Restriction map for
pF1KA1533
(Linear) (Six-base) MAPSORT of: KIAA1533.seq Check: 9043 from: 1 to: 2175
KIAA1533.orf
With 182 enzymes: SgfI *
November 13, 2006 11:21 ..
AccI GT'mk_AC
Cuts at: 0 911 2175
Size: 911 1264
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 496 2175
Size: 478 18 1679
Fragments arranged by size:
1679 478 18
AflIII A'CryG_T
Cuts at: 0 469 2175
Size: 469 1706
AhdI GACnn_n'nnGTC
Cuts at: 0 626 2175
Size: 626 1549
AleI CACnn'nnGTG
Cuts at: 0 133 1228 2175
Size: 133 1095 947
Fragments arranged by size:
1095 947 133
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 253 2175
Size: 253 1922
AlwNI CAG_nnn'CTG
Cuts at: 0 90 1350 1732 2055 2175
Size: 90 1260 382 323 120
Fragments arranged by size:
1260 382 323 120 90
ApaI G_GGCC'C
Cuts at: 0 564 998 1012 1318 1718 1813 2175
Size: 564 434 14 306 400 95 362
Fragments arranged by size:
564 434 400 362 306 95 14
ApaLI G'TGCA_C
Cuts at: 0 2035 2175
Size: 2035 140
AvaI C'yCGr_G
Cuts at: 0 783 857 966 1649 1745 1764 1813 2175
Size: 783 74 109 683 96 19 49 362
Fragments arranged by size:
783 683 362 109 96 74 49 19
AvrII C'CTAG_G
Cuts at: 0 201 2175
Size: 201 1974
BanI G'GyrC_C
Cuts at: 0 127 174 644 2175
Size: 127 47 470 1531
Fragments arranged by size:
1531 470 127 47
BanII G_rGCy'C
Cuts at: 0 265 564 641 980 998 1012 1243 1318
Size: 265 299 77 339 18 14 231 75
Cuts at: 1318 1604 1704 1718 1771 1813 2075 2175
Size: 286 100 14 53 42 262 100
Fragments arranged by size:
339 299 286 265 262 231 100 100
77 75 53 42 18 14 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 1131 1487 1580 2175
Size: 1131 356 93 595
Fragments arranged by size:
1131 595 356 93
BbvCI CC'TCA_GC
Cuts at: 0 1774 2175
Size: 1774 401
BglI GCCn_nnn'nGGC
Cuts at: 0 993 2175
Size: 993 1182
BglII A'GATC_T
Cuts at: 0 518 2175
Size: 518 1657
BlpI GC'TnA_GC
Cuts at: 0 259 1154 1237 2175
Size: 259 895 83 938
Fragments arranged by size:
938 895 259 83
Bme1580I G_kGCm'C
Cuts at: 0 564 998 1012 1318 1718 1746 1813 1836
Size: 564 434 14 306 400 28 67 23
Cuts at: 1836 2039 2175
Size: 203 136
Fragments arranged by size:
564 434 400 306 203 136 67 28
23 14
BmgBI CAC'GTC
Cuts at: 0 1230 2175
Size: 1230 945
BmrI ACTGGGnnnn_n'
Cuts at: 0 811 1022 1082 1321 1823 2175
Size: 811 211 60 239 502 352
Fragments arranged by size:
811 502 352 239 211 60
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1572 2175
Size: 1572 603
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 918 1147 1180 1266 1612 1648 2119
Size: 358 560 229 33 86 346 36 471
Cuts at: 2119 2175
Size: 56
Fragments arranged by size:
560 471 358 346 229 86 56 36
33
Bpu10I CC'TnA_GC
Cuts at: 0 113 766 1690 1774 2175
Size: 113 653 924 84 401
Fragments arranged by size:
924 653 401 113 84
BsaI GGTCTCn'nnnn_
Cuts at: 0 430 2175
Size: 430 1745
BsaBI GATnn'nnATC
Cuts at: 0 448 2175
Size: 448 1727
BsaHI Gr'CG_yC
Cuts at: 0 926 2175
Size: 926 1249
BsaWI w'CCGG_w
Cuts at: 0 71 293 2175
Size: 71 222 1882
Fragments arranged by size:
1882 222 71
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 691 721 763 793 916 946 2175
Size: 691 30 42 30 123 30 1229
Fragments arranged by size:
1229 691 123 42 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 147 571 771 904 916 921 1048 1050
Size: 147 424 200 133 12 5 127 2
Cuts at: 1050 1114 2175
Size: 64 1061
Fragments arranged by size:
1061 424 200 147 133 127 64 12
5 2
BseYI C'CCAG_C
Cuts at: 0 187 431 675 983 1718 2147 2175
Size: 187 244 244 308 735 429 28
Fragments arranged by size:
735 429 308 244 244 187 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 507 2092 2175
Size: 507 1585 83
Fragments arranged by size:
1585 507 83
BsiEI CG_ry'CG
Cuts at: 0 1119 2175
Size: 1119 1056
BsiHKAI G_wGCw'C
Cuts at: 0 25 49 641 973 1604 2039 2075 2175
Size: 25 24 592 332 631 435 36 100
Fragments arranged by size:
631 592 435 332 100 36 25 24
BsmI GAATG_Cn'
Cuts at: 0 609 1754 2175
Size: 609 1145 421
Fragments arranged by size:
1145 609 421
Bsp1286I G_dGCh'C
Cuts at: 0 25 49 265 564 641 973 980 998
Size: 25 24 216 299 77 332 7 18
Cuts at: 998 1012 1243 1318 1604 1704 1718 1746 1771
Size: 14 231 75 286 100 14 28 25
Cuts at: 1771 1813 1836 2039 2075 2175
Size: 42 23 203 36 100
Fragments arranged by size:
332 299 286 231 216 203 100 100
77 75 42 36 28 25 25 24
23 18 14 14 7
BspEI T'CCGG_A
Cuts at: 0 71 293 2175
Size: 71 222 1882
Fragments arranged by size:
1882 222 71
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 897 2175
Size: 897 1278
BsrBI CCG'CTC
Cuts at: 0 1158 1565 2175
Size: 1158 407 610
Fragments arranged by size:
1158 610 407
BsrFI r'CCGG_y
Cuts at: 0 101 1372 1750 2175
Size: 101 1271 378 425
Fragments arranged by size:
1271 425 378 101
BssSI C'ACGA_G
Cuts at: 0 1557 2175
Size: 1557 618
BstAPI GCAn_nnn'nTGC
Cuts at: 0 90 651 2055 2175
Size: 90 561 1404 120
Fragments arranged by size:
1404 561 120 90
BstXI CCAn_nnnn'nTGG
Cuts at: 0 747 1005 1836 2175
Size: 747 258 831 339
Fragments arranged by size:
831 747 339 258
BstYI r'GATC_y
Cuts at: 0 365 404 518 1017 1502 1728 1997 2051
Size: 365 39 114 499 485 226 269 54
Cuts at: 2051 2175
Size: 124
Fragments arranged by size:
499 485 365 269 226 124 114 54
39
BtgI C'CryG_G
Cuts at: 0 131 831 879 1331 1522 2066 2175
Size: 131 700 48 452 191 544 109
Fragments arranged by size:
700 544 452 191 131 109 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 50 1854 2175
Size: 50 1804 321
Fragments arranged by size:
1804 321 50
BtsI GCAGTG_nn'
Cuts at: 0 607 1219 1436 2175
Size: 607 612 217 739
Fragments arranged by size:
739 612 607 217
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 800 835 2175
Size: 800 35 1340
Fragments arranged by size:
1340 800 35
DraIII CAC_nnn'GTG
Cuts at: 0 651 1988 2028 2175
Size: 651 1337 40 147
Fragments arranged by size:
1337 651 147 40
EaeI y'GGCC_r
Cuts at: 0 39 491 834 1116 1955 1979 2175
Size: 39 452 343 282 839 24 196
Fragments arranged by size:
839 452 343 282 196 39 24
EagI C'GGCC_G
Cuts at: 0 1116 2175
Size: 1116 1059
EarI CTCTTCn'nnn_
Cuts at: 0 1068 1180 1894 2175
Size: 1068 112 714 281
Fragments arranged by size:
1068 714 281 112
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 580 1692 2175
Size: 580 1112 483
Fragments arranged by size:
1112 580 483
EcoICRI GAG'CTC
Cuts at: 0 639 1602 2073 2175
Size: 639 963 471 102
Fragments arranged by size:
963 639 471 102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 478 496 918 1147 1180 1266 1612
Size: 358 120 18 422 229 33 86 346
Cuts at: 1612 1648 2119 2175
Size: 36 471 56
Fragments arranged by size:
471 422 358 346 229 120 86 56
36 33 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 373 1957 2175
Size: 373 1584 218
Fragments arranged by size:
1584 373 218
EcoO109I rG'GnC_Cy
Cuts at: 0 182 560 734 1009 1315 1715 1809 1810
Size: 182 378 174 275 306 400 94 1
Cuts at: 1810 2175
Size: 365
Fragments arranged by size:
400 378 365 306 275 182 174 94
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 561 2175
Size: 561 1614
HaeII r_GCGC'y
Cuts at: 0 1154 1275 1328 2008 2175
Size: 1154 121 53 680 167
Fragments arranged by size:
1154 680 167 121 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 130 142 162 174 612 644 691 723
Size: 130 12 20 12 438 32 47 32
Cuts at: 723 743 763 775 795 916 948 1606 1638
Size: 20 20 12 20 121 32 658 32
Cuts at: 1638 2175
Size: 537
Fragments arranged by size:
658 537 438 130 121 47 32 32
32 32 20 20 20 20 12 12
12
MscI TGG'CCA
Cuts at: 0 836 1957 2175
Size: 836 1121 218
Fragments arranged by size:
1121 836 218
MslI CAynn'nnrTG
Cuts at: 0 133 1228 2175
Size: 133 1095 947
Fragments arranged by size:
1095 947 133
MspA1I CmG'CkG
Cuts at: 0 431 456 572 720 793 2175
Size: 431 25 116 148 73 1382
Fragments arranged by size:
1382 431 148 116 73 25
NaeI GCC'GGC
Cuts at: 0 103 1374 1752 2175
Size: 103 1271 378 423
Fragments arranged by size:
1271 423 378 103
NcoI C'CATG_G
Cuts at: 0 131 879 2175
Size: 131 748 1296
Fragments arranged by size:
1296 748 131
NdeI CA'TA_TG
Cuts at: 0 6 2175
Size: 6 2169
NgoMIV G'CCGG_C
Cuts at: 0 101 1372 1750 2175
Size: 101 1271 378 425
Fragments arranged by size:
1271 425 378 101
NspI r_CATG'y
Cuts at: 0 473 2175
Size: 473 1702
PasI CC'CwG_GG
Cuts at: 0 1005 2175
Size: 1005 1170
PciI A'CATG_T
Cuts at: 0 469 2175
Size: 469 1706
PflMI CCAn_nnn'nTGG
Cuts at: 0 1590 1988 2028 2175
Size: 1590 398 40 147
Fragments arranged by size:
1590 398 147 40
PfoI T'CCnGG_A
Cuts at: 0 2075 2175
Size: 2075 100
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2074 2106 2175
Size: 2074 32 69
Fragments arranged by size:
2074 69 32
PpuMI rG'GwC_Cy
Cuts at: 0 182 734 2175
Size: 182 552 1441
Fragments arranged by size:
1441 552 182
PsiI TTA'TAA
Cuts at: 0 272 2175
Size: 272 1903
PspOMI G'GGCC_C
Cuts at: 0 560 994 1008 1314 1714 1809 2175
Size: 560 434 14 306 400 95 366
Fragments arranged by size:
560 434 400 366 306 95 14
PstI C_TGCA'G
Cuts at: 0 86 359 2012 2175
Size: 86 273 1653 163
Fragments arranged by size:
1653 273 163 86
PvuII CAG'CTG
Cuts at: 0 456 720 793 2175
Size: 456 264 73 1382
Fragments arranged by size:
1382 456 264 73
SacI G_AGCT'C
Cuts at: 0 641 1604 2075 2175
Size: 641 963 471 100
Fragments arranged by size:
963 641 471 100
SanDI GG'GwC_CC
Cuts at: 0 182 734 2175
Size: 182 552 1441
Fragments arranged by size:
1441 552 182
SapI GCTCTTCn'nnn_
Cuts at: 0 1180 1894 2175
Size: 1180 714 281
Fragments arranged by size:
1180 714 281
SexAI A'CCwGG_T
Cuts at: 0 647 2175
Size: 647 1528
SfcI C'TryA_G
Cuts at: 0 82 355 414 1207 2008 2175
Size: 82 273 59 793 801 167
Fragments arranged by size:
801 793 273 167 82 59
SmaI CCC'GGG
Cuts at: 0 1651 1747 1815 2175
Size: 1651 96 68 360
Fragments arranged by size:
1651 360 96 68
SrfI GCCC'GGGC
Cuts at: 0 1747 2175
Size: 1747 428
StyI C'CwwG_G
Cuts at: 0 131 201 740 879 1013 1607 1958 2112
Size: 131 70 539 139 134 594 351 154
Cuts at: 2112 2175
Size: 63
Fragments arranged by size:
594 539 351 154 139 134 131 70
63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 208 997 1739 2161 2175
Size: 208 789 742 422 14
Fragments arranged by size:
789 742 422 208 14
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1246 1278 2175
Size: 1246 32 897
Fragments arranged by size:
1246 897 32
Tth111I GACn'n_nGTC
Cuts at: 0 703 2175
Size: 703 1472
XmaI C'CCGG_G
Cuts at: 0 1649 1745 1813 2175
Size: 1649 96 68 362
Fragments arranged by size:
1649 362 96 68
XmnI GAAnn'nnTTC
Cuts at: 0 301 541 2017 2175
Size: 301 240 1476 158
Fragments arranged by size:
1476 301 240 158
Enzymes that do cut:
AccI AcuI AflIII AhdI AleI AlfI AlwNI ApaI
ApaLI AvaI AvrII BanI BanII BbsI BbvCI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrFI
BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI CspCI
DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NspI PasI PciI PflMI PfoI
PpiI PpuMI PsiI PspOMI PstI PvuII SacI SanDI
SapI SexAI SfcI SmaI SrfI StyI TaqII TstI
Tth111I XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AloI
ApoI AscI AseI AsiSI BaeI BamHI BbeI BcgI
BciVI BclI BfrBI BmtI BpuEI BsaAI BsiWI BsmBI
BspHI BsrDI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I
ClaI DraI DrdI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
NarI NheI NotI NruI NsiI PacI PmeI PmlI
PshAI PspXI PsrI PvuI RsrII SacII SalI SbfI
ScaI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SspI StuI SwaI TatI XbaI XcmI XhoI
ZraI