Restriction map for
pF1KSDA1538
(Linear) (Six-base) MAPSORT of: KIAA1538.seq Check: 5269 from: 1 to: 3039
KIAA1538.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1389 2482 3039
Size: 1389 1093 557
Fragments arranged by size:
1389 1093 557
AatII G_ACGT'C
Cuts at: 0 95 3039
Size: 95 2944
AccI GT'mk_AC
Cuts at: 0 793 2603 3039
Size: 793 1810 436
Fragments arranged by size:
1810 793 436
Acc65I G'GTAC_C
Cuts at: 0 555 1091 3039
Size: 555 536 1948
Fragments arranged by size:
1948 555 536
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 167 508 614 991 1159 2796 3039
Size: 147 20 341 106 377 168 1637 243
Fragments arranged by size:
1637 377 341 243 168 147 106 20
AfeI AGC'GCT
Cuts at: 0 2418 3039
Size: 2418 621
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2080 2375 3039
Size: 2080 295 664
Fragments arranged by size:
2080 664 295
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 56 88 3039
Size: 56 32 2951
Fragments arranged by size:
2951 56 32
AlwNI CAG_nnn'CTG
Cuts at: 0 211 400 531 1393 1762 1861 2103 3039
Size: 211 189 131 862 369 99 242 936
Fragments arranged by size:
936 862 369 242 211 189 131 99
ApaI G_GGCC'C
Cuts at: 0 614 620 886 1774 2880 3039
Size: 614 6 266 888 1106 159
Fragments arranged by size:
1106 888 614 266 159 6
ApoI r'AATT_y
Cuts at: 0 2620 3039
Size: 2620 419
AvaI C'yCGr_G
Cuts at: 0 368 613 886 1173 1539 2069 2264 2374
Size: 368 245 273 287 366 530 195 110
Cuts at: 2374 2431 3039
Size: 57 608
Fragments arranged by size:
608 530 368 366 287 273 245 195
110 57
BanI G'GyrC_C
Cuts at: 0 217 500 555 1091 2041 2174 2380 2422
Size: 217 283 55 536 950 133 206 42
Cuts at: 2422 3039
Size: 617
Fragments arranged by size:
950 617 536 283 217 206 133 55
42
BanII G_rGCy'C
Cuts at: 0 614 620 886 1302 1774 2146 2254 2573
Size: 614 6 266 416 472 372 108 319
Cuts at: 2573 2762 2880 3039
Size: 189 118 159
Fragments arranged by size:
614 472 416 372 319 266 189 159
118 108 6
BbeI G_GCGC'C
Cuts at: 0 221 504 3039
Size: 221 283 2535
Fragments arranged by size:
2535 283 221
BbsI GAAGACnn'nnnn_
Cuts at: 0 269 1147 1201 3039
Size: 269 878 54 1838
Fragments arranged by size:
1838 878 269 54
BbvCI CC'TCA_GC
Cuts at: 0 653 849 2513 3039
Size: 653 196 1664 526
Fragments arranged by size:
1664 653 526 196
BglI GCCn_nnn'nGGC
Cuts at: 0 509 527 577 693 1306 1345 1371 3039
Size: 509 18 50 116 613 39 26 1668
Fragments arranged by size:
1668 613 509 116 50 39 26 18
BlpI GC'TnA_GC
Cuts at: 0 1882 2366 3039
Size: 1882 484 673
Fragments arranged by size:
1882 673 484
Bme1580I G_kGCm'C
Cuts at: 0 614 620 886 1774 2160 2196 2383 2880
Size: 614 6 266 888 386 36 187 497
Cuts at: 2880 3039
Size: 159
Fragments arranged by size:
888 614 497 386 266 187 159 36
6
BmgBI CAC'GTC
Cuts at: 0 2155 3039
Size: 2155 884
BmrI ACTGGGnnnn_n'
Cuts at: 0 221 705 756 2569 3039
Size: 221 484 51 1813 470
Fragments arranged by size:
1813 484 470 221 51
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2550 3039
Size: 2550 489
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 323 397 2010 2048 2057 2524 3039
Size: 323 74 1613 38 9 467 515
Fragments arranged by size:
1613 515 467 323 74 38 9
Bpu10I CC'TnA_GC
Cuts at: 0 653 849 1580 2015 2338 2513 2756 3039
Size: 653 196 731 435 323 175 243 283
Fragments arranged by size:
731 653 435 323 283 243 196 175
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1219 2906 3039
Size: 1219 1687 133
Fragments arranged by size:
1687 1219 133
BsaI GGTCTCn'nnnn_
Cuts at: 0 1108 2521 3039
Size: 1108 1413 518
Fragments arranged by size:
1413 1108 518
BsaAI yAC'GTr
Cuts at: 0 894 918 954 3039
Size: 894 24 36 2085
Fragments arranged by size:
2085 894 36 24
BsaHI Gr'CG_yC
Cuts at: 0 92 218 501 845 3039
Size: 92 126 283 344 2194
Fragments arranged by size:
2194 344 283 126 92
BsaWI w'CCGG_w
Cuts at: 0 2714 3039
Size: 2714 325
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 56 86 1075 1105 2937 2952 2967 2982
Size: 56 30 989 30 1832 15 15 15
Cuts at: 2982 3039
Size: 57
Fragments arranged by size:
1832 989 57 56 30 30 15 15
15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 249 252 255 285 1562 1678 1843 1906
Size: 249 3 3 30 1277 116 165 63
Cuts at: 1906 1915 1924 1936 1939 1951 1954 1957 1960
Size: 9 9 12 3 12 3 3 3
Cuts at: 1960 1969 2127 2463 2581 3039
Size: 9 158 336 118 458
Fragments arranged by size:
1277 458 336 249 165 158 118 116
63 30 12 12 9 9 9 3
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 393 567 628 801 808 1428 1605 1623
Size: 393 174 61 173 7 620 177 18
Cuts at: 1623 2117 2130 2145 2783 3039
Size: 494 13 15 638 256
Fragments arranged by size:
638 620 494 393 256 177 174 173
61 18 15 13 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2635 3039
Size: 2635 404
BsiEI CG_ry'CG
Cuts at: 0 2138 2649 3039
Size: 2138 511 390
Fragments arranged by size:
2138 511 390
BsiHKAI G_wGCw'C
Cuts at: 0 682 893 2506 3039
Size: 682 211 1613 533
Fragments arranged by size:
1613 682 533 211
Bsp1286I G_dGCh'C
Cuts at: 0 614 620 682 886 893 1302 1774 2146
Size: 614 6 62 204 7 409 472 372
Cuts at: 2146 2160 2196 2254 2383 2506 2573 2762 2880
Size: 14 36 58 129 123 67 189 118
Cuts at: 2880 3039
Size: 159
Fragments arranged by size:
614 472 409 372 204 189 159 129
123 118 67 62 58 36 14 7
6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1079 1389 1874 2305 2482 3039
Size: 1079 310 485 431 177 557
Fragments arranged by size:
1079 557 485 431 310 177
BsrBI CCG'CTC
Cuts at: 0 1886 2169 2370 2769 2827 3039
Size: 1886 283 201 399 58 212
Fragments arranged by size:
1886 399 283 212 201 58
BsrDI GCAATG_nn'
Cuts at: 0 990 1355 2396 3039
Size: 990 365 1041 643
Fragments arranged by size:
1041 990 643 365
BsrFI r'CCGG_y
Cuts at: 0 1335 2078 3039
Size: 1335 743 961
Fragments arranged by size:
1335 961 743
BsrGI T'GTAC_A
Cuts at: 0 987 3039
Size: 987 2052
BssSI C'ACGA_G
Cuts at: 0 993 3039
Size: 993 2046
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2935 3039
Size: 2935 104
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1722 2090 3039
Size: 1722 368 949
Fragments arranged by size:
1722 949 368
BstZ17I GTA'TAC
Cuts at: 0 2604 3039
Size: 2604 435
Bsu36I CC'TnA_GG
Cuts at: 0 2984 3039
Size: 2984 55
BtgI C'CryG_G
Cuts at: 0 75 575 1161 1449 1646 1658 1684 2241
Size: 75 500 586 288 197 12 26 557
Cuts at: 2241 2346 3039
Size: 105 693
Fragments arranged by size:
693 586 557 500 288 197 105 75
26 12
BtsI GCAGTG_nn'
Cuts at: 0 445 682 1470 1529 2064 2304 2504 2918
Size: 445 237 788 59 535 240 200 414
Cuts at: 2918 3039
Size: 121
Fragments arranged by size:
788 535 445 414 240 237 200 121
59
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2615 2650 3039
Size: 2615 35 389
Fragments arranged by size:
2615 389 35
DraIII CAC_nnn'GTG
Cuts at: 0 897 3039
Size: 897 2142
DrdI GACnn_nn'nnGTC
Cuts at: 0 27 3039
Size: 27 3012
EaeI y'GGCC_r
Cuts at: 0 578 912 1487 1649 1764 2080 2135 2646
Size: 578 334 575 162 115 316 55 511
Cuts at: 2646 2822 3039
Size: 176 217
Fragments arranged by size:
578 575 511 334 316 217 176 162
115 55
EagI C'GGCC_G
Cuts at: 0 2135 2646 3039
Size: 2135 511 393
Fragments arranged by size:
2135 511 393
EarI CTCTTCn'nnn_
Cuts at: 0 88 258 273 291 324 330 590 967
Size: 88 170 15 18 33 6 260 377
Cuts at: 967 1913 1928 2969 3039
Size: 946 15 1041 70
Fragments arranged by size:
1041 946 377 260 170 88 70 33
18 15 15 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1014 1060 3039
Size: 1014 46 1979
Fragments arranged by size:
1979 1014 46
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 167 323 397 508 614 991 1159
Size: 147 20 156 74 111 106 377 168
Cuts at: 1159 2010 2048 2057 2524 2796 3039
Size: 851 38 9 467 272 243
Fragments arranged by size:
851 467 377 272 243 168 156 147
111 106 74 38 20 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 535 3039
Size: 535 2504
EcoO109I rG'GnC_Cy
Cuts at: 0 171 372 528 663 882 883 1502 1747
Size: 171 201 156 135 219 1 619 245
Cuts at: 1747 1770 1771 1994 2279 2544 2876 2877 3039
Size: 23 1 223 285 265 332 1 162
Fragments arranged by size:
619 332 285 265 245 223 219 201
171 162 156 135 23 1 1 1
HaeII r_GCGC'y
Cuts at: 0 221 437 504 818 1848 2420 3039
Size: 221 216 67 314 1030 572 619
Fragments arranged by size:
1030 619 572 314 221 216 67
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1945 1977 3039
Size: 1945 32 1062
Fragments arranged by size:
1945 1062 32
HindIII A'AGCT_T
Cuts at: 0 723 2519 3039
Size: 723 1796 520
Fragments arranged by size:
1796 723 520
KasI G'GCGC_C
Cuts at: 0 217 500 3039
Size: 217 283 2539
Fragments arranged by size:
2539 283 217
KpnI G_GTAC'C
Cuts at: 0 559 1095 3039
Size: 559 536 1944
Fragments arranged by size:
1944 559 536
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1345 2071 3039
Size: 1345 726 968
Fragments arranged by size:
1345 968 726
MscI TGG'CCA
Cuts at: 0 580 1651 3039
Size: 580 1071 1388
Fragments arranged by size:
1388 1071 580
MslI CAynn'nnrTG
Cuts at: 0 983 3039
Size: 983 2056
MspA1I CmG'CkG
Cuts at: 0 69 247 400 475 628 1633 1782 2134
Size: 69 178 153 75 153 1005 149 352
Cuts at: 2134 2149 2332 2483 3039
Size: 15 183 151 556
Fragments arranged by size:
1005 556 352 183 178 153 153 151
149 75 69 15
NaeI GCC'GGC
Cuts at: 0 2080 3039
Size: 2080 959
NarI GG'CG_CC
Cuts at: 0 218 501 3039
Size: 218 283 2538
Fragments arranged by size:
2538 283 218
NcoI C'CATG_G
Cuts at: 0 575 1449 1646 3039
Size: 575 874 197 1393
Fragments arranged by size:
1393 874 575 197
NgoMIV G'CCGG_C
Cuts at: 0 2078 3039
Size: 2078 961
NspI r_CATG'y
Cuts at: 0 2362 3039
Size: 2362 677
PflMI CCAn_nnn'nTGG
Cuts at: 0 211 1393 1690 3039
Size: 211 1182 297 1349
Fragments arranged by size:
1349 1182 297 211
PfoI T'CCnGG_A
Cuts at: 0 374 2540 3039
Size: 374 2166 499
Fragments arranged by size:
2166 499 374
PmlI CAC'GTG
Cuts at: 0 894 918 3039
Size: 894 24 2121
Fragments arranged by size:
2121 894 24
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 56 88 3039
Size: 56 32 2951
Fragments arranged by size:
2951 56 32
PpuMI rG'GwC_Cy
Cuts at: 0 372 1502 1747 2544 3039
Size: 372 1130 245 797 495
Fragments arranged by size:
1130 797 495 372 245
PshAI GACnn'nnGTC
Cuts at: 0 1120 1868 3039
Size: 1120 748 1171
Fragments arranged by size:
1171 1120 748
PspOMI G'GGCC_C
Cuts at: 0 610 616 882 1770 2876 3039
Size: 610 6 266 888 1106 163
Fragments arranged by size:
1106 888 610 266 163 6
PstI C_TGCA'G
Cuts at: 0 15 749 837 1590 1638 1787 3039
Size: 15 734 88 753 48 149 1252
Fragments arranged by size:
1252 753 734 149 88 48 15
PvuII CAG'CTG
Cuts at: 0 247 400 475 628 1782 2332 3039
Size: 247 153 75 153 1154 550 707
Fragments arranged by size:
1154 707 550 247 153 153 75
SanDI GG'GwC_CC
Cuts at: 0 1502 1747 3039
Size: 1502 245 1292
Fragments arranged by size:
1502 1292 245
SbfI CC_TGCA'GG
Cuts at: 0 837 3039
Size: 837 2202
SfcI C'TryA_G
Cuts at: 0 11 429 476 745 794 833 1287 1586
Size: 11 418 47 269 49 39 454 299
Cuts at: 1586 1634 1783 3039
Size: 48 149 1256
Fragments arranged by size:
1256 454 418 299 269 149 49 48
47 39 11
SfoI GGC'GCC
Cuts at: 0 219 502 3039
Size: 219 283 2537
Fragments arranged by size:
2537 283 219
SmaI CCC'GGG
Cuts at: 0 370 615 1175 2266 2376 2433 3039
Size: 370 245 560 1091 110 57 606
Fragments arranged by size:
1091 606 560 370 245 110 57
SmlI C'TyrA_G
Cuts at: 0 1234 2921 3039
Size: 1234 1687 118
Fragments arranged by size:
1687 1234 118
SphI G_CATG'C
Cuts at: 0 2362 3039
Size: 2362 677
SrfI GCCC'GGGC
Cuts at: 0 615 1175 3039
Size: 615 560 1864
Fragments arranged by size:
1864 615 560
StyI C'CwwG_G
Cuts at: 0 575 1449 1646 1670 2312 2408 3039
Size: 575 874 197 24 642 96 631
Fragments arranged by size:
874 642 631 575 197 96 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 240 1215 1442 1563 1611 3039
Size: 240 975 227 121 48 1428
Fragments arranged by size:
1428 975 240 227 121 48
TatI w'GTAC_w
Cuts at: 0 987 3039
Size: 987 2052
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 356 388 3039
Size: 356 32 2651
Fragments arranged by size:
2651 356 32
Tth111I GACn'n_nGTC
Cuts at: 0 960 3039
Size: 960 2079
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 632 3039
Size: 632 2407
XmaI C'CCGG_G
Cuts at: 0 368 613 1173 2264 2374 2431 3039
Size: 368 245 560 1091 110 57 608
Fragments arranged by size:
1091 608 560 368 245 110 57
ZraI GAC'GTC
Cuts at: 0 93 3039
Size: 93 2946
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AlfI AloI
AlwNI ApaI ApoI AvaI BanI BanII BbeI BbsI
BbvCI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstXI BstZ17I Bsu36I BtgI
BtsI CspCI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII KasI KpnI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI
PstI PvuII SanDI SbfI SfcI SfoI SmaI SmlI
SphI SrfI StyI TaqII TatI TstI Tth111I XcmI
XmaI ZraI
Enzymes that do not cut:
AclI AflII AflIII AgeI AhdI AleI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI
BfrBI BglII BmtI BsaBI BsiWI BsmI BsmBI BspEI
BspHI BssHII BstBI BstEII BstYI BtgZI ClaI DraI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HincII
HpaI MfeI MluI NdeI NheI NotI NruI NsiI
PacI PasI PciI PmeI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SapI ScaI SexAI SfiI
SgfI SgrAI SnaBI SpeI SspI StuI SwaI XbaI
XhoI XmnI