Restriction map for
pF1KA1522
(Linear) (Six-base) MAPSORT of: KIAA1522.seq Check: 4460 from: 1 to: 3138
KIAA1522.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AccI GT'mk_AC
Cuts at: 0 752 3138
Size: 752 2386
Acc65I G'GTAC_C
Cuts at: 0 787 1209 1530 3138
Size: 787 422 321 1608
Fragments arranged by size:
1608 787 422 321
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1357 2255 3138
Size: 1357 898 883
Fragments arranged by size:
1357 898 883
AgeI A'CCGG_T
Cuts at: 0 2433 3138
Size: 2433 705
AleI CACnn'nnGTG
Cuts at: 0 1129 3138
Size: 1129 2009
AlwNI CAG_nnn'CTG
Cuts at: 0 56 94 151 273 687 832 856 1399
Size: 56 38 57 122 414 145 24 543
Cuts at: 1399 2131 2199 2323 2386 2626 3138
Size: 732 68 124 63 240 512
Fragments arranged by size:
732 543 512 414 240 145 124 122
68 63 57 56 38 24
ApaI G_GGCC'C
Cuts at: 0 669 798 2601 3088 3138
Size: 669 129 1803 487 50
Fragments arranged by size:
1803 669 487 129 50
ApaLI G'TGCA_C
Cuts at: 0 602 3138
Size: 602 2536
AvaI C'yCGr_G
Cuts at: 0 41 590 644 668 791 1608 2343 2981
Size: 41 549 54 24 123 817 735 638
Cuts at: 2981 3108 3138
Size: 127 30
Fragments arranged by size:
817 735 638 549 127 123 54 41
30 24
AvrII C'CTAG_G
Cuts at: 0 960 3138
Size: 960 2178
BanI G'GyrC_C
Cuts at: 0 576 649 787 1209 1530 1559 1851 2112
Size: 576 73 138 422 321 29 292 261
Cuts at: 2112 3138
Size: 1026
Fragments arranged by size:
1026 576 422 321 292 261 138 73
29
BanII G_rGCy'C
Cuts at: 0 242 391 597 669 798 866 874 1189
Size: 242 149 206 72 129 68 8 315
Cuts at: 1189 1243 1894 2144 2550 2601 2645 2788 2993
Size: 54 651 250 406 51 44 143 205
Cuts at: 2993 3020 3088 3115 3138
Size: 27 68 27 23
Fragments arranged by size:
651 406 315 250 242 206 205 149
143 129 72 68 68 54 51 44
27 27 23 8
BbeI G_GCGC'C
Cuts at: 0 1563 3138
Size: 1563 1575
BbsI GAAGACnn'nnnn_
Cuts at: 0 171 347 3138
Size: 171 176 2791
Fragments arranged by size:
2791 176 171
BbvCI CC'TCA_GC
Cuts at: 0 1265 2135 2316 3138
Size: 1265 870 181 822
Fragments arranged by size:
1265 870 822 181
BciVI GTATCCnnnnn_n'
Cuts at: 0 322 1597 3138
Size: 322 1275 1541
Fragments arranged by size:
1541 1275 322
BglI GCCn_nnn'nGGC
Cuts at: 0 647 1404 2273 2761 3110 3138
Size: 647 757 869 488 349 28
Fragments arranged by size:
869 757 647 488 349 28
BlpI GC'TnA_GC
Cuts at: 0 1389 2705 2987 3138
Size: 1389 1316 282 151
Fragments arranged by size:
1389 1316 282 151
Bme1580I G_kGCm'C
Cuts at: 0 606 669 798 2601 3088 3138
Size: 606 63 129 1803 487 50
Fragments arranged by size:
1803 606 487 129 63 50
BmgBI CAC'GTC
Cuts at: 0 951 1706 1748 3138
Size: 951 755 42 1390
Fragments arranged by size:
1390 951 755 42
BmrI ACTGGGnnnn_n'
Cuts at: 0 182 1241 2572 2614 2739 2966 3138
Size: 182 1059 1331 42 125 227 172
Fragments arranged by size:
1331 1059 227 182 172 125 42
BmtI G_CTAG'C
Cuts at: 0 1199 3138
Size: 1199 1939
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1127 1259 3138
Size: 1127 132 1879
Fragments arranged by size:
1879 1127 132
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 709 1038 1074 1122 1581 2348 2522 2824
Size: 709 329 36 48 459 767 174 302
Cuts at: 2824 3138
Size: 314
Fragments arranged by size:
767 709 459 329 314 302 174 48
36
Bpu10I CC'TnA_GC
Cuts at: 0 868 1265 1424 1430 2135 2255 2316 3138
Size: 868 397 159 6 705 120 61 822
Fragments arranged by size:
868 822 705 397 159 120 61 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2647 2682 3138
Size: 2647 35 456
Fragments arranged by size:
2647 456 35
BsaI GGTCTCn'nnnn_
Cuts at: 0 2818 3138
Size: 2818 320
BsaAI yAC'GTr
Cuts at: 0 378 3138
Size: 378 2760
BsaHI Gr'CG_yC
Cuts at: 0 495 1560 1958 3138
Size: 495 1065 398 1180
Fragments arranged by size:
1180 1065 495 398
BsaWI w'CCGG_w
Cuts at: 0 2433 2864 3138
Size: 2433 431 274
Fragments arranged by size:
2433 431 274
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 334 364 394 424 1069 1099 1620 1650
Size: 334 30 30 30 645 30 521 30
Cuts at: 1650 1983 2013 2988 3018 3138
Size: 333 30 975 30 120
Fragments arranged by size:
975 645 521 334 333 120 30 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1035 1077 1143 1725 1752 1755 1829 2152
Size: 1035 42 66 582 27 3 74 323
Cuts at: 2152 2203 2242 2304 2351 2625 2993 3106 3138
Size: 51 39 62 47 274 368 113 32
Fragments arranged by size:
1035 582 368 323 274 113 74 66
62 51 47 42 39 32 27 3
BseYI C'CCAG_C
Cuts at: 0 31 287 522 1014 1032 1448 1515 1526
Size: 31 256 235 492 18 416 67 11
Cuts at: 1526 1679 1773 1899 2379 2556 3138
Size: 153 94 126 480 177 582
Fragments arranged by size:
582 492 480 416 256 235 177 153
126 94 67 31 18 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 562 985 3138
Size: 202 360 423 2153
Fragments arranged by size:
2153 423 360 202
BsiEI CG_ry'CG
Cuts at: 0 73 85 639 3138
Size: 73 12 554 2499
Fragments arranged by size:
2499 554 73 12
BsiHKAI G_wGCw'C
Cuts at: 0 167 391 517 606 1189 2538 2706 3138
Size: 167 224 126 89 583 1349 168 432
Fragments arranged by size:
1349 583 432 224 168 167 126 89
BsiWI C'GTAC_G
Cuts at: 0 615 3138
Size: 615 2523
BsmI GAATG_Cn'
Cuts at: 0 1286 3138
Size: 1286 1852
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1056 1712 1737 3138
Size: 1056 656 25 1401
Fragments arranged by size:
1401 1056 656 25
Bsp1286I G_dGCh'C
Cuts at: 0 167 242 391 517 597 606 669 798
Size: 167 75 149 126 80 9 63 129
Cuts at: 798 866 874 1189 1243 1894 2144 2538 2550
Size: 68 8 315 54 651 250 394 12
Cuts at: 2550 2601 2645 2706 2788 2993 3020 3088 3115
Size: 51 44 61 82 205 27 68 27
Cuts at: 3115 3138
Size: 23
Fragments arranged by size:
651 394 315 250 205 167 149 129
126 82 80 75 68 68 63 61
54 51 44 27 27 23 12 9
8
BspEI T'CCGG_A
Cuts at: 0 2864 3138
Size: 2864 274
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1642 3138
Size: 1642 1496
BsrBI CCG'CTC
Cuts at: 0 245 419 507 1028 1790 2808 3138
Size: 245 174 88 521 762 1018 330
Fragments arranged by size:
1018 762 521 330 245 174 88
BsrFI r'CCGG_y
Cuts at: 0 84 776 2433 3138
Size: 84 692 1657 705
Fragments arranged by size:
1657 705 692 84
BstAPI GCAn_nnn'nTGC
Cuts at: 0 94 540 610 856 2119 2514 3138
Size: 94 446 70 246 1263 395 624
Fragments arranged by size:
1263 624 446 395 246 94 70
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1647 3138
Size: 1647 1491
BstYI r'GATC_y
Cuts at: 0 2151 3138
Size: 2151 987
Bsu36I CC'TnA_GG
Cuts at: 0 559 654 2186 2637 2723 2912 3138
Size: 559 95 1532 451 86 189 226
Fragments arranged by size:
1532 559 451 226 189 95 86
BtgI C'CryG_G
Cuts at: 0 294 782 1567 2204 2528 2734 3138
Size: 294 488 785 637 324 206 404
Fragments arranged by size:
785 637 488 404 324 294 206
BtsI GCAGTG_nn'
Cuts at: 0 161 833 1236 1259 1302 1400 2002 2587
Size: 161 672 403 23 43 98 602 585
Cuts at: 2587 3138
Size: 551
Fragments arranged by size:
672 602 585 551 403 161 98 43
23
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1787 1822 2436 2471 3138
Size: 1787 35 614 35 667
Fragments arranged by size:
1787 667 614 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1828 3138
Size: 1828 1310
EaeI y'GGCC_r
Cuts at: 0 82 135 636 774 875 1007 1311 1459
Size: 82 53 501 138 101 132 304 148
Cuts at: 1459 2246 2411 2678 2762 2884 3138
Size: 787 165 267 84 122 254
Fragments arranged by size:
787 501 304 267 254 165 148 138
132 122 101 84 82 53
EagI C'GGCC_G
Cuts at: 0 82 636 3138
Size: 82 554 2502
Fragments arranged by size:
2502 554 82
EarI CTCTTCn'nnn_
Cuts at: 0 206 1715 1743 3138
Size: 206 1509 28 1395
Fragments arranged by size:
1509 1395 206 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 525 709 715 2360 2385 3070 3138
Size: 525 184 6 1645 25 685 68
Fragments arranged by size:
1645 685 525 184 68 25 6
EcoICRI GAG'CTC
Cuts at: 0 389 1187 3138
Size: 389 798 1951
Fragments arranged by size:
1951 798 389
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 709 1038 1074 1122 1357 1581 2255 2348
Size: 709 329 36 48 235 224 674 93
Cuts at: 2348 2522 2824 3138
Size: 174 302 314
Fragments arranged by size:
709 674 329 314 302 235 224 174
93 48 36
EcoO109I rG'GnC_Cy
Cuts at: 0 27 130 665 795 853 1644 1654 1724
Size: 27 103 535 130 58 791 10 70
Cuts at: 1724 2196 2266 2597 2598 2895 3138
Size: 472 70 331 1 297 243
Fragments arranged by size:
791 535 472 331 297 243 130 103
70 70 58 27 10 1
FspI TGC'GCA
Cuts at: 0 979 3138
Size: 979 2159
HaeII r_GCGC'y
Cuts at: 0 688 713 738 1041 1563 3138
Size: 688 25 25 303 522 1575
Fragments arranged by size:
1575 688 522 303 25 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 334 366 394 426 2101 2133 3138
Size: 334 32 28 32 1675 32 1005
Fragments arranged by size:
1675 1005 334 32 32 32 28
KasI G'GCGC_C
Cuts at: 0 1559 3138
Size: 1559 1579
KpnI G_GTAC'C
Cuts at: 0 791 1213 1534 3138
Size: 791 422 321 1604
Fragments arranged by size:
1604 791 422 321
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 477 1145 2682 3138
Size: 477 668 1537 456
Fragments arranged by size:
1537 668 477 456
MscI TGG'CCA
Cuts at: 0 137 2248 2413 3138
Size: 137 2111 165 725
Fragments arranged by size:
2111 725 165 137
MslI CAynn'nnrTG
Cuts at: 0 122 1129 3138
Size: 122 1007 2009
Fragments arranged by size:
2009 1007 122
MspA1I CmG'CkG
Cuts at: 0 35 968 992 1383 1452 1526 1903 2802
Size: 35 933 24 391 69 74 377 899
Cuts at: 2802 2844 2883 3078 3138
Size: 42 39 195 60
Fragments arranged by size:
933 899 391 377 195 74 69 60
42 39 35 24
NaeI GCC'GGC
Cuts at: 0 86 3138
Size: 86 3052
NarI GG'CG_CC
Cuts at: 0 1560 3138
Size: 1560 1578
NcoI C'CATG_G
Cuts at: 0 294 2204 3138
Size: 294 1910 934
Fragments arranged by size:
1910 934 294
NgoMIV G'CCGG_C
Cuts at: 0 84 3138
Size: 84 3054
NheI G'CTAG_C
Cuts at: 0 1195 3138
Size: 1195 1943
NspI r_CATG'y
Cuts at: 0 541 928 2410 3138
Size: 541 387 1482 728
Fragments arranged by size:
1482 728 541 387
PasI CC'CwG_GG
Cuts at: 0 1221 1720 1815 2401 2465 3138
Size: 1221 499 95 586 64 673
Fragments arranged by size:
1221 673 586 499 95 64
PflMI CCAn_nnn'nTGG
Cuts at: 0 1526 3138
Size: 1526 1612
PfoI T'CCnGG_A
Cuts at: 0 322 2538 3138
Size: 322 2216 600
Fragments arranged by size:
2216 600 322
PpuMI rG'GwC_Cy
Cuts at: 0 1654 2196 3138
Size: 1654 542 942
Fragments arranged by size:
1654 942 542
PshAI GACnn'nnGTC
Cuts at: 0 1961 3138
Size: 1961 1177
PspOMI G'GGCC_C
Cuts at: 0 665 794 2597 3084 3138
Size: 665 129 1803 487 54
Fragments arranged by size:
1803 665 487 129 54
PspXI vC'TCGA_Gb
Cuts at: 0 1608 2981 3138
Size: 1608 1373 157
Fragments arranged by size:
1608 1373 157
PstI C_TGCA'G
Cuts at: 0 156 361 683 734 861 973 997 2009
Size: 156 205 322 51 127 112 24 1012
Cuts at: 2009 2265 2510 2674 2801 3077 3138
Size: 256 245 164 127 276 61
Fragments arranged by size:
1012 322 276 256 245 205 164 156
127 127 112 61 51 24
PvuII CAG'CTG
Cuts at: 0 1452 1526 1903 2802 3078 3138
Size: 1452 74 377 899 276 60
Fragments arranged by size:
1452 899 377 276 74 60
RsrII CG'GwC_CG
Cuts at: 0 2655 2716 3138
Size: 2655 61 422
Fragments arranged by size:
2655 422 61
SacI G_AGCT'C
Cuts at: 0 391 1189 3138
Size: 391 798 1949
Fragments arranged by size:
1949 798 391
SapI GCTCTTCn'nnn_
Cuts at: 0 206 3138
Size: 206 2932
SbfI CC_TGCA'GG
Cuts at: 0 683 2265 3138
Size: 683 1582 873
Fragments arranged by size:
1582 873 683
SfcI C'TryA_G
Cuts at: 0 152 357 679 730 857 941 969 993
Size: 152 205 322 51 127 84 28 24
Cuts at: 993 2005 2261 2442 2506 2670 2797 3073 3138
Size: 1012 256 181 64 164 127 276 65
Fragments arranged by size:
1012 322 276 256 205 181 164 152
127 127 84 65 64 51 28 24
SfoI GGC'GCC
Cuts at: 0 1561 3138
Size: 1561 1577
SmaI CCC'GGG
Cuts at: 0 43 592 670 793 2345 3110 3138
Size: 43 549 78 123 1552 765 28
Fragments arranged by size:
1552 765 549 123 78 43 28
SmlI C'TyrA_G
Cuts at: 0 1608 2662 2697 2981 3138
Size: 1608 1054 35 284 157
Fragments arranged by size:
1608 1054 284 157 35
StuI AGG'CCT
Cuts at: 0 1270 2668 2693 3138
Size: 1270 1398 25 445
Fragments arranged by size:
1398 1270 445 25
StyI C'CwwG_G
Cuts at: 0 98 294 960 1640 1847 2204 2780 3138
Size: 98 196 666 680 207 357 576 358
Fragments arranged by size:
680 666 576 358 357 207 196 98
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 319 1094 1479 1524 1807 2097 2521 3043
Size: 319 775 385 45 283 290 424 522
Cuts at: 3043 3138
Size: 95
Fragments arranged by size:
775 522 424 385 319 290 283 95
45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 555 587 1069 1099 1101 1131 1620 1652
Size: 555 32 482 30 2 30 489 32
Cuts at: 1652 3138
Size: 1486
Fragments arranged by size:
1486 555 489 482 32 32 30 30
2
Tth111I GACn'n_nGTC
Cuts at: 0 175 748 3138
Size: 175 573 2390
Fragments arranged by size:
2390 573 175
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 375 1228 1523 2559 3138
Size: 375 853 295 1036 579
Fragments arranged by size:
1036 853 579 375 295
XhoI C'TCGA_G
Cuts at: 0 1608 2981 3138
Size: 1608 1373 157
Fragments arranged by size:
1608 1373 157
XmaI C'CCGG_G
Cuts at: 0 41 590 668 791 2343 3108 3138
Size: 41 549 78 123 1552 765 30
Fragments arranged by size:
1552 765 549 123 78 41 30
Enzymes that do cut:
AccI Acc65I AcuI AgeI AleI AlwNI ApaI ApaLI
AvaI AvrII BanI BanII BbeI BbsI BbvCI BciVI
BglI BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrFI BstAPI BstXI BstYI Bsu36I
BtgI BtsI CspCI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I FspI HaeII Hin4I KasI KpnI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PasI PflMI PfoI PpuMI PshAI PspOMI
PspXI PstI PvuII RsrII SacI SapI SbfI SfcI
SfoI SmaI SmlI StuI StyI TaqII TstI Tth111I
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AhdI AlfI
AloI ApoI AscI AseI AsiSI BaeI BamHI BcgI
BclI BfrBI BglII BsaBI BspHI BsrDI BsrGI BssHII
BssSI BstBI BstEII BstZ17I BtgZI ClaI DraI DraIII
EcoNI EcoRI EcoRV FalI FseI FspAI HincII HindIII
HpaI MfeI MluI NdeI NotI NruI NsiI PacI
PciI PmeI PmlI PpiI PsiI PsrI PvuI SacII
SalI SanDI ScaI SexAI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI TatI XbaI XmnI
ZraI