Restriction map for
pF1KA1521
(Linear) (Six-base) MAPSORT of: KIAA1521.seq Check: 9640 from: 1 to: 4434
KIAA1521.orf
With 182 enzymes: SgfI *
November 13, 2006 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2020 4434
Size: 2020 2414
AatII G_ACGT'C
Cuts at: 0 1976 4434
Size: 1976 2458
AccI GT'mk_AC
Cuts at: 0 3660 4318 4434
Size: 3660 658 116
Fragments arranged by size:
3660 658 116
AclI AA'CG_TT
Cuts at: 0 2940 4434
Size: 2940 1494
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 433 516 578 1088 1748 2900 3521
Size: 209 224 83 62 510 660 1152 621
Cuts at: 3521 4069 4434
Size: 548 365
Fragments arranged by size:
1152 660 621 548 510 365 224 209
83 62
AflII C'TTAA_G
Cuts at: 0 103 4434
Size: 103 4331
AflIII A'CryG_T
Cuts at: 0 2048 4434
Size: 2048 2386
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1483 2042 4434
Size: 1483 559 2392
Fragments arranged by size:
2392 1483 559
AlwNI CAG_nnn'CTG
Cuts at: 0 2035 3307 4434
Size: 2035 1272 1127
Fragments arranged by size:
2035 1272 1127
ApaLI G'TGCA_C
Cuts at: 0 3068 3245 4434
Size: 3068 177 1189
Fragments arranged by size:
3068 1189 177
ApoI r'AATT_y
Cuts at: 0 224 292 472 790 1254 1901 3799 4405
Size: 224 68 180 318 464 647 1898 606
Cuts at: 4405 4434
Size: 29
Fragments arranged by size:
1898 647 606 464 318 224 180 68
29
AseI AT'TA_AT
Cuts at: 0 998 3509 4434
Size: 998 2511 925
Fragments arranged by size:
2511 998 925
AvaI C'yCGr_G
Cuts at: 0 2850 4169 4434
Size: 2850 1319 265
Fragments arranged by size:
2850 1319 265
AvrII C'CTAG_G
Cuts at: 0 499 4434
Size: 499 3935
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2240 2273 4434
Size: 2240 33 2161
Fragments arranged by size:
2240 2161 33
BanI G'GyrC_C
Cuts at: 0 2428 4434
Size: 2428 2006
BanII G_rGCy'C
Cuts at: 0 718 2707 3633 4083 4434
Size: 718 1989 926 450 351
Fragments arranged by size:
1989 926 718 450 351
BbsI GAAGACnn'nnnn_
Cuts at: 0 1172 2354 2486 3467 4434
Size: 1172 1182 132 981 967
Fragments arranged by size:
1182 1172 981 967 132
BbvCI CC'TCA_GC
Cuts at: 0 1388 3086 3611 4434
Size: 1388 1698 525 823
Fragments arranged by size:
1698 1388 823 525
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3826 3860 4434
Size: 3826 34 574
Fragments arranged by size:
3826 574 34
BglII A'GATC_T
Cuts at: 0 2356 4434
Size: 2356 2078
Bme1580I G_kGCm'C
Cuts at: 0 2433 3072 3249 4434
Size: 2433 639 177 1185
Fragments arranged by size:
2433 1185 639 177
BmrI ACTGGGnnnn_n'
Cuts at: 0 2239 3128 3403 4434
Size: 2239 889 275 1031
Fragments arranged by size:
2239 1031 889 275
BmtI G_CTAG'C
Cuts at: 0 4352 4434
Size: 4352 82
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 693 695 732 2385 3395 4434
Size: 693 2 37 1653 1010 1039
Fragments arranged by size:
1653 1039 1010 693 37 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 368 1298 1382 1394 1562 1983 2233 2624
Size: 368 930 84 12 168 421 250 391
Cuts at: 2624 2774 4271 4382 4434
Size: 150 1497 111 52
Fragments arranged by size:
1497 930 421 391 368 250 168 150
111 84 52 12
Bpu10I CC'TnA_GC
Cuts at: 0 1388 1888 3077 3086 3611 3848 4222 4434
Size: 1388 500 1189 9 525 237 374 212
Fragments arranged by size:
1388 1189 525 500 374 237 212 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 318 1490 2850 3793 4434
Size: 22 296 1172 1360 943 641
Fragments arranged by size:
1360 1172 943 641 296 22
BsaI GGTCTCn'nnnn_
Cuts at: 0 1159 1951 2724 4434
Size: 1159 792 773 1710
Fragments arranged by size:
1710 1159 792 773
BsaBI GATnn'nnATC
Cuts at: 0 635 2009 3524 4434
Size: 635 1374 1515 910
Fragments arranged by size:
1515 1374 910 635
BsaHI Gr'CG_yC
Cuts at: 0 1973 2760 4434
Size: 1973 787 1674
Fragments arranged by size:
1973 1674 787
BsaWI w'CCGG_w
Cuts at: 0 2726 3968 4434
Size: 2726 1242 466
Fragments arranged by size:
2726 1242 466
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1769 1799 2433 2463 2925 2955 4434
Size: 1769 30 634 30 462 30 1479
Fragments arranged by size:
1769 1479 634 462 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 324 528 2209 2438 2853 2885 4381 4434
Size: 324 204 1681 229 415 32 1496 53
Fragments arranged by size:
1681 1496 415 324 229 204 53 32
BseYI C'CCAG_C
Cuts at: 0 1568 2278 2746 3052 4434
Size: 1568 710 468 306 1382
Fragments arranged by size:
1568 1382 710 468 306
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1178 2088 2385 2810 4112 4201 4345 4434
Size: 1178 910 297 425 1302 89 144 89
Fragments arranged by size:
1302 1178 910 425 297 144 89 89
BsiHKAI G_wGCw'C
Cuts at: 0 2707 3072 3249 4083 4204 4434
Size: 2707 365 177 834 121 230
Fragments arranged by size:
2707 834 365 230 177 121
BsmI GAATG_Cn'
Cuts at: 0 2656 2670 3117 4434
Size: 2656 14 447 1317
Fragments arranged by size:
2656 1317 447 14
Bsp1286I G_dGCh'C
Cuts at: 0 718 2433 2707 3072 3249 3633 4083 4204
Size: 718 1715 274 365 177 384 450 121
Cuts at: 4204 4434
Size: 230
Fragments arranged by size:
1715 718 450 384 365 274 230 177
121
BspEI T'CCGG_A
Cuts at: 0 2726 4434
Size: 2726 1708
BspHI T'CATG_A
Cuts at: 0 3177 4032 4434
Size: 3177 855 402
Fragments arranged by size:
3177 855 402
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1017 2020 4434
Size: 1017 1003 2414
Fragments arranged by size:
2414 1017 1003
BsrBI CCG'CTC
Cuts at: 0 4255 4434
Size: 4255 179
BsrDI GCAATG_nn'
Cuts at: 0 917 1061 3152 3890 4434
Size: 917 144 2091 738 544
Fragments arranged by size:
2091 917 738 544 144
BsrFI r'CCGG_y
Cuts at: 0 2756 4434
Size: 2756 1678
BsrGI T'GTAC_A
Cuts at: 0 860 4434
Size: 860 3574
BstAPI GCAn_nnn'nTGC
Cuts at: 0 139 2035 4434
Size: 139 1896 2399
Fragments arranged by size:
2399 1896 139
BstBI TT'CG_AA
Cuts at: 0 2694 4434
Size: 2694 1740
BstXI CCAn_nnnn'nTGG
Cuts at: 0 228 4434
Size: 228 4206
BstYI r'GATC_y
Cuts at: 0 651 1077 2356 2565 4434
Size: 651 426 1279 209 1869
Fragments arranged by size:
1869 1279 651 426 209
Bsu36I CC'TnA_GG
Cuts at: 0 1554 4434
Size: 1554 2880
BtgI C'CryG_G
Cuts at: 0 1665 4117 4434
Size: 1665 2452 317
Fragments arranged by size:
2452 1665 317
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2534 2912 4434
Size: 2534 378 1522
Fragments arranged by size:
2534 1522 378
BtsI GCAGTG_nn'
Cuts at: 0 1166 1710 3271 4189 4434
Size: 1166 544 1561 918 245
Fragments arranged by size:
1561 1166 918 544 245
ClaI AT'CG_AT
Cuts at: 0 1428 3738 4421 4434
Size: 1428 2310 683 13
Fragments arranged by size:
2310 1428 683 13
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 755 790 4292 4327 4434
Size: 755 35 3502 35 107
Fragments arranged by size:
3502 755 107 35 35
DraI TTT'AAA
Cuts at: 0 546 4434
Size: 546 3888
DraIII CAC_nnn'GTG
Cuts at: 0 379 4434
Size: 379 4055
EaeI y'GGCC_r
Cuts at: 0 1337 2474 3731 3944 4120 4229 4434
Size: 1337 1137 1257 213 176 109 205
Fragments arranged by size:
1337 1257 1137 213 205 176 109
EarI CTCTTCn'nnn_
Cuts at: 0 1064 1258 1529 2768 4088 4434
Size: 1064 194 271 1239 1320 346
Fragments arranged by size:
1320 1239 1064 346 271 194
EcoICRI GAG'CTC
Cuts at: 0 2705 4081 4434
Size: 2705 1376 353
Fragments arranged by size:
2705 1376 353
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 368 433 516 578 1088 1298 1382
Size: 209 159 65 83 62 510 210 84
Cuts at: 1382 1394 1562 1748 1983 2233 2624 2774 2900
Size: 12 168 186 235 250 391 150 126
Cuts at: 2900 3521 4069 4271 4382 4434
Size: 621 548 202 111 52
Fragments arranged by size:
621 548 510 391 250 235 210 209
202 186 168 159 150 126 111 84
83 65 62 52 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 40 448 2188 4434
Size: 40 408 1740 2246
Fragments arranged by size:
2246 1740 408 40
EcoO109I rG'GnC_Cy
Cuts at: 0 1564 1602 1732 1804 2175 3019 4434
Size: 1564 38 130 72 371 844 1415
Fragments arranged by size:
1564 1415 844 371 130 72 38
EcoRI G'AATT_C
Cuts at: 0 292 3799 4405 4434
Size: 292 3507 606 29
Fragments arranged by size:
3507 606 292 29
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2156 2690 3943 4002 4434
Size: 2156 534 1253 59 432
Fragments arranged by size:
2156 1253 534 432 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 300 332 837 869 1771 1803 2341 2373
Size: 300 32 505 32 902 32 538 32
Cuts at: 2373 2433 2465 2476 2508 2701 2733 2893 2925
Size: 60 32 11 32 193 32 160 32
Cuts at: 2925 3016 3048 4056 4088 4434
Size: 91 32 1008 32 346
Fragments arranged by size:
1008 902 538 505 346 300 193 160
91 60 32 32 32 32 32 32
32 32 32 32 11
HincII GTy'rAC
Cuts at: 0 3661 4434
Size: 3661 773
HindIII A'AGCT_T
Cuts at: 0 191 2138 4434
Size: 191 1947 2296
Fragments arranged by size:
2296 1947 191
MfeI C'AATT_G
Cuts at: 0 262 414 4434
Size: 262 152 4020
Fragments arranged by size:
4020 262 152
MscI TGG'CCA
Cuts at: 0 2476 3733 3946 4122 4231 4434
Size: 2476 1257 213 176 109 203
Fragments arranged by size:
2476 1257 213 203 176 109
MslI CAynn'nnrTG
Cuts at: 0 1465 4434
Size: 1465 2969
MspA1I CmG'CkG
Cuts at: 0 112 1839 2439 2644 2750 2794 3832 3942
Size: 112 1727 600 205 106 44 1038 110
Cuts at: 3942 4434
Size: 492
Fragments arranged by size:
1727 1038 600 492 205 112 110 106
44
NcoI C'CATG_G
Cuts at: 0 1665 4117 4434
Size: 1665 2452 317
Fragments arranged by size:
2452 1665 317
NdeI CA'TA_TG
Cuts at: 0 1484 3901 4434
Size: 1484 2417 533
Fragments arranged by size:
2417 1484 533
NheI G'CTAG_C
Cuts at: 0 4348 4434
Size: 4348 86
NruI TCG'CGA
Cuts at: 0 4019 4434
Size: 4019 415
NspI r_CATG'y
Cuts at: 0 220 1614 2052 4434
Size: 220 1394 438 2382
Fragments arranged by size:
2382 1394 438 220
PciI A'CATG_T
Cuts at: 0 2048 4434
Size: 2048 2386
PflMI CCAn_nnn'nTGG
Cuts at: 0 2620 4434
Size: 2620 1814
PfoI T'CCnGG_A
Cuts at: 0 1517 2576 2763 4434
Size: 1517 1059 187 1671
Fragments arranged by size:
1671 1517 1059 187
PpuMI rG'GwC_Cy
Cuts at: 0 1564 1602 1732 1804 4434
Size: 1564 38 130 72 2630
Fragments arranged by size:
2630 1564 130 72 38
PsiI TTA'TAA
Cuts at: 0 4160 4434
Size: 4160 274
PstI C_TGCA'G
Cuts at: 0 954 1705 2040 2799 3278 3869 4434
Size: 954 751 335 759 479 591 565
Fragments arranged by size:
954 759 751 591 565 479 335
PvuII CAG'CTG
Cuts at: 0 112 1839 2439 2644 2750 2794 3832 3942
Size: 112 1727 600 205 106 44 1038 110
Cuts at: 3942 4434
Size: 492
Fragments arranged by size:
1727 1038 600 492 205 112 110 106
44
SacI G_AGCT'C
Cuts at: 0 2707 4083 4434
Size: 2707 1376 351
Fragments arranged by size:
2707 1376 351
SalI G'TCGA_C
Cuts at: 0 3659 4434
Size: 3659 775
SapI GCTCTTCn'nnn_
Cuts at: 0 2768 4088 4434
Size: 2768 1320 346
Fragments arranged by size:
2768 1320 346
SexAI A'CCwGG_T
Cuts at: 0 4022 4434
Size: 4022 412
SfcI C'TryA_G
Cuts at: 0 950 1230 1436 1701 1834 2036 2795 3274
Size: 950 280 206 265 133 202 759 479
Cuts at: 3274 3618 3670 3865 4434
Size: 344 52 195 569
Fragments arranged by size:
950 759 569 479 344 280 265 206
202 195 133 52
SmaI CCC'GGG
Cuts at: 0 4171 4434
Size: 4171 263
SmlI C'TyrA_G
Cuts at: 0 37 103 297 1505 2865 3772 4434
Size: 37 66 194 1208 1360 907 662
Fragments arranged by size:
1360 1208 907 662 194 66 37
SpeI A'CTAG_T
Cuts at: 0 2398 2688 4434
Size: 2398 290 1746
Fragments arranged by size:
2398 1746 290
SspI AAT'ATT
Cuts at: 0 2001 4434
Size: 2001 2433
StuI AGG'CCT
Cuts at: 0 2291 4434
Size: 2291 2143
StyI C'CwwG_G
Cuts at: 0 499 1098 1665 2926 4117 4434
Size: 499 599 567 1261 1191 317
Fragments arranged by size:
1261 1191 599 567 499 317
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3220 4434
Size: 3220 1214
TatI w'GTAC_w
Cuts at: 0 99 860 916 1966 3163 4434
Size: 99 761 56 1050 1197 1271
Fragments arranged by size:
1271 1197 1050 761 99 56
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1769 1801 3033 3065 4137 4169 4434
Size: 1769 32 1232 32 1072 32 265
Fragments arranged by size:
1769 1232 1072 265 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 4243 4434
Size: 4243 191
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1470 4434
Size: 1470 2964
XmaI C'CCGG_G
Cuts at: 0 4169 4434
Size: 4169 265
XmnI GAAnn'nnTTC
Cuts at: 0 981 3803 3860 3926 4434
Size: 981 2822 57 66 508
Fragments arranged by size:
2822 981 508 66 57
ZraI GAC'GTC
Cuts at: 0 1974 4434
Size: 1974 2460
Enzymes that do cut:
AarI AatII AccI AclI AcuI AflII AflIII AlfI
AlwNI ApaLI ApoI AseI AvaI AvrII BaeI BanI
BanII BbsI BbvCI BcgI BglII Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI Hin4I HincII HindIII MfeI MscI MslI
MspA1I NcoI NdeI NheI NruI NspI PciI PflMI
PfoI PpuMI PsiI PstI PvuII SacI SalI SapI
SexAI SfcI SmaI SmlI SpeI SspI StuI StyI
TaqII TatI TstI Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AfeI AgeI AhdI AleI AloI ApaI AscI
AsiSI BamHI BbeI BciVI BclI BfrBI BglI BlpI
BmgBI BsaAI BsiEI BsiWI BsmBI BssHII BssSI BstEII
BstZ17I DrdI EagI EciI EcoRV FseI FspI FspAI
HaeII HpaI KasI KpnI MluI MmeI NaeI NarI
NgoMIV NotI NsiI PacI PasI PmeI PmlI PpiI
PshAI PspOMI PspXI PsrI PvuI RsrII SacII SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SphI
SrfI SwaI XbaI XhoI