 2298
Size: 1226 1072
PflMI CCAn_nnn'nTGG
Cuts at: 0 438 2298
Size: 438 1860
PfoI T'CCnGG_A
Cuts at: 0 101 332 1652 1883 1994 2298
Size: 101 231 1320 231 111 304
Fragments arranged by size:
1320 304 231 231 111 101
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1420 1452 2298
Size: 1420 32 846
Fragments arranged by size:
1420 846 32
PpuMI rG'GwC_Cy
Cuts at: 0 336 849 1560 2298
Size: 336 513 711 738
Fragments arranged by size:
738 711 513 336
PspOMI G'GGCC_C
Cuts at: 0 223 350 1615 2298
Size: 223 127 1265 683
Fragments arranged by size:
1265 683 223 127
PstI C_TGCA'G
Cuts at: 0 524 1187 1568 2069 2298
Size: 524 663 381 501 229
Fragments arranged by size:
663 524 501 381 229
PvuII CAG'CTG
Cuts at: 0 173 1219 1926 2178 2257 2298
Size: 173 1046 707 252 79 41
Fragments arranged by size:
1046 707 252 173 79 41
SacI G_AGCT'C
Cuts at: 0 1639 2298
Size: 1639 659
SanDI GG'GwC_CC
Cuts at: 0 336 2298
Size: 336 1962
SapI GCTCTTCn'nnn_
Cuts at: 0 313 1627 2298
Size: 313 1314 671
Fragments arranged by size:
1314 671 313
ScaI AGT'ACT
Cuts at: 0 1045 1729 2298
Size: 1045 684 569
Fragments arranged by size:
1045 684 569
SexAI A'CCwGG_T
Cuts at: 0 893 2298
Size: 893 1405
SfcI C'TryA_G
Cuts at: 0 520 1183 1395 1564 1893 2065 2273 2298
Size: 520 663 212 169 329 172 208 25
Fragments arranged by size:
663 520 329 212 208 172 169 25
SfoI GGC'GCC
Cuts at: 0 1920 2298
Size: 1920 378
SmaI CCC'GGG
Cuts at: 0 1963 2298
Size: 1963 335
SrfI GCCC'GGGC
Cuts at: 0 1963 2298
Size: 1963 335
StyI C'CwwG_G
Cuts at: 0 290 881 986 1463 1955 2055 2298
Size: 290 591 105 477 492 100 243
Fragments arranged by size:
591 492 477 290 243 105 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 445 1570 2182 2298
Size: 445 1125 612 116
Fragments arranged by size:
1125 612 445 116
TatI w'GTAC_w
Cuts at: 0 1043 1193 1229 1727 2298
Size: 1043 150 36 498 571
Fragments arranged by size:
1043 571 498 150 36
Tth111I GACn'n_nGTC
Cuts at: 0 895 2298
Size: 895 1403
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 198 549 1017 1930 2013 2298
Size: 198 351 468 913 83 285
Fragments arranged by size:
913 468 351 285 198 83
XmaI C'CCGG_G
Cuts at: 0 1961 2298
Size: 1961 337
XmnI GAAnn'nnTTC
Cuts at: 0 1435 2298
Size: 1435 863
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AleI AlfI AlwNI
ApaI ApaLI AvaI BanI BanII BbeI BbsI BbvCI
BcgI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FseI FspI HaeII Hin4I HincII
HindIII KasI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PpiI
PpuMI PspOMI PstI PvuII SacI SanDI SapI ScaI
SexAI SfcI SfoI SmaI SrfI StyI TaqII TatI
Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AloI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BciVI BclI
BfrBI BglII BmtI BplI BpuEI BsaAI BsaBI BsiWI
BsmBI BsrDI BssHII BstZ17I Bsu36I ClaI CspCI DraI
DrdI EcoRI EcoRV FalI FspAI HpaI KpnI MfeI
MluI NdeI NheI NotI NruI NsiI PacI PmeI
PmlI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SbfI SfiI SgfI SgrAI SmlI SnaBI SpeI
SphI SspI StuI SwaI TstI XbaI XhoI ZraI